TY - CONF A1 - Schmidt, Selina T1 - Biocides Used as Material Preservatives Modify Rates of De Novo Mutation And Horizontal Gene Transfer in Bacteria N2 - Antimicrobial resistance (AMR) is an important global health problem. The environment has been regocnized as an improtant compartment for the occurance, evolution and transmission of AMR. Biocides used as material preservatives are in contact with the environment and natural microbial communities through direct application and passive leaching from protected materials. It has been shown that environmental contaminants, such as antibiotics, metals and pesticides, can affect resistance evolution and spread by modifying the underlying processes of de novo mutagenesis, horizontal gene transfer and selection. However, it is unknown if material preservatives are involved in these processes and thereby can also drive AMR in the environment. Here, we investigate the effect of material preservatives on rates of de novo mutation and horizontal gene transfer (HGT) in Escherichia coli and the model soil microorganisms Acinetobacter baylyi and Bacillus subtilis. To this end, we determined the effects of material preservatives on the mutation rates and HGT with the Luria-Delbrück fluctuation assay and a conjugation assay with the broad host-range plasmid pKJK5 and a transformation assay. Our data shows that the quaternary ammonium compound DDAC, copper, the pyrethroid insecticide permethrin and the azole fungicide propiconazole significantly increase mutation rates in E. coli, whereas A. baylyi and B. subtilis are not significantly affected. Moreover, we show that the carbamate IPBC and the insecticide permethrin affect HGT in a concentration dependent manner. Investigations with reporter strains for bacterial stress response pathways show that induction of the general stress response (rpoS) and components of the SOS response (recA) underlie the effects of most biocides on mutation rates and HGT. Taken together, our data is important for assessing the contribution of biocides on AMR evolution and spread in the environment. T2 - World Microbe Forum (ASM, FEMS) CY - Online meeting DA - 20.06.2021 KW - Biocides KW - Horizontal gene transfer KW - Mutation rate PY - 2021 UR - https://www.abstractsonline.com/pp8/#!/9286/presentation/11692 AN - OPUS4-54211 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, Selina T1 - Effect of biocides on the evolution and spread of resistance N2 - Biocides used as material preservatives are in contact with various environments during direct application or passive leaching from protected materials. Antimicrobial resistance (AMR) is a global health problem and the environment is an important compartment for the evolution and transmission of AMR. Soil is an environment with a large reservoir of natural microbial communities and antimicrobial resistance (AMR) genes. Those natural microbial communities are frequently exposed to biocides used as material preservatives. Previous studies have shown that antibiotics, metals and pesticides affect the underlying processes of resistance evolution and spread; namely de novo mutagenesis and horizontal gene transfer by conjugation and transformation in microbial communities. However, it is unknown if active substances used in material preservatives are involved in these processes. We show that biocides used as material preservatives affect rates of mutation and conjugation in microorganism in a species- and substance-dependent manner, while rates of transformation are not directly affected. Our data highlights the importance of assessing the contribution of material preservatives on AMR evolution and spread in the environment. T2 - Microbiome Network Meeting CY - Berlin, Germany DA - 20.07.2022 KW - Biocides KW - Antimicrobial resistance KW - Horizontal gene transfer KW - Mutation rate PY - 2022 AN - OPUS4-56426 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, Selina T1 - Effect of biocides on the evolution and spread of resistance N2 - Biocides used as material preservatives are in contact with various environments during direct application or passive leaching from protected materials [1]. Antimicrobial resistance (AMR) is a global health problem and the environment is an important compartment for the evolution and transmission of AMR [2,3]. Soil is an environment with a large reservoir of natural microbial communities and antimicrobial resistance (AMR) genes. Those natural microbial communities are frequently exposed to biocides used as material preservatives. Previous studies have shown that antibiotics, metals and pesticides affect the underlying processes of resistance evolution and spread; namely de novo mutagenesis and horizontal gene transfer by conjugation and transformation in microbial communities. However, it is unknown if active substances used in material preservatives are involved in these processes. We show that biocides used as material preservatives affect rates of mutation and conjugation in microorganism in a species- and substance-dependent manner, while rates of transformation are not directly affected. Our data highlights the importance of assessing the contribution of material preservatives on AMR evolution and spread in the environment. T2 - RokoCon2022 CY - Berlin, Germany DA - 29.09.2022 KW - Biocide KW - Antimicrobial resistance KW - Tolerance KW - Horizontal gene transfer KW - Mutation rate PY - 2022 AN - OPUS4-56431 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Biocide Resistance - Road to Risk Assessment N2 - This presentation details the current status of biocide resistance risk assessment and provides a roadmap for future activities. T2 - OECD, 6th Meeting of the Working Party on Biocides CY - Paris, France DA - 28.09.2022 KW - Antimicrobial resistance KW - Antimicrobial coating KW - Standardization KW - Biocides KW - Risk assessment PY - 2022 AN - OPUS4-56263 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Die Entstehung von antimikrobiellen Resistenzen durch die Verwendung von Bioziden N2 - Dieser Vortrag gibt einen Überblick über die BAM und die Aktivitäten im Bereich Biozidresistenz. T2 - Berliner Hochschule für Technik Studiengang Biotechnologie CY - Berlin, Germany DA - 05.05.2023 KW - Antimikrobielle Resistenz KW - Antmikrobielle Oberflächen KW - Standardisierung PY - 2023 AN - OPUS4-57859 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - How to regulate and assess resistance risks of co-selecting agents during application and in the environment N2 - This talk deals with the question on How to regulate and assess resistance risks of co-selecting agents during application and in the environment. It shows that there the overview of worldwide activities to regulate co-selecting agents is missing. Regulations for product authorization usually consider resistance in target organisms, but there is a risk of emergence of resistance from non-target organisms as well. Moreover, pollution effects on resistance development in the environment are not explicitly covered during product authorization and few risk assessment schemes and methods available. T2 - EDAR7 - Environmental Dimension of Antimicrobial Resistance Conference 2024 CY - Montreal, Canada DA - 26.05.2024 KW - Antimicrobial resistance KW - Bacteria KW - Standardization KW - Biocides PY - 2024 AN - OPUS4-61542 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Development of a laboratory method to assess resistance development of microorganisms to biocides – An update N2 - This presentation describes the development of a laboratory method to assess resistance development of microorganisms to biocides and antimicrobial surfaces. T2 - The International Biodeterioration Research Group (IBRG) autumn meeting 2022 CY - Online meeting DA - 11.10.2022 KW - Antimicrobial resistance KW - Antimicrobial coating KW - Standardization KW - Biocides KW - Risk assessment PY - 2022 AN - OPUS4-56264 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Development of a laboratory method to assess resistance development of microorganisms to biocides – An update N2 - This presentation describes our efforts at BAM towards the development of a laboratory method to assess resistance development of microorganisms to biocides. T2 - The International Biodeterioration Research Group (IBRG) spring meeting 2023 CY - Online meeting DA - 05.03.2023 KW - Antimicrobial resistance KW - Antimicrobial coating KW - Standardization KW - Biocides PY - 2023 AN - OPUS4-57858 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Development of a laboratory method to assess resistance development of microorganisms to biocides N2 - Bacteria are exposed to biocides through surface disinfection or by antimicrobial surfaces. These stressful environments provide a strong selective pressure for bacteria to adapt. Here, we describe the development of a laboratory method to assess adaption of bacteria by resistance development in response to surface disinfection and antimicrobial surfaces. T2 - OECD, 5th Meeting of the Working Party on Biocides CY - Online meeting DA - 26.05.2021 KW - Antimicrobial resistance KW - Antimicrobial coating KW - Standardization PY - 2021 AN - OPUS4-53163 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schreiber, Frank T1 - Development of a laboratory method to assess resistance development of microorganisms to biocides N2 - Bacteria are exposed to biocides through surface disinfection or by antimicrobial surfaces. These stressful environments provide a strong selective pressure for bacteria to adapt. Here, we describe the development of a laboratory method to assess adaption of bacteria by resistance development in response to surface disinfection and antimicrobial surfaces. T2 - The International Biodeterioration Research Group (IBRG) Spring Meeting 2021 CY - Online meeting DA - 28.06.2021 KW - Antimicrobial resistance KW - Antimicrobial coating KW - Standardization PY - 2021 AN - OPUS4-53164 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -