TY - JOUR A1 - Tvrdonova, M. A1 - Vlcnovska, M. A1 - Pompeiano Vanickova, L. A1 - Kanicky, V. A1 - Adam, V. A1 - Ascher, Lena A1 - Jakubowski, Norbert A1 - Vaculovicova, M. A1 - Vaculovic, T. T1 - Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry N2 - In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling. KW - Nanoparticle KW - LA-ICP-MS KW - Labeling PY - 2019 DO - https://doi.org/10.1007/s00216-018-1300-7 SN - 1618-2642 SN - 1618-2650 VL - 411 IS - 3 SP - 559 EP - 564 PB - Springer-Verlag GmbH CY - Berlin, Heidelberg AN - OPUS4-47093 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Tvrdoňová, M. A1 - Ascher, Lena A1 - Jakubowski, Norbert A1 - Vaculovičová, M. A1 - Moravanská, A. A1 - Vaněčková, T. A1 - Vaculovič, T. T1 - A new strategy of reagents labeling (NPs) used in immunoassay with LA-ICP-MS N2 - Laser ablation with inductively coupled plasma is still more used in life science as biology and biomedicine and the utilization of metals and proteins determination simultaneously is also growing up. We have developed a new strategy of labeling of antibody (it can specific binds to proteins) by nanoparticles and quantum dots which is composed of thousands of atoms and thus increases the sensitivity enormously and of course decreases the Limit of detection, compare to lanthanoids labeling. The ability of successfully tagged antibodies bound to Antigen (protein) was proved by dot blot on membrane imaged by LA-ICP-MS. T2 - Euroanalysis 2017 CY - Stockholm, Sweden DA - 28.08.2017 KW - Immunoassay KW - LA-ICP-MS KW - Labeling KW - Nanoparticle PY - 2017 AN - OPUS4-43168 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -