TY - CONF A1 - Kjaervik, Marit A1 - Dietrich, P. A1 - Thissen, A. A1 - Schwibbert, Karin T1 - Surface characterisation of biological samples by near-ambient pressure XPS N2 - A presentation held for the seminar "Ausgewählte analytische Methoden der Physik" hosted by Prof. Birgit Kanngießer at TU Berlin. The first part focus on depth-dependent XPS-measurements (XPS, synchrotron HAXPES) to obtain a concentration profile of iodine in an artificial biofilm. In the second part, NAP-XPS measurements of various bacterial samples are presented. T2 - Forschungsseminar "Ausgewählte analytische Methoden der Physik", TU Berlin CY - Berlin, Germany DA - 17.07.2018 KW - Biofilms KW - Alginate KW - Agarose KW - HAXPES KW - NAP-XPS PY - 2018 AN - OPUS4-45505 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Oberpaul, M. A1 - Spohn, M. A1 - Fracowiak, J. A1 - Plarre, Rüdiger A1 - McMahon, Dino Peter A1 - Hammann, P. A1 - Vilcinskas, A. A1 - Gläser, J. T1 - Exploiting termite nest microbiomes for antibiotic discovery by using an ultra-high throughput Microfluidics/FACS driven pipeline combined with a microtiter plate based cultivation strategy N2 - Infections with multi-resistant Gram negative pathogens are a major threat to our health system. In order to serve the needs in antibiotics development we selected untapped bioresources and implemented high throughput approaches suitable for the discovery of strains producing antibiotics with anti-Gram negative activity. Our approaches rely on the hypothesis that Termite associated bacteria are likely to produce potent antibiotics to defend their hosts against entomopathogenic microorganisms. Termite nests and guts harbor suitable, highly diverse microbiomes in which bacterial taxa are present known to potentially produce natural compounds. In a first step the diversity of Coptotermes species nest microbiomes was assessed carefully by using 16S rDNA amplicon sequencing on the Illumina MiSeq platform and nest material was selected to retrieve viable cells by using Nycodenz density gradient centrifugation. In order to analyze the diversity of the culturable termite nest microbiome, bacterial cells were either distributed in 384-well plates (approach 1) or encapsulated in small spheric agarose beads by an high throughput microfluidics technique (approach 2). Cultures obtained from approach 1 were scaled-up in 96-well Duetz-systems for characterization of diversity and for rapid supernatant screening using the bioluminescence-labeled E. coli pFU166. The generated droplets of approach 2 simultaneously received a small population of GFP-tagged Gram negative screening cells and were sorted for low fluorescence using FACS. After elimination of redundancy we performed a fast scale-up of active strains. Implementation of this pipeline allows us to prioritize antibiotics producing strains in a ultra-high throughput fashion and by cultivation of broad diversity in our approches. T2 - Annual Conference of the Society for General and Applied Microbiology (VAAM) CY - Wolfsburg, Germany DA - 15.04.18 KW - Biotechnology KW - Termites KW - Anti-microbial effects PY - 2018 AN - OPUS4-44987 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Borzekowski, Antje A1 - Drewitz, T. A1 - Keller, Julia A1 - Pfeifer, Dietmar A1 - Kunte, Hans-Jörg A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Biosynthesis and characterization of zearalenone-14-sulfate, zearalenone-14-glucoside and zearalenone-16-glucoside using common fungal strains JF - Toxins N2 - Zearalenone (ZEN) and its phase II sulfate and glucoside metabolites have been detected in food and feed commodities. After consumption, the conjugates can be hydrolyzed by the human intestinal microbiota leading to liberation of ZEN that implies an underestimation of the true ZEN exposure. To include ZEN conjugates in routine analysis, reliable standards are needed, which are currently not available. Thus, the aim of the present study was to develop a facilitated biosynthesis of ZEN-14-sulfate, ZEN-14-glucoside and ZEN-16-glucoside. A metabolite screening was conducted by adding ZEN to liquid fungi cultures of known ZEN conjugating Aspergillus and Rhizopus strains. Cultivation conditions and ZEN incubation time were varied. All media samples were analyzed for metabolite formation by HPLC-MS/MS. In addition, a consecutive biosynthesis was developed by using Fusarium graminearum for ZEN biosynthesis with subsequent conjugation of the toxin by utilizing Aspergillus and Rhizopus species. ZEN-14-sulfate (yield: 49%) is exclusively formed by Aspergillus oryzae. ZEN-14-glucoside (yield: 67%) and ZEN-16-glucoside (yield: 39%) are formed by Rhizopus oryzae and Rhizopus oligosporus, respectively. Purities of ≥73% ZEN-14-sulfate, ≥82% ZEN-14-glucoside and ≥50% ZEN-16-glucoside were obtained by 1H-NMR. In total, under optimized cultivation conditions, fungi can be easily utilized for a targeted and regioselective synthesis of ZEN conjugates. KW - Mycotoxin KW - Zearalenone KW - Conjugate KW - Biosynthesis KW - Fusarium KW - Aspergillus KW - Rhizopus PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-444246 DO - https://doi.org/10.3390/toxins10030104 SN - 2072-6651 VL - 10 IS - 3 SP - Article 104, 1 EP - 15 PB - MDPI CY - Basel AN - OPUS4-44424 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Plarre, Rüdiger T1 - Eine kleine Natur- und Kulturgeschichte der Kleidermotte – nebst Möglichkeiten ihrer biologischen Bekämpfung N2 - Zu den unerwünschten Gästen im Museum oder im Haushalt gehört zweifelsohne die Kleidermotte Tineola bisselliella. Es sind die Larven dieses Kleinschmetterlings, die die Schäden verursachen. Neben der konventionellen Schadensabwehr besteht die Möglichkeit, Schlupfwespen zur Bekämpfung der Larven einzusetzen. Der Vortrag rekonstruiert die Natur- und Kulturgeschichte der Kleidermotte anhand ökologischer Studien und historischen Indizien. T2 - Konservierungswissenschaft im Dialog Rathgen Forschungslabor CY - Berlin, Germany DA - 30.01.2018 KW - IPM KW - Museum KW - Biologische Bekämpfung KW - Kulturgut PY - 2018 AN - OPUS4-43988 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Plarre, Rüdiger A1 - Busweiler, Sabine T1 - Erneute Antennen-Anomalie beim Hausbockkäfer Hylotrupes bajulus (Coleoptera: Cerambycidae) in der F3-Generation JF - Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin N2 - Second occurrence of aberrant antenna in house longhorn beetle Hylotrupes bajulus (Coleoptera: Cerambycidae) in the F3 generation. In 2016, the house longhorn beetle (Hylotrupes bajulus) breeding culture of the Federal Institute for Materials Research and Testing (BAM) in Berlin produced a second female beetle with a unique malformed right antenna similar to the one previously reported. As in the first case the flagellum following the pedical was bifurcated. This time, however, both branches were of similar length with 7 and 8 flagellomeres, respectively. The left antenna was normally developed with 11 antennal segments (scape, pedicel and 9 flagellomeres). This second female stands as F3-generation in direct maternal line to the previous one. A heritable cause for the malformation is likely. KW - Hausbockkäfer KW - Entwicklung KW - Vererbung PY - 2018 SN - 0037-5942 VL - Neue Folge 53 SP - 101 EP - 105 PB - Gesellschaft Naturforschender Freunde zu Berlin CY - Berlin AN - OPUS4-44052 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Plarre, Rüdiger T1 - Termiten in Deutschland - wo gibt es denn so was? JF - Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin N2 - Rare occurrences of termites in Germany. Termites are typical elements of the tropical and subtropical insect fauna. In Europe, their most northern natural range is southern France, Italy and the South-Balkan region. Only occasionally are termites found in Germany. Their occurrence is always very closely associated with the synanthropic environment. Drywood termites like Cryptotermes brevis or Kalotermis flavicollis were accidentally imported with furniture, wooden artefacts or plant material into homes or indoor arboretums. Similar pathways of termite introduction are described for Coptotermes gestroi and Reticulitermes sp. into tropical botanical gardens and tropical theme parks. Although termites were introduced to Germany several times, successful spreading or long term establishment never occurred due to climatic barriers, namely cold winter temperatures. Only Reticulitermes flavipes formed sustainable colonies in the city of Hamburg for several decades. Originating from the eastern United States of America, this species was introduced in the 1930s with infested wood through the sea port. Small subterranean colonies could survive the winter in areas where pipes of the central heating system kept the ground from freezing. Control efforts of this potentially invasive species of economic importance started early on but failed until modern bait techniques were applied in the year 2000. Currently, termites have not been detected in Hamburg for the last five years. KW - Holzschutz KW - Termiten KW - Neozoon PY - 2018 SN - 0037-5942 VL - Neue Folge 53 SP - 83 EP - 90 PB - Gesellschaft Naturforschender Freunde zu Berlin CY - Berlin AN - OPUS4-44055 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kjaervik, Marit A1 - Schwibbert, Karin A1 - Unger, Wolfgang A1 - Thissen, A. A1 - Dietrich, P. T1 - Progress on characterisation of biofilms by NAP-XPS N2 - Progress talk on characterisation of biofilms by NAP-XPS in the framework of the MetVBadBugs EURAMET-project T2 - MetVBadBugs 24 M project meeting CY - Turin, Italy DA - 06.02.2018 KW - Biofilms KW - E. coli KW - Alginate KW - NAP-XPS PY - 2018 AN - OPUS4-44065 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Palma-Onetto, V. A1 - Hoskova, K. A1 - Krizkova, B. A1 - Krejcirova, R. A1 - Pflegerova, J. A1 - Bubenickova, F. A1 - Plarre, Rüdiger A1 - Dahlsjo, C. A. L. A1 - Dahlsjo, J. A1 - Bourguignon, T. A1 - Sillam-Dusses, D. A1 - Sobotnik, J. T1 - The labral gland in termites: evolution and function JF - Biological journal of the Linnean Society N2 - The evolutionary success of termites has been driven largely by a complex communication system operated by a rich set of exocrine glands. As many as 20 different exocrine organs are known in termites. While some of these organs are relatively well known, only anecdotal observations exist for others. One of the exocrine organs that has received negligible attention so far is the labral gland. In this study, we examined the structure and ultrastructure of the Labrum in soldiers of 28 termite species. We confirm that the labral gland is present in all termite species, and comprises two secretory regions located on the ventral side of the labrum and the dorso-apical part of the hypopharynx. The Labrum of Neoisoptera has a hyaline tip, which was secondarily lost in Nasutitermitinae, Microcerotermes and species with snapping soldiers. The epithelium of the gland generally consists of class 1 secretory cells, with an addition of class 3 secretory cells in some species. A common feature of the secretory cells is the abundance of smooth endoplasmic reticulum, an organelle known to produce lipidic and often volatile secretions. Our observations suggest that the labral gland is involved in communication rather than defence as previously suggested. Our study is the first to provide a comprehensive picture of the structure of the labral gland in soldiers across all Termite taxa. KW - Exocrine gland KW - Hypopharynx KW - Labrum KW - Termitoidae KW - Ultrastructure KW - Isoptera PY - 2018 DO - https://doi.org/10.1093/biolinnean/blx162 SN - 0024-4066 SN - 1095-8312 VL - 126 IS - 3 SP - 587 EP - 597 PB - Wiley-Blackwell CY - Oxford AN - OPUS4-44666 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Yalçin, M. A1 - Taşçioğlu, C. A1 - Plarre, Rüdiger A1 - Akçay, Ç. A1 - Busweiler, Sabine T1 - Investigation of natural durability of some native and exotic wood species against Hylotrupes bajulus (Cerambycidae) and Anobium punctatum (Anobiidae) JF - Journal of Forestry Faculty of Kastamonu University N2 - Aim of study: In this study, natural durability of some domestic and foreign wood species against Hylotrupes bajulus and Anobium punctatum larvae were tested on laboratory scale Area of study: This study was conducted at Department of Forest Products Engineering in Duzce University, Turkey and Federal Institute for Materials Research and Testing (BAM), Germany. Material and Methods: Scotch pine (Pinus sylvestris), fir (Abies nordmanniana), spruce (Picea orientalis), cedar (Cedrus libani), poplar (Populus tremula) and beech (Fagus orientalis) woods were used to test H. bajulus larvae (EN 46-1). Alder (Alnus glutinosa), oak (Quercus cerris), poplar (Populus tremula), beech (Fagus orientalis), maple (Acer carpinifolium), ash (Fraxinus angustifolia), teak (Tectona grandis), ayous (Triplochiton scleroxylon), movingui (Distemonanthus benthamianus), dahoma (Piptadeniastrum africanum), iroko (Chlorophora excelsa), bubinga (Guibourtia tessmannii) and sapele (Entandrophragma cylindiricum) woods were used for A. punctatum larvae (EN 49-1). At the end of the experiment, the mortality rates of the larvae were determined and the size and weights of the surviving larvae were measured. Main results: F. orientalis and C. libani were found to be the most resistant wood species against H. bajulus larvae while A. nordmanniana was the least resistant. All tropical wood species and oak and maple from domestic wood species showed 100% mortality rate therefore found to be the most resistant against A. punctatum larvae. The most vulnerable wood species was found to be alder with a 35% mortality. Research highlights: while F. orientalis, C. libani, and P. tremula were found the most resistance wood species against H. bajulus, P. sylvestris and A. nordmanniana were determined as most vulnerable. All tropical wood species and two domestic species (Q. cerris and A. carpinifolium) showed the highest mortality rate as 100%. The least durable domestic wood was determined as alder. KW - Hylotrupes bajulus, Anobium punctatum, natural durability, tropical wood, native wood. PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-446572 DO - https://doi.org/10.17475/kastorman.311971 SN - 1303-2399 SN - 1309-4181 VL - 18 IS - 1 SP - 83 EP - 91 PB - Kastamonu University CY - Kastamonu AN - OPUS4-44657 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koerdt, Andrea A1 - An, Biwen A1 - Kleinbub, Sherin T1 - Microbial corrosion coupled to methanogenesis by strains from different environments N2 - Microbiologically influenced corrosion (MIC) of iron is usually attributed to sulfate-reducing microorganisms, either chemically (formation of hydrogen sulfide) or electrically (direct electron uptake). Methanogenetic Archaea are also known to be involved in iron corrosion, forming a multi-species biofilm on corroding metallic structures. However, mechanistic details and kinetics of the overall process in methanogen-induced MIC are poorly understood. T2 - Beirat Material CY - Berlin, Germany DA - 26.04.2018 KW - Corrosion KW - Methanogens KW - Archaea KW - Implants KW - Biofilm PY - 2018 AN - OPUS4-45112 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -