TY - JOUR
A1 - Hobmeier, K.
A1 - Oppermann, M.
A1 - Stasinski, N.
A1 - Kremling, A.
A1 - Pflüger-Grau, K.
A1 - Kunte, Hans-Jörg
A1 - Marin Sanguino, A.
T1 - Metabolic engineering of Halomonas elongata: Ectoine secretion is increased by demand and supply driven approaches
JF - Frontiers in Microbiology
N2 - The application of naturally-derived biomolecules in everyday products, replacing conventional synthetic manufacturing, is an ever-increasing market. An example of this is the compatible solute ectoine, which is contained in a plethora of treatment formulations for medicinal products and cosmetics. As of today, ectoine is produced in a scale of tons each year by the natural producer Halomonas elongata. In this work, we explore two complementary approaches to obtain genetically improved producer strains for ectoine production. We explore the effect of increased precursor supply (oxaloacetate) on ectoine production, as well as an implementation of increased ectoine demand through the overexpression of a transporter. Both approaches were implemented on an already genetically modified ectoine-excreting strain H. elongata KB2.13 (ΔteaABC ΔdoeA) and both led to new strains with higher ectoine excretion. The supply driven approach led to a 45% increase in ectoine titers in two different strains. This increase was attributed to the removal of phosphoenolpyruvate carboxykinase (PEPCK), which allowed the conversion of 17.9% of the glucose substrate to ectoine. For the demand driven approach, we investigated the potential of the TeaBC transmembrane proteins from the ectoine-specific Tripartite ATP-Independent Periplasmic (TRAP) transporter as export channels to improve ectoine excretion. In the absence of the substrate-binding protein TeaA, an overexpression of both subunits TeaBC facilitated a three-fold increased excretion rate of ectoine. Individually, the large subunit TeaC showed an approximately five times higher extracellular ectoine concentration per dry weight compared to TeaBC shortly after its expression was induced. However, the detrimental effect on growth and ectoine titer at the end of the process hints toward a negative impact of TeaC overexpression on membrane integrity and possibly leads to cell lysis. By using either strategy, the ectoine synthesis and excretion in H. elongata could be boosted drastically. The inherent complementary nature of these approaches point at a coordinated implementation of both as a promising strategy for future projects in Metabolic Engineering. Moreover, a wide variation of intracelllular ectoine levels was observed between the strains, which points at a major disruption of mechanisms responsible for ectoine regulation in strain KB2.13.
KW - Osmoadaptation
KW - Metabolic engineering
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-555644
DO - https://doi.org/10.3389/fmicb.2022.968983
SN - 1664-302X
VL - 13
SP - 1
EP - 13
PB - Frontiers Media
CY - Lausanne
AN - OPUS4-55564
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Hobmeier, K.
A1 - Cantone, M.
A1 - Nguyen, Q. A.
A1 - Pflüger-Grau, K.
A1 - Kremling, A.
A1 - Kunte, Hans-Jörg
A1 - Pfeiffer, F.
A1 - Marin-Sanguino, A.
T1 - Adaptation to varying salinity in Halomonas elongata: Much more than ectoine accumulation
JF - Frontiers in microbiology
N2 - The halophilic γ-proteobacterium Halomonas elongata DSM 2581T thrives at salt concentrations well above 10 % NaCl (1.7 M NaCl). A well-known osmoregulatory mechanism is the accumulation of the compatible solute ectoine within the cell in response to osmotic stress. While ectoine accumulation is central to osmoregulation and promotes resistance to high salinity in halophilic bacteria, ectoine has this effect only to a much lesser extent in non-halophiles. We carried out transcriptome analysis of H. elongata grown on two different carbon sources (acetate or glucose), and low (0.17 M NaCl), medium (1 M), and high salinity (2 M) to identify additional mechanisms for adaptation to high saline environments. To avoid a methodological bias, the transcripts were evaluated by applying two methods, DESeq2 and Transcripts Per Million (TPM). The differentially transcribed genes in response to the available carbon sources and salt stress were then compared to the transcriptome profile of Chromohalobacter salexigens, a closely related moderate halophilic bacterium. Transcriptome profiling supports the notion that glucose is degraded via the cytoplasmic Entner-Doudoroff pathway, whereas the Embden-Meyerhoff-Parnas pathway is employed for gluconeogenesis. The machinery of oxidative phosphorylation in H. elongata and C. salexigens differs greatly from that of non-halophilic organisms, and electron flow can occur from quinone to oxygen along four alternative routes. Two of these pathways via cytochrome bo' and cytochrome bd quinol oxidases seem to be upregulated in salt stressed cells. Among the most highly regulated genes in H. elongata and C. salexigens are those encoding chemotaxis and motility proteins, with genes for chemotaxis and flagellar assembly severely downregulated at low salt concentrations. We also compared transcripts at low and high-salt stress (low growth rate) with transcripts at optimal salt concentration and found that the majority of regulated genes were down-regulated in stressed cells, including many genes involved in carbohydrate metabolism, while ribosome synthesis was up-regulated, which is in contrast to what is known from non-halophiles at slow growth. Finally, comparing the acidity of the cytoplasmic proteomes of non-halophiles, extreme halophiles and moderate halophiles suggests adaptation to an increased cytoplasmic ion concentration of H. elongata. Taken together, these results lead us to propose a model for salt tolerance in H. elongata where ion accumulation plays a greater role in salt tolerance than previously assumed.
KW - Ectoine
KW - Osmoadaptation
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-545608
DO - https://doi.org/10.3389/fmicb.2022.846677
SN - 1664-302X
VL - 13
SP - 1
EP - 19
PB - Frontiers Media
CY - Lausanne
AN - OPUS4-54560
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Gugin, Nikita
A1 - Villajos Collado, José Antonio
A1 - Feldmann, Ines
A1 - Emmerling, Franziska
T1 - Mix and wait – a relaxed way for synthesizing ZIF-8
JF - RSC Advances
N2 - Herein we report the synthesis of a zeolitic imidazolate framework (ZIF-8) by an easy “mix and wait” procedure. In a closed vial, without any interference, the mixture of 2-methylimidazole and basic zinc carbonate assembles into the crystalline product with approx. 90% conversion after 70 h. The reaction exhibits sigmoidal kinetics due to the self-generated water which accelerates the reaction.
KW - In-situ analysis
KW - Mechanochemistry
KW - MOF
KW - Synthesis
KW - ZIF-8
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-546841
DO - https://doi.org/10.1039/D2RA00740A
VL - 12
SP - 8940
EP - 8944
PB - Royal Society of Chemistry
AN - OPUS4-54684
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Golian, M.
A1 - Bien, T.
A1 - Schmelzle, S.
A1 - Esparza- Mora, M. A.
A1 - McMahon, Dino Peter
A1 - Dreisewerd, K.
A1 - Buellesbach, J.
ED - Appel, Arthur G.
T1 - Neglected Very Long-Chain Hydrocarbons and the Incorporation of Body Surface Area Metrics Reveal Novel Perspectives for Cuticular Profile Analysis in Insects
JF - Insects
N2 - Most of our knowledge on insect cuticular hydrocarbons (CHCs) stems from analytical techniques based on gas-chromatography coupled with mass spectrometry (GC-MS). However, this method has its limits under standard conditions, particularly in detecting compounds beyond a chain length of around C40. Here, we compare the CHC chain length range detectable by GC-MS with the range assessed by silver-assisted laser desorption/ionization mass spectrometry (Ag-LDI-MS), a novel and rarely applied technique on insect CHCs, in seven species of the order Blattodea. For all tested species, we unveiled a considerable range of very long-chain CHCs up to C58, which are not detectable by standard GC-MS technology. This indicates that general studies on insect CHCs May frequently miss compounds in this range, and we encourage future studies to implement analytical techniques extending the conventionally accessed chain length range. Furthermore, we incorporate 3D scanned insect body surface areas as an additional factor for the comparative quantification of extracted CHC amounts between our study species. CHC quantity distributions differed considerably when adjusted for body surface areas as opposed to directly assessing extracted CHC amounts, suggesting that a more accurate evaluation of relative CHC quantities can be achieved by taking body surface areas into account.
KW - Cuticular hydrocarbons
KW - Blattodea
KW - GC-MS
KW - Ag-LDI-MS
KW - Chemical ecology
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-543530
DO - https://doi.org/10.3390/insects13010083
VL - 13
IS - 1
SP - 2
EP - 10
PB - MDPI
CY - Basel, Schweiz
AN - OPUS4-54353
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Gentzmann, Marie
A1 - Paul, Andrea
A1 - Serrano, Juan
A1 - Adam, Christian
T1 - Understanding scandium leaching from bauxite residues of different geological backgrounds using statistical design of experiments
JF - Journal of Geochemical Exploration
N2 - The leaching behavior of scandium (Sc) from bauxite residues can differ significantly when residues of different geological backgrounds are compared. The mineralogy of the source rock and the physicochemical environment during bauxitization affect the association of Sc in the bauxite i.e., how Sc is distributed amongst different mineral phases and whether it is incorporated in and/or adsorbed onto those phases. The Sc association in the bauxite is in turn crucial for the resulting Sc association in the bauxite residue. In this study systematic leaching experiments were performed on three different bauxite residues using a statistical design of experiments approach. The three bauxite residues compared originated from processing of lateritic and karstic bauxites from
Germany, Hungary, and Russia. The recovery of Sc and Fe was determined by ICP-OES measurements. Mineralogical changes were analyzed by X-ray-diffraction and subsequent Rietveld refinement. The effects of various parameters including temperature, acid type, acid concentration, liquid-to-solid ratio and residence time were studied. A response surface model was calculated for the selected case of citric acid leaching of Hungarian bauxite residue. The investigations showed that the type of bauxite residue has a strong influence. The easily leachable fraction of Sc can vary considerably between the types, reaching ~20–25% in German Bauxite residue and ~50% in Russian bauxite residue. Mineralogical investigations revealed that a major part of this fraction was released from secondary phases such as cancrinite and katoite formed during Bayer processing of the bauxite.
The effect of temperature on Sc and Fe recovery is strong especially when citric acid is used. Based on the exponential relationship between temperature and Fe-recovery it was found to be particularly important for the selectivity of Sc over Fe. Optimization of the model for a maximum Sc recovery combined with a minimum Fe
recovery yielded results of ~28% Sc recovery at <2% Fe recovery at a temperature of 60 ◦C, a citric acid normality of 1.8, and a liquid-to-solid ratio of 16 ml/g. Our study has shown that detailed knowledge about the Sc association and distribution in bauxite and bauxite residue is key to an efficient and selective leaching of Sc
from bauxite residues.
KW - Bauxite residue
KW - Scandium
KW - Leaching
KW - Design of experiments
KW - Red mud
PY - 2022
DO - https://doi.org/10.1016/j.gexplo.2022.107041
SN - 0375-6742
VL - 2022
IS - 240
SP - 1
EP - 13
PB - Elsevier Science
CY - Amsterdam
AN - OPUS4-55531
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Erdmann, Eileen
A1 - Nitsche, Sarah
A1 - Gorbushina, Anna
A1 - Schumacher, Julia
T1 - Genetic Engineering of the Rock Inhabitant Knufia petricola Provides Insight Into the Biology of Extremotolerant Black Fungi
JF - Frontiers in Fungal Biology
N2 - Black microcolonial fungi (Ascomycetes from Arthonio-, Dothideo-, and Eurotiomycetes) are stress-tolerant and persistent dwellers of natural and anthropogenic extreme habitats. They exhibit slow yeast-like or meristematic growth, do not form specialized reproduction structures and accumulate the black pigment 1,8-dihydroxynaphthalene (DHN) melanin in the multilayered cell walls. To understand how black fungi live, survive, colonize mineral substrates, and interact with phototrophs genetic methods are needed to test these functions and interactions. We chose the rock inhabitant Knufia petricola of the Chaetothyriales as a model for developing methods for genetic manipulation. Here, we report on the expansion of the genetic toolkit by more efficient multiplex CRISPR/Cas9 using a plasmid-based system for expression of Cas9 and multiple sgRNAs and the implementation of the three resistance selection markers genR (geneticin/nptII), baR (glufosinate/bar), and suR (chlorimuron ethyl/sur). The targeted integration of expression constructs by replacement of essential genes for pigment synthesis allows for an additional color screening of the transformants. The black-pink screening due to the elimination of pks1 (melanin) was applied for promoter studies using GFP fluorescence as reporter. The black-white screening due to the concurrent elimination of pks1 and phs1 (carotenoids) allows to identify transformants that contain the two expression constructs for co-localization or bimolecular fluorescence complementation (BiFC) studies. The co-localization and interaction of the two K. petricola White Collar orthologs were demonstrated. Two intergenic regions (igr1, igr2) were identified in which expression constructs can be inserted without causing obvious phenotypes. Plasmids of the pNXR-XXX series and new compatible entry plasmids were used for fast and easy generation of expression constructs and are suitable for a broad implementation in other fungi. This variety of genetic tools is opening a completely new perspective for mechanistic and very detailed study of expression, functioning and regulation of the genes/proteins encoded by the genomes of black fungi.
KW - Microcolonial fungi
KW - DHN melanin
KW - Cloning vectors
KW - Genetics
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-546192
DO - https://doi.org/10.3389/ffunb.2022.862429
SN - 2673-6128
VL - 3
PB - Frontiers Media
CY - Lausanne
AN - OPUS4-54619
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Dombinov, V.
A1 - Herzel, Hannes
A1 - Meiller, M.
A1 - Müller, F.
A1 - Willbold, S.
A1 - Zang, J. W.
A1 - da Fonseca-Zang, W. A.
A1 - Adam, Christian
A1 - Klose, H.
A1 - Poorter, H.
A1 - Jablonowski, N. D.
A1 - Schrey, S. D.
T1 - Sugarcane bagasse ash as fertilizer for soybeans: Effects of added residues on ash composition, mineralogy, phosphorus extractability and plant availability
JF - Frontiers in Plant Science
N2 - Sugarcane bagasse is commonly combusted to generate energy. Unfortunately, recycling strategies rarely consider the resulting ash as a potential fertilizer. To evaluate this recycling strategy for a sustainable circular economy, we characterized bagasse ash as a fertilizer and measured the effects of co-gasification and co-combustion of bagasse with either chicken manure or sewage sludge: on the phosphorus (P) mass fraction, P-extractability, and mineral P phases. Furthermore, we investigated the ashes as fertilizer for soybeans under greenhouse conditions. All methods in combination are reliable indicators helping to assess and predict P availability from ashes to soybeans. The fertilizer efficiency of pure bagasse ash increased with the ash amount supplied to the substrate. Nevertheless, it was not as effective as fertilization with triple-superphosphate and K2SO4, which we attributed to lower P availability. Co-gasification and co-combustion increased the P mass fraction in all bagasse-based ashes, but its extractability and availability to soybeans increased only when co-processed with chicken manure, because it enabled the formation of readily available Ca-alkali phosphates. Therefore, we recommend co-combusting biomass with alkali-rich residues to increase the availability of P from the ash to plants.
KW - Combustion and gasification
KW - Phosphate extractability and availability
KW - X-ray diffraction (XRD)
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-567632
DO - https://doi.org/10.3389/fpls.2022.1041924
SN - 1664-462X
VL - 13
SP - 1
EP - 13
PB - Frontiers Media
CY - Lausanne
AN - OPUS4-56763
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Cros, A.
A1 - Alfaro Espinoza, Gabriela
A1 - De Maria, A.
A1 - Wirth, N. T.
A1 - Nikel, P. I.
T1 - Synthetic metabolism for biohalogenation
JF - Current opinion in biotechnology
N2 - The pressing need for novel bioproduction approaches faces a limitation in the number and type of molecules accessed through synthetic biology. Halogenation is widely used for tuning physicochemical properties of molecules and polymers, but traditional halogenation chemistry often lacks specificity and generates harmful by-products. Here, we pose that deploying synthetic metabolism tailored for biohalogenation represents an unique opportunity towards economically attractive and environmentally friendly organohalide production. On this background, we discuss growth-coupled selection of functional metabolic modules that harness the rich repertoire of biosynthetic and biodegradation capabilities of environmental bacteria for in vivo biohalogenation. By rationally combining these approaches, the chemical landscape of Living cells can accommodate bioproduction of added-value organohalides which, as of today, are obtained by traditional chemistry.
KW - Halogenation
KW - Synthetic metabolism
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-542013
DO - https://doi.org/10.1016/j.copbio.2021.11.009
SN - 0958-1669
SN - 1879-0429
VL - 74
SP - 180
EP - 193
PB - Elsevier
CY - Amsterdam
AN - OPUS4-54201
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Chubarenko, B.
A1 - Kileso, A.
A1 - Esiukova, E.
A1 - Pinchuk, V.
A1 - Simon, Franz-Georg
T1 - Dataset on geosynthetic material debris contamination of the South-East Baltic shore
JF - Data in Brief
N2 - The database gives information on the contamination of the shore of the South-Eastern Baltic with the debris of geosynthetic materials for the period 2018–2020. This new type of coastal pollution enters the natural environment due to the destruction of coastal protection structures and construction activities. The database contains sections:
(1) a list of types of geosynthetic material residues, their photographic images and photographs illustrating examples of finds in natural conditions
[1 List_geosynthetic_debris_SEB],
(2) monitoring data on the contamination of the beach strip with the debris of geotextiles, braids from gabions, geocontainers (big bags), geocells and geogrids for the beaches of the South-Eastern Baltic for the period 2018–2020
[2 Monitoring_geosynthetic_debris_SEB];
(3) statistical distributions of the found geosynthetic debris by size
[3 Scales_geosynthetic_debris_SEB] and (4) results of test surveys on the shores of Lithuania and Poland adjacent to Kaliningrad Oblast.
All data refer to the beaches of the Kaliningrad Oblast (Russia), including the Russian parts of the Vistula and Curonian Spits, but also contains information on a one-time assessment of the pollution of the beaches of the adjacent territories: the Polish shore from the Poland-Russia border on the Vistula Spit to the mouth of the Vistula River, the Lithuanian shore from the border Lithuania-Russia on the Curonian Spit to the border of Latvia-Lithuania. Materials were collected during field surveys within the ERANET-RUS_Plus joint project EI-GEO, ID 212 (RFBR 18-55-76002 ERA_a, BMBF 01DJ18005).
KW - Geosynthetics
KW - Geotextiles
KW - Contamination
KW - Marine littering
PY - 2022
UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-541947
DO - https://doi.org/10.1016/j.dib.2021.107778
SN - 2352-3409
VL - 40
SP - 1
EP - 7
PB - Elsevier Inc.
CY - Amsterdam
AN - OPUS4-54194
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -
TY - JOUR
A1 - Brischke, C.
A1 - Bollmus, S.
A1 - Melcher, E.
A1 - Stephan, Ina
T1 - Biological durability and moisture ynamics of Dawn redwood (Metasequoia glyptostroboides) and Port Orford cedar (Chamaecyparis lawsoniana)
JF - Wood Material Science and Engineering
N2 - Numerous non-native tree species are given attention with respect to the reforestation of calamity areas in Europe. Among them, several species may form durable wood which can be used for outdoor applications, but differences in wood durability are expected between original and European growth sites. This study aimed at examining the biological durability against wooddestroying fungi and water permeability of German-grown Dawn redwood (Metasequoia glyptostroboides) and Port Orford cedar (Chamaecyparis lawsoniana). The heartwood of both wood species was assigned to durability class 4 (DC 4, less durable) in soil contact and DC 1–4 (very to less durable) against wood-destroying basidiomycetes. However, according to the Meyer-Veltrup model, their material resistance dose was notably higher compared to the reference species Norway spruce (Picea abies), and the resulting service life of above ground structures should be a multiple of the reference.
KW - Natural durability
KW - Fungal decay
KW - Moisture performance
KW - Permeability
KW - Resistance model
KW - Water uptake
PY - 2022
DO - https://doi.org/10.1080/17480272.2022.2101941
SN - 1748-0272
SP - 1
EP - 11
PB - Taylor & Francis
CY - London
AN - OPUS4-55490
LA - eng
AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER -