TY - JOUR A1 - Anderhalten, L. A1 - Silva, R. V. A1 - Morr, A. A1 - Wang, S. A1 - Smorodchenko, A. A1 - Saatz, Jessica A1 - Traub, Heike A1 - Mueller, S. A1 - Boehm-Sturm, P. A1 - Rodriguez-Sillke, Y. A1 - Kunkel, D. A1 - Hahndorf, J. A1 - Paul, F. A1 - Taupitz, M. A1 - Sack, I. A1 - Infante-Duarte, C. T1 - Different Impact of Gadopentetate and Gadobutrol on Inflammation-Promoted Retention and Toxicity of Gadolinium Within the Mouse Brain N2 - Objectives: Using a murine model of multiple sclerosis, we previously showed that repeated administration of gadopentetate dimeglumine led to retention of gadolinium (Gd) within cerebellar structures and that this process was enhanced with inflammation. This study aimed to compare the kinetics and retention profiles of Gd in inflamed and healthy brains after application of the macrocyclic Gd-based contrast agent (GBCA) gadobutrol or the linear GBCA gadopentetate. Moreover, potential Gd-induced neurotoxicity was investigated in living hippocampal slices ex vivo. Materials and Methods: Mice at peak of experimental autoimmune encephalomyelitis (EAE; n = 29) and healthy control mice (HC; n = 24) were exposed to a cumulative dose of 20 mmol/kg bodyweight of either gadopentetate dimeglumine or gadobutrol (8 injections of 2.5 mmol/kg over 10 days). Magnetic resonance imaging (7 T) was performed at baseline as well as at day 1, 10, and 40 post final injection (pfi) of GBCAs. Mice were sacrificed after magnetic resonance imaging and brain and blood Gd content was assessed by laser ablation-inductively coupled plasma (ICP)-mass spectrometry (MS) and ICP-MS, respectively. In addition, using chronic organotypic hippocampal slice cultures, Gd-induced neurotoxicity was addressed in living brain tissue ex vivo, both under control or inflammatory (tumor necrosis factor α [TNF-α] at 50 ng/μL) conditions. Results: Neuroinflammation promoted a significant decrease in T1 relaxation times after multiple injections of both GBCAs as shown by quantitative T1 mapping of EAE brains compared with HC. This corresponded to higher Gd retention within the EAE brains at 1, 10, and 40 days pfi as determined by laser ablation-ICP-MS. In inflamed cerebellum, in particular in the deep cerebellar nuclei (CN), elevated Gd retention was observed until day 40 after last gadopentetate application (CN: EAE vs HC, 55.06 ± 0.16 μM vs 30.44 ± 4.43 μM). In contrast, gadobutrol application led to a rather diffuse Gd content in the inflamed brains, which strongly diminished until day 40 (CN: EAE vs HC, 0.38 ± 0.08 μM vs 0.17 ± 0.03 μM). The analysis of cytotoxic effects of both GBCAs using living brain tissue revealed an elevated cell death rate after incubation with gadopentetate but not gadobutrol at 50 mM. The cytotoxic effect due to gadopentetate increased in the presence of the inflammatory mediator TNF-α (with vs without TNF-α, 3.15% ± 1.18% vs 2.17% ± 1.14%; P = 0.0345). Conclusions: In the EAE model, neuroinflammation promoted increased Gd retention in the brain for both GBCAs. Whereas in the inflamed brains, efficient clearance of macrocyclic gadobutrol during the investigated time period was observed, the Gd retention after application of linear gadopentetate persisted over the entire observational period. Gadopentetate but not gadubutrol appeared to be neurotoxic in an ex vivo paradigm of neuronal inflammation. KW - Imaging KW - ICP-MS KW - Gadolinium KW - Contrast agent KW - Laser ablation KW - Brain KW - Multiple sclerosis PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-546910 DO - https://doi.org/10.1097/RLI.0000000000000884 SN - 0020-9996/22/0000–0000 VL - 57 IS - 10 SP - 677 EP - 688 PB - Wolters Kluwer N.V. CY - Alphen aan den Rijn, The Netherlands AN - OPUS4-54691 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kader, A. A1 - Brangsch, J. A1 - Reimann, C. A1 - Kaufmann, Jan Ole A1 - Mangarova, D. B. A1 - Moeckel, J. A1 - Adams, L. C. A1 - Zhao, J. A1 - Saatz, Jessica A1 - Traub, Heike A1 - Buchholz, R. A1 - Karst, U. A1 - Hamm, B. A1 - Makowski, M. R. T1 - Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe N2 - One of the most commonly diagnosed cancers in men is prostate cancer (PCa). Understanding tumor progression can help diagnose and treat the disease at an early stage. Components of the extracellular matrix (ECM) play a key role in the development and progression of PCa. Elastin is an essential component of the ECM and constantly changes during tumor development. This article visualizes and quantifies elastin in magnetic resonance imaging (MRI) using a small molecule probe. Results were correlated with histological examinations. Using an elastin-specific molecular probe, we were able to make predictions about the cellular structure in relation to elastin and thus draw conclusions about the size of the tumor, with smaller tumors having a higher elastin content than larger tumors. Human prostate cancer (PCa) is a type of malignancy and one of the most frequently diagnosed cancers in men. Elastin is an important component of the extracellular matrix and is involved in the structure and organization of prostate tissue. The present study examined prostate cancer in a xenograft mouse model using an elastin-specific molecular probe for magnetic resonance molecular imaging. Two different tumor sizes (500 mm3 and 1000 mm3) were compared and analyzed by MRI in vivo and histologically and analytically ex vivo. The T1-weighted sequence was used in a clinical 3-T scanner to calculate the relative contrast enhancement before and after probe administration. Our results show that the use of an elastin-specific probe enables better discrimination between tumors and surrounding healthy tissue. Furthermore, specific binding of the probe to elastin fibers was confirmed by histological examination and laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS). Smaller tumors showed significantly higher signal intensity (p > 0.001), which correlates with the higher proportion of elastin fibers in the histological evaluation than in larger tumors. A strong correlation was seen between relative enhancement (RE) and Elastica–van Gieson staining (R2 = 0.88). RE was related to inductively coupled plasma–mass spectrometry data for Gd and showed a correlation (R2 = 0.78). Thus, molecular MRI could become a novel quantitative tool for the early evaluation and detection of PCa. KW - Magnetic resonance imaging KW - MRI KW - Molecular imaging KW - Cancer KW - LA-ICP-MS PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-538410 DO - https://doi.org/10.3390/biology10111217 VL - 10 IS - 11 SP - 1 EP - 14 PB - MDPI CY - Basel AN - OPUS4-53841 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kaufmann, Jan Ole A1 - Brangsch, J. A1 - Kader, A. A1 - Saatz, Jessica A1 - Mangarova, D. B. A1 - Zacharias, M. A1 - Kempf, W. E. A1 - Schwaar, T. A1 - Ponader, Marco A1 - Adams, L. C. A1 - Möckel, J. A1 - Botnar, R. M. A1 - Taupitz, M. A1 - Mägdefessel, L. A1 - Traub, Heike A1 - Hamm, B. A1 - Weller, Michael G. A1 - Makowski, M. R. T1 - ADAMTS4-specific MR-probe to assess aortic aneurysms in vivo using synthetic peptide libraries N2 - The incidence of abdominal aortic aneurysms (AAAs) has substantially increased during the last 20 years and their rupture remains the third most common cause of sudden death in the cardiovascular field after myocardial infarction and stroke. The only established clinical parameter to assess AAAs is based on the aneurysm size. Novel biomarkers are needed to improve the assessment of the risk of rupture. ADAMTS4 (A Disintegrin And Metalloproteinase with ThromboSpondin motifs 4) is a strongly upregulated proteoglycan cleaving enzyme in the unstable course of AAAs. In the screening of a one-bead-one-compound library against ADAMTS4, a low-molecular-weight cyclic peptide is discovered with favorable properties for in vivo molecular magnetic resonance imaging applications. After identification and characterization, it’s potential is evaluated in an AAA mouse model. The ADAMTS4-specific probe enables the in vivo imaging-based prediction of aneurysm expansion and rupture. KW - Peptide KW - Peptide library KW - OBOC library KW - Combinatorial chemistry KW - Peptide aptamers KW - Binding molecule KW - Affinity KW - Synthetic peptides KW - Contrast agent KW - Magnetic resonance imaging KW - One-bead-one-compound library KW - On-chip screening KW - Lab-on-a-chip KW - MALDI-TOF MS KW - SPR KW - Surface plasmon resonance KW - Alanine scan KW - Fluorescence label KW - MST KW - Docking KW - Chelate PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-560930 DO - https://doi.org/10.1038/s41467-022-30464-8 N1 - Geburtsname von Ponader, Marco: Wilke, M. - Birth name of Ponader, Marco: Wilke, M. VL - 13 IS - 1 SP - 1 EP - 18 PB - Springer Nature Limited CY - Heidelberg AN - OPUS4-56093 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kader, A. A1 - Kaufmann, Jan Ole A1 - Mangarova, D. B. A1 - Moeckel, J. A1 - Brangsch, J. A1 - Adams, L. C. A1 - Zhao, J. A1 - Reimann, C. A1 - Saatz, Jessica A1 - Traub, Heike A1 - Buchholz, R. A1 - Karst, U. A1 - Hamm, B. A1 - Makowski, M. R. T1 - Iron Oxide Nanoparticles for Visualization of Prostate Cancer in MRI N2 - Prostate cancer (PCa) is one of the most common cancers in men. For detection and diagnosis of PCa, non-invasive methods, including magnetic resonance imaging (MRI), can reduce the risk potential of surgical intervention. To explore the molecular characteristics of the tumor, we investigated the applicability of ferumoxytol in PCa in a xenograft mouse model in two different tumor volumes, 500 mm3 and 1000 mm3. Macrophages play a key role in tumor progression, and they are able to internalize iron-oxide particles, such as ferumoxytol. When evaluating T2*-weighted sequences on MRI, a significant decrease of signal intensity between pre- and post-contrast images for each tumor volume (n = 14; p < 0.001) was measured. We, furthermore, observed a higher signal loss for a tumor volume of 500 mm3 than for 1000 mm3. These findings were confirmed by histological examinations and laser ablation inductively coupled plasma-mass spectrometry. The 500 mm3 tumors had 1.5% iron content (n = 14; sigma = 1.1), while the 1000 mm3 tumors contained only 0.4% iron (n = 14; sigma = 0.2). In vivo MRI data demonstrated a correlation with the ex vivo data (R2 = 0.75). The results of elemental analysis by inductively coupled plasma-mass spectrometry correlated strongly with the MRI data (R2 = 0.83) (n = 4). Due to its long retention time in the blood, biodegradability, and low toxicity to patients, ferumoxytol has great potential as a contrast agent for visualization PCa. KW - Imaging KW - Nanoparticle KW - Cancer KW - Iron oxide KW - ICP-MS KW - Magnetic resonance imaging PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-550075 DO - https://doi.org/10.3390/cancers14122909 VL - 14 IS - 12 SP - 1 EP - 13 PB - MDPI CY - Basel, Switzerland AN - OPUS4-55007 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Golusda, L. A1 - Kühl, A. A. A1 - Lehmann, M. A1 - Dahlke, K. A1 - Mueller, S. A1 - Boehm-Sturm, P. A1 - Saatz, Jessica A1 - Traub, Heike A1 - Schnorr, J. A1 - Freise, C. A1 - Taupitz, M. A1 - Biskup, K. A1 - Blanchard, V. A1 - Klein, O. A1 - Sack, I. A1 - Siegmund, B. A1 - Paclik, D. T1 - Visualization of inflammation in experimental colitis by magnetic resonance imaging using very small superparamagnetic iron oxide particles N2 - Inflammatory bowel diseases (IBD) comprise mainly ulcerative colitis (UC) and Crohn´s disease (CD). Both forms present with a chronic inflammation of the (gastro) intestinal tract, which induces excessive changes in the composition of the associated extracellular matrix (ECM). In UC, the inflammation is limited to the colon, whereas it can occur throughout the entire gastrointestinal tract in CD. Tools for early diagnosis of IBD are still very limited and highly invasive and measures for standardized evaluation of structural changes are scarce. To investigate an efficient non-invasive way of diagnosing intestinal inflammation and early changes of the ECM, very small superparamagnetic iron oxide nanoparticles (VSOPs) in magnetic resonance imaging (MRI) were applied in two mouse models of experimental colitis: the dextran sulfate sodium (DSS)-induced colitis and the transfer model of colitis. For further validation of ECM changes and inflammation, tissue sections were analyzed by immunohistochemistry. For in depth ex-vivo investigation of VSOPs localization within the tissue, Europium-doped VSOPs served to visualize the contrast agent by imaging mass cytometry (IMC). VSOPs accumulation in the inflamed colon wall of DSS-induced colitis mice was visualized in T2* weighted MRI scans. Components of the ECM, especially the hyaluronic acid content, were found to influence VSOPs binding. Using IMC, colocalization of VSOPs with macrophages and endothelial cells in colon tissue was shown. In contrast to the DSS model, colonic inflammation could not be visualized with VSOP-enhanced MRI in transfer colitis. VSOPs present a potential contrast agent for contrast-enhanced MRI to detect intestinal inflammation in mice at an early stage and in a less invasive manner depending on hyaluronic acid content. KW - Inflammation KW - Imaging KW - Immunohistochemistry KW - MRI KW - Nanoparticle KW - Extracellular matrix KW - Laser ablation KW - ICP-MS PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-555395 DO - https://doi.org/10.3389/fphys.2022.862212 SN - 1664-042X VL - 13 IS - July 2022 SP - 1 EP - 15 PB - Frontiers Research Foundation CY - Lausanne AN - OPUS4-55539 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hussein, S. A1 - Kühl, A. A. A1 - Golusda, L. A1 - Plattner, C. A1 - Heinze, N. A1 - Sturm, G. A1 - Freise, C. A1 - Traub, Heike A1 - Schannor, Mathias A1 - Trajanoski, Z. A1 - Taupitz, M. A1 - Siegmund, B. A1 - Paclik, D. T1 - Phenotype and function of human monocytes remain mainly unaffected by very small superparamagnetic iron oxide particles N2 - The field of medical application of organic or inorganic nanoparticles is extensive. Medical nanoparticles offer benefits but pose risks. For safe use in diagnostics and therapy, they should be inert, non-immunogenic, non-aggregating, and avoid long-term accumulation in sensitive tissues like bone marrow or the brain. We have developed in-house very small superparamagnetic iron oxide nanoparticles (VSOP), 7 nm in size, which have been successfully used in preclinical magnetic resonance imaging (MRI) to detect intestinal inflammation, neuroinflammation and atherosclerosis. This study examines nanoparticle effects on human blood cells focusing on monocytes in vitro as a first step toward clinical application. Whole blood and monocytes from healthy donors and patients with inflammatory bowel disease were treated with VSOP in vitro and analyzed for changes in their transcriptome, phenotype and function. RNA sequencing of monocytes identified the transferrin receptor as one of the most significantly downregulated genes after VSOP treatment, likely to limit iron uptake. Whereas whole blood RNA sequencing showed significant changes only in three non-coding genes. CyTOF analysis confirmed that VSOP-treated monocytes remain inactive, with no increased proliferation or altered migration. Metabolically, VSOP uptake enhanced the oxygen consumption rate. This effect was likely due to phagocytosis rather than effects mediated by the VSOP itself, as phagocytosis of latex beads showed comparable results. In summary, the analysis of peripheral blood mononuclear cells and monocytes suggests that VSOP treatment has no major impact on immune cell phenotype or function indicating VSOP as a promising diagnostic tool in MRI for inflammatory bowel disease. KW - Imaging KW - Nanoparticle KW - Monocytes KW - Contrast agent KW - Diagnostics KW - ICP-MS KW - LA-ICP-MS KW - VSOP PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-632012 DO - https://doi.org/10.3389/fnano.2025.1584000 SN - 2673-3013 VL - 7 SP - 1 EP - 16 PB - Frontiers Media CY - Lausanne AN - OPUS4-63201 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Scharek, Vera M. A1 - Pfeifer, Jens A1 - Vogl, Jochen A1 - Traub, Heike A1 - Meermann, Björn T1 - Tin fractionation analysis in sediment samples via on-line ID ETV/ICP-MS N2 - We report a quantification approach for directly determining total tin and tin-based pollutants/species in sediments via electrothermal vaporization/inductively coupled plasma-mass spectrometry (ETV/ICP-MS) utilizing an on-line isotope dilution mass spectrometry (IDMS) approach. The method was developed and validated using an estuarine sediment reference material (BCR-277R), yielding a recovery of 106%. A relative standard deviation (RSD) of 14%, comparable to published data using a similar method, was obtained. A limit of quantification (LOQ) was estimated at 0.008 mg Sn kg−1 and sufficient for quantifying the total tin mass fraction of surface sediments along the tidal River Elbe course. Hereby, a decrease towards the river mouth, presumably due to dilution effects by less polluted marine sediment, was observed. Besides total tin, monitoring of organotin compounds (OTCs)/species is of interest in sediments due to their toxic effects on aquatic life. The method’s capability was extended by separating an OTC fraction in a sediment certified reference material (CRM) through the ETV temperature program. While spiking experiments with OTC standards confirmed the assignment, only a small fraction of the total certified OTC amount (3%), likely due to matrix effects, was recovered. However, applying the method to real-world samples, OTCs were detectable along the River Elbe course. By this, we demonstrated the potential of our method as a complementary fast-screening approach to species-specific analysis procedures. KW - ETV KW - Sn-Speciation KW - Sediment PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-640137 DO - https://doi.org/10.1007/s00216-025-06064-y SN - 1618-2642 SP - 1 EP - 10 PB - Springer Science and Business Media LLC AN - OPUS4-64013 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, D. A1 - Büchner, T. A1 - Schrade, P. A1 - Traub, Heike A1 - Werner, S. A1 - Guttmann, P. A1 - Bachmann, S. A1 - Kneipp, J. T1 - Influence of Nuclear Localization Sequences on the Intracellular Fate of Gold Nanoparticles N2 - Directing nanoparticles to the nucleus by attachment of nuclear localization sequences (NLS) is an aim in many applications. Gold nanoparticles modified with two different NLS were studied while crossing barriers of intact cells, including uptake, endosomal escape, and nuclear translocation. By imaging of the nanoparticles and by characterization of their molecular interactions with surface-enhanced Raman scattering (SERS), it is shown that nuclear translocation strongly depends on the particular incubation conditions. After an 1 h of incubation followed by a 24 h chase time, 14 nm gold particles carrying an adenoviral NLS are localized in endosomes, in the cytoplasm, and in the nucleus of fibroblast cells. In contrast, the cells display no nanoparticles in the cytoplasm or nucleus when continuously incubated with the nanoparticles for 24 h. The ultrastructural and spectroscopic data indicate different processing of NLS-functionalized particles in endosomes compared to unmodified particles. NLS functionalized nanoparticles form larger intraendosomal aggregates than unmodified gold nanoparticles. SERS spectra of cells with NLS-functionalized gold nanoparticles contain bands assigned to DNA and were clearly different from those with unmodified gold nanoparticles. The different processing in the presence of an NLS is influenced by a continuous exposure of the cells to nanoparticles and an ongoing nanoparticle uptake. This is supported by mass-spectrometry-based quantification that indicates enhanced uptake of NLS-functionalized nanoparticles compared to unmodified particles under the same conditions. The results contribute to the optimization of nanoparticle analysis in cells in a variety of applications, e.g., in theranostics, biotechnology, and bioanalytics. KW - Nanoparticle KW - Laser ablation KW - SERS KW - ICP-MS PY - 2021 DO - https://doi.org/10.1021/acsnano.1c04925 SN - 1936-086X VL - 15 IS - 9 SP - 14838 EP - 14849 PB - American Chemical Society AN - OPUS4-54047 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schannor, Mathias A1 - Oelze, Marcus A1 - Traub, Heike A1 - He, Yubei A1 - Schmidt, Robin A1 - Heidemann, Luisa A1 - Savic, Lynn Jeanette A1 - Vogl, Jochen A1 - Meermann, Björn T1 - Advancing Biomarker Research: In Situ Cu Isotope Analysis in Liver Tumors by LA-MC-ICP-MS N2 - Stable metal isotopes have received increasing attention as medical biomarkers due to their potential to detect changes in metal metabolism related to diseases. In particular, copper stable isotopes are a powerful tool to identify isotopic variation between tumors and healthy tissue, suggesting application in cancer diagnosis. However, potential mechanisms causing isotope fractionation, such as redox- or bond-forming reactions and interactions of metals during transmembrane import and export, are less well understood. Here, we established an in situ method using laser ablation-multicollector-inductively coupled plasma-mass spectrometry (LA-MC-ICP-MS) to advance our understanding of the underlying processes responsible for isotope fractionation between normal and diseased tissues. Gelatin-based bracketing standards and quality control reference materials, crucial for laser ablation analysis, were developed to allow correction for instrumentally induced isotope fractionation during LA-MC-ICP-MS analysis. Using such matrix-matched standards, the method achieved intermediate precisions for delta values of better than 0.15 ‰ (2 s) for inorganic reference materials and of better than 0.17 ‰ (2 s) for biological reference materials. The developed routine was tested on rabbit VX2 liver tumor samples, a model system resembling human hepatocellular carcinoma (HCC) used to study liver cancer. In situ Cu isotope compositions between healthy (𝛿65/63NIST976(Cu) = −1.5 ‰ to 0.2 ‰) and tumorous (𝛿65/63NIST976(Cu) = 0.0 ‰ to 1.3 ‰) liver tissue show distinct differences in their isotope ratios. The observed isotopic dichotomy is consistent with previous solution-based MC-ICP-MS work, showing enrichment of heavy 65Cu in cancer biopsies relative to healthy tissue. KW - Biomarker KW - Laser Ablation KW - Reference Material PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-626865 DO - https://doi.org/10.1021/acs.analchem.4c05626 SN - 1520-6882 VL - 97 IS - 8 SP - 4425 EP - 4432 PB - American Chemical Society (ACS) AN - OPUS4-62686 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Löwa, N. A1 - Golusda, L. A1 - Paclik, D. A1 - Traub, Heike A1 - Schannor, Mathias A1 - Saatz, Jessica A1 - Freise, C. A1 - Taupitz, M. A1 - Siegmund, B. A1 - Kühl, A. A. A1 - Wiekhorst, F. T1 - Magnetic particle spectroscopy for Eu-VSOP quantification in intestinal inflammation: Distinguishing nanoparticle signals from dietary contamination N2 - Magnetic nanoparticles are gaining increasing attention as a promising alternative to gadolinium-based contrast agents in magnetic resonance imaging, primarily due to their low toxicity. In this study, we investigated the use of magnetic iron oxide nanoparticles in mouse models of intestinal inflammation to assess their potential for detecting changes in the extracellular matrix. For magnetic quantification, we employed magnetic particle spectroscopy, which offers high sensitivity and minimal interference from biological tissue. However, we observed significant variations in magnetic signals within the intestine, as well as measurable signals in control animals, indicating possible magnetic contamination. By doping the nanoparticles with europium, we were able to confirm this suspicion through quantitative elemental analysis. Examination of mouse feed and feces allowed us to identify the source of contamination. Based on these findings, we developed a method to reliably distinguish genuine signals of magnetic nanoparticles from those caused by external magnetic contaminations. This approach is essential to ensure reliable results in future diagnostic and preclinical research. KW - ICP-MS KW - Nanoparticle KW - Magnetic particle spectroscopy KW - Quantification PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-642238 DO - https://doi.org/10.1039/D5NA00452G SN - 2516-0230 IS - 00452 SP - 1 EP - 10 PB - Royal Society of Chemistry (RSC) CY - Cambridge AN - OPUS4-64223 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Traub, Heike ED - Milacic, R. ED - Scancar, J. ED - Goenaga-Infante, H. ED - Vidmar, J. T1 - Imaging of metal-based nanoparticles in tissue and cell samples by laser ablation inductively coupled plasma mass spectrometry N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is nowadays a versatile and powerful analytical method for direct solid sample analysis. The applicability has been demonstrated for a wide variety of samples covering hard and soft materials. In an imaging mode the technique provides quantitative information on the elemental distribution within a sample. LA-ICP-MS imaging is of particular interest in biomedical research as the distribution of an element gives valuable insight on uptake and distribution of essential and toxic trace elements, administered contrast agents as well es nanoparticles. LA-ICP-MS is therefore a powerful complement to other imaging techniques. Recent instrumental improvements, especially in sample chamber design, have contributed to better sensitivity and spatial resolution enabling subcellular imaging. The book chapter provides a comprehensive overview about spatially resolved localisation and quantification of various nanoparticles in cells and tissue thin sections by LA-ICP-MS. Furthermore, different sample preparation strategies and internal standardisation and calibration approaches for bioimaging by LA-ICP-MS are summarized and discussed. Metal-containing nanomaterials are used in numerous fields ranging from industrial applications to nanomedicine. Several studies have demonstrated that the physicochemical properties of nanoparticles have an impact on their pharmacokinetics, transfer and clearance. The high sensitivity and multielement capability of LA-ICP-MS enables the elucidation of interactions between tissue components and nanomaterials used as imaging probes or drug carriers. Potential toxic effects are investigated as well. Thus, LA imaging significantly supports the clinical translation of safe and efficient nanoparticles for diagnostic and therapeutic purposes. KW - Laser ablation KW - ICP-MS KW - Imaging KW - Nanoparticle KW - Nanomaterial KW - Tissue KW - Cell PY - 2021 SN - 978-0-323-85305-7 SN - 0166-526X VL - 93 SP - 173 EP - 240 PB - Elsevier CY - Amsterdam ET - 1 AN - OPUS4-52775 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Blanchard, V. A1 - Traub, Heike T1 - Central project for biochemical analysis of proteoglycans and glycosaminoglycans and for element-specific microscopy N2 - Nearly all disease processes are associated with variations of components of the extracellular matrix (ECM) that are typically observed during the development of inflammation. This concerns for example proteoglycans and their associated glycosaminoglycans (GAG), which have been shown to bind to cationic metal imaging probes due to their strong complexing activity. The complexing activity largely depends on the degree of GAG sulfation and/or carboxylation as well as on the GAG isomericity. In this central project, we investigate GAG structures from inflammatory disorders (namely cardiovascular diseases, inflammatory intestinal diseases and neuroinflammation) provided by researchers of the Collaborative Research Center at the molecular disaccharidic level using chromatographic and mass spectrometric methods. In parallel, the spatial localization and quantification of metal-based imaging probes are evaluated by LA-ICP-MS imaging. T2 - 1st International Symposium In vivo Visualization of Extracellular Matrix Pathology CY - Online Meeting DA - 27.05.2021 KW - Laser ablation KW - ICP-MS KW - MALDI PY - 2021 AN - OPUS4-52716 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Phukphatthanachai, P. A1 - Panne, Ulrich A1 - Traub, Heike A1 - Pfeifer, Jens A1 - Vogl, Jochen T1 - Quantification of sulphur in copper and copper alloys by GDMS and LA-ICP-MS, demonstrating metrological traceability to the international system of units N2 - The quantification of the sulphur mass fraction in pure copper and copper alloys by GDMS and LA-ICP-MS revealed a lack of traceability mainly due to a lack of suitable certified reference materials for calibrating the instruments. Within this study GDMS and LA-ICP-MS were applied as routine analytical tools to quantify sulphur in copper samples by applying reference materials as calibrators, which were characterized for their sulphur mass fraction by IDMS beforehand. Different external calibration strategies were applied including a matrix cross type calibration. Both techniques with all calibration strategies were validated by using certified reference materials (others than those used for calibration) and good agreement with the reference values was achieved except for the matrix cross type calibration, for which the agreement was slightly worse. All measurement results were accompanied by an uncertainty statement. For GDMS, the relative expanded (k = 2) measurement uncertainty ranged from 3% to 7%, while for LA-ICP-MS it ranged from 11% to 33% when applying matrix-matched calibration in the sulphur mass fraction range between 25 mg kg-1 and 1300 mg kg-1. For cross-type calibration the relative expanded (k = 2) measurement uncertainty need to be increased to at least 12% for GDMS and to at least 54% for LA-ICP-MS to yield metrological compatibility with the reference values. The so obtained measurement results are traceable to the international system of units (SI) via IDMS reference values, which is clearly illustrated by the unbroken chain of calibrations in the metrological traceability scheme. KW - Sulfur KW - Copper KW - GDMS KW - LA-ICP-MS KW - Uncertainty KW - Traceability KW - SI PY - 2021 DO - https://doi.org/10.1039/d1ja00137j SN - 0267-9477 VL - 36 IS - 11 SP - 2404 EP - 2414 PB - Royal Society of Chemistry AN - OPUS4-53412 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Elemental mapping to study the interaction of MRI contrast agents with extracellular matrix components N2 - Non-specific Gd-based contrast agents (GBCAs) have long been routinely used in clinical magnetic resonance imaging (MRI) diagnostics. Nevertheless, the interaction of such contrast agents with tissue components is not yet fully understood. Typically, they go into extracellular space. The extracellular matrix (ECM) is a three-dimensional network of macromolecules providing structural and biochemical support of the surrounding cells in all mammalian tissues. It is composed of structural proteins (e.g., collagen, elastin) and proteoglycans, which consist of glycosaminoglycans (GAGs) covalently bound to a protein core. GAGs are long, linear polysaccharides composed of repeating disaccharide units that differ in molecular mass, disaccharide structure and degree of sulfation. Many diseases, including inflammation, fibrosis, and tumor invasion, are associated with characteristic ECM changes, especially at an early stage of disease development. As disease severity increases, the amount of one or more different GAG types in the ECM and the extent of GAG sulfation increases. Characteristic of GAGs is their ability to form complexes with cations, e.g., with lanthanides. Thus, GAGs could be a potential binding partner for GBCA molecules as a whole or for dechelated Gd. Currently, there are still many unanswered questions about the interaction of contrast agents with ECM components. In this study, therefore, the uptake and distribution of ionic Gd and various linear and macrocyclic GBCAs was investigated in spheroids as model systems mimicking the complex physiologically relevant tissue microenvironment. Chinese hamster ovary (CHO) cells and CRL-2242 cells, a CHO mutant that does not produce sulfated GAGs, were used to prepare spheroids. Afterwards the spheroids were incubated with gadolinium chloride and GBCAs. For elemental mapping laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) and synchrotron radiation nano X-ray fluorescence spectroscopy (syn-nanoXRF) were used. Although all spheroids were exposed to identical Gd concentrations, differences were observed in the spatial distribution and the amount of Gd taken up. After incubation with linear and macrocyclic GBCAs, Gd is detected in the interior of both types of spheroids. Furthermore, differences in the Gd amount were found depending on the GBCA used. In contrast, incubation with gadolinium chloride leads to an Gd enrichment in the outer regions of the spheroids as well as to much higher Gd contents compared to incubation with GBCAs. Both LA-ICP-TOFMS and syn-nanoXRF can make an important contribution to better understand the relationship between the affinity of GBCAs and ECM components. However, to elucidate such complex interactions, further studies are needed, also with other (bio-)analytical techniques. T2 - Euroanalysis 2025 CY - Barcelona, Spain DA - 31.08.2025 KW - ICP-MS KW - Laser ablation KW - Contrast agent KW - Spheroid KW - Extracellular matrix PY - 2025 AN - OPUS4-64143 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Theiner, S. A1 - Corte Rodriguez, M. A1 - Traub, Heike ED - Golloch, A. T1 - Novel applications of lanthanoids as analytical or diagnostic tools in the life sciences by ICP-MS based techniques N2 - Inductively coupled plasma-mass spectrometry (ICP-MS) is a well-established analytical method offering high sensitivity and multi-element analysis. ICP-MS has found acceptance in various application areas ranging from material analysis to applications in the life sciences. Within the last 15 years new strategies for the sensitive detection and accurate quantification of biomolecules in complex biomedical samples have been developed. Recent instrumental improvements have contributed to this progress. As most of the biomolecules do not contain endogenous metals etectable with ICP-MS, bioconjugation with artificial metal-containing tags based on metal-loaded chelate complexes or nanoparticles is increasingly applied to determine biomolecules indirectly. Especially, the combination of immunohistochemical workflows using lanthanoid-tagged antibodies and ICP-MS detection provides new insights in the complexity and interdependency of cellular processes. Single-cell ICP-MS, also termed as mass cytometry, allows high-dimensional analysis of biomarkers in cell populations at single-cell resolution. For that purpose, lanthanoid isotope labelled antibodies are used to detect their corresponding target molecules. The visualisation of the elemental distribution is possible with laser ablation ICP-MS (LA-ICPMS) at high spatial resolution. Especially, the combination of LA with ICP time-of-flight mass spectrometry, also referred to as imaging mass cytometry (IMC), opens new possibilities for multiparametric tissue imaging at the single-cell level and even below. The lanthanoid localisation and concentration can be linked to their conjugated antibody target providing valuable information about surface markers, intracellular signalling molecules to measure biological function, and the network state of an individual cell in a tissue. This book chapter focuses on new applications, where the multi-element capabilities of ICP-MS are used for the detection of lanthanoids applied as artificial elemental stains or tags for biomolecules and in particular antibodies. KW - ICP-MS KW - Laser ablation KW - Cell KW - Antibody KW - Immunohistochemistry KW - Lanthanoid KW - Mass cytometry KW - Imaging PY - 2022 SN - 978-3-11069-645-5 SN - 978-3-11069-636-3 DO - https://doi.org/10.1515/9783110696455-013 SP - 399 EP - 444 PB - De Gruyter CY - Berlin, Boston ET - 2. rev. and exten. edition AN - OPUS4-55118 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Püschel, S. A1 - Liestmann, Z. A1 - Kalusniak, S. A1 - Kränkel, C. A1 - Schulz, Andreas A1 - Traub, Heike A1 - Tanaka, H. T1 - Impact of Ho3+, Er3+, and Tm3+ on laser cooling of Yb:YLF N2 - Ytterbium-doped LiYF4 (Yb:YLF) is a promising material for all-solid-state optical cryocoolers, but the impact of foreign rare-earth impurities on the laser-cooling performance is not completely understood. In particular, Tm3+ has been reported to reduce the background absorption. This study quantitatively assesses the impact of Ho3+, Tm3+, and Er3+ impurities on laser-cooling of Yb:YLF by anti-Stokes fluorescence. We grew five Yb(5%):YLF crystals intentionally doped with tens of ppm levels of these impurities. Laser-induced thermal modulation spectroscopy tests confirmed that these rare-earth impurities reduce the external quantum efficiency of Yb:YLF without affecting the background absorption coefficient. Although Er3+ is a well-known quencher for Yb3+, Er3+ co-doping only slightly decreases the laser-cooling efficiency at low pump intensities but becomes detrimental at high pump intensities (>5 kW cm−2). However, this detrimental effect diminishes at lower temperatures, as evidenced by cooling an Er3+ co-doped crystal to the same minimum temperature of 144K as a solely Yb3+-doped crystal. Contrary to previous reports, Tm3+ proved to be the most detrimental among the three impurities. KW - ICP KW - Laser cooling KW - Impurity KW - LiYF4 KW - Crystal PY - 2024 DO - https://doi.org/10.1364/OME.538215 VL - 14 IS - 10 SP - 2334 EP - 2348 PB - Optica Publishing Group CY - Washington DC, USA AN - OPUS4-61046 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Scharek, Vera M. A1 - Kröger, Tommy A1 - Keil, Karin A1 - Traub, Heike A1 - Meermann, Björn T1 - A new elemental analytical approach for microplastic sum parameter analysis—ETV/ICP-MS with CO2 N2 - Microplastics (MPs) are pervasive environmental pollutants and are considered one of the main challenges of our time. However, a fast and comprehensive analytical approach for MP analysis in complex matrices traceable to SI units is still lacking. In this context, we report a fast screening tool for the sum parameter analysis of MPs using electrothermal vaporization (ETV) coupled to inductively coupled plasma-mass spectrometry (ICP-MS). In our proof-of-concept study, we observed size-independent detection of MPs as peaks above the 13C+ signal background in the nano- to micrometer range without limitations regarding the polymer type. Quantification of the 13C+ MP signals was accomplished via an external gas calibration utilizing dynamic dilution of carbon dioxide with argon, yielding recovery rates of 80–96% for MP reference material (RM) of polymer types commonly found in the environment. The applicability to a soil sample was demonstrated through spiking experiments with a polyethylene (PE) MP RM in soil. The limit of detection (LOD) was estimated to be 0.13 µg C, equaling the detection of a single spherical low-density-PE particle of about 70 µm, and a limit of quantification (LOQ) of 0.42 µg C. KW - ETV/ICP-MS KW - Mikroplastik KW - Summenparameter PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-646418 DO - https://doi.org/10.1007/s00216-025-06146-x SN - 1618-2642 SP - 1 EP - 10 PB - Springer CY - Berlin ; Heidelberg AN - OPUS4-64641 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bayerl, C. A1 - Shahryari, M. A1 - Reiter, R. A1 - Proß, V. A1 - Lehmann, K. A1 - Kühl, A. A. A1 - Becker, Dorit A1 - Schulz, Andreas A1 - Infante Duarte, C. A1 - Taupitz, M. A1 - Geisel, D. A1 - Tzschätzsch, H. A1 - Saatz, Jessica A1 - Traub, Heike A1 - Asbach, P. T1 - Quantitative Analysis of Gadolinium Deposits in Liver Tissue of Patients After Single or Multiple Gadolinium-based Contrast Agent Application N2 - Gadolinium-based contrast agents (GBCAs) are widely used in magnetic resonance imaging. Concerns exist regarding gadolinium deposition and its potential histopathologic tissue alterations, especially after repeated administrations of linear, less stable GBCAs. This study aimed to quantify gadolinium mass fractions in liver specimens of subjects exposed to GBCAs in correlation with histopathologic features. In this study, mass fractions of gadolinium in human liver specimens from 25 subjects who underwent liver tumor resection surgery and had received GBCA (1 to 9 times over 4 years), were quantitatively analyzed using inductively coupled plasma–mass spectrometry (ICP-MS). Histomorphology was assessed based on the nonalcoholic fatty liver disease activity score (NAS). Our results suggest that after intravenous administration of GBCA, a small fraction of gadolinium is retained in the liver over a time period of at least several weeks. A relationship was observed between Gadolinium retention and the number of GBCA administrations, but not with the cumulative dose and the degree of fatty liver disease. KW - ICP-MS KW - Contrast agent KW - Gadolinium KW - Liver PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-646608 DO - https://doi.org/10.1097/RLI.0000000000001254 SN - 1536-0210 SP - 1 EP - 10 PB - Lippincott Williams & Wilkins CY - Philadelphia, Pa. AN - OPUS4-64660 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Boyraz, B. A1 - Saatz, Jessica A1 - Pompös, I.-M. A1 - Gad, Michel A1 - Dernedde, J. A1 - Maier, A.-M. B. A1 - Moscovitz, O. A1 - Seeberger, P. H. A1 - Traub, Heike A1 - Tauber, R. T1 - Imaging Keratan Sulfate in Ocular Tissue Sections by Immunofluorescence Microscopy and LA-ICP-MS N2 - Carbohydrate-specific antibodies can serve as valuable tools to monitor alterations in the extracellular matrix resulting from pathologies. Here, the keratan sulfate-specific monoclonal antibody MZ15 was characterized in more detail by immunofluorescence microscopy as well as laser ablation ICP-MS using tissue cryosections and paraffin-embedded samples. Pretreatment with keratanase II prevented staining of samples and therefore demonstrated efficient enzymatic keratan sulfate degradation. Random fluorescent labeling and site-directed introduction of a metal cage into MZ15 were successful and allowed for a highly sensitive detection of the keratan sulfate landscape in the corneal stroma from rats and human tissue. KW - Laser ablation KW - Imaging KW - Glycosaminoglycan KW - ICP-MS KW - Immunohistochemistry KW - Immunofluorescence KW - Tissue PY - 2022 DO - https://doi.org/10.1021/acsabm.1c01240 VL - 5 IS - 2 SP - 853 EP - 861 PB - American Chemical Society CY - Washington AN - OPUS4-54341 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Unraveling the interaction of MRI contrast agents with tissue using LA ICP MS N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is increasingly used to study the distribution of metal-containing drugs, imaging probes and nanomaterials in connection with disease related changes and therapy progress. Additionally, biomolecules can be detected indirectly by using metal-tagged antibodies. The extracellular matrix (ECM) is, besides the cells, an important component of all body tissues. The macromolecular network of the ECM consists of structural proteins (e.g., collagen, elastin) and proteoglycans composed of highly negatively charged carbohydrates, the glycosaminoglycans (GAGs), which are covalently linked to a protein core. Many diseases, including inflammatory processes and tumors, are associated with characteristic ECM changes at an early stage. Recent studies have shown that contrast agents for magnetic resonance imaging (MRI), which are based on gadolinium containing chelate complexes or iron oxide nanoparticles, can bind themselves to ECM components. To elucidate the role of GAGs like keratan sulfate (KS) and its modification state in disease, highly specific tools are necessary. As a complement to conventional immunohistochemistry LA-ICP-MS was applied to investigate the distribution of KS in tissue thin sections using a well characterized anti-KS antibody labelled with metal ions. Furthermore, LA-ICP-MS was used for the detection of MRI contrast agents and the identification of their target cells and molecules in tissue samples from animal models, e.g. for cardiovascular diseases. The results show the possibilities of LA-ICP-MS for the elucidation of pathological tissue changes. T2 - European Workshop on Laser Ablation (EWLA 2022) CY - Berne, Switzerland DA - 12.07.2022 KW - Laser ablation KW - Imaging KW - ICP-MS KW - Antibody PY - 2022 AN - OPUS4-55315 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Laser Ablation ICP-ToF-MS to investigate the interaction of MRI contrast agents with extracellular matrix components N2 - Non-specific Gd-based contrast agents (GBCAs) have long been routinely used in clinical magnetic resonance imaging (MRI). However, the interaction of such contrast agents with tissue components is not yet fully understood. Alongside cells, the extracellular matrix (ECM) is an important component of mammalian tissue. It is a three-dimensional network of macromolecules that provides structural and biochemical support to the surrounding cells. Many diseases, including inflammation, fibrosis, and tumour invasion, are associated with characteristic ECM changes. The ECM consists of structural proteins (e.g., collagen, elastin) and proteoglycans, which are composed of glycosaminoglycans (GAGs) covalently linked to a protein core. GAGs are long, linear polysaccharides consisting of repeated disaccharide units widely varying in molecular mass, disaccharide structure, and sulfation degree. Characteristic of GAGs is their ability to form complexes with cations such as lanthanides. Thus, GAGs could be a potential binding partner for GBCA molecules as a whole or for dechelated Gd. Currently, there are still many unanswered questions about the interaction of contrast agents with ECM components. This study therefore investigated the uptake and distribution of different GBCAs in spheroids that mimic biological tissue and have different ECM expressions. Chinese hamster ovary (CHO) cells and GAG-depleted CRL-2242 cells were used to prepare spheroids. These were then incubated for several minutes with gadolinium chloride and various linear and macrocyclic GBCAs. Cryosections of the spheroids were used for imaging measurements with a low-dispersion laser ablation system coupled to an inductively coupled plasma time-of-flight mass spectrometer (LA/ICP-ToF-MS). Gelatine doped with multi-element solutions was used for matrix-matched quantification of Gd and other relevant elements such as Cu, Fe and Zn. Although all spheroids were incubated with identical Gd concentrations, significant differences in the amount of Gd taken up were observed. Gadolinium chloride is absorbed more strongly than the contrast agents and accumulates mainly in the outer regions of the spheroids. In contrast, after incubation with the linear and macrocyclic contrast agents, Gd is detected in the interior of both types of spheroids. T2 - European Workshop on Laser Ablation, EWLA 2024 CY - Ghent, Belgium DA - 02.07.2024 KW - ICP-MS KW - Laser ablation KW - Spheroid KW - Contrast agent PY - 2024 AN - OPUS4-60575 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - LA-ICP-TOFMS to study the interaction of MRI contrast agents with extracellular matrix components N2 - Non-specific Gd-based contrast agents (GBCAs) have been used in clinical magnetic resonance imaging (MRI) for more than 35 years. However, the interaction of such contrast agents with tissue components is not yet fully understood. Many diseases are associated with characteristic changes of the extracellular matrix (ECM). The ECM is a three-dimensional network of macromolecules providing structural and biochemical support of the surrounding cells in all mammalian tissues. It is composed of structural proteins (e.g., collagen, elastin) and proteoglycans, which consist of glycosaminoglycans (GAGs) covalently bound to a protein core. GAGs are long, linear polysaccharides composed of repeating disaccharide units that differ in molecular mass, disaccharide structure and degree of sulfation. GAGs are also characterized by their ability to form complexes with cations such as lanthanides. Thus, GAGs could be a potential binding partner for GBCAs as a whole or for dechelated Gd. Laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) was used to investigate the uptake and distribution of ionic Gd and various linear and macrocyclic GBCAs in spheroids mimicking biological tissue and exhibiting different ECM expressions. In addition to Gd, other relevant elements such as Cu, Fe, P and Zn were also monitored. Spheroids from Chinese hamster ovary (CHO) cells and GAG-depleted CRL-2242 cells were incubated with gadolinium chloride and various GBCAs. Although all spheroids were exposed to identical Gd concentrations, differences were observed in the spatial distribution and the amount of Gd taken up. After incubation with linear and macrocyclic GBCAs, Gd is detected in the interior of both types of spheroids. In contrast, incubation with gadolinium chloride leads to an enrichment in the outer regions of the spheroids as well as to much higher Gd contents compared to incubation with GBCAs. LA-ICP-TOFMS can make an important contribution to better understand the relationship between the affinity of GBCAs and ECM components such as GAGs. However, to elucidate such complex interactions, further studies are needed, also with other (bio-)analytical techniques. T2 - 20th European Winter Conference on Plasma Spectrochemistry (EWCPS-2025) CY - Berlin, Germany DA - 02.03.2025 KW - ICP-MS KW - Laser ablation KW - Contrast agent KW - Spheroid KW - Extracellular matrix PY - 2025 AN - OPUS4-62692 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - LA-ICP-MS investigation of the the interaction of MRI contrast agents with extracellular matrix components N2 - In clinical magnetic resonance imaging, non-specific Gd-based contrast agents (GBCAs) are frequently used to improve image quality. However, the interaction of GBCAs with tissue components is not yet fully understood. Many diseases, including inflammation, fibrosis, and tumor invasion, are associated with characteristic changes of the extracellular matrix (ECM). The ECM is a three-dimensional scaffold that embeds the cells of all mammalian tissues. It is composed of structural proteins (e.g., collagen, elastin) and proteoglycans, which consist of glycosaminoglycans (GAGs) covalently bound to a protein core. GAGs are characterized by their ability to form complexes with cations such as lanthanides. Thus, GAGs could be a potential binding partner for GBCAs as a whole or for dechelated Gd. Laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA/ICP-ToF-MS) was used to investigate the uptake and distribution of different GBCAs in spheroids mimicking biological tissue and exhibiting different ECM expressions. Spheroids from Chinese hamster ovary (CHO) cells and GAG-depleted CRL-2242 cells were incubated with gadolinium chloride and various linear and macrocyclic GBCAs. Although all spheroids were exposed to identical Gd concentrations, differences in the amount of Gd taken up were observed. Gadolinium chloride is more strongly absorbed and accumulates mainly in the outer regions of the spheroids. In contrast, after incubation with linear and macrocyclic GBCAs, Gd is detected in the interior of both types of spheroids. Furthermore, differences in the Gd amount were found depending on the GBCA used. However, further studies are needed to elucidate such complex interactions, also using other (bio)analytical techniques. T2 - 2nd Annual Conference on Mass Spectrometry Imaging and Integrated Topics, IMSIS-2024 CY - Münster, Germany DA - 09.09.2024 KW - ICP-MS KW - Laser ablation KW - Contrast agent KW - Spheroid KW - Extracellular matrix PY - 2024 AN - OPUS4-61049 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Meermann, Björn A1 - Lisec, Jan A1 - Jaeger, Carsten A1 - Saatz, Jessica A1 - Traub, Heike A1 - von der Au, Marcus T1 - Mehr Analyten, kleinere Proben N2 - Molekülmassenspektrometrie entwickelt sich weg von klassischer Target- hin zu Nontarget-Analytik. Elementmassenspektrometrie liefert hohe Ortsauflösung beim Element-Imaging und analysiert einzelne Zellen. Aufgrund der Fortschritte bei den Geräten für Timeof-Flight-Massenspektrometrie mit induktiv gekoppeltem Plasma (ICPToF-MS) lässt sich das gesamte Periodensystem der Elemente in kurzen transienten Signalen quasi-simultan massenspektrometrisch erfassen. KW - Massenspektrometrie KW - Non-target KW - ICP-ToF-MS KW - Laser Ablation/Imaging PY - 2021 VL - 69 IS - Juni SP - 64 EP - 67 PB - Wiley-VCH AN - OPUS4-52800 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -