TY - JOUR A1 - Van Den Bossche, T. A1 - Kunath, B. A1 - Schallert, K. A1 - Schäpe, S. A1 - Abraham, P. E. A1 - Armengaud, J. A1 - Arntzen, M. Ø. A1 - Bassignani, A. A1 - Benndorf, D. A1 - Fuchs, S. A1 - Giannone, R. J. A1 - Griffin, T. J. A1 - Hagen, L. H. A1 - Halder, R. A1 - Henry, C. A1 - Hettich, R. L. A1 - Heyer, R. A1 - Jagtap, P. A1 - Jehmlich, N. A1 - Jensen, M. A1 - Juste, C. A1 - Kleiner, M. A1 - Langella, O. A1 - Lehmann, T. A1 - Leith, E. A1 - May, P. A1 - Mesuere, B. A1 - Miotello, G. A1 - Peters, S. L. A1 - Pible, O. A1 - Queiros, P. T. A1 - Reichl, U. A1 - Renard, B. Y. A1 - Schiebenhoefer, H. A1 - Sczyrba, A. A1 - Tanca, A. A1 - Trappe, K. A1 - Trezzi, J.-P. A1 - Uzzau, S. A1 - Verschaffelt, P. A1 - von Bergen, M. A1 - Wilmes, P. A1 - Wolf, M. A1 - Martens, L. A1 - Muth, Thilo T1 - Critical Assessment of MetaProteome Investigation (CAMPI): A multi-laboratory comparison of established workflows JF - Nature communications N2 - Metaproteomics has matured into a powerful tool to assess functional interactions in microbial communities. While many metaproteomic workflows are available, the impact of method choice on results remains unclear. Here, we carry out a community-driven, multi-laboratory comparison in metaproteomics: the critical assessment of metaproteome investigation study (CAMPI). Based on well-established workflows, we evaluate the effect of sample preparation, mass spectrometry, and bioinformatic analysis using two samples: a simplified, laboratory-assembled human intestinal model and a human fecal sample. We observe that variability at the peptide level is predominantly due to sample processing workflows, with a smaller contribution of bioinformatic pipelines. These peptide-level differences largely disappear at the protein group level. While differences are observed for predicted community composition, similar functional profiles are obtained across workflows. CAMPI demonstrates the robustness of present-day metaproteomics research, serves as a template for multi-laboratory studies in metaproteomics, and provides publicly available data sets for benchmarking future developments. KW - Metaproteomics KW - Mass spectrometry KW - Data science KW - Benchmarking KW - Bioinformatics PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-541220 DO - https://doi.org/10.1038/s41467-021-27542-8 SN - 2041-1723 VL - 12 SP - 1 EP - 15 PB - Nature Publishing Group CY - London AN - OPUS4-54122 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pott, U. A1 - Crasselt, Claudia A1 - Fobbe, N. A1 - Haist, M. A1 - Heinemann, M. A1 - Hellmann, S. A1 - Ivanov, D. A1 - Jakob, C. A1 - Jansen, D. A1 - Lei, L. A1 - Li, R. A1 - Link, J. A1 - Lowke, D. A1 - Mechtcherine, V. A1 - Neubauer, J. A1 - Nicia, D. A1 - Plank, J. A1 - Reißig, S. A1 - Schäfer, T. A1 - Schilde, C. A1 - Schmidt, Wolfram A1 - Schröfl, C. A1 - Sowoidnich, T. A1 - Strybny, B. A1 - Ukrainczyk, N. A1 - Wolf, J. A1 - Xiao, P. A1 - Stephan, D. T1 - Characterization data of reference materials used for phase II of the priority program DFG SPP 2005 “Opus Fluidum Futurum –Rheology of reactive, multiscale, multiphase construction materials” JF - Data in Brief N2 - A thorough characterization of base materials is the prereq- uisite for further research. In this paper, the characterization data of the reference materials (CEM I 42.5 R, limestone pow- der, calcined clay and a mixture of these three components) used in the second funding phase of the priority program 2005 of the German Research Foundation (DFG SPP 2005) are presented under the aspects of chemical and min- eralogical composition as well as physical and chemical properties. The data were collected based on tests performed by up to eleven research groups involved in this cooperative program. KW - Portland cement KW - Limestone powder KW - Calcined clay KW - Sustainable cement KW - DFG SPP 2005 PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-569913 DO - https://doi.org/10.1016/j.dib.2023.108902 VL - 47 SP - 1 EP - 19 PB - Elsevier AN - OPUS4-56991 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Wolf, S. A1 - McMahon, Dino Peter A1 - Lim, K.S. A1 - Pull, C.D. A1 - Clark, S.J. A1 - Paxton, R.J. A1 - Osborne, J.L. T1 - So near and yet so far: harmonic radar reveals reduced homing ability of Nosema infected honeybees JF - PLoS one N2 - Pathogens may gain a fitness advantage through manipulation of the behaviour of their hosts. Likewise, host behavioural changes can be a defence mechanism, counteracting the impact of pathogens on host fitness. We apply harmonic radar technology to characterize the impact of an emerging pathogen - Nosema ceranae (Microsporidia) - on honeybee (Apis mellifera) flight and orientation performance in the field. Honeybees are the most important commercial pollinators. Emerging diseases have been proposed to play a prominent role in colony decline, partly through sub-lethal behavioural manipulation of their hosts. We found that homing success was significantly reduced in diseased (65.8%) versus healthy foragers (92.5%). Although lost bees had significantly reduced continuous flight times and prolonged resting times, other flight characteristics and navigational abilities showed no significant difference between infected and non-infected bees. Our results suggest that infected bees express normal flight characteristics but are constrained in their homing ability, potentially compromising the colony by reducing its resource inputs, but also counteracting the intra-colony spread of infection. We provide the first high-resolution analysis of sub-lethal effects of an emerging disease on insect flight behaviour. The potential causes and the implications for both host and parasite are discussed. PY - 2014 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-328827 DO - https://doi.org/10.1371/journal.pone.0103989 SN - 1932-6203 VL - 9 IS - 8 SP - e103989, 1 EP - 15 PB - PubMed Central AN - OPUS4-32882 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tjaden, B. A1 - Baum, K. A1 - Marquardt, V. A1 - Simon, M. A1 - Trajkovic-Arsic, M. A1 - Kouril, T. A1 - Siebers, B. A1 - Lisec, Jan A1 - Siveke, J. T. A1 - Schulte, J. H. A1 - Benary, U. A1 - Remke, M. A1 - Wolf, J. A1 - Schramm, A. T1 - MYCN-induced metabolic rewiring creates novel therapeutic vulnerabilities in neuroblastoma JF - bioRxiv : The preprint server for biology N2 - MYCN is a transcription factor that is aberrantly expressed in many tumor types and is often correlated with poor patient prognosis. Recently, several lines of evidence pointed to the fact that oncogenic activation of MYC family proteins is concomitant with reprogramming of tumor cells to cope with an enhanced need for metabolites during cell growth. These adaptions are driven by the ability of MYC proteins to act as transcriptional amplifiers in a tissue-of-origin specific manner. Here, we describe the effects of MYCN overexpression on metabolic reprogramming in neuroblastoma cells. Ectopic expression of MYCN induced a glycolytic switch that was concomitant with enhanced sensitivity towards 2-deoxyglucose, an inhibitor of glycolysis. Moreover, global metabolic profiling revealed extensive alterations in the cellular metabolome resulting from overexpression of MYCN. Limited supply with either of the two main carbon sources, glucose or glutamine, resulted in distinct shifts in steady-state metabolite levels and significant changes in glutathione metabolism. Interestingly, interference with glutamine-glutamate conversion preferentially blocked proliferation of MYCN overexpressing cells, when glutamine levels were reduced. Thus, our study uncovered MYCN induction and nutrient levels as important metabolic master switches in neuroblastoma cells and identified critical nodes that restrict tumor cell proliferation. KW - Mass-spectrometry KW - Tumor metabolism KW - MYCN PY - 2018 DO - https://doi.org/10.1101/423756 SP - 1 EP - 21 PB - Cold Spring Harbor Laboratory CY - Cold Spring Harbor, NY AN - OPUS4-46815 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sturgeon, R. E. A1 - Wahlen, R. A1 - Brandsch, T. A1 - Fairman, B. A1 - Wolf-Briche, C. A1 - Alonso, J. I. G. A1 - González, P. R. A1 - Encinar, J. R. A1 - Sanz-Medel, A. A1 - Inagaki, K. A1 - Takatsu, A. A1 - Lalere, B. A1 - Monperrus, M. A1 - Zuloaga, O. A1 - Krupp, E. A1 - Amouroux, D. A1 - Donard, O. F. X. A1 - Schimmel, H. A1 - Sejeroe-Olsen, B. A1 - Konieczka, P. A1 - Schultze, P. A1 - Taylor, P. A1 - Hearn, R. A1 - Mackay, L. A1 - Myors, R. A1 - Win, Tin A1 - Liebich, Almuth A1 - Philipp, Rosemarie A1 - Yang, L. A1 - Willie, S. T1 - Determination of tributyltin in marine sediment: Comité Consultatif pour la Quantité de Matière (CCQM) pilot study P-18 international intercomparison JF - Analytical and bioanalytical chemistry N2 - The capabilities of National Metrology Institutes (NMIs—those which are members of the Comité Consultatif pour la Quantité de Matière (CCQM)of the CIPM) and selected outside "expert" laboratories to quantitate (C4H9)3Sn+ (TBT) in a prepared marine sediment were assessed. This exercise was sanctioned by the 7th CCQM meeting, April 4–6, 2001, as an activity of the Inorganic Analysis Working Group and was jointly piloted by the Institute for National Measurement Standards of the National Research Council of Canada (NRC) and the Laboratory of the Government Chemist (LGC), UK. A total of 11 laboratories submitted results (7 NMIs, and 4 external labs). Two external laboratories utilized a standard calibration approach based on a natural abundance TBT standard, whereas all NMIs relied upon isotope dilution mass spectrometry for quantitation. For this purpose, a species specific 117Sn-enriched TBT standard was supplied by the LGC. No sample preparation methodology was prescribed by the piloting laboratories and, by consequence, a variety of approaches was adopted by the participants, including mechanical shaking, sonication, accelerated solvent extraction, microwave assisted extraction and heating in combination with Grignard derivatization, ethylation and direct sampling. Detection techniques included ICP–MS (with GC and HPLC sample introduction), GC–MS, GC–AED and GC–FPD. Recovery of TBT from a control standard (NRCC CRM PACS-2 marine sediment) averaged 93.5±2.4% (n=14). Results for the pilot material averaged 0.680±0.015 µmol kg–1 (n=14; 80.7±1.8 µg kg–1) with a median value of 0.676 µmol kg–1. Overall, performance was substantially better than state-of-the-art expectations and the satisfactory agreement amongst participants permitted scheduling of a follow-up Key comparison for TBT (K-28), a Pilot intercomparison for DBT (P-43), and certification of the test sediment for TBT content and its release as a new Certified Reference Material (HIPA-1) with a TBT content of 0.679±0.089 µmol kg–1 (expanded uncertainty, k=2, as Sn) (80.5±10.6 µg kg–1). Electronic Supplementary Material Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s00216-003-2016-9. KW - Tributyltin KW - Marine sediment KW - International intercomparison PY - 2003 DO - https://doi.org/10.1007/s00216-003-2016-9 SN - 1618-2642 SN - 1618-2650 VL - 376 IS - 6 SP - 780 EP - 787 PB - Springer CY - Berlin AN - OPUS4-10905 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Rütters, H. A1 - Fischer, S. A1 - Le, Quynh Hoa A1 - Bettge, Dirk A1 - Bäßler, Ralph A1 - Maßmann, J. A1 - Ostertag-Henning, C. A1 - Wolf, J. L. A1 - Pumpa, M. A1 - Lubenau, U. A1 - Knauer, S. A1 - Jaeger, P. A1 - Neumann, A. A1 - Svensson, K. A1 - Pöllmann, H. A1 - Lempp, C. A1 - Menezes, F. F. A1 - Hagemann, B. T1 - Towards defining reasonable minimum composition thresholds – Impacts of variable CO2 stream compositions on transport, injection and storage JF - International journal of greenhouse gas control N2 - To set up recommendations on how to define “reasonable minimum composition thresholds” for CO2 streams to access CO2 pipeline networks, we investigated potential impacts of CO2 streams with different and temporally variable compositions and mass flow rates along the CCS chain. All investigations were based on a generic “CCS cluster scenario” in which CO2 streams captured from a spatial cluster of eleven emitters (seven fossil-fired power plants, two cement plants, one refinery and one steel mill) are collected in a regional pipeline network. The resulting CO2 stream (19.78 Mio t impure CO2 per year) is transported in a trunk line (onshore and offshore) and injected into five generic replicate storage structures (Buntsandstein saline aquifers) offshore. Experimental investigations and modeling of selected impacts revealed beneficial as well as adverse impacts of different impurities and their combinations. Overall, no fundamental technical obstacles for transporting, injecting and storing CO2 streams of the considered variable compositions and mass flow rates were observed. We recommend to define minimum composition thresholds for each specific CCS project through limiting i) the overall CO2 content, ii) maximum contents of relevant impurities or elements, iii) acceptable variability of concentrations of critical impurities, and defining impurity combinations to be avoided. KW - Impurities KW - CO2 quality KW - Pipeline network KW - Whole-chain CCS scenario KW - Recommendations PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-543004 DO - https://doi.org/10.1016/j.ijggc.2022.103589 SN - 1750-5836 VL - 114 SP - 1 EP - 14 PB - Elsevier CY - New York, NY AN - OPUS4-54300 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Algernon, D. A1 - Arndt, R. A1 - Ebsen, B. A1 - Feistkorn, S. A1 - Friese, M. A1 - Große, C. A1 - Kathage, S. A1 - Keßler, S. A1 - Kurz, J. A1 - Küttenbaum, Stefan A1 - Lohse, C. A1 - Maack, Stefan A1 - Niederleithinger, Ernst A1 - Schickert, M. A1 - Schröder, G. A1 - Taffe, A. A1 - Walther, A. A1 - Wilcke, M. A1 - Wolf, J. A1 - Wöstmann, Jens T1 - Richtlinie B-LF 01: Leitfaden zur Erstellung von Prüfanweisungen für die Zerstörungsfreie Prüfung im Bauwesen (ZfP Bau) N2 - Der vorliegende Leitfaden dient zur Unterstützung der Entwicklung und Umsetzung von Prüfanweisungen für ZfP-Verfahren im Bauwesen. Er gibt einen Überblick über Verwendungszweck, Erstellung und Inhalte von Prüfanweisungen unter Berücksichtigung einheitlicher Standardisierungsziele. KW - Prüfanweisung KW - Beton KW - Leitfaden PY - 2022 SN - 978-3-947971-23-7 SP - 1 EP - 9 PB - Deutsche Gesellschaft für Zerstörungsfreie Prüfung e.V. (DGZfP) CY - Berlin ET - 1. Aufl., April 2022 AN - OPUS4-54985 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rütters, H. A1 - Fischer, S. A1 - Le, Quynh A1 - Bettge, Dirk A1 - Bäßler, Ralph A1 - Maßmann, J. A1 - Ostertag-Henning, C. A1 - Wolf, J. Lennard A1 - Pumpa, M. A1 - Lubenau, U. A1 - Knauer, S. A1 - Jaeger, P. A1 - Neumann, A. A1 - Svensson, K. A1 - Lempp, C. A1 - Menezes, F. A1 - Hagemann, B. T1 - Towards defining reasonable minimum composition thresholds – impacts of variable CO2 stream compositions on transport, injection and storage T2 - 15th International Conference on Greenhouse Gas Control Technologies, GHGT-15 N2 - The collaborative project “Impacts of impurities in CO2 streams captured from different emitters in a regional cluster on transport, injection and storage (CLUSTER)” aimed to set up recommendations on how to define “reasonable minimum composition thresholds” that CO2 streams should meet when accessing CO2 transport pipeline networks. Within CLUSTER, we investigated potential impacts of CO2 streams with different and temporally variable compositions and mass flow rates along the whole CCS chain. Investigations included, amongst others, impacts on: Corrosion of pipeline steel, pipeline network design and related transport costs, alteration of well bore cements, pressure development and rock integrity, geochemical reactions, and petrophysical and geomechanical rock properties. All investigations are based on a generic CCS chain scenario. In this scenario, CO2 streams are captured from a spatial cluster of eleven emitters and collected in a regional pipeline network. Emitters comprise seven fossil fuel-fired power plants equipped with different capture technologies, two cement plants, one refinery and one integrated iron and steel plant. In total, 19.78 Mio t CO2 (including impurities) are captured in the emitter cluster annually. The combined CO2 stream is transported in a trunk line with a length of 400 km (100 km of these offshore) and is injected into five generic storage structures. The storage reservoirs are saline aquifers of the Buntsandstein. The investigations revealed beneficial and deteriorating impacts of different impurities and combinations thereof. Overall, no fundamental technical obstacles for transporting, injecting and storing CO2 streams of the modelled variable compositions and mass flow rates were observed. Based on the results, the CLUSTER project team recommends not to define “minimum composition thresholds” for CO2 streams as strict threshold values for each individual impurity in the stream. Instead, CO2 stream compositions and variabilities for specific CCS projects should be constrained with regard to a set of parameters including i) the overall CO2 content, ii) maximum contents of relevant impurities or elements, iii) acceptable variability of CO2 stream composition, and iv) impurity combinations to be avoided. T2 - 15th International Conference on Greenhouse Gas Control Technologies, GHGT-15 CY - Online meeting DA - 15.03.2021 KW - Corrosion KW - Impurities KW - CO2 quality KW - Pipeline network KW - Whole-chain CCS scenario KW - Recommendations PY - 2021 DO - https://doi.org/10.2139/ssrn.3816427 SP - 1 EP - 18 AN - OPUS4-52418 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rütters, H A1 - Fischer, S A1 - Le, Quynh Hoa A1 - Bettge, Dirk A1 - Bäßler, Ralph A1 - Maßmann, J A1 - Ostertag-Henning, C A1 - Lennard Wolf, J A1 - Pumpa, M A1 - Lubenau, U A1 - Knauer, S A1 - Jaeger, P A1 - Neumann, A A1 - Svensson, K A1 - Pöllmann, H A1 - Lempp, C A1 - Menezes, F A1 - Hagemann, B T1 - Towards Defining Reasonable Minimum Composition Thresholds – Impacts of Variable CO2 Stream Compositions on Transport, Injection and Storage T2 - Proceedings of the 15th Greenhouse Gas Control Technologies Conference 15-18 March 2021 N2 - The collaborative project “Impacts of impurities in CO2 streams captured from different emitters in a regional cluster on transport, injection and storage (CLUSTER)” aimed to set up recommendations on how to define “reasonable minimum composition thresholds” that CO2 streams should meet when accessing CO2 transport pipeline networks. Within CLUSTER, we investigated potential impacts of CO2 streams with different and temporally variable compositions and mass flow rates along the whole CCS chain. Investigations included, amongst others, impacts on: • corrosion of pipeline steel, • pipeline network design and related transport costs, • alteration of well bore cements, • pressure development and rock integrity, • geochemical reactions, and • petrophysical and geomechanical rock properties. All investigations are based on a generic CCS chain scenario. In this scenario, CO2 streams are captured from a spatial cluster of eleven emitters and collected in a regional pipeline network. Emitters comprise seven fossil fuel-fired power plants equipped with different capture technologies, two cement plants, one refinery and one integrated iron and steel plant. In total, 19.78 Mio t CO2 (including impurities) are captured in the emitter cluster annually. The combined CO2 stream is transported in a trunk line with a length of 400 km (100 km of these offshore) and is injected into five generic storage structures. The storage reservoirs are saline aquifers of the Buntsandstein. The investigations revealed beneficial and deteriorating impacts of different impurities and combinations thereof. Overall, no fundamental technical obstacles for transporting, injecting and storing CO2 streams of the modelled variable compositions and mass flow rates were observed. Based on the results, the CLUSTER project team recommends not to define “minimum composition thresholds” for CO2 streams as strict threshold values for each individual impurity in the stream. Instead, CO2 stream compositions and variabilities for specific CCS projects should be constrained with regard to a set of parameters including i) the overall CO2 content, ii) maximum contents of relevant impurities or elements, iii) acceptable variability of CO2 stream composition, and iv) impurity combinations to be avoided. T2 - The 15th Greenhouse Gas Control Technologies Conference CY - Online meeting DA - 15.03.2021 KW - Corrosion KW - Impurities KW - CO2 quality KW - Pipeline network KW - Whole-chain CCS scenario KW - Recommendations PY - 2021 DO - https://doi.org/10.2139/ssrn.3816427 SP - 1 EP - 18 PB - Elservier AN - OPUS4-52940 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Berger, Achim A1 - Görner, Wolf A1 - Merchel, Silke A1 - Radtke, Martin A1 - Riesemeier, Heinrich A1 - Reiche, I. A1 - Riederer, J. T1 - A closer look at Albrecht Dürer's drawings (1471-1528) T2 - BESSY highlights 2001 PY - 2002 UR - http://www.helmholtz-berlin.de/media/media/oea/web/pr_webseite/druckschriften/infos/bessy/highlights2001.pdf#page=32 SN - 0179-4159 SP - 32 EP - 33 PB - Berliner Elektronenspeicherring-Gesellschaft für Synchrotronstrahlung CY - Berlin AN - OPUS4-1323 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -