TY - CONF A1 - Zubia Aranburu, Judith A1 - Cappella, Brunero A1 - Zabala Eguren, A. A1 - Buruaga Lamarain, L. A1 - Aginagalde Lopez, A. A1 - Bonse, Jörn A1 - Schwibbert, Karin T1 - Quantification of the adhesion force of E. coli on Ti via single-cell force spectroscopy N2 - Antibiotic resistance is a growing global problem which poses a massive threat to human health. Although human activity contributes to the acceleration of the process, bacteria have a self-driven stabilisation mechanism to protect themselves from such and other external threats: biofilm formation. Nonetheless, it is the adhesion of a single bacterial cell to a surface that triggers the formation of such network of biomolecules and microorganisms, as well as its hazardous consequences. The main objective of this work was to quantify the adhesion force of a single E. coli cell on a Ti substrate via the AFM-related single-cell force spectroscopy, with both the cell and the substrate material being of high clinical relevance. A set of 25 x 25 force displacement curves was acquired with a maximum force of 3.2 nN without dwell time, yielding a topography map and an adhesion force map that showed to be correlated. A mean adhesion force of 0.85 ± 0.175 nN was measured and the presence of cell appendages on the bacterial cell wall was verified through individual force-displacement curves. Bacterial viability was assessed after the measurements via live/dead staining. T2 - XL Congreso Anual de la Sociedad Española de Ingeniería Biomédica CASEIB 2022 CY - Valladolid, Spain DA - 23.11.2022 KW - Bacteria KW - Atomic force microscopy KW - Force distance curve PY - 2022 SN - 978-84-09-45972-8 SP - 217 EP - 220 AN - OPUS4-57039 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - von Laar, C. A1 - Baar, C. A1 - Plarre, Rüdiger A1 - McMahon, Dino Peter T1 - Genetic relationships of local infestations by Anobium punctatum, Xestobium rufovillosum and their associated predator Korynetes caeruleus from buildings in North-Eastern Germany N2 - Wood-destroying pests such as Anobium punctatum and Xestobium rufovillosum cause damage to art and cultural objects as well as to buildings. Monitoring population dynamics of pest species as well as of their naturally occurring counterparts are an essential part in the development of biological control measures as alternatives to conventional wood protection. Therefore, both the dispersal and homogeneity of pest and beneficial insect populations across multiple sites and buildings were investigated in the present study using DNA barcoding. Specifically, beetles of Anobium punctatum (de Geer 1774) (Coleoptera, Ptinidae), Xestobium rufovillosum (de Geer, 1974) (Coloeptera, Ptinidae), and Korynetes caeruleus (de Geer 1775) (Coleoptera, Cleridae) were collected from buildings at four different sites in Mecklenburg-Western Pomerania, North-Eastern Germany. DNA analysis was performed using mitochondrial cytochrome c oxidase subunit I (COI). For A. punctatum, low base pair variability was found in the gene segment studied (4-5 SNPs) within one building (Greven) and between four spatially separated sites. Conversely, in X. rufovillosum, the sequences from two sites studied were homogeneous within a site but differed between locations by nine base pair positions (SNPs). The main result of this study is that the pests A. punctatum and X. rufovillosum showed a higher variability in the investigated gene segment than the natural counterpart K. caeruleus. T2 - IRG52 Webinar on Wood Protection CY - Online meeting DA - 01.11.21 KW - Anobium punctatum KW - Xestobium rufovillosum KW - Korynetes caeruleus KW - DNA barcode PY - 2021 SN - 2000-8953 VL - 21 SP - 1 EP - 11 PB - The International Research Group on Wood Protection CY - Stockholm, Sweden AN - OPUS4-54197 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Plarre, Rüdiger A1 - Cabral, U. A1 - Querner, P. ED - Ryder, S. ED - Crossman, A. T1 - Screening of two plant-derived extracts from Sri Lanka for their potential to control the subterranean termite Coptotermes formosanus N2 - The tropical environment of Sri Lanka accelerates bio-deterioration of cultural objects. Termites are one of the most damaging insect pests destroying the cellulose components of historical artifacts. Herbal extracts obtained from resin of Vateria copallifera (Retzius) Alston and seeds of Madhuca longifolia (Konig) Macbride have been used for centuries to preserve e.g. palm leaf manuscripts from insect attack. Herbal extractions of these traditional products for palm leaf manuscript were tested for their effect against the termite species Coptotermes formosanus Shiraki, 1909. Natural and artificial aged herbal extractions were tested to obtain a repellent index. Resin oil of V. copallifera caused slightly higher repellencies than M. longifolia. Artificially aged samples produced lower repellencies than naturally aged samples. The results indicate that the active ingredients are volatile. The potential for barrier treatment was tested only with V. copallifera. Tunnelling-behavior of C. formosanus workers through sand in the presence of V. copallifera resin oil was largely reduced. T2 - Pest Odyssey The Next Generation CY - Online meeting DA - 20.09.2021 KW - IPM in museums KW - Termite control KW - Essential oils KW - Library pests PY - 2022 SN - 978-1-909492-83-7 SP - 56 EP - 62 PB - Archetyp Publication LTD CY - London AN - OPUS4-54736 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Plarre, Rüdiger A1 - Busweiler, Sabine A1 - Haustein, V A1 - von Laar, C. A1 - Haustein, T ED - Bueno-Mari, R ED - Montalvo, T ED - Robinson, W H T1 - Korynetes caeruleus (coleoptera: cleridae) for biological control of anobium punctatum (coleoptera, ptinidae) N2 - Larvae and adults of Korynetes caeruleus (de Geer 1775) (Coleoptera: Cleridae) were collected from old churches and reared in the laboratory on Anobium punctatum (de Geer 1774) (Coleoptera, Ptinidea, formerly Anobiidae). Breeding success of K. caeruleus was low, but basic parameters of this species’ developmental cycle were identified. At 21 °C and 75 % relative humidity and a four-month cold period at 4 °C, the development of K. caeruleus from egg to adult appearance lasted 2 years. The pupal stage may be reached and completed after one and a half years. Feeding on larvae of A. punctatum by larvae of K. caeruleus was observed and consisted of a combination of sucking haemolymph and consuming body parts. The sickle-like mandibles of larvae of K. caeruleus penetrate the cuticle of prey larvae followed by pumping and sucking body movements. Adult beetles of A. punctatum were not attacked by K. caeruleus larvae. Feeding behavior of adult K. caeruleus was not investigated. T2 - 10th International Conference on Urban Pests CY - Barcelona, Spain DA - 26.06.2022 KW - Cultural heritage KW - Biological pest control KW - Life history data KW - Wood protection PY - 2022 SN - 978-84-09-41424-6 SP - 34 EP - 44 AN - OPUS4-55170 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Plarre, Rüdiger ED - Trematerra, P. ED - Conti, B. T1 - Uninvited and welcomed guests in museums – biological management of clothes moths and woodworms N2 - In museums and historic houses clothes, moths (Tineola bisselliella, Tinea pellionella) and the furniture beetle (Anobium punctatum) are the most economically important pests on textiles or wooden artifacts, respectively. Their management is essential to protect cultural ethnological heritage and natural history collections for future generations. Pest management strategies have changed over time. Today, intensive knowledge on pest biology and overall material science are key cornerstones in IPM concepts - also for the Museum environment. The important first steps for sustainable pest management are risk assessment, early pest detection and identification of pathways of infestation. These steps are followed by physical and biological means of control, which have lately gained more importance than applying biocides. Several potentially effective biological enemies of clothes moths and woodworm have been known for a long time, but their promotion for pest control in Museums and historic houses is just beginning. This short review summarizes current concepts of pest life cycle interruption by applying good quarantine and very specific biological measures. The lessons learned from recent faunistic surveys, life-history studies as well as behavioral observations of parasitoids and predators of clothes moths and woodworm may supplement the pest management tool box. The need for further research in this field is addressed. T2 - Working Group Meeting "Integrated Protection of Stored Products" CY - Pisa, Italy DA - 03.09.2019 KW - Corynetes caeruleus KW - Biological control KW - Museum pests KW - Tineola bisselliella KW - Anobium punctatum KW - Apanteles carpatus KW - Baryscapus tineivorus PY - 2020 SN - 978-92-9067-333-0 VL - 148 SP - 266 EP - 273 AN - OPUS4-50543 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Piechotta, Christian A1 - Iznaguen, Hassan A1 - Traub, Heike A1 - Feldmann, Ines A1 - Köppen, Robert A1 - Witt, Angelika A1 - Jung, Christian A1 - Becker, Roland A1 - Oleszak, Katrin A1 - Bücker, Michael A1 - Urban, Klaus A1 - Reger, Christian A1 - Ostermann, Markus T1 - Bewitterungsszenarien im Vergleich – Veränderungen in der Oberflächenmorphologie von Polypropylen (PP) und Polystyrol (PS) unter dem Aspekt des Austrags von polybromierten Flammschutzmitteln N2 - Gegenstand der vorzustellenden Arbeiten ist die Prüfung der Umwelt-beständigkeit und -verträglichkeit von Materialien und Produkten hinsichtlich der Emission von potenziellen Schadstoffen in die Umwelt. Hierzu werden chemisch-physikalische Einflüsse (Bewitterung) und mikrobielle Beanspruchungen an Modellmaterialien evaluiert. So werden die Freisetzungsraten von Schadstoffen in Abhängigkeit der Beanspruchung beschrieben. Als Modellmaterialien kommen die Polymere Polystyrol (PS) und Polypropylen (PP) zum Einsatz. Synergistische Effekte der Bewitterungsparameter und der mikrobiologischen Beanspruchung sollen dabei ebenso betrachtet werden, wie die gezielte Alterung. Auch findet eine Beschreibung des Verhaltens der ausgetragenen Schadstoffe in den Umweltkompartimenten Boden oder Wasser statt. Hier sind mit Hilfe der zu entwickelnden Screening- und non-Target-Analyseverfahren die Transformation und der Metabolismus durch Mikroorganismen zu beschreiben. Aus den Ergebnissen sollen Korrelationen zwischen den künstlichen Alterungsverfahren und realen Szenarien abgeleitet werden. T2 - 47. Jahrestagung der GUS CY - Blankenloch-Stutensee, Germany DA - 21.03.2018 KW - Schadstoffaustrag KW - Umweltsimulation KW - Bewitterung PY - 2018 SN - 978-981-18507-2-7 VL - 47 SP - 115 EP - 128 AN - OPUS4-49802 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Palanti, S. A1 - Cragg, S. A1 - Plarre, Rüdiger T1 - Resistance against marine borers: About the revision of EN 275 and the attempt for a new laboratory standard for Limnoria N2 - Wood protection technology in the marine environment has changed over the last decades and will continue to do so. New active ingredients, newer formulations, and novel wood-based materials including physically- and chemically-modified wood, together with increasing concerns over environmental impacts of wood preservatives, urgently demand a major revision of EN 275 “Wood preservatives – Determination of the Protective Effectiveness against Marine Borers”, dated from 1992. This IRG document reports on the technical work in CEN TC 38 regarding the revision of this standard. A Task Group within WG 24 of CEN TC 38 was formed consisting of experts from different field of competence (e.g. wood preservatives industry, wood scientists, marine biologists, archaeologists and cultural heritage conservators). Starting by e-mail correspondence in 2014, and continuing with four physical meetings (Berlin 2x, Florence, Venice) with experts from Germany, Italy, Sweden, and UK were held so far. Significant items for revision in EN 275 were identified as: number of replicates, duration of the test, dimension of specimens, number of test sites, number of reference species, reference material including reference preservative, re-immersion of specimens after non-destructive periodical evaluation for longer periods of time vs higher number of replicates for successive destructive examinations without re-immersion, utilization of X- ray apparatus and specific software to ease evaluation, etc. Furthermore, the task group is working on a standardized lab test for time-saving evaluation of different wood qualities for their potential to resist attack by limnorids. The suitability of this lab test will be determined by round robin tests as soon as safe face-to-face collaboration permits. The outcome will be published as a CEN TR (Technical Report) document, with a view to eventual adoption within the revised standard. T2 - THE INTERNATIONAL RESEARCH GROUP ON WOOD PROTECTION CY - Online meeting DA - 10.06.2020 KW - Standardization KW - Marine borers KW - Bankia sp KW - Teredo sp KW - Limnoria spp KW - Durability PY - 2020 VL - 2020 SP - IRG/WP 20-20669-1 EP - IRG/WP 20-20669-11 CY - Stockholm AN - OPUS4-52064 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Landsberger, B A1 - Frauendorf, H A1 - Adler, C A1 - Plarre, Rüdiger ED - Nilsen, L ED - Rossipal, M T1 - Capability and Limitations of Anoxic Treatments for Protecting Museum Collections N2 - Without precaution, insects may cause serious damage to museum collections. Quarantine of potentially infested objects can be logistically challenging. Anoxia under controlled Nitrogen atmosphere is a most gentle but also time-consuming method to eradicate insect pests in all kinds of different materials. Treatment results are usually affected by duration, temperature, humidity and residual oxygen content. During a two-year research project, 34 relevant pest insect species of all developmental stages were tested in different materials (wood, paper, wool) to monitor treatment success and to determine optimum treatment parameters. Duration of treatment ranged from one to three weeks at temperatures of 20–27 °C. As expected, results showed significant differences in mortality among tested species. Highest tolerance of hypoxic conditions was found in elder larvae of Hylotrupes bajulus. Although this species is an unlikely museum pest, it may serve as an overall most tolerant reference. Anobiids and other wood boring beetles are more often an issue related to cultural heritage. A combination of three weeks exposure time at maximum 0.5% residual oxygen and 24 °C, alternatively 1% residual oxygen and 27 °C are recommended for infested artefacts. Imbedding materials in general had no influence on mortality. This study was funded by Deutsche Bundesstiftung Umwelt (DBU). T2 - 4th International Conference Integrated Pest Management (IPM) for Cultural Heritage CY - Stockholm, Sweden DA - 21.05.2019 KW - Controlled atmosphere KW - Museum insect pest KW - Anoxia KW - Nitrogen PY - 2019 SN - 978-91-7209-845-9 SP - 202 EP - 210 AN - OPUS4-50349 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koerdt, Andrea A1 - An, Biwen Annie A1 - Deland, Eric ED - Eibergen, N. ED - Poulassichidis, T. T1 - Novel Multiport Flow-Column Corrosion Monitoring System (MFC) Revealed High Corrosion Rates by Corrosive Methanogenic Archaea N2 - MFC was used to study the corrosiveness of iron-utilizing methanogen, Methanobacterium IM1 under flow conditions. Comparing against electrical SRM, Desulfovibrio ferrophilus IS5, results showed under standard mesophilic conditions, average corrosion rates of Methanobacterium IM1 was double that of SRM. The highest corrosion rate of Methanobacterium IM1 reached up to 0.60 mm/yr under neutral conditions, and severe pitting was observed on the iron surface. Furthermore, the corrosion products of Methanobacterium IM1 were characterized with TOF-SIM, FIB-SEM and EDX, and preliminary results revealed FeCO3 is not the only corrosion product of Mi-MIC, as previously reported. Under low pH conditions, the maximum corrosion rate of Methanobacterium IM1 reached 1.57 mm/yr, which resulted in severed deformity of the iron specimen. Additional comparisons using different types of incubation material were conducted to standardize MFC MIC testing. T2 - Corrosion 2021 CY - Online Meeting DA - 19.04.2021 KW - MIC KW - Microbiologically influenced corrosion KW - Biocorrosion KW - Hi-Tension KW - Environmental condition KW - Flow Model KW - Modelling KW - Korrosion PY - 2021 UR - https://my.nace.org/PaperTrail/Authors/Submission.aspx?id=2b6387be-7390-ea11-813a-005056a95a7c SP - Paper C2021-16303, 1 AN - OPUS4-52480 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Geburtig, Anja A1 - Gorbushina, Anna A1 - Plarre, Rüdiger A1 - Stephan, Ina T1 - Umweltsimulation an der BAM – Grundlegende Ansätze mit Beispielen aus der natürlichen Umwelt N2 - Drei grundlegend verschiedene Ansätze für Umweltsimulation werden an Beispielen illustriert: (i) Ganzheitlicher Ansatz - Nachstellen von Umweltmilieus im Labor Ziel ist hier das Nachstellen von (kombinierten) Umweltbedingungen im Labor; die Umweltparameter werden mit all ihren Wechselwirkungen aufgebracht. Hauptnutzen ist eine gegenüber der natürlichen Beanspruchung erhöhte Reproduzierbarkeit der Umweltbedingungen. Hat man sein Laborsetup entwickelt, ist es auf verschiedene Materialien anwendbar. Unter solchen Laborbedingungen ermittelte Lebensdauern sind dabei nicht auf die typischerweise sehr variablen Real-Umweltbedingungen übertragbar. (ii) Parametrisierter Ansatz - Ermittlung einzelner Materialempfindlichkeiten Hierbei werden im Labor die Wirkungen separater Umweltparameter auf Materialien nachgestellt. Für eine solche Separation der Einflussfaktoren ist insbesondere die Aufschlüsselung möglicher Wechselwirkungen der Umwelt-parameter (z.B. Mikroklima an bestrahlten Oberflächen) erforderlich. Einzelne (meist Alterungs-) Empfindlichkeiten können qualitativ nachgewiesen werden oder sogar – als Beanspruchungs-Wirkungs-Funktionen – quantifiziert werden, was einen wesentlichen Schritt in Richtung der Digitalisierung der Material¬prüfung darstellt. Insbesondere ist dann auch eine Lebensdauer-vorhersage für vorgegebene Zeitreihen der Beanspruchungs¬parameter umsetzbar. (iii) Rückwirkungen auf die Umwelt Umweltbeanspruchungen können zur Freisetzung von Schadstoffen in die Umwelt führen. Durch die Nachstellung kritischer, aber realitätsnaher Einsatzszenarien kann die Menge an freigesetzten Substanzen abgeschätzt werden. Egal, welcher Ansatz verfolgt wird – ein Vergleich mit der oder einer Real-beanspruchung ist unerlässlich, ebenso wie die Messdatenaufzeichnung (data logging) aller potenziell relevanten Beanspruchungsparameter während dieser Realbeanspruchung. Obwohl die naturnahe Umwelt – sowohl in der BAM als auch bei der GUS – gegenüber der technischen Umwelt eher untergeordnet auftritt, werden zur Illustration Beispiele aus der naturnahen Umwelt verwendet. T2 - 50. Jahrestagung der GUS CY - Online meeting DA - 23.03.2022 KW - Umweltsimulation PY - 2022 SN - 978-3-9818507-7-2 SP - 79 EP - 89 AN - OPUS4-55015 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Kunte, Hans-Jörg A1 - Koerdt, Andrea ED - Eibergen, N. ED - Poulassichidis, T. T1 - Microbiologically Influenced Corrosion (MIC) by Halophilic (Salt-Loving) Nitrate and Sulfate-Reducing Microorganisms N2 - The survey of Canadian shale sites showed a dominance of halophilic microorganisms, including Halomonas (HA). Nitrate-amended incubations of the field samples under high salinity (14.6% NaCl), revealed a dominance of HA (>72%) and an accumulation of nitrite. Nitrite accumulation directly inhibited the growth of SRB, thereby decreasing their souring and corrosion risks. However, accumulated nitrite may also contribute to iron corrosion, which will be tested by using different concentrations of nitrate as an electron acceptor to HA. Different salinities are further tested on HA strains supplemented with iron coupons to determine their effects on iron corrosion rates. HA incubated with separate cultures of corrosive methanogen and SRB were tested to determine whether a positive or adverse effect will occur between them. Lastly, analyses of iron coupons will be conducted using TOF-SIMS, FIB-SEM and EDS for corrosion product characterization T2 - Corrosion 2021 CY - Online Meeting DA - 19.04.2021 KW - MIC KW - Bacteria KW - Halophile KW - Corrosion KW - Environmental condition KW - Korrosion KW - High salinity PY - 2021 UR - https://my.nace.org/PaperTrail/Authors/Submission.aspx?id=2914f145-7f8f-ea11-813a-005056a95a7c SP - Paper C2021-16284, 1 AN - OPUS4-52479 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -