TY - JOUR A1 - Gay-Fraret, J. A1 - Ardissone, S. A1 - Kambara, K. A1 - Broughton, William J. A1 - Deakin, W.J. A1 - Le Quéré, A. T1 - Cyclic-Beta-glucans of Rhizobium (Sinorhizobium) sp. strain NGR234 are required for hypo-osmotic adaption, motility, and efficient symbiosis with host plants JF - FEMS microbiology letters N2 - Cyclic-β-glucans (CβG) consist of cyclic homo-polymers of glucose that are present in the periplasmic space of many Gram-negative bacteria. A number of studies have demonstrated their importance for bacterial infection of plant and animal cells. In this study, a mutant of Rhizobium (Sinorhizobium) sp. strain NGR234 (NGR234) was generated in the cyclic glucan synthase (ndvB)-encoding gene. The great majority of CβG produced by wild-type NGR234 are negatively charged and substituted. The ndvB mutation abolished CβG biosynthesis. We found that, in NGR234, a functional ndvB gene is essential for hypo-osmotic adaptation and swimming, attachment to the roots, and efficient infection of Vigna unguiculata and Leucaena leucocephala. KW - Cyclic Beta-(1,2)-glucans KW - Legumes KW - Osmotic adaption KW - Swimming KW - Nodulation KW - ndvB PY - 2012 DO - https://doi.org/10.1111/j.1574-6968.2012.02595.x SN - 0378-1097 SN - 1574-6968 VL - 333 IS - 1 SP - 28 EP - 36 PB - Wiley-Blackwell CY - Malden, Mass., USA AN - OPUS4-26111 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -