TY - CONF A1 - Fischer, Tobias A1 - Hecht, M. A1 - Dietrich, P. A1 - Unger, Wolfgang A1 - Nutsch, A. A1 - Streeck, C. A1 - Weser, J. A1 - Beckhoff, B. A1 - Rurack, Knut T1 - Application of dual-mode XPS and fluorescence labels in the quantification of surface functional groups T2 - 15th European Conference on Applications of Surface and Interface Analysis 2013 (ECASIA'13) CY - Sardinia, Italy DA - 2013-10-13 PY - 2013 AN - OPUS4-29566 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gawlitza, Kornelia A1 - Fischer, Tobias A1 - Rurack, Knut T1 - Chemical functionalization for quantitative spectroscopic labeling on macroscopically flat surfaces N2 - This chapter highlights the application of chemical derivatization (CD) to facilitate the quantification of surface functional groups being an important issue for a wide field of applications. The selective attachment of a chemical label to a surface functional group being afterwards exclusively detectable by a highly sensitive technique overcomes the problem of characterizing low amounts of functional groups on macroscopically flat surfaces. The most frequently employed methods include CD X-ray photoelectron spectroscopy, ultraviolet/visible absorption, and fluorescence spectroscopy, as well as time-of-flight secondary ion mass spectrometry. Herein, the basic conditions for the different techniques regarding the specific surface functional group which need to be quantified are discussed. Additionally, the substrate highly influences the compatibility of the corresponding method. Because not just the quantification but also the preparation of the desired application is important, a summary of different preparation methods for glass, polymer and gold substrates is presented. KW - Chemical derivatization KW - Fluorescence KW - Surface group quantification KW - Time-of-flight secondary ion mass spectrometry KW - UV/vis spectroscopy KW - X-ray photoelectron spectroscopy PY - 2018 SN - 978-0-12-409547-2 U6 - https://doi.org/10.1016/B978-0-12-409547-2.13191-9 SP - 1 PB - Elsevier AN - OPUS4-43767 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Fischer, Tobias A1 - Hecht, Mandy A1 - Dietrich, Paul A1 - Unger, Wolfgang A1 - Rurack, Knut T1 - Development of a dual XPS and Fluorescence Label for the Analysis of Surface Amino Groups T2 - ThGOT, 8. Thementage Grenz und Oberflächentechnik CY - Leipzig, Germany DA - 2012-09-04 PY - 2012 AN - OPUS4-26850 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Descalzo López, Ana Belén A1 - Zhu, Shengchao A1 - Fischer, Tobias A1 - Rurack, Knut ED - Demchenko, A.P. T1 - Optimization of the coupling of target recognition and signal generation N2 - Fluorescent reporters that literally "light up" upon binding of a target species are particularly interesting from an analytical point of view. This contribution introduces the main signaling concepts in fluorescent probe research, discusses strategies toward their optimization in terms of signal output, and highlights the wealth of alternative protocols that has been realized in the past two decades to create signaling systems with luminescence amplification features. KW - Dyes KW - Fluorescence KW - FRET KW - Molecular Recognition KW - Nanoparticles PY - 2010 SN - 978-3-642-04699-5 U6 - https://doi.org/10.1007/978-3-642-04701-5_2 N1 - Serientitel: Springer Series on Fluorescence – Series title: Springer Series on Fluorescence VL - 9 IS - 1 SP - 41 EP - 106 PB - Springer AN - OPUS4-21941 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Fischer, Tobias A1 - Hecht, Mandy A1 - Dietrich, Paul A1 - Unger, Wolfgang A1 - Rurack, Knut T1 - Quantifizierung von Oberflächenaminogruppen mithilfe eines dualen XSP- und Fluoreszenzlabel T2 - 8. Deutsches BioSensor Symposium CY - Wildau, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-27848 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Fischer, Tobias A1 - Hecht, Mandy A1 - Dietrich, Paul A1 - Unger, Wolfgang A1 - Rurack, Knut T1 - Towards Quantitaive Surface Amino Group Analysis with One Combined Flourescence and XPS Label T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-27852 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -