TY - CONF A1 - Jaut, Valerie T1 - Antibiotic tolerance of biofilms emerging from multicellular effects of antibiotic efflux N2 - Biofilms are multicellular assemblies of bacteria living in a self-produced extracellular matrix. One characteristic of biofilms is that they are difficult to kill. Different mechanisms, like the development of persister cells or efflux pumps which pump some antimicrobials out of the cell, make them tolerant. Our central hypothesis is that efflux pump activity causes emergent antimicrobial tolerance of multicellular bacterial populations, through the interplay of efflux-mediated spatial interactions and efflux-linked persistence. To verify the hypothesis, we combine computational modelling with information gained from 3 types of multicellular assemblies. We are currently generating strains that differ in their levels of efflux activity, mixes are then cultivated together in the 3 model systems. In colonies the link between structure and spatial patterns of gene expression will be characterized. Using a microfluidic device, the interactions range of efflux as a response to different antimicrobials will be determined. In a flow chamber a 3D biofilm will be generated, to investigate the biofilm development over time and persister cell formation. All results will be compared with model predictions. T2 - UNA Workshop CY - Berlin, Germany DA - 29.01.2024 KW - Antibiotic KW - Biofilm KW - Tolerance KW - Efflux PY - 2024 AN - OPUS4-61280 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Geburtig, Anja A1 - Gorbushina, Anna A1 - Plarre, Rüdiger A1 - Stephan, Ina T1 - Umweltsimulation an der BAM – Grundlegende Ansätze mit Beispielen aus der natürlichen Umwelt N2 - Drei grundlegend verschiedene Ansätze für Umweltsimulation werden an Beispielen illustriert: (i) Ganzheitlicher Ansatz - Nachstellen von Umweltmilieus im Labor Ziel ist hier das Nachstellen von (kombinierten) Umweltbedingungen im Labor; die Umweltparameter werden mit all ihren Wechselwirkungen aufgebracht. Hauptnutzen ist eine gegenüber der natürlichen Beanspruchung erhöhte Reproduzierbarkeit der Umweltbedingungen. Hat man sein Laborsetup entwickelt, ist es auf verschiedene Materialien anwendbar. Unter solchen Laborbedingungen ermittelte Lebensdauern sind dabei nicht auf die typischerweise sehr variablen Real-Umweltbedingungen übertragbar. (ii) Parametrisierter Ansatz - Ermittlung einzelner Materialempfindlichkeiten Hierbei werden im Labor die Wirkungen separater Umweltparameter auf Materialien nachgestellt. Für eine solche Separation der Einflussfaktoren ist insbesondere die Aufschlüsselung möglicher Wechselwirkungen der Umwelt-parameter (z.B. Mikroklima an bestrahlten Oberflächen) erforderlich. Einzelne (meist Alterungs-) Empfindlichkeiten können qualitativ nachgewiesen werden oder sogar – als Beanspruchungs-Wirkungs-Funktionen – quantifiziert werden, was einen wesentlichen Schritt in Richtung der Digitalisierung der Material¬prüfung darstellt. Insbesondere ist dann auch eine Lebensdauer-vorhersage für vorgegebene Zeitreihen der Beanspruchungs¬parameter umsetzbar. (iii) Rückwirkungen auf die Umwelt Umweltbeanspruchungen können zur Freisetzung von Schadstoffen in die Umwelt führen. Durch die Nachstellung kritischer, aber realitätsnaher Einsatzszenarien kann die Menge an freigesetzten Substanzen abgeschätzt werden. Egal, welcher Ansatz verfolgt wird – ein Vergleich mit der oder einer Real-beanspruchung ist unerlässlich, ebenso wie die Messdatenaufzeichnung (data logging) aller potenziell relevanten Beanspruchungsparameter während dieser Realbeanspruchung. Obwohl die naturnahe Umwelt – sowohl in der BAM als auch bei der GUS – gegenüber der technischen Umwelt eher untergeordnet auftritt, werden zur Illustration Beispiele aus der naturnahen Umwelt verwendet. T2 - 50. Jahrestagung der GUS CY - Online meeting DA - 23.03.2022 KW - Umweltsimulation PY - 2022 SN - 978-3-9818507-7-2 SP - 79 EP - 89 AN - OPUS4-55015 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - European Food Safety Authority (EFSA), A1 - European Centre for Disease Prevention and Control (ECDC), A1 - European Chemicals Agency (ECHA), A1 - European Environment Agency (EEA), A1 - European Medicines Agency (EMA), A1 - European Commission's Joint Research Centre (JRC), T1 - Annex to: Scientific report 'Impact of the use of azole fungicides, other than as human medicines, on the development of azole-resistant Aspergillus spp.' doi:10.2903/j.efsa.2025.9200 - Annex E - Detailed answer to Term of Reference 5 'Environmental hotspots' and Term of Reference 6 'Prevention and control options' N2 - The widespread use of azole compounds in various sectors has led to the emergence of azole-resistant Aspergillus fumigatus (ARAf), which poses a significant challenge for treating fungal infections, especially in immunocompromised patients. Certain environmental conditions and practices, particularly in agricultural settings and the use of azoles as biocides, have been identified as hotspots for the selection and dispersal of azole-resistant strains of Aspergillus spp. Factors contributing to the selection of resistance include the use of azoles in crop protection, wood preservation and, to a much lesser extent, veterinary medicine. For plant protection products (PPPs), a number of scenarios (green waste of indoor-grown vegetables, uses with the production of wet pomace used as fertiliser, maize or sugar beet silage, and field heaps including flower bulbs) are deemed high risk for hotspot development. Based on EU authorised use patterns, these scenarios are characterised by the hazard characteristics of the azole fungicides in terms of activity against the wild-type Aspergillus spp. compared to resistant strains, substrate characteristics and residue levels, and environmental conditions that promote the growth of the fungus. For biocidal azole applications, products (biocidal product [BP]) for temporary preservation of freshly cut wood have been identified to have the potential for hotspot formation because freshly cut wood allows the growth of Aspergillus spp., and azole concentrations in treated wood are above the predicted no effect concentration (PNEC) for resistance selection (PNECres) and below the minimum inhibitory concentration (MIC) of ARAf for most analysed products on the EU market. Following identification of environmental hotspots, the report recommends measures to prevent the selection of azole-resistant strains in the environment, including controlled storage of organic waste, proper waste management, and responsible use and disposal of azole-treated products. Azole use in veterinary medicinal products (VMPs) represents a very small percentage of total azole use and is unlikely to be a significant source of selection of resistance in the environment. As such, the focus for mitigating resistance should be on other uses of azoles. The report stresses the importance of ongoing surveillance to monitor the presence of ARAf in the environment and to inform risk assessments and management strategies. As industrial chemicals, the azole substances are mostly used as intermediates (precursors) to manufacture yet a different substance, are formulated into a mixture or are reported to be manufactured as active substances in PPP, BP or VMP (therefore already covered above). There are only a few industrial azole substances with widespread use, and as for the moment, there is no evidence from the literature that industrial azoles would be a source of a possible hotspot; thus, the industrial chemicals were not further investigated. There are several areas where further research is needed, including understanding the environmental conditions that support the growth of Aspergillus spp. in different agricultural matrices or on wood, assessing human exposure to resistant strains, regional waste practices and the impact of active substance combinations for azole resistance selection. There is also a need for more comprehensive data on the use and quantities of azole-containing products. Furthermore, industrial substances with widespread use and having antifungal effects, e.g. an antidandruff substance in cosmetics, could be further investigated. Measures were identified that could be implemented with respect to the use of azole fungicides in PPPs as well as in BPs and with respect to the storage, processing and disposal of crop (waste) materials containing azole residues to prevent or minimise the selection of environmental resistance or to minimise the spread of resistant Aspergillus spp. to patients. Any measures that slow down or prevent growth in the presence of azoles, sporulation and dispersal of Aspergillus spp. should be encouraged. A coordinated effort among various stakeholders, including farmers, manufacturers, industrial users, waste managers, regulatory bodies and scientists, is essential to effectively address the challenge of azole resistance in A. fumigatus. KW - Antimicrobial surfaces KW - Biocides KW - Antimicrobial resistance KW - Azoles KW - Fungi KW - Wood preservatives PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-652187 UR - https://doi.org/10.5281/zenodo.14223436 DO - https://doi.org/10.5281/zenodo.14223435 SP - 1 EP - 76 PB - Zenodo CY - Geneva AN - OPUS4-65218 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dinter, Adelina-Elisa ED - Dinter, Adelina-Elisa T1 - Development of a MIC single archaea-ICP-ToF-MS-method for analysis of various elements in solid steel samples N2 - With the latest ICP-MS technology - ICP-ToF (time of flight)-MS - it is possible to analyze the multi-element fingerprint of individual cells. The interface between material and environmental analysis thus receives special attention, e.g. when considering corrosion processes. Microbiologically influenced corrosion (MIC) is highly unpredictable due to the diversity of microbial communities involved. The development of the MIC-specific ICP-ToF-MS analytical method presented here at the single cell level, in combination with the investigation of steel-MIC interactions, contributes significantly to progress in instrumental MIC analysis and will enable clarification of the processes taking place. The supplied document shows the basis of a four minutes lightning talk. T2 - EuroMIC 2021 CY - Online meeting DA - 22.06.2021 KW - SC-ICP-ToF-MS KW - Single cell analysis KW - Microbiologically influenced corrosion KW - Lightning talk PY - 2021 AN - OPUS4-52905 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dinter, Adelina-Elisa T1 - Development of a single cell-ICP-ToF-MS-method for multielement analysis of MIC organisms grown on solid steel samples N2 - The latest ICP-MS technology - ICP-ToF (time of flight)-MS – enables the analysis of the multi-element fingerprint of individual cells. The interface between material and environmental analysis thus receives special attention, e.g., when considering corrosion processes. Microbiologically influenced corrosion (MIC) is a highly unpredictable phenomenon due to the influence of the environment, microbial communities involved and the respective electron source. However, the interaction pathway between cells and the metal surface remains unclear. The development of the MIC-specific ICP-ToF-MS analytical method presented here at the single cell level, in combination with the investigation of steel-MIC interactions, contributes significantly to progress in instrumental MIC analysis and will enable clarification of the processes taking place. For this, a MIC-specific staining procedure was developed. It allows the analysis of archaea at a single cell level and provides information about the interaction of the cells with the staining agent which is extremely scarce compared to other well characterized organisms. Additionally, the single cell ICP-ToF-MS is used for the analysis of archaea involved in MIC of steel. Hence, the possible uptake of individual elements from different steel samples is investigated - the information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts, thus combining modern methods of analytical sciences with materials. T2 - DAAS Doktorandenseminar 2021 CY - Online meeting DA - 20.09.2021 KW - Sc-ICP-ToF-MS KW - Single cell analysis KW - Microbiologically influenced corrosion KW - Archaea PY - 2021 AN - OPUS4-53340 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dinter, Adelina-Elisa T1 - Development of a MIC single archaea-ICP-ToF-MS-method for analysis of various elements in solid steel samples N2 - ICP-ToF (Flugzeitanalysator, engl. time of flight)-MS ermöglicht den Multielement Fingerabdruck einzelner Zellen (single cell) zu analysieren. Die single cell-ICP-ToF-MS kommt bei dem vorgestellten Poster bei der Analyse von Archaeen, die an mikrobiell beeinflusster Korrosion (engl. microbiologically influenced corrosion, MIC) von Stahl eine Rolle spielen, zum Einsatz. Mittels sc-ICP-ToF-MS wird die mögliche Aufnahme von einzelnen Elementen aus dem jeweiligen Stahl untersucht – die erhaltenen Informationen fließen zukünftig in die Aufklärung zugrunde liegender Mechanismen sowie Entwicklung möglicher Materialschutzkonzepte ein. Die Arbeiten Verknüpfen moderne Methoden der Analytical Sciences mit Materialien. T2 - Tag der Chemie 2021 CY - Online meeting DA - 06.07.2021 KW - SC-ICP-ToF-MS KW - Single cell analysis KW - Microbiologically influenced corrosion KW - Archaea PY - 2021 AN - OPUS4-52941 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dinter, Adelina-Elisa T1 - Examination of biological samples by means of single-cell ICP-ToF-MS N2 - Up to now, different analytical methods for single cell analysis exist focusing on key features such as size, shape, morphology and elemental composition. The combination of the latest ICP-MS techniques - ICP-ToF-MS - together with the latest developments in the field of sample delivery - micro droplet generator (MDG) – will allow a Deep insight into the composition and size of cells. Microbiologically influenced corrosion (MIC) is an oxidation of metals affected by the presence or activity (or both) of microorganisms e.g. Shewanella Putrefaciens in biofilms on the surface of the corroding material. As this can happen for example in the soil on iron pipes of water pipes, in oil tanks or on steel sheet piling, there is great interest in MIC research, not only from various industrial sectors, but also from the environmental aspect. T2 - SALSA - Make & Measure CY - Online Meeting DA - 15.10.2020 KW - SC-ICP-ToF-MS KW - Single cell analysis KW - MDG ICP-ToF-MS KW - Microdroplet generator PY - 2020 AN - OPUS4-52441 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dinter, Adelina-Elisa T1 - Deciphering corrosion processes of MIC organisms - single cell-ICP-ToF-MS analysis of archaea on solid steels N2 - ICP-ToF (time of flight) MS enables the analysis of the multi-element fingerprint of single cells. The single cell ICP-ToF-MS is used in the presented poster for the analysis of archaea involved in microbiologically influenced corrosion (MIC) of steel. By means of sc-ICP-ToF-MS, the possible uptake of individual elements from the respective steel is investigated - the information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts. The work combines modern methods of analytical sciences with materials. T2 - SALSA - Make & Measure 2021 CY - Online meeting DA - 16.09.2021 KW - Sc-ICP-ToF-MS KW - Single cell analysis KW - Microbiologically influenced corrosion KW - Archaea KW - Poster presentation PY - 2021 AN - OPUS4-53337 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Deland, Eric A1 - Kleinbub, Sherin T1 - Is Methanogen-induced microbiologically influenced corrosion (Mi-MIC) underestimated? N2 - The role of methanogens in microbiologically influenced corrosion (Mi-MIC) is often neglected, due to 1) low reported corrosion rates and 2) the suspected corrosion product siderite, which is electrically non-conductive. Typically, MIC corrosion studies are carried out using batch cultures, which did not represent the dynamic conditions, i.e. pipeline and provide insufficient information on the overall corrosion potential. We established a unique approach, a multiport flow column (MFC), to simulate pipeline conditions and obtained 10-times higher corrosion rates than previously published. Our result showed that testing procedures have a large impact on the corrosive potential of methanogens. We found strong indications with a combination of ToF-SIMS, SEM-EDS and FIB-SEM analyses that siderite is not the sole corrosion product. The corrosion layers contain phosphorus, oxygen, magnesium, calcium and iron. To verify and deepen our understanding of Mi-MIC, we are currently studying the influence of other environmental parameters (e.g. pH) on Mi-MIC. T2 - VAAM 2021 CY - Online meeting DA - 18.03.2021 KW - Methanogen-induced microbiologically influenced corrosion KW - Carbon steel KW - MIC Island PY - 2021 AN - OPUS4-54042 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Deland, Eric T1 - Environmental conditions affect the corrosion product composition of Methanogen induced microbiologically influenced corrosion (Mi-MIC) N2 - Corrosion is a very expensive and serious problem in the different industry sectors, eg. Petroleum-, On- and off-shore, infrastructure. It is estimated that 20% of all corrosion damage is caused by microorganisms or microbiologically influenced corrosion (MIC). Several microorganisms are known to cause corrosion, including sulfate-reducing bacteria, nitrate-reducing bacteria, methanogens etc. For several years, methanogens were regarded as a mild corroder (~0.065 mm/yr), largely due to a lack of detailed investigation on the corrosion mechanism under real-environment simulated conditions. Resulting in the common belief that siderite, a non-conductive compound, is the sole corrosion product (CP) of methanogen-induced MIC (Mi-MIC). To simulate natural environmental conditions, we developed and introduced the multiport flow column system (MFC), a multi-sectional corrosion flow-cell. Using the MFC, we obtained ten times higher corrosion rates than previously reported. With a combination of several analytical techniques, such as ToF-SIMS, SEM-EDS and FIB-SEM, we found strong indication that siderite is not the sole corrosion product of Mi-MIC. The corrosion layers contained phosphorus, oxygen, magnesium, calcium and iron. The differences in the CP between static and dynamic environments demonstrated the impact of testing procedures on the corrosive potential of methanogens. To further verify and deepen our understanding of Mi-MIC, we are currently studying the influence of additional environmental parameters (e.g. pH, salinity, flow rate) on Mi-MIC. Overall, results of this study will expand the current understanding of MIC from both analytical and mechanistic points of view, thus aiding the development of different mitigation strategies for various industry sectors. T2 - ISMOS-8 CY - Online meeting DA - 08.06.2021 KW - Methanogen-induced microbiologically influenced corrosion KW - Carbon steel KW - ToF-SIMS KW - Modelling studies PY - 2021 AN - OPUS4-54041 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Deland, Eric T1 - Environmental conditions impact the corrosion layer composition of Methanogen induced microbiologically influenced corrosion (Mi-MIC) N2 - Microbiologically influenced corrosion (MIC) is a now well-known challenge and affects industry, society and infrastructure. For a long time, the impact of methanogen-induced MIC (Mi-MIC) was underestimated. This was mainly due to the rather low published corrosion rates and the presumed corrosion product siderite, which is not electrically conductive. In our laboratory, we were able to show that this trivialization or underestimation of Mi-MIC was due to the nature of the testing. The static systems used so far do not provide environmentally relevant information about the corrosion rate and the corrosion product, especially for methanogens To further illustrate the importance of the environment on the MIC process, we established a multiport flow column (MFC), to simulate the natural environment. With this method, and in contrast to published results using the static conditions, we obtained ten times higher corrosion rates. We analyzed the corrosion products with a combination of techniques, like ToF-SIMS, SEM-EDS and FIB-SEM, and found strong evidence that siderite is not the sole corrosion product of Mi-MIC. The corrosion layers contain phosphorus, oxygen, magnesium, calcium and iron and lacked on carbon-related species. It is hypothesized that methanogens may have influenced the nucleation process of siderite, converting bicarbonate into carbon dioxide for methanogenesis. This results in increased localized corrosion and reduced siderite formation. To verify and deepen our understanding of Mi-MIC, we are currently studying the influence of additional environmental parameters (e.g. pH, salinity, flow rate etc.) on Mi-MIC and the subsequent impacts on corrosion rates and the corrosion products. Overall, results of this study will expand the current understanding of MIC from both analytical and mechanistic points of view, thus aiding the development of different mitigation strategies for various industry sectors. T2 - Eurocorr 2021 CY - Online meeting DA - 20.09.2021 KW - Methanogen-induced microbiologically influenced corrosion KW - Carbon steel KW - SEM-EDS KW - Modelling studies PY - 2021 AN - OPUS4-54043 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - de Araujo Abilio, André A1 - Wolodko, John D. A1 - Eckert, Richard B. A1 - Skovhus, Torben L. T1 - Development of an expert system for assessing failures in oil and gas pipelines due to microbiologically influenced corrosion (MIC) N2 - This study highlights the modeling of an expert system for the classification of internal microbiologically influenced corrosion (MIC) failures related to pipelines in the upstream oil and gas industry. The model is based on artificial neural networks (ANNs) and involves the participation of 15 MIC experts. Each expert evaluated a number of model case studies ranging from MIC- to non-MIC-driven upstream pipeline failures. The model accounts for variations in microbiological testing methods, microbiological sample types, and degradation morphology, among other variables. It also accounts for missing datasets, which is commonly the case in actual failure assessments. The outcome is an expert system model whose outputs are classes (classification ANN) which comprises MIC potential and data confidence. The performances of two approaches are contrasted in this study. One classifies the output in 5 classes, a 5-output classification (5OC) model; the other in 3 classes, a 3-output classification (3OC) model. The 5OC model had an accuracy of 62.0% while the simpler 3OC model had a better accuracy of 74.8%. This modelling exercise has demonstrated that knowledge from experts can be captured in a reasonably effective model to screen for possible MIC failures. It is hoped that this study not only may contribute to a better understanding of the prevalence of MIC in the oil and gas sector, but also may highlight the key areas necessary to improve the diagnosis of MIC failures in the future. KW - Corrosion engineering KW - Oil and gas pipelines KW - Carbon steel KW - Artificial neural networks (ANNs) KW - Failure analysis KW - Microbiologically influenced corrosion (MIC) KW - Expert systems PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-632084 DO - https://doi.org/10.1016/j.engfailanal.2024.108426 SN - 1350-6307 VL - 163 SP - 1 EP - 16 PB - Elsevier Ltd. AN - OPUS4-63208 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Braymer, Joseph T1 - Methanobacterium IM1 as a model organism to study MIC N2 - Microbiologically influenced corrosion (MIC), also known as biocorrosion, is a process where microbes corrode metal surfaces causing detrimental damage to manmade infrastructure. Several studies, mainly under anaerobic conditions, have identified sulfate-reducing bacteria and methanogens as key players in biocorrosion. However, an important issue is that most MIC-related microbes at the moment are non-culturable. In addition, improved technologies (i.e., omics, biochemical, and microscopy methods) are needed to study, detect, simulate, and modulate MIC-related cases. Therefore, the selection of strains that can cause MIC, are culturable, and are genetically accessible, are of high importance for establishing new methods to study MIC on the molecular level. For example, the archaeal strain Methanobacterium IM1 has caught great attention due to its involvement in biocorrosion processes. Genes encoding for a novel enzyme (MIC NiFe-hydrogenase) have been proposed in this methanogen to be directly related to the e T2 - International Biodeterioration and Biodegradation Symposium 19 CY - Berlin, Germany DA - 11.09.2024 KW - Biocorrosion KW - Methanogen KW - Iron KW - Hydrogenase KW - Mechanism PY - 2024 AN - OPUS4-61508 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn A1 - Schwibbert, Karin T1 - Laserstrukturierte Oberflächen: Ein Weg zur Kontrolle der Biofilmbildung? N2 - Bakterielle Biofilme stellen in medizinischen und industriellen Bereichen ein ernsthaftes Problem dar. Eine der größten gesellschaftlichen Herausforderungen liegt in der zunehmenden Resistenz von Bakterien gegen Biozide, die bei antimikrobiellen Behandlungen eingesetzt werden, z.B. durch übermäßigen Einsatz in Medizin, Industrie und Landwirtschaft oder durch Reinigung und Desinfektion in Privathaushalten. Daher sind neue effiziente bakterienabweisende Strategien, die den Einsatz von Bioziden vermeiden, dringend erforderlich. Ein vielversprechender Weg zur Erzielung bakterienabweisender Oberflächen liegt in der berührungslosen und aseptischen großflächigen Laserbearbeitung von technischen Oberflächen. Maßgeschneiderte Oberflächentexturen, ermöglicht durch verschiedene Laserbearbeitungsstrategien, die zu topographischen Skalen im Bereich von Nanometern bis Mikrometern führen, können eine Lösung für diese Herausforderung darstellen. In dem Vortrag wird ein Überblick über den aktuellen Stand der Technik bei der subtraktiven Texturierung von Laseroberflächen zur Kontrolle der Biofilmbildung bei verschiedenen Bakterienstämmen und in unterschiedlichen Umgebungen gegeben. Auf der Grundlage spezifischer Eigenschaften von Bakterien und laserbearbeiteten Oberflächen werden die Herausforderungen antimikrobieller Oberflächendesigns erörtert und zukünftige Richtungen aufgezeigt. T2 - GRAVOSeminar, GRAVOmer Kompetenznetzwerk CY - Online meeting DA - 28.02.2024 KW - Laser-Materialbearbeitung KW - Ultrakurzpuls-Laser KW - Biofilme KW - Antibakterielle Oberflächen KW - Oberflächenfunktionalisierung PY - 2024 UR - https://gravomer.de/veranstaltungen-anzeigen/laserstrukturierte-oberflaechen-ein-weg-zur-kontrolle-der-biofilmbildung AN - OPUS4-59594 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn T1 - A brief survey on open questions about laser-induced periodic surface structures N2 - The processing of laser-induced periodic surface structures (LIPSS) represents a simple and robust way for the nanostructuring of solids that allows creating a wide range of surface functionalities featuring applications in optics, tribology, medicine, energy technologies, etc. While the currently available laser and scanner technology already allows surface processing rates at the m2/min level, industrial applications of LIPSS are sometimes hampered by the complex interplay between the nanoscale surface topography and the specific surface chemistry. This typically manifests in difficulties to control the processing of LIPSS and in limitations to ensure the long-term stability of the created surface functions. This presentation aims to identify some unsolved scientific problems related to LIPSS, discusses the pending technological limitations, and sketches the current state of theoretical modelling. Hereby, it is intended to stimulate further research and developments in the field of LIPSS for overcoming these limitations and for supporting the transfer of the LIPSS technology into industry. T2 - E-MRS Spring Meeting 2022 CY - Online meeting DA - 30.05.2022 KW - Laser-induced periodic surface structures (LIPSS) KW - Surface functionalization KW - Industrial applications KW - Biofilm growth PY - 2022 AN - OPUS4-54929 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn T1 - Impact of laser-induced periodic surface structures on the bactericidal properties of copper and brass N2 - Surfaces of metallic copper and copper alloys effectively inactivate microorganisms and viruses. However, the exact inactivation mode is still under debate. Main factors are assumed to include direct contact with the metallic surface, influx of Cu(I)/Cu(II) ions and the generation of reactive oxygen species (ROS). Laser-induced periodic surface structures (LIPSS) are frequently reported to act antibacterial, mainly by prevention of bacterial adhesion due to a limited number of possible adhesion points or by increasing the overall surface of intrinsically antibacterial materials. In time-kill experiments with E. coli and S. aureus we analyzed the impact of LIPSS on the toxicity of metallic copper and brass. We also conducted ROS accumulation assays and conclude that the application of LIPSS is not generally straight forward to obtain or improve antibacterial surfaces. Thus, the antibacterial effects of LIPPS. T2 - 2023 Spring Meeting CY - Strasbourg, France DA - 29.05.2023 KW - LIPSS KW - Antimicrobial KW - Reactive oxygen species PY - 2023 AN - OPUS4-58465 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn T1 - Bacterial adhesion on ultrashort pulse laser processed surfaces ― more than size matters! N2 - Bacterial biofilms are aggregates of bacterial cells, often attached to a surface and enclosed by a self-produced extracellular matrix which confers increased stress tolerance and resistance to cleaning. Biofilm formation leads to biofouling which gives rise to high costs in numerous technical settings due to biocorrosion and biodegradation. However, biofilms can also be attractive for industrial settings such as wastewater treatment systems or for soil bioremediation processes. Hence, the control of bacterial adhesion to a surface is of major concern. Surface topography strongly influences bacterial adhesion. Therefore, one promising way to achieve bacteria-guiding surfaces lies in the contactless and aseptic large-area laser processing of technical surfaces. We used short and ultrashort pulsed laser systems to generate different surface textures, mainly high-spatial-frequency and low-spatial-frequency laser-induced periodic surface structures, LIPSS (HFSL and LFSL), on Ti, Ti-alloy, steel, and polymers (PET and PE). Pristine (polished) and laser processed samples were subjected to bacterial adhesion experiments with two different Escherichia coli strains and Staphylococcus aureus as test organisms. The bacterial strains differed in their cell wall structure (grampositive vs. gramnegative strains), in size, shape, the occurrence of cell appendages, and in their biofilm forming capabilities. Adhesion patterns were analyzed microscopically and compared regarding the respective test strain and surface topography. Our results revealed that adhesion behavior strongly depends not only on the material’s topography and chemistry, but also on the specific bacterial strain, the presence of cell appendages, and ambient growth conditions. T2 - 13th International Conference on Photoexcited Processes and Applications, ICPEPA-13 CY - Lecce, Italy DA - 14.09.2025 KW - Biofilm KW - Bacterial adhesion KW - Laser-induced periodic surface structures (LIPSS) KW - Ultrashort laser pulses PY - 2025 AN - OPUS4-64166 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn T1 - Bacterial adhesion on ultrashort pulse laser processed surfaces ― more than size matters! N2 - Bacterial biofilms are aggregates of bacterial cells, often attached to a surface, and enclosed by a self-produced extracellular matrix which confers increased stress tolerance and resistance to cleaning. Biofilm formation leads to biofouling which gives rise to high costs in numerous technical settings due to biocorrosion and biodegradation. However, biofilms can also be attractive for industrial settings such as wastewater treatment systems or for soil bioremediation processes. Hence, the control of bacterial adhesion to a surface is of major concern. Surface topography strongly influences bacterial adhesion. Therefore, one promising way to achieve bacteria-guiding surfaces lies in the contactless and aseptic large-area laser processing of technical surfaces. We used short and ultrashort pulsed laser systems to generate different surface textures, mainly high-spatial-frequency and low-spatial-frequency laser-induced periodic surface structures, LIPSS (HFSL and LFSL), on Ti, Ti-alloy, steel, and polymers (PET and PE). Pristine (polished) and laser processed samples were subjected to bacterial adhesion experiments with two different Escherichia coli strains and Staphylococcus aureus as test organisms. The bacterial strains differed in their cell wall structure (grampositive vs. gramnegative strains), in size, shape, the occurrence of cell appendages, and in their biofilm forming capabilities. Adhesion patterns were analyzed microscopically and compared regarding the respective test strain and surface topography. Our results revealed that adhesion behavior strongly depends not only on the material’s topography and chemistry, but also on the specific bacterial strain, the presence of cell appendages, and ambient growth conditions. T2 - 13th International LIPSS Workshop CY - Enschede, Netherlands DA - 29.10.2025 KW - Bacterial adhesion KW - Biofilm KW - Laser-induced periodic surface structures (LIPSS) KW - Ultrashort laser pulses PY - 2025 AN - OPUS4-64632 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Barkeshli, M. A1 - Stephan, Ina ED - Barkeshli, M. ED - Arya, A. ED - Nath, V. ED - Abduraheem, K. T1 - Sizing materials used in Persian manuscripts and their behaviour against the mould fungus Aspergillus flavus N2 - Our earlier study based on historical and scientific analysis showed that Iranians used a considerable range of materials in the sizing process. Unlike many nations who used limited sizing materials to improve the mechanical strength and to smoothen the paper surface, Iranians have used various materials for sizing process from Taimurid (15th century) to Safawid (16th century) and Qajar (19th century) periods. Further to our earlier study on the Sizing Materials used in Persian manuscripts and miniature paintings, scientific analysis was carried out to investigate their behaviour against the mould fungus Aspergillus flavus. In the first stage fourteen different sizing materials that was identified from Persian historical recipes were reconstructed. In the second stage fungicidal property of each sizing samples against Aspergillus flavus fungus were identified that will be presented in this paper. KW - Sizing KW - Materials KW - Persian manuscripts KW - Deterioration KW - Fungus KW - Aspergillus flavus PY - 2023 SN - 978-93-5461-714-0 SP - 3 EP - 20 PB - Daya Publishing House CY - New Delhi AN - OPUS4-58102 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - An, Biwen Annie A1 - Kunte, Hans-Jörg A1 - Koerdt, Andrea ED - Eibergen, N. ED - Poulassichidis, T. T1 - Microbiologically Influenced Corrosion (MIC) by Halophilic (Salt-Loving) Nitrate and Sulfate-Reducing Microorganisms N2 - The survey of Canadian shale sites showed a dominance of halophilic microorganisms, including Halomonas (HA). Nitrate-amended incubations of the field samples under high salinity (14.6% NaCl), revealed a dominance of HA (>72%) and an accumulation of nitrite. Nitrite accumulation directly inhibited the growth of SRB, thereby decreasing their souring and corrosion risks. However, accumulated nitrite may also contribute to iron corrosion, which will be tested by using different concentrations of nitrate as an electron acceptor to HA. Different salinities are further tested on HA strains supplemented with iron coupons to determine their effects on iron corrosion rates. HA incubated with separate cultures of corrosive methanogen and SRB were tested to determine whether a positive or adverse effect will occur between them. Lastly, analyses of iron coupons will be conducted using TOF-SIMS, FIB-SEM and EDS for corrosion product characterization T2 - Corrosion 2021 CY - Online Meeting DA - 19.04.2021 KW - MIC KW - Bacteria KW - Halophile KW - Corrosion KW - Environmental condition KW - Korrosion KW - High salinity PY - 2021 UR - https://my.nace.org/PaperTrail/Authors/Submission.aspx?id=2914f145-7f8f-ea11-813a-005056a95a7c SP - Paper C2021-16284, 1 AN - OPUS4-52479 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -