TY - JOUR
A1 - Schreiber, Frank
A1 - Zimmermann, M.
A1 - Escrig, S.
A1 - Lavik, G.
A1 - Kuypers, M.M.M.
A1 - Meibom, A.
A1 - Ackermann, M.
T1 - Substrate and electron donor limitation induce phenotypic heterogeneity in different metabolic activities in a green sulphur bacterium
N2 - Populations of genetically identical cells can display marked variation in phenotypic traits; such variation is termed phenotypic heterogeneity. Here, we investigate the effect of substrate and electron donor limitation on phenotypic heterogeneity in N2 and CO2 fixation in the green sulphur bacterium Chlorobium phaeobacteroides. We grew populations in chemostats and batch cultures and used stable isotope labelling combined with nanometer‐scale secondary ion mass spectrometry (NanoSIMS) to quantify phenotypic heterogeneity. Experiments in H2S (i.e. electron donor) limited chemostats show that varying levels of NH4+ limitation induce heterogeneity in N2 fixation. Comparison of phenotypic heterogeneity between chemostats and batch (unlimited for H2S) populations indicates that electron donor limitation drives heterogeneity in N2 and CO2 fixation. Our results demonstrate that phenotypic heterogeneity in a certain metabolic activity can be driven by different modes of limitation and that heterogeneity can emerge in different metabolic processes upon the same mode of limitation. In conclusion, our data suggest that limitation is a general driver of phenotypic heterogeneity in microbial populations.
KW - NanoSIMS
KW - Phenotypic heterogeneity
PY - 2018
UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/1758-2229.12616
DO - https://doi.org/10.1111/1758-2229.12616
SN - 1758-2229
VL - 10
IS - 2
SP - 179
EP - 183
PB - John Wiley & Sons Ltd
AN - OPUS4-44596
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Dombinov, V.
A1 - Herzel, Hannes
A1 - Meiller, M.
A1 - Müller, F.
A1 - Willbold, S.
A1 - Zang, J. W.
A1 - da Fonseca-Zang, W. A.
A1 - Adam, Christian
A1 - Klose, H.
A1 - Poorter, H.
A1 - Jablonowski, N. D.
A1 - Schrey, S. D.
T1 - Sugarcane bagasse ash as fertilizer for soybeans: Effects of added residues on ash composition, mineralogy, phosphorus extractability and plant availability
N2 - Sugarcane bagasse is commonly combusted to generate energy. Unfortunately, recycling strategies rarely consider the resulting ash as a potential fertilizer. To evaluate this recycling strategy for a sustainable circular economy, we characterized bagasse ash as a fertilizer and measured the effects of co-gasification and co-combustion of bagasse with either chicken manure or sewage sludge: on the phosphorus (P) mass fraction, P-extractability, and mineral P phases. Furthermore, we investigated the ashes as fertilizer for soybeans under greenhouse conditions. All methods in combination are reliable indicators helping to assess and predict P availability from ashes to soybeans. The fertilizer efficiency of pure bagasse ash increased with the ash amount supplied to the substrate. Nevertheless, it was not as effective as fertilization with triple-superphosphate and K2SO4, which we attributed to lower P availability. Co-gasification and co-combustion increased the P mass fraction in all bagasse-based ashes, but its extractability and availability to soybeans increased only when co-processed with chicken manure, because it enabled the formation of readily available Ca-alkali phosphates. Therefore, we recommend co-combusting biomass with alkali-rich residues to increase the availability of P from the ash to plants.
KW - Combustion and gasification
KW - Phosphate extractability and availability
KW - X-ray diffraction (XRD)
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-567632
DO - https://doi.org/10.3389/fpls.2022.1041924
SN - 1664-462X
VL - 13
SP - 1
EP - 13
PB - Frontiers Media
CY - Lausanne
AN - OPUS4-56763
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Schoknecht, Ute
A1 - Drescher, P.
A1 - Fischer, M.
A1 - Fürhapper, C.
A1 - Gunschera, J.
A1 - Hill, R.
A1 - Melcher, E.
A1 - Wegner, R.
A1 - Wilken, U.
A1 - Wittenzellner, J.
T1 - Suitability of analytical methods to determine tebuconazole, propiconazole and permethrin in aged wood samples
N2 - The suitability of common analytical methods for the determination of active substances from wood preservatives in aged wood samples was investigated during an interlaboratory study. Permethrin, propiconazole and tebuconazole were quantified in 1.5 and 8 year-old wood samples by gas chromatography and liquid chromatography. Generally, the applied Methods yielded reliable results for these samples. However, wood components can coelute with propiconazole and tebuconazole during liquid chromatography. Optimization of separation might be required if UV detection is applied.
KW - Wood samples
KW - Biocides
KW - Analytical methods
PY - 2020
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-503959
DO - https://doi.org/10.1007/s00107-020-01496-y
VL - 78
IS - 2
SP - 271
EP - 279
PB - Springer
CY - Heidelberg
AN - OPUS4-50395
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - CONF
A1 - Kjaervik, Marit
A1 - Schwibbert, Karin
A1 - Dietrich, P.
A1 - Thissen, A.
A1 - Unger, Wolfgang
T1 - Surface characterisation of bacteria and biofilms by NAP-XPS
N2 - The XPS information depth of approximately 10 nm is in the same size range as the outer membrane of the gram-negative bacteria, which makes XPS a suitable tool for determining the elemental composition of the bacterial surface and monitor changes caused by outer stress like dehydration or exposure to antimicrobials. However, bacteria are inherently in a hydrated state, and therefore only compatible to ultra-high vacuum after extensive sample preparation, which may degrade the sample constituents. This issue is addressed by the development of near-ambient pressure (NAP)-XPS, which enables bacteria and biofilms to be characterised in their native wet state. Artificial biofilms, bacteria and biofilms of Escherichia coli have been characterised with the laboratory NAP-XPS instrument EnviroESCA from SPECS GmbH, at pressures ranging from high vacuum to 12 mbar, and in both humid and dry environment. By studying biological samples in their native wet state, new insight about composition and transport of drugs through cell membranes and the extracellular polymeric substance (EPS) of biofilms can be obtained. In this contribution, the latest progress on biofilm characterisation by NAP-XPS will be presented, and measurement capabilities and limitations will be discussed.
T2 - Die Frühjahrstagung der Deutsche Physikalische Gesellschaft
CY - Berlin, Germany
DA - 12.03.2018
KW - Biofilms
KW - E. coli
KW - Alginate
KW - NAP-XPS
PY - 2018
AN - OPUS4-44500
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - CONF
A1 - Kjaervik, Marit
A1 - Schwibbert, Karin
A1 - Dietrich, P.
A1 - Thissen, A.
A1 - Unger, Wolfgang
T1 - Surface characterisation of biofilms by nearambient pressure X-ray photoelectron spectroscopy
N2 - The XPS information depth of approximately 10 nm is in the same size range as the outer membrane of the gram-negative bacteria, which makes XPS a suitable tool for determining the elemental composition of the bacterial surface and monitor changes caused by outer stress like dehydration or exposure to antimicrobials. However, bacteria are inherently in a hydrated state, and therefore only compatible to ultra-high vacuum after extensive sample preparation, which may degrade the sample constituents. This issue is addressed by the development of near-ambient pressure (NAP)-XPS, which enables bacteria and biofilms to be characterised in their native wet state. Artificial biofilms, bacteria and biofilms of Escherichia coli have been characterised with the laboratory NAP-XPS instrument EnviroESCA from SPECS GmbH, at pressures ranging from high vacuum to 12 mbar, and in both humid and dry environment. By studying biological samples in their native wet state, new insight about composition and transport of drugs through cell membranes and the extracellular polymeric substance (EPS) of biofilms can be obtained.
T2 - Royal Society of Chemistry Twitter Conference
CY - Worldwide (online conference)
DA - 06.03.2018
KW - Biofilms
KW - E. coli
KW - Alginate
KW - NAP-XPS
PY - 2018
AN - OPUS4-44498
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - CONF
A1 - Kjaervik, Marit
A1 - Dietrich, P.
A1 - Thissen, A.
A1 - Schwibbert, Karin
T1 - Surface characterisation of biological samples by near-ambient pressure XPS
N2 - A presentation held for the seminar "Ausgewählte analytische Methoden der Physik" hosted by Prof. Birgit Kanngießer at TU Berlin. The first part focus on depth-dependent XPS-measurements (XPS, synchrotron HAXPES) to obtain a concentration profile of iodine in an artificial biofilm. In the second part, NAP-XPS measurements of various bacterial samples are presented.
T2 - Forschungsseminar "Ausgewählte analytische Methoden der Physik", TU Berlin
CY - Berlin, Germany
DA - 17.07.2018
KW - Biofilms
KW - Alginate
KW - Agarose
KW - HAXPES
KW - NAP-XPS
PY - 2018
AN - OPUS4-45505
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Kjaervik, Marit
A1 - Schwibbert, Karin
A1 - Dietrich, P.
A1 - Thissen, A.
A1 - Unger, Wolfgang
T1 - Surface characterisation of Escherichia coli under various conditions by near-ambient pressure XPS
N2 - Bacteria are inherently in a hydrated state and therefore not compatible to ultra-high vacuum techniques such as XPS without prior sample preparation involving freeze drying or fast freezing.
This has changed with the development of near-ambient pressure (NAP)-XPS, which makes it possible to characterise the bacterial surface with minimal sample preparation. This paper presents NAP-XPS measurements of Escherichia coli under various NAP conditions: at 11 mbar in a humid environment, at 2 mbar after drying in the chamber, pre-dried at 4 mbar, and at 1 mbar after overnight pumping at 10^−4 mbar. The high-resolution spectra of carbon, nitrogen, and oxygen are presented and found to be in general agreement with XPS measurements from freeze-dried and fast-frozen bacteria. However, it was found that the amount of carbon components associated with polysaccharides increases relative to aliphatic carbon during drying and increases further after overnight pumping. This implies that drying has an impact on the bacterial surface.
T2 - European conference on applications of surface and interface analysis (ECASIA'17)
CY - Montpellier, France
DA - 24.09.2018
KW - Bacteria
KW - E. coli
KW - NAP-XPS
PY - 2018
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-454047
DO - https://doi.org/10.1002/sia.6480
SN - 0142-2421
SN - 1096-9918
VL - 50
IS - 11
SP - 996
EP - 1000
PB - John Wiley & Sons
CY - Hoboken, New Jersey, USA
AN - OPUS4-45404
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Söftje, M.
A1 - Weingartz, T.
A1 - Plarre, Rüdiger
A1 - Gjikaj, M.
A1 - Namysko, J. C.
A1 - Kaufmann, D. E.
T1 - Surface Tuning of Wood via Covalent Modification of Its Lignocellulosic Biopolymers with Substituted BenzoatesA Study on Reactivity, Efficiency, and Durability
N2 - Chemical modification of wood applying benzotriazolyl-activated carboxylic acids has proven to be a versatile method for the durable functionalization of its lignocellulosic biopolymers. Through this process, the material properties of Wood can be influenced and specifically optimized. To check the scope and limitations of this modification method, various benzamide derivatives with electron-withdrawing (EWG) or electron-donating (EDG) functional groups in different positions of the aromatic ring were synthesized and applied for covalent modification of Scots pine (Pinus sylvestris L.) sapwood in this study. The bonded amounts of substances (up to 2.20 mmol) were compared with the reactivity constants of the Hammett equation, revealing a significant correlation between the modification efficiency and the theoretical reactivity constants of the corresponding aromatic substitution pattern. The successful covalent attachment of the respective substituted benzamides was proven by attenuated total reflection infrared (ATR-IR) spectroscopy, while the stability of the newly formed ester bond was proven in a standardized leaching test.
KW - Leaching
KW - Wood Protection
KW - Wood Modification
PY - 2021
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-538932
DO - https://doi.org/10.1021/acsomega.1c04353
VL - 6
IS - 49
SP - 33542
EP - 33553
AN - OPUS4-53893
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - CHAP
A1 - Simon, Franz-Georg
A1 - Meggyes, Tamas
ED - Zhang, H. X.
T1 - Sustainable Remediation Methods for Metals and Radionuclides
N2 - Sustainability has become the conscientious and future-oriented principle of modern resource management and environmental protection because caring for the future is tantamount to providing manageable and healthy surroundings for ourselves. For the foreseeable future, geotechnical and environmental engineers must therefore be concerned with ensuring a healthy balance between extraction, processing, manufacturing, utilization, recycling, and disposal of materials and products.
KW - Permeable reactive barriers
KW - Uranium mining
KW - Groundwater
KW - Electrochemical remediation
PY - 2023
SN - 978-1-0716-2465-4
DO - https://doi.org/10.1007/978-1-0716-2466-1_63
SN - 2629-2378
SP - 251
EP - 284
PB - Springer Science+Business Media
CY - Berlin, Heidelberg
ET - 1
AN - OPUS4-58018
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - CHAP
A1 - Simon, Franz-Georg
A1 - Meggyes, Tamas
ED - Meyers, Robert A.
T1 - Sustainable remediation methods for metals and radionuclides
N2 - Since it was realized that sites contaminated with metals and radionuclides needed treatment, various remediation methods have been and are being developed. Depending on the size of the contaminated site and urgency of intervention, conventional or recently introduced techniques have been used. Conventional techniques include excavation and removal for treatment of soil and contaminants, or the so-called “pump-and-treat” method, in which contaminated groundwater is removed from the ground by pumping and treated in a treatment plant on the surface. It has the advantage of using proven techniques and is easy to control, and the treated groundwater can be reinjected into the ground or discharged in rivers or lakes. Novel methods include permeable reactive barriers, biomineralization, and electrokinetic remediation.
KW - Permeable reactive barriers
KW - Groundwater
KW - Uranium
PY - 2019
SN - 978-1-4939-2493-6
DO - https://doi.org/10.1007/978-1-4939-2493-6_63-3
SP - 1
EP - 37
PB - Springer Science+Business Media, LLC
CY - Heidelberg
ET - 1
AN - OPUS4-48816
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Schraut, Katharina
A1 - Adamczyk, Burkart
A1 - Adam, Christian
A1 - Stephan, D.
A1 - Meng, Birgit
A1 - Simon, Sebastian
A1 - von Werder, Julia
T1 - Synthesis and characterisation of alites from reduced basic oxygen furnace slags
N2 - Basic oxygen furnace slags (BOFS) are by-products of the steelmaking process. Several researchers have studied the production of Portland cement clinker and metallic iron from BOFS via a reductive treatment.
In this study, we applied a carbothermal reduction of BOFS in a technical-scale electric arc furnace and characterised the clinker-like products. Those clinker-like non-metallic products (NMPs) had a chemical and mineralogical composition comparable to clinker for ordinary Portland cement (OPC) and contained large elongated alite crystals as major component. The pure NMPs reacted more slowly and achieved a lower degree of hydration compared with commercial OPC.
If the reactivity of the products can be further increased by employing specific adaptations, it can be used as a full clinker substitute for OPC. Nevertheless, it is also an option to use the material without further modifications as a cement component or concrete addition, which contributes to the strength development in both cases.
KW - BOFS
KW - Hydration products
KW - Thermal analysis
KW - X-ray diffraction
PY - 2021
DO - https://doi.org/10.1016/j.cemconres.2021.106518
SN - 0008-8846
VL - 147
SP - 6518
PB - Elsevier Ltd.
AN - OPUS4-52939
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Cros, A.
A1 - Alfaro Espinoza, Gabriela
A1 - De Maria, A.
A1 - Wirth, N. T.
A1 - Nikel, P. I.
T1 - Synthetic metabolism for biohalogenation
N2 - The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of Living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.
KW - Halogenation
KW - Synthetic metabolism
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-542013
DO - https://doi.org/10.1016/j.copbio.2021.11.009
SN - 0958-1669
SN - 1879-0429
VL - 74
SP - 180
EP - 193
PB - Elsevier
CY - Amsterdam
AN - OPUS4-54201
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Tang, Chi-Long
A1 - Seeger, Stefan
T1 - Systematic ranking of filaments regarding their particulate emissions during fused filament fabrication 3D printing by means of a proposed standard test method
N2 - The diversity of fused filament fabrication (FFF) filaments continues to grow rapidly as the popularity of FFF-3D desktop printers for the use as home fabrication devices has been greatly increased in the past decade. Potential harmful emissions and associated health risks when operating indoors have induced many emission studies. However, the lack of standardization of measurements impeded an objectifiable comparison of research findings. Therefore, we designed a chamber-based standard method, i.e., the strand printing method (SPM), which provides a standardized printing procedure and quantifies systematically the particle emission released from individual FFF-3D filaments under controlled conditions. Forty-four marketable filament products were tested. The total number of emitted particles (TP) varied by approximately four orders of magnitude (1E9 ≤ TP ≤ 1E13), indicating that origin of polymers, manufacturer-specific additives, and undeclared impurities have a strong influence. Our results suggest that TP characterizes an individual filament product and particle emissions cannot be categorized by the polymer type (e.g., PLA or ABS) alone. The user's choice of a filament product is therefore decisive for the exposure to released particles during operation. Thus, choosing a filament product awarded for low emissions seems to be an easily achievable preemptive measure to prevent health hazards.
KW - Emission test method
KW - FFF-printing
KW - Particle emission
KW - Indoor air quality
KW - FFF-filament
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-545087
DO - https://doi.org/https://doi.org/10.1111/ina.13010
SN - 1600-0668
VL - 32
IS - 3
SP - 1
EP - 12
PB - Wiley
CY - Hoboken, New Jersey, USA
AN - OPUS4-54508
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Neuhold, S.
A1 - Algermissen, D.
A1 - Drissen, P.
A1 - Adamczyk, Burkart
A1 - Presoly, P.
A1 - Sedlazeck, K. P.
A1 - Schenk, J.
A1 - Raith, J. G.
A1 - Pomberger, R.
A1 - Vollprecht, D.
T1 - Tailoring the FeO/SiO2 Ratio in Electric Arc Furnace Slags to Minimize the Leaching of Vanadium and Chromium
N2 - Based on recently published research on leaching control mechanisms in electric arc furnace (EAF) slags, it is assumed that a FeO/SiO2 ratio of around one leads to low leached V and Cr concentrations. This ratio influences the mineral phase composition of the slag toward higher amounts of spinel and a lower solubility of calcium silicate phases by suppressing the formation of magnesiowuestite and highly soluble calcium silicate phases. To evaluate this hypothesis, laboratory and scaled up tests in an EAF pilot plant were performed on slag samples characterized by elevated V and Cr leaching and a high FeO/SiO2 ratio. Prior to the melting experiments, the optimum FeO/SiO2 ratio was calculated via FactSageTM. In the melting experiments, the ratio was adjusted by adding quartz sand, which also decreased the basicity (CaO/SiO2) of the slag. As a reference, remelting experiments without quartz sand addition were conducted and additionally, the influence of the cooling rate of the slag was examined. The remelted (without quartz sand) and the remelted modified slags (with quartz sand) were analyzed chemically and mineralogically and the leaching behavior was investigated. The modification of the slags yielded a minimized release of V and Cr, supporting the hypothesis that the FeO/SiO2 ratio influences the mineralogy and the leaching behavior.
KW - Electric arc furnace (EAF) slags
KW - FactSageTM calculations
KW - Leaching control mechanisms
KW - Melting experiments
PY - 2020
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-571517
DO - https://doi.org/10.3390/app10072549
VL - 10
IS - 7
SP - 1
EP - 18
PB - MDPI
AN - OPUS4-57151
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Adamczyk, Burkart
A1 - Neuhold, S.
A1 - Drissen, P.
A1 - Algermissen, D.
A1 - Presoly, P.
A1 - Sedlazeck, K. P.
A1 - Schenk, J.
A1 - Raith, J. G.
A1 - Pomberger, R.
A1 - Vollprecht, D.
T1 - Tailoring the FeO/SiO2 ratio in electric arc furnace slags to minimize the leaching of Vanadium and Chromium
N2 - Based on recently published research on leaching control mechanisms in electric arc furnace (EAF) slags, it is assumed that a FeO/SiO2 ratio of around one leads to low leached V and Cr concentrations. This ratio influences the mineral phase composition of the slag toward higher amounts of spinel and a lower solubility of calcium silicate phases by suppressing the formation of magnesiowuestite and highly soluble calcium silicate phases. To evaluate this hypothesis, laboratory and scaled up tests in an EAF pilot plant were performed on slag samples characterized by elevated V and Cr leaching and a high FeO/SiO2 ratio. Prior to the melting experiments, the optimum FeO/SiO2 ratio was calculated via FactSageTM. In the melting experiments, the ratio was adjusted by adding quartz sand, which also decreased the basicity (CaO/SiO2) of the slag. As a reference, remelting experiments without quartz sand addition were conducted and additionally, the influence of the cooling rate of the slag was examined. The remelted (without quartz sand) and the remelted modified slags (with quartz sand) were analyzed chemically and mineralogically and the leaching behavior was investigated. The modification of the slags yielded a minimized release of V and Cr, supporting the hypothesis that the FeO/SiO2 ratio influences the mineralogy and the leaching behavior.
KW - Electric arc furnace slags
KW - FactSage calculations
KW - EAF slags
KW - Leaching control mechanisms
KW - Melting experiments
PY - 2020
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-507748
DO - https://doi.org/10.3390/app10072549
VL - 10
IS - 7
SP - 2549
EP - 2565
AN - OPUS4-50774
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Roesch, Philipp
A1 - Vogel, Christian
A1 - Wittwer, Philipp
A1 - Huthwelker, T.
A1 - Borca, C.
A1 - Sommerfeld, Thomas
A1 - Kluge, Stephanie
A1 - Piechotta, Christian
A1 - Kalbe, Ute
A1 - Simon, Franz-Georg
T1 - Taking a Look at the Surface: µ-XRF Mapping and Fluorine K-edge µ-XANES Spectroscopy of Organofluorinated Compounds in Environmental Samples and Consumer Products
N2 - For the first time, µ-X-ray fluorescence (µ-XRF) mapping combined with fluorine K-edge µ-X-ray absorption near-edge structure (µ-XANES) spectroscopy was applied to depict per- and polyfluoroalkyl substances (PFAS) contamination and inorganic fluoride in samples concentrations down to 100 µg/kg fluoride. To demonstrate the matrix tolerance of the method, several PFAS contaminated soil and sludge samples as well as selected consumer product samples (textiles, food contact paper and permanent baking sheet) were investigated. µ-XRF mapping allows for a unique element-specific visualisation at the sample surface and enables localisation of fluorine containing compounds to a depth of 1 µm. Manually selected fluorine rich spots were subsequently analysed via fluorine K-edge µ-XANES spectroscopy. To support spectral interpretation with respect to inorganic and organic chemical distribution and compound class determination, linear combination (LC) fitting was applied to all recorded µ-XANES spectra. Complementarily, solvent extracts of all samples were target-analysed via LC-MS/MS spectrometry. The detected PFAS sum values range from 20 to 1136 µg/kg dry weight (dw). All environmentally exposed samples revealed higher concentration of PFAS with a chain length >C8 (e.g. 580 µg/kg dw PFOS for Soil1), whereas the consumer product samples showed a more uniform distribution with regard to chain lengths from C4 to C8. Independent from quantified PFAS amounts via target analysis, µ-XRF mapping combined with µ-XANES spectroscopy was successfully applied to detect both point-specific concentration maxima and evenly distributed surface coatings of fluorinated organic contaminants in the corresponding samples.
KW - PFAS
KW - XRF
KW - LC-MS/MS
KW - XANES
KW - Fluoride
KW - Soil
PY - 2023
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-576109
DO - https://doi.org/10.1039/D3EM00107E
SN - 2050-7887
SP - 1
EP - 12
PB - Royal Society of Chemistry
AN - OPUS4-57610
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - CONF
A1 - Vandrich, Jasmina
A1 - Alfaro Espinoza, Gabriela
A1 - Kunte, Hans-Jörg
T1 - Tampering with ectoine production and excretion in Halomonas elongata using CRISPRi and gene knock-outs
N2 - The halophilic bacterium Halomonas elongata can tolerate salt concentrations above 10% NaCl and uses the accumulation of the compatible solute ectoine as a major osmoregulatory mechanism. Ectoine can be accumalted inside the cell through import from the medium or de novo synthesis and establishes an osmotic equilibrium with the surrounding1. Ectoine also protects proteins from the effects of freezing, drying and high temperatures4 and DNA from ionizing radiation2. These features make ectoine a valuable compound for cosmetics and medical devices.
H. elongata was originally isolated from a solar salt facility, where it thrives under high salt concentrations. It was found that marine prokaryotes, which are exposed to high oxidative stress in their environment, vary glycolytic strategies5. A variation in the use of the glucose metabolic pathways is also assumed for H. elongata.
T2 - International Society for Microbial Ecology
CY - Leipzig, Germany
DA - 12.08.2018
KW - Halomonas CRISPR Ectoine
PY - 2018
AN - OPUS4-46080
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Nilsson, R. H.
A1 - Taylor, A. F. S.
A1 - Adams, R. I.
A1 - Baschien, C.
A1 - Bengtsson-Palme, J.
A1 - Cangren, P.
A1 - Coleine, C.
A1 - Iršėnaitė, R.
A1 - Martin-Sanchez, Pedro Maria
A1 - Meyer, W.
A1 - Oh, S.-Y.
A1 - Sampaio, J. P.
A1 - Seifert, K. A.
A1 - Sklenář, F.
A1 - Stubbe, D.
A1 - Suh, S.-O.
A1 - Summerbell, R.
A1 - Svantesson, S.
A1 - Unterseher, M.
A1 - Visagie, C. M.
A1 - Weiss, M.
A1 - Woudenberg, J. HC.
A1 - Wurzbacher, C.
A1 - Van den Wyngaert, S.
A1 - Yilmaz, N.
A1 - Yurkov, A.
A1 - Kõljalg, U.
A1 - Abarenkov, K.
A1 - Daniel, H.-M.
A1 - Glassman, S. I.
A1 - Hirooka, H.
A1 - Irinyi, L.
T1 - Taxonomic annotation of public fungal ITS sequences from the built environment – a report from an April 10–11, 2017 workshop (Aberdeen, UK)
N2 - Recent DNA-based studies have shown that the built environment is surprisingly rich in fungi. These indoor fungi – whether transient visitors or more persistent residents – may hold clues to the rising levels of human allergies and other medical and building-related health problems observed globally. The taxo¬nomic identity of these fungi is crucial in such pursuits. Molecular identification of the built mycobiome is no trivial undertaking, however, given the large number of unidentified, misidentified, and technically compromised fungal sequences in public sequence databases. In addition, the sequence metadata required to make informed taxonomic decisions – such as country and host/substrate of collection – are often lacking even from reference and ex-type sequences. Here we report on a taxonomic annotation workshop (April 10–11, 2017) organized at the James Hutton Institute/University of Aberdeen (UK) to facilitate reproducible studies of the built mycobiome. The 32 participants went through public fungal ITS bar¬code sequences related to the built mycobiome for taxonomic and nomenclatural correctness, technical quality, and metadata availability. A total of 19,508 changes – including 4,783 name changes, 14,121 metadata annotations, and the removal of 99 technically compromised sequences – were implemented in the UNITE database for molecular identification of fungi (https://unite.ut.ee/) and shared with a range of other databases and downstream resources. Among the genera that saw the largest number of changes were Penicillium, Talaromyces, Cladosporium, Acremonium, and Alternaria, all of them of significant importance in both culture-based and culture-independent surveys of the built environment.
KW - Indoor mycobiome
KW - Built environment
KW - Molecular identification
KW - Fungi
KW - Taxonomy
KW - Systematics
KW - Sequence annotation
KW - Metadata
KW - Open data
PY - 2018
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-438949
DO - https://doi.org/10.3897/mycokeys.28.20887
SN - 1314-4049
SN - 1314-4057
VL - 28
SP - 65
EP - 82
PB - Pensoft Publishers
CY - Washington, DC
AN - OPUS4-43894
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Usmani, Shirin M.
A1 - Plarre, Rüdiger
A1 - Hübert, Thomas
A1 - Kemnitz, E.
T1 - Termite resistance of pine wood treated with nano metal fluorides
N2 - Fluorides are well-known as wood preservatives. One of the limitations of fluoride-based wood preservatives is their high leachability. Alternative to current fluoride salts such as NaF used in wood protection are low water-soluble fluorides. However, impregnation of low water-soluble fluorides into wood poses a challenge. To address this challenge, low water-soluble fluorides like calcium fluoride (CaF2) and magnesium fluoride (MgF2) were synthesized as nanoparticles via the fluorolytic sol−gel synthesis and then impregnated into wood specimens. In this study, the toxicity of nano metal fluorides was assessed by termite mortality, mass loss and visual analysis of treated specimens after eight weeks of exposure to termites, Coptotermes formosanus. Nano metal fluorides with sol concentrations of 0.5 M and higher were found to be effective against termites resulting in 100% termite mortality and significantly inhibited termite feeding. Among the formulations tested, the least damage was found for specimens treated with combinations of CaF2 and MgF2 with an average mass loss less than 1% and visual rating of “1”. These results demonstrate the efficacy of low water-soluble nano metal fluorides to protect Wood from termite attack.
KW - Wood Protection
KW - Nano Particles
KW - Termites
PY - 2020
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-508377
DO - https://doi.org/10.1007/s00107-020-01522-z
VL - 78
SP - 493
EP - 499
PB - Springer
AN - OPUS4-50837
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Usmani, Shirin M.
A1 - Plarre, Rüdiger
A1 - Hübert, Thomas
A1 - Kemnitz, E.
ED - Richter, K.
ED - Van de Kulien, J.-W.
T1 - Termite resistance of pine wood treated with nano metal fluorides
N2 - Fluorides are well-known as wood preservatives. One of the limitations of fluoride-based wood preservatives is their high leachability. Alternative to current fluoride salts such as NaF used in wood protection are low water-soluble fluorides. However, impregnation of low water-soluble fluorides into wood poses a challenge. To address this challenge, low water-soluble fluorides like calcium fluoride (CaF2) and magnesium fluoride (MgF2) were synthesized as nanoparticles via the fluorolytic sol−gel synthesis and then impregnated into wood specimens. In this study, the toxicity of nano metal fluorides was assessed by termite mortality, mass loss and visual analysis of treated specimens after eight weeks of exposure to termites, Coptotermes formosanus. Nano metal fluorides with sol concentrations of 0.5 M and higher were found to be effective against termites resulting in 100% termite mortality and significantly inhibited termite feeding. Among the formulations tested, the least damage was found for specimens treated with combinations of CaF2 and MgF2 with an average mass loss less than 1% and visual rating of “1”. These results demonstrate the efficacy of low water-soluble nano metal fluorides to protect wood from termite attack.
KW - Holzschutzmittel
KW - Nanoparticles
KW - Fluorides
KW - Termites
PY - 2020
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-514325
DO - https://doi.org/10.1007/s00107-020-01522-z
VL - 78
SP - 493
EP - 499
PB - Springer
CY - Berlin
AN - OPUS4-51432
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -