TY - JOUR A1 - Borzekowski, Antje A1 - Drewitz, T. A1 - Keller, Julia A1 - Pfeifer, Dietmar A1 - Kunte, Hans-Jörg A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Biosynthesis and characterization of zearalenone-14-sulfate, zearalenone-14-glucoside and zearalenone-16-glucoside using common fungal strains JF - Toxins N2 - Zearalenone (ZEN) and its phase II sulfate and glucoside metabolites have been detected in food and feed commodities. After consumption, the conjugates can be hydrolyzed by the human intestinal microbiota leading to liberation of ZEN that implies an underestimation of the true ZEN exposure. To include ZEN conjugates in routine analysis, reliable standards are needed, which are currently not available. Thus, the aim of the present study was to develop a facilitated biosynthesis of ZEN-14-sulfate, ZEN-14-glucoside and ZEN-16-glucoside. A metabolite screening was conducted by adding ZEN to liquid fungi cultures of known ZEN conjugating Aspergillus and Rhizopus strains. Cultivation conditions and ZEN incubation time were varied. All media samples were analyzed for metabolite formation by HPLC-MS/MS. In addition, a consecutive biosynthesis was developed by using Fusarium graminearum for ZEN biosynthesis with subsequent conjugation of the toxin by utilizing Aspergillus and Rhizopus species. ZEN-14-sulfate (yield: 49%) is exclusively formed by Aspergillus oryzae. ZEN-14-glucoside (yield: 67%) and ZEN-16-glucoside (yield: 39%) are formed by Rhizopus oryzae and Rhizopus oligosporus, respectively. Purities of ≥73% ZEN-14-sulfate, ≥82% ZEN-14-glucoside and ≥50% ZEN-16-glucoside were obtained by 1H-NMR. In total, under optimized cultivation conditions, fungi can be easily utilized for a targeted and regioselective synthesis of ZEN conjugates. KW - Mycotoxin KW - Zearalenone KW - Conjugate KW - Biosynthesis KW - Fusarium KW - Aspergillus KW - Rhizopus PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-444246 DO - https://doi.org/10.3390/toxins10030104 SN - 2072-6651 VL - 10 IS - 3 SP - Article 104, 1 EP - 15 PB - MDPI CY - Basel AN - OPUS4-44424 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Borzekowski, Antje A1 - Anggriawan, R. A1 - Auliyati, M. A1 - Kunte, Hans-Jörg A1 - Koch, Matthias A1 - Rohn, S. A1 - Karlovsky, P. A1 - Maul, Ronald T1 - Formation of Zearalenone Metabolites in Tempeh Fermentation JF - Molecules N2 - Tempeh is a common food in Indonesia, produced by fungal fermentation of soybeans using Rhizopus sp., as well as Aspergillus oryzae, for inoculation. Analogously, for economic reasons, mixtures of maize and soybeans are used for the production of so-called tempeh-like products. For maize, a contamination with the mycoestrogen zearalenone (ZEN) has been frequently reported. ZEN is a mycotoxin which is known to be metabolized by Rhizopus and Aspergillus species. Consequently, this study focused on the ZEN transformation during tempeh fermentation. Five fungal strains of the genera Rhizopus and Aspergillus, isolated from fresh Indonesian tempeh and authentic Indonesian inocula, were utilized for tempeh manufacturing from a maize/soybean mixture (30:70) at laboratory-scale. Furthermore, comparable tempeh-like products obtained from Indonesian markets were analyzed. Results from the HPLC-MS/MS analyses show that ZEN is intensely transformed into its metabolites alpha-zearalenol (alpha-ZEL), ZEN-14-sulfate, alpha-ZEL-sulfate, ZEN-14-glucoside, and ZEN-16-glucoside in tempeh production. alpha-ZEL, being significantly more toxic than ZEN, was the main metabolite in most of the Rhizopus incubations, while in Aspergillus oryzae fermentations ZEN-14-sulfate was predominantly formed. Additionally, two of the 14 authentic samples were contaminated with ZEN, alpha-ZEL and ZEN-14-sulfate, and in two further samples, ZEN and alpha-ZEL, were determined. Consequently, tempeh fermentation of ZEN-contaminated maize/soybean mixture may lead to toxification of the food item by formation of the reductive ZEN metabolite, alpha-ZEL, under model as well as authentic conditions. KW - Modified mycotoxins KW - Zearalenone sulfate KW - a-zearalenol KW - Food fermentation KW - Rhizopus and Aspergillus oryzae PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-491126 DO - https://doi.org/10.3390/molecules24152697 VL - 24 IS - 15 SP - 2697 PB - MDPI AN - OPUS4-49112 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn A1 - Florian, Camilo A1 - Mezera, Marek A1 - Wasmuth, Karsten A1 - Richter, Anja A1 - Schwibbert, Karin A1 - Krüger, Jörg A1 - Müller, F. A. A1 - Gräf, S. T1 - A brief survey on open questions about laser-induced periodic surface structures N2 - The processing of laser-induced periodic surface structures (LIPSS) represents a simple and robust way for the nanostructuring of solids that allows creating a wide range of surface functionalities featuring applications in optics, tribology, medicine, energy technologies, etc. While the currently available laser and scanner technology already allows surface processing rates at the m2/min level, industrial applications of LIPSS are sometimes hampered by the complex interplay between the nanoscale surface topography and the specific surface chemistry. This typically manifests in difficulties to control the processing of LIPSS and in limitations to ensure the long-term stability of the created surface functions. This presentation aims to identify some unsolved scientific problems related to LIPSS, discusses the pending technological limitations, and sketches the current state of theoretical modelling. Hereby, it is intended to stimulate further research and developments in the field of LIPSS for overcoming these limitations and for supporting the transfer of the LIPSS technology into industry. T2 - E-MRS Spring Meeting 2022 CY - Online meeting DA - 30.05.2022 KW - Laser-induced periodic surface structures (LIPSS) KW - Surface functionalization KW - Industrial applications KW - Biofilm growth PY - 2022 AN - OPUS4-54929 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn A1 - Meissner, Sven A1 - Wasmuth, Karsten A1 - Schwibbert, Karin T1 - Impact of laser-induced periodic surface structures on the bactericidal properties of copper and brass N2 - Surfaces of metallic copper and copper alloys effectively inactivate microorganisms and viruses. However, the exact inactivation mode is still under debate. Main factors are assumed to include direct contact with the metallic surface, influx of Cu(I)/Cu(II) ions and the generation of reactive oxygen species (ROS). Laser-induced periodic surface structures (LIPSS) are frequently reported to act antibacterial, mainly by prevention of bacterial adhesion due to a limited number of possible adhesion points or by increasing the overall surface of intrinsically antibacterial materials. In time-kill experiments with E. coli and S. aureus we analyzed the impact of LIPSS on the toxicity of metallic copper and brass. We also conducted ROS accumulation assays and conclude that the application of LIPSS is not generally straight forward to obtain or improve antibacterial surfaces. Thus, the antibacterial effects of LIPPS. T2 - 2023 Spring Meeting CY - Strasbourg, France DA - 29.05.2023 KW - LIPSS KW - Antimicrobial KW - Reactive oxygen species PY - 2023 AN - OPUS4-58465 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Barkeshli, Mandana A1 - Stephan, Ina A1 - Shevchuk, Ivan A1 - Soltani, Mojtaba T1 - Characteristics of Sizing Materials Used in Persian Medieval Manuscripts: Physical, Optical, Spectral Imaging, and Fungicidal Properties JF - Studies in Conservation N2 - In this study, we investigated the diverse range of materials used for sizing in Iranian paper manuscripts during the Timurid (fifteenth century) to Safavid (sixteenth century) and Qajar (nineteenth century) periods. Our approach combined historical analysis with scientific examination of reconstructed sizings. We reconstructed 15 sizing materials based on identified Persian historical recipes and analysed their physical, optical, and spectral characteristics. Additionally, we assessed their behaviour against the mould fungus Aspergillus flavus. The results revealed distinctive properties for each sizing material, shedding light on their potential applications in paper preservation. Furthermore, our investigation demonstrated variations in hygroscopicity, thickness, grammage, and ash content post-sizing. The sizing materials also exhibited different effects on paper reflectance properties. Additionally, our study revealed insights into the impact of sizing on burnished papers, indicating that the mechanical process of burnishing did not significantly alter the chemical composition or spectral properties of the paper, with only minor changes in brightness observed in specific cases. All tested sizing materials supported varying levels of mould growth, indicating potential implications for paper conservation. Our findings provide valuable insights into the historical practices of Iranian paper sizing and offer practical considerations for the preservation of paper manuscripts. KW - Paper sizings KW - Persian historical recipes KW - Physical and optical characteristics KW - Fungicidal property KW - Spectral imaging PY - 2024 DO - https://doi.org/10.1080/00393630.2024.2342647 SP - 1 EP - 18 PB - Informa UK Limited AN - OPUS4-60159 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Barkeshli, M. A1 - Stephan, Ina ED - Barkeshli, M. ED - Arya, A. ED - Nath, V. ED - Abduraheem, K. T1 - Sizing materials used in Persian manuscripts and their behaviour against the mould fungus Aspergillus flavus T2 - Biodeterioration of cultural property and researches on conservation of heritage buildings - A Festschrift in honour of Padma Shri Dr. O. P. Agrawal N2 - Our earlier study based on historical and scientific analysis showed that Iranians used a considerable range of materials in the sizing process. Unlike many nations who used limited sizing materials to improve the mechanical strength and to smoothen the paper surface, Iranians have used various materials for sizing process from Taimurid (15th century) to Safawid (16th century) and Qajar (19th century) periods. Further to our earlier study on the Sizing Materials used in Persian manuscripts and miniature paintings, scientific analysis was carried out to investigate their behaviour against the mould fungus Aspergillus flavus. In the first stage fourteen different sizing materials that was identified from Persian historical recipes were reconstructed. In the second stage fungicidal property of each sizing samples against Aspergillus flavus fungus were identified that will be presented in this paper. KW - Sizing KW - Materials KW - Persian manuscripts KW - Deterioration KW - Fungus KW - Aspergillus flavus PY - 2023 SN - 978-93-5461-714-0 SP - 3 EP - 20 PB - Daya Publishing House CY - New Delhi AN - OPUS4-58102 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bandow, N. A1 - Aitken, M. D. A1 - Geburtig, Anja A1 - Kalbe, Ute A1 - Piechotta, Christian A1 - Schoknecht, Ute A1 - Simon, Franz-Georg A1 - Stephan, Ina T1 - Using Environmental Simulations to Test the Release of Hazardous Substances from Polymer-Based Products: Are Realism and Pragmatism Mutually Exclusive Objectives? JF - Materials N2 - The potential release of hazardous substances from polymer-based products is currently in the focus of environmental policy. Environmental simulations are applied to expose such products to selected aging conditions and to investigate release processes. Commonly applied aging exposure types such as solar and UV radiation in combination with water contact, corrosive gases, and soil contact as well as expected general effects on polymers and additional ingredients of polymer-based products are described. The release of substances is based on mass-transfer processes to the material surfaces. Experimental approaches to investigate transport processes that are caused by water contact are presented. For tailoring the tests, relevant aging exposure types and release quantification methods must be combined appropriately. Several studies on the release of hazardous substances such as metals, polyaromatic hydrocarbons, flame retardants, antioxidants, and carbon nanotubes from polymers are summarized exemplarily. Differences between natural and artificial exposure tests are discussed and demonstrated for the release of flame retardants from several polymers and for biocides from paints. Requirements and limitations to apply results from short-term artificial environmental exposure tests to predict long-term environmental behavior of polymers are presented. KW - Environmental simulations KW - Polymer-based products KW - Artificial weathering KW - Degradation KW - Leaching KW - Soil contact PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-509310 DO - https://doi.org/10.3390/ma13122709 SN - 1996-1944 VL - 13 IS - 12 SP - Paper 2709, 22 PB - MDPI CY - Basel, Switzerland AN - OPUS4-50931 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - An, Biwen A1 - Shen, Y. A1 - Voordouw, J. A1 - Voordouw, G. ED - Dumas, C. T1 - Halophilic Methylotrophic Methanogens May Contribute to the High Ammonium Concentrations Found in Shale Oil and Shale Gas Reservoirs T2 - Halophilic Methanogenesis in Shale Reservoirs N2 - Flow-back and produced waters from shale gas and shale oil fields contain high ammonium, which can be formed by methanogenic degradation of methylamines into methane and ammonium. Methylamines are added to fracturing fluid to prevent clay swelling or can originate from metabolism of the osmolyte triglycinebetaine (GB). We analyzed field samples from a shale gas reservoir in the Duvernay Formation and from a shale oil reservoir in the Bakken formation in Canada to determine the origin of high ammonium. Fresh waters used to make fracturing fluid, early flow-back waters, and late flow back waters from the shale gas reservoir had increasing salinity of 0.01, 0.58, and 2.66 Meq of NaCl, respectively. Microbial community analyses reflected this fresh water to saline transition with halophilic taxa including Halomonas, Halanaerobium, and Methanohalophilus being increasingly present. Early and late flow-back waters had high ammonium concentrations of 32 and 15 mM, respectively. Such high concentrations had also been found in the Bakken produced waters. Enrichment cultures of Bakken produced waters in medium containing mono, di-, or trimethylamine, or triglycinebetaine (GB) converted these substrates into ammonium (up to 20 mM) and methane. The methylotrophic methanogen Methanohalophilus, which uses methylamines for its energy metabolism and uses GB as an osmolyte, was a dominant community member in these enrichments. Halanaerobium was also a dominant community member that metabolizes GB into trimethylamine, which is then metabolized further by Methanohalophilus. However, the micromolar concentrations of GB measured in shale reservoirs make them an unlikely source for the 1,000-fold higher ammonium concentrations in flow-back waters. This ammonium either originates directly from the reservoir or is formed from methylamines, which originate from the reservoir, or are added during the hydraulic fracturing process. These methylamines are then converted into ammonium and methane by halophilic methylotrophic methanogens, such as Methanohalophilus, present in flow-back waters. KW - Methanogen KW - Oil and gas industry KW - Shale KW - Halophile KW - Corrosion PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-474982 UR - https://www.frontiersin.org/articles/10.3389/fenrg.2019.00023/full DO - https://doi.org/10.3389/fenrg.2019.00023 VL - 7 SP - Article 23, 1 EP - 13 PB - Frontiers Media CY - Frontiers in Energy Research AN - OPUS4-47498 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - An, Biwen Annie A1 - Voordouw, G. ED - An, Biwen Annie T1 - Chapter 4. Microbial Communities Involved in High Salinity Souring in Shale Oil Fields T2 - Oilfield Microbiology N2 - This book chapter provides an overview of the negative impacts of halophilic microorganisms in oil and gas operations. The chapter used a Canadian shale oil reservoir as an case study example to show the high souring and corrosion potential of halophilic microorganisms. KW - Corrosion KW - Halophilic KW - Microorganism KW - Microbial community modelling KW - Oil and gas reservoir KW - Shale KW - Geological formation KW - Oilfield PY - 2019 SN - 13 978-1-138-05775-3 SP - 57 EP - 69 PB - CRC Press CY - Boca Raton ET - 1. AN - OPUS4-49599 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Kunte, Hans-Jörg A1 - Koerdt, Andrea ED - Eibergen, N. ED - Poulassichidis, T. T1 - Microbiologically Influenced Corrosion (MIC) by Halophilic (Salt-Loving) Nitrate and Sulfate-Reducing Microorganisms T2 - Corrosion 2021 N2 - The survey of Canadian shale sites showed a dominance of halophilic microorganisms, including Halomonas (HA). Nitrate-amended incubations of the field samples under high salinity (14.6% NaCl), revealed a dominance of HA (>72%) and an accumulation of nitrite. Nitrite accumulation directly inhibited the growth of SRB, thereby decreasing their souring and corrosion risks. However, accumulated nitrite may also contribute to iron corrosion, which will be tested by using different concentrations of nitrate as an electron acceptor to HA. Different salinities are further tested on HA strains supplemented with iron coupons to determine their effects on iron corrosion rates. HA incubated with separate cultures of corrosive methanogen and SRB were tested to determine whether a positive or adverse effect will occur between them. Lastly, analyses of iron coupons will be conducted using TOF-SIMS, FIB-SEM and EDS for corrosion product characterization T2 - Corrosion 2021 CY - Online Meeting DA - 19.04.2021 KW - MIC KW - Bacteria KW - Halophile KW - Corrosion KW - Environmental condition KW - Korrosion KW - High salinity PY - 2021 UR - https://my.nace.org/PaperTrail/Authors/Submission.aspx?id=2914f145-7f8f-ea11-813a-005056a95a7c SP - Paper C2021-16284, 1 AN - OPUS4-52479 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Koerdt, Andrea A1 - Özcan Sandikcioglu, Özlem A1 - Schreiber, Frank A1 - Kleinbub, Sherin T1 - The study of methanogen-induced microbiologically influenced corrosion under flow conditions N2 - Microbiologically influenced corrosion (MIC) is an expensive but unpredictable problem for the industries. The most well-known culprit for MIC is the sulfate-reducing microorganisms (SRM), such as members from the genus Desulfovibrio. It has been widely accepted that SRM can contribute significantly to MIC through the production of hydrogen sulfide (HS-) or in some cases a direct electron uptake from the metal surface. However, in a real environmental system, SRM is not exclusive and often involved with other microorganisms that may also contribute to MIC, such as methanogens. Methanogenic archaea can produce methane (CH4) using H2+CO2, formate, methylated amines or acetate. Methanogens are highly abundant in the environment and many are found in very extreme conditions, such as high temperature and high salinity. Previous researches have demonstrated that methanogens are capable of MIC, though the specific mechanisms are still under investigation. In the oil and gas industry, methanogens are not considered as the main contributor for MIC since the corrosion rates are often too low. However, the tests for methanogen-induced MIC are usually performed at static conditions, which cannot represent the system accurately. Here, we developed a novel anaerobic system to evaluate the corrosion potential of methanogens under flow conditions. We will use the Methanococcus maripaludis KA1 strain, which was isolated from a crude oil tank, as the organism of interest. A separate system for Desulfovibrio alaskensis will be established for corrosion rate comparisons. Furthermore, we will study the synergistic effects of M. maripaludis and D. alaskensis on MIC under flow. T2 - Eurocorr 2018 CY - Krakow, Poland DA - 09.09.2018 KW - Mikrobiell beeinflusste Korrosion KW - Methanogen KW - Korrosion KW - Microbiologically influenced corrosion KW - Methanogenesis KW - Flow system KW - Model PY - 2018 AN - OPUS4-46012 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - An, Biwen Annie A1 - Kleinbub, Sherin A1 - Özcan Sandikcioglu, Özlem A1 - Koerdt, Andrea T1 - Iron to Gas: Versatile Multiport Flow-Column Revealed Extremely High Corrosion Potential by Methanogen-Induced Microbiologically Influenced Corrosion (Mi-MIC) JF - Bioleaching and Biocorrosion: Advances in Interfacial Processes N2 - Currently, sulfate-reducing bacteria (SRB) is regarded as the main culprit of microbiologically influenced corrosion (MIC), mainly due to the low reported corrosion rates of other microorganisms. For example, the highest reported corrosion rate for methanogens is 0.065 mm/yr. However, by investigating methanogen-induced microbiologically influenced corrosion (Mi-MIC) using an in-house developed versatile multiport flow test column, extremely high corrosion rates were observed. We analyzed a large set of carbon steel beads, which were sectionally embedded into the test columns as substrates for iron-utilizing methanogen Methanobacterium IM1. After 14 days of operation using glass beads as fillers for section separation, the highest average corrosion rate of Methanobacterium IM1 was 0.2 mm/yr, which doubled that of Desulfovibrio ferrophilus IS5 and Desulfovibrio alaskensis 16109 investigated at the same conditions. At the most corroded region, nearly 80% of the beads lost 1% of their initial weight (fast-corrosion), resulting in an average corrosion rate of 0.2 mm/yr for Methanobacterium IM1-treated columns. When sand was used as filler material to mimic sediment conditions, average corrosion rates for Methanobacterium IM1 increased to 0.3 mm/yr (maximum 0.52 mm/yr) with over 83% of the beads having corrosion rates above 0.3 mm/yr. Scanning electron images of metal coupons extracted from the column showed methanogenic cells were clustered close to the metal surface. Methanobacterium IM1 is a hydrogenotrophic methanogen with higher affinity to metal than H2. Unlike SRB, Methanobacterium IM1 is not restricted to the availability of sulfate concentration in the environment. Thus, the use of the multiport flow column provided a new insight on the corrosion potential of methanogens, particularly in dynamic conditions, that offers new opportunities for monitoring and development of mitigation strategies. Overall, this study shows under certain conditions methanogenic archaea can cause higher corrosion than SRB, specific quantifications, i.e., maximum, average, and minimum corrosion rates can be determined, and that spatial statistical evaluations of MIC can be carried out. KW - Microbiologically influenced corrosion KW - Methanogen KW - Methane KW - Biocorrosion KW - Flow system KW - Modeling KW - Multiport PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-506084 DO - https://doi.org/10.3389/fmicb.2020.00527 VL - 11 SP - Article 527 PB - Frontiers in microbiology AN - OPUS4-50608 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - An, Biwen Annie A1 - Deland, Eric A1 - Sobol, Oded A1 - Yao, Jizheng A1 - Skovhus, T. L. A1 - Koerdt, Andrea T1 - The differences in the corrosion product compositions of Methanogen-induced microbiologically influenced corrosion (Mi-MIC) between static and dynamic growth conditions JF - Corrosion Science N2 - Currently, corrosion rates (CR) and/or corrosion products (CP) obtained for methanogen-induced microbiologically influenced corrosion (Mi-MIC) on carbon steel are mainly analyzed from static-incubations. By using a multiport-flow-column, much higher CRs (0.72 mm/yr) were observed, indicating static-incubations are not suitable for determining the corrosive potential of Mi-MIC. With the combination of various analytical methods (ToF-SIMS/SEM-EDS/SEM-FIB) and contrary to previously published data, we observed that CPs contained phosphorus, oxygen, magnesium, calcium and iron but lacked carbon-related species (e.g. siderite). Overall, siderite nucleation is disrupted by methanogens, as they convert aqueous bicarbonate into carbon dioxide for methanogenesis resulting in increased localized corrosion. KW - Carbon steel KW - Modelling studies KW - SIMS KW - SEM KW - Reactor conditions KW - Microbiologically influenced corrosion PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-517632 DO - https://doi.org/10.1016/j.corsci.2020.109179 SN - 0010-938X VL - 180 SP - 9179 PB - Elsevier AN - OPUS4-51763 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Koerdt, Andrea T1 - Investigating and modelling MIC using in-house developed flow system (Hi-Tension) N2 - Microbiologically influenced corrosion is a multidisciplinary research area. To develop successful mitigation strategies, expertise from the industry and research institutes are essential. In Department 4.1, we developed an innovative laboratory flow model (Hi-Tension) that allows effective monitoring of MIC under both standard and non-standard conditions. The flow model allows flexibility with material selection, flow rates, temperature and other environmental parameters changes. Furthermore, the flow model allows integration of electrochemical measurements using microsensors, providing a comprehensive view of corrosion at the biofilm level. Currently, initial results indicate corrosion in the flow model is significantly higher than that of standard laboratory set ups, i.e. static incubations, particularly for methane-producing microorganisms. T2 - Departmental Meeting with Helmotz Dresden CY - BAM, Berlin, Germany DA - 04.11.2019 KW - MIC KW - Corrosion KW - FIB/SEM KW - Corrosion products KW - Hi-Tension KW - Flow Model KW - Modelling PY - 2019 AN - OPUS4-49417 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Koerdt, Andrea A1 - Kleinbub, Sherin T1 - Microbial modelling of sulfate-reducing bacteria (SRB) and methanogenic archaea (ME) using iron N2 - Sulfate reducing bacteria (SRB) and methanogenic archaea (MA) are commonly found in the oil and gas environments. The formation of hydrogen sulfide (HS-) is particularly concerning for the petroleum industry due to its corrosiveness. However, the activities of SRB are limited to the concentration of sulfate present in the environment, whereas methanogens can utilize substrates such as H¬2 for methanogenesis. MA is commonly found in sulfate-free environments, such as deep sediments, and are known to form interspecies electron transfer relationships with SRB. Recently, SRB and MA capable of microbiologically influenced corrosion (MIC) by using elemental iron as a direct electron source (EMIC) have gained increased attention. On the iron surface, EMIC-SRB can outcompete EMIC-MA in the presence of sulfate, but this changes as sulfate depletes. The formation of FeS on the metal surface can be further utilized by MA for methanogenesis as it provides a conductive path. However, the possible kinetics involved of the overall process are currently unknown. We obtained a co-culture of EMIC-SRB and EMIC-MA to investigate the growth rates and electrical potential changes under different environmental conditions, including changes in pH, temperature and salinity. Results indicate that under neutral conditions and using iron as the sole substrate, methane production (up to 5 mM) starts after sulfate was depleted. Electrochemical measurements will be conducted on the co-culture under different conditions to determine the changes in the electrical potential in correlation with the sulfate and methane concentration. Fluorescence and electron microscope images of the biofilm structure will be used to visualize cell distribution and morphology. This study embarks the first step of understanding the relationship between EMIC-SRB and EMIC-MA. Such knowledge is important for the field of microbial electrophysiology and can be further explored for industrial applications. T2 - 7th International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-7) CY - Halifax, Canada DA - 18.06.2019 KW - MIC KW - Corrosion KW - FIB/SEM KW - Corrosion products KW - Bacteria KW - Archaea KW - Iron PY - 2019 AN - OPUS4-49420 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Koerdt, Andrea A1 - Brüggemann, Kristin A1 - Wurzler, Nina A1 - Kleinbub, Sherin A1 - Özcan Sandikcioglu, Özlem T1 - Effect of biocides on highly corrosive methanogenic Archaea N2 - Biocide mitigation strategies of microbiologically influenced corrosion (MIC) in the oil and gas industry have been primarily used to eliminate the growths of sulfate-reducing microorganisms (SRM). However, methanogenic Archaea (MA) can also be highly corrosive by using iron as an electron source for methanogenesis. Because of the fundamental physiological differences between archaea and bacteria, responses of MA towards SRM-specific biocides cannot be deduced using SRM. Due to the lack of information available on the effect of biocides on corrosive MA, we selected THPS, glutaraldehyde, nitrate and perchlorate to compare against corrosive SRM. Preliminary results showed that at low concentrations of THPS (0-10 ppm), growth of MA was not affected, methane production and corrosion rates (0.1 mm/yr) were comparable between the different THPS concentrations. On the contrary, the SRM strain showed decreased corrosion rates (0.18 mm/yr to 0.03 mm/yr) with increasing THPS concentrations. Further corrosion tests including electrochemical measurements of different biocides on the growth of MA and SRM will be conducted. Such knowledge not only provide important insights on the physiological response of MA to biocides but also contribute to more effective mitigation strategies that can be both economic and environmentally beneficial. T2 - 25th Annual Reservoir Microbiology Forum CY - London, UK DA - 20.11.2019 KW - Corrosion KW - Methanogen KW - Microorganism KW - Biocide PY - 2019 AN - OPUS4-49788 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Kleinbub, Sherin A1 - Schreiber, Frank A1 - Koerdt, Andrea T1 - Investigating the effects of biocides and corrosion inhibitors on corrosive methanogens N2 - Microbiologically influenced corrosion (MIC) is the deterioration of metals due to the metabolic activities of microorganisms. Microorganisms can take electrons directly from the metal surface (EMIC) thereby causing corrosion. Well known culprits of EMIC are: sulfate-reducing bacteria (SRB), acetogens and methanogens. Our aims - Develop a novel flow system to study MIC by methanogens to mimic industrial Environments - Investigate the inhibitory concentrations of biocides targeting SRB on corrosive methanogenic strains - Investigate the inhibitory effects of corrosion inhibitors on methanogens - Compare the inhibitory concentrations to SRB T2 - BAM meeting CY - BAM, Berlin, Germany DA - 06.06.2018 KW - MIC projekt KW - Mikrobiell beeinflusste Korrosion KW - Microbiologically influenced corrosion KW - Korrosion KW - Corrosion KW - Material degradation KW - Biocide PY - 2018 AN - OPUS4-46010 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Annie Biwen A1 - Deland, Eric A1 - Kleinbub, Sherin A1 - Koerdt, Andrea T1 - Standardization of MIC laboratory testing: with a special focus on corrosive methanogens N2 - Microbiologically influenced corrosion (MIC) is a highly unpredictable process involving a complex group of microorganisms, including sulfate-reducing bacteria and methanogens. Standard laboratory MIC testing using static serum bottle enrichments is an easy but limited method, offering poor resolution on the biomineralization process of corrosion products. An example of this is the presumed corrosion product siderite by corrosive methanogens (Mi-MIC). Previous publications reported siderite was the sole corrosion product of M. maripaludis using metal coupons incubated under stationary conditions. However, the formation of siderite is closely related to the surrounding environmental conditions, i.e. pH, CO2 concentration, flow and temperature. Thus, siderite as the sole corrosion product of Mi-MIC remain inconclusive and questionable. To study Mi-MIC effectively, a novel versatile multiport flow-column corrosion monitoring system (MFC) was developed. MFC allows sectional corrosion rate determination under flow conditions using different types of material, inoculum and packing material. MFC offers great flexibility, ease of operation and accurate corrosion measurements that can be combined with many other techniques. Using MFC, we studied multiple strains of methanogens and compared it with sulfate-reducing bacteria under neutral and low pH conditions. It was revealed by MFC that corrosive methanogens have equally high corrosion potential as sulfate-reducing bacteria. Additionally, siderite is not the dominant nor sole corrosion product of Mi-MIC. Thus, effective corrosion monitoring and establishing standard laboratory practices, i.e. incorporating MFC as part of regular testing process, will provide deeper understanding of MIC. This will allow further microbial electrophysiology understandings, contributing to effective mitigation strategy development. T2 - EUROPEAN MIC NETWORK WEBINAR CY - Online meeting DA - 19.05.2020 KW - MIC KW - Methanogen KW - FIB/SEM KW - Corrosion products KW - Microbiologically influenced corrosion KW - Flow Model KW - Modelling KW - Korrosion PY - 2020 AN - OPUS4-51554 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -