TY - JOUR A1 - Stohl, Leonie A1 - Tonon, Chiara A1 - Cook, Jake A1 - Gorbushina, Anna A1 - Dehn, Frank A1 - von Werder, Julia T1 - Understanding bioreceptivity of concrete: realistic and accelerated weathering experiments with model subaerial biofilms N2 - Vertical greening systems are a promising solution to the increasing demand for urban green spaces, improving environmental quality and addressing biodiversity loss. This study facilitates the development microbially greened algal biofilm facades, which offer a low maintenance vertical green space. The study focuses on concrete as a widely used building material and explores how physical surface characteristics impact its bioreceptive properties. Concrete samples, produced from the same mix but differing in surface structure, were subjected to a laboratory weathering experiment to assess their bioreceptivity. A novel inoculation method was employed, involving a single initial inoculation with either alga ( Jaagichlorella sp.) alone, or a model biofilm consisting of a combination of the alga ( Jaagichlorella sp.) with a fungus ( Knufia petricola). The samples underwent four months of weathering in a dynamic laboratory setup irrigated with deionized water to observe subaerial biofilm attachment and growth. The formation of subaerial biofilms was monitored with high resolution surface imaging, colorimetric measurements and Imaging Pulse Amplitude Modulated Fluorometry (Imaging PAM-F), with Imaging PAM-F proving the most effective. Statistical analysis revealed that by impacting surface pH value and water retention capability, surface structures significantly influence microbial growth and that the concrete’s bioreceptivity can be influenced through thoughtful design of the materials surface. The inoculation of algae combined with a fungus facilitated the formation of a stable subaerial biofilm, enabling algae to colonize a surface structure that it could not colonize alone. This finding highlights the importance of modelling synergistic interactions present in natural biofilms. KW - Bioreceptivity KW - Concrete KW - Biofilm PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-653105 DO - https://doi.org/10.1617/s11527-025-02864-x SN - 1359-5997 VL - 59 IS - 2 SP - 1 EP - 18 PB - Springer Science and Business Media LLC AN - OPUS4-65310 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Stohl, Leonie A1 - Manninger, Tanja A1 - von Werder, Julia A1 - Dehn, F. A1 - Gorbushina, Anna A1 - Meng, Birgit T1 - Bioreceptivity of concrete - A review N2 - Materials that support natural biodiversity on their surfaces can compensate for human activities that have a negative impact on nature and thus contribute to a carbon-neutral and nature-positive world. Specifically designing bioreceptive materials which favor the growth of biofilms on their surface is an approach complementing conventional, macroscopic green façades. But what exactly characterizes a bioreceptive substrate and how do biofilm and substrate interact? How and why does a spontaneous colonization and the formation of biofilms take place? What are biofilms and how can they be established in a laboratory setting? How can this existing knowledge be transferred to the artificial stone concrete so that this material can be tuned to increase (or decrease) its bioreceptivity? This review paper aims at summarizing the existing state of knowledge on bioreceptive concrete and pointing out inconsistencies and contradictions which can only be removed by more interdisciplinary research in the field. KW - Bioreceptivity KW - Biofilm KW - Green facades KW - Developing building materials KW - Surface interactions KW - Concrete PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-581685 DO - https://doi.org/10.1016/j.jobe.2023.107201 SN - 2352-7102 VL - 76 SP - 1 EP - 17 PB - Elsevier AN - OPUS4-58168 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -