TY - CONF A1 - Paul, Martin T1 - Fast Detection of TNT at ppt Level by an Immunofluorometric Biosensor N2 - The mechanism of this system is based on kinetic competition. This biosensor consists of a monolithic glass column with a vast excess of immobilized hapten, which traps the fluorescently labeled antibody as long as no explosive is present. If the explosive 2,4,6-trinitrotoluene (TNT) is introduced some binding sites of the antibody will be blocked, which leads to an immediate breakthrough of the labeled protein. The fluorescence is detected by highly sensitive laser-induced fluorescence with a conventional CMOS camera. The system achieved limits of detection of approx.1 pM (1 ppt) of the fluorescent label and around 100 pM (20 ppt) of TNT. The total assay time is less than 8 minutes. A cross-reactivity test with 5000 pM solutions of pentaerythritol tetranitrate (PETN), 1,3,5-trinitroperhydro-1,3,5-triazine (RDX), and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) showed no cross reactivity. T2 - Doktorandenseminar 2020 des Arbeitskreis-Prozessanalytik CY - Online meeting DA - 21.09.2020 KW - TNT KW - Explosive KW - Antibody KW - Fluorescence KW - Biosensor KW - Affinity PY - 2020 AN - OPUS4-51314 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Paul, Martin T1 - Development of a high-sensitivity biosensor for the quasi-continuous detection of the explosive TNT and the illegal drug cocaine N2 - An immunofluorometric biosensor with laser-induced fluorescence detection was presented. For TNT an LOD of 60 ppb and for cocaine an LOD of 9 ppb was achieved. A swipe test was performed in less than 3 minutes. No significant cross-reactivity was observed. T2 - 15. Interdisziplinäres Doktorandenseminar des Arbeitskreises Prozessanalytik der GDCh CY - Online meeting DA - 06.09.2021 KW - PETN KW - HMX KW - RDX KW - Microfluidic mixing KW - Lab-on-a-chip KW - Affinity column KW - Monolithic material KW - Sintered glass KW - Dy-654 KW - Flowcell KW - Epifluorescence KW - CMOS camera KW - Diode laser KW - Antibody screening PY - 2021 AN - OPUS4-53329 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Tscheuschner, Georg T1 - MALDI-TOF MS Fingerprinting of Antibodies in Less Than One Hour N2 - Antibodies are the most used biomolecules in analytical research. Nevertheless, the sequence and structure information of antibodies is often limited, since manufacturers keep them secret or suppliers sell them under different names. This can make it difficult to reproduce even basic experiments performed in publications as the antibodies used might not be identifiable. To overcome these problems, we developed a simple and cheap method for antibody identification by MALDI-TOF-MS fingerprinting. This technique was used to generate a library of antibody fingerprints, which enables the identification and comparison of antibodies in short time. T2 - SALSA Make and Measure 2021 CY - Online meeting DA - 16.09.2021 KW - Hydrolysis KW - Acid KW - Trypsin KW - Digestion KW - Fragment KW - Peptides KW - Mass spectrometry KW - Database KW - Clones KW - Monoclonal antibodies PY - 2021 AN - OPUS4-53294 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Tannenberg, Robert T1 - Detection of Cardiac Troponin I with Chemiluminescence Biosensor N2 - Cardiovascular diseases are the number one cause of death worldwide and responsible for 3.9 million deaths in Europe and over 1.8 million deaths in the European Union (2017) [1]. Cardiac Troponin I (cTnI) is part of a triple protein complex and the most important biomarker for acute events such as heart attacks. Once a heart attack occurs, the blood stream within the heart is interrupted and further oxygen supply cannot be maintained. As a result, heart cells undergo apoptosis and their proteins will be released into the blood stream. Within the project CardioMet of the European Metrology Program of Research and Innovation (EMPIR) a biosensor for online-monitoring of acute myocardial infarction by detecting cTnI is being developed. T2 - SALSA Make and Measure 2021 CY - Online meeting DA - 16.09.2021 KW - Sensor KW - Luminol KW - ECL KW - Peroxidase KW - Monolith KW - Lab on a Chip KW - EMPIR KW - 18HTL10 KW - CardioMet KW - Horizon 2020 PY - 2021 AN - OPUS4-53306 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Gabler, Mariella T1 - Development of an affinity-based method for the site-selective synthesis of antibody-drug-conjugates N2 - For the site-selective synthesis of ADCs, a variety of obstacles must be overcome. Those include designing bifunctional affinity peptides with reasonably low 𝐾𝑑-values that couple to the mAb in a site-selective manner. These peptides should also include a functional group that links the payload to the mAb under mild conditions without adversely affecting it. The bioconjugation between peptide and antibody and the linker between peptide and payload must be stable and durable to provide safety when used for medical purposes. The usage of metals and organic solvents should be minimized. Within the project, new types of functionalized affinity peptides were designed, and their affinity towards the Fc-fragment was determined. KW - Antibody drug conjugate KW - ADC KW - Human antibody KW - Peptide KW - Linker KW - Toxin KW - Payload KW - Monomethyl Auristatin E KW - MMAE KW - DM1 KW - Click chemistry KW - Copper-catalyzed KW - SDS-PAGE KW - HPLC KW - Trastuzumab KW - Herceptin KW - SPR KW - MALDI-TOF-MS KW - Mertansine KW - Site-selective bioconjugation KW - Affinity PY - 2021 SP - 1 EP - 100 PB - Humboldt-Universität zu Berlin CY - Berlin AN - OPUS4-54519 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Fischer, Janina T1 - Suspensionsarray-Fluoreszenzimmunoassay zur Multiplexbestimmung von SARS-CoV-2-Antikörpern N2 - Übergeordnetes Ziel der Arbeit war die Entwicklung und Optimierung eines Suspensionsarray-Fluoreszenzimmunoassays (SAFIA) für die simultane Bestimmung verschiedener gegen SARS-CoV-2-Proteine gerichtete Antikörper mittels Multiplexdetektion in komplexen Matrices wie humanen Blutserumproben. KW - SARS-CoV-2 KW - Corona KW - COVID-19 KW - Coronavirus KW - Virus KW - Spike-Protein KW - Nucleocapsid-Protein KW - RBD KW - SAFIA KW - LFIA KW - ELISA KW - Partikel KW - Mutationen KW - Neutralisierende Antikörper KW - Durchflusszytometrie KW - ACE2 PY - 2022 SP - 1 EP - 99 PB - Humboldt-Universität zu Berlin CY - Berlin AN - OPUS4-54722 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Nerger, Tino T1 - Passive Smart Dust: A versatile low-cost sensor platform N2 - Beschichtete Zellulose Partikel als kolorimetrische passive Sensoren, die mittels optische Kamerasystem einer Drohne erfasst werden. Somit können Gefahrenstoffe, in ersten Versuchen z. B. Säuren aus sicherer Entfernung, kostengünstig und umweltverträglich detektiert werden. T2 - SMSI 2023 Sensor and Measurement Science International CY - Nuremberg, Germany DA - 08.05.2023 KW - Passive Smart Dust KW - Drone KW - Colorimetric sensor particles PY - 2023 AN - OPUS4-57526 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Nerger, Tino A1 - Neumann, Patrick P. A1 - Weller, Michael G. T1 - Passive Smart Dust a versatile low-cost sensor platform N2 - Coated cellulose particles as colourimetric passive sensors that are detected by the optical camera system of a drone. In this way, hazardous substances, e.g. acids, can be detected from a safe distance in a cost-effective and environmentally friendly way. N2 - Beschichtete Zellulose Partikel als kolorimetrische passive Sensoren, die mittels optische Kamerasystem einer Drohne erfasst werden. Somit können Gefahrenstoffe, in ersten Versuchen zB Säuren aus sicherer Entfernung, kostengünstig und umweltverträglich detektiert werden. T2 - SMSI 2023 Sensor and Measurement Science International CY - Nuremberg, Germany DA - 08.05.2023 KW - Passive Smart Dust KW - Drone KW - Colorimetric sensor particles KW - Remote detection PY - 2023 DO - https://doi.org/10.5162/SMSI2023/C5.3 SP - 1 EP - 2 AN - OPUS4-57527 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Nerger, Tino T1 - Passive Smart Dust zur Erkennung gefährlicher Stoffe N2 - Einsatz/Verteilung kostengünstiger und umweltfreundlicher Partikel als passive Sensoren und optische Detektion per Kamerasystem. Die einfache Modifikation chemischen Farbstoffe auf der Partikeloberfläche liefert eine optisch quantifizierbare Reaktion und Rückschlüsse auf die Zielsubstanzen. T2 - Lange Nacht der Wissenschaften @ BAM 2023 CY - Berlin, Germany DA - 17.06.2023 KW - Passive Smart Dust KW - Drohne KW - Kolorimetrische Sensoren KW - Cellulose PY - 2023 AN - OPUS4-58140 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Nerger, Tino A1 - Neumann, Patrick P. A1 - Weller, Michael G. T1 - Passive Smart Dust For Detection Of Hazardous Substances N2 - High demand for remote sensing of hazardous substances. Possible solution: Use of distributed, low cost, and environmentally safe particles as passive sensors that can be read out remotely Chemical intelligence on the particle surface can be easily modified Particles enable optically quantifiable response and inference of target substances (also no maintenance or power supply required T2 - 38th Danubia-Adria Symposium on Advances in Experimental Mechanics CY - Poros, Greece DA - 20.09.2022 KW - Smart Dust KW - Drone KW - Remote Detection KW - Colorchanging Particles PY - 2022 AN - OPUS4-56025 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -