TY - CONF A1 - Behnke, Thomas A1 - Mathejczyk, J. A1 - Würth, Christian A1 - Napp, J. A1 - Hoffmann, Katrin A1 - Alves, F. A1 - Schäferling, M. A1 - Resch-Genger, Ute A1 - Lehmann, Andreas T1 - Design and Bioanalytical Applications of NIR-Fluorescent Polystyrene Nanoparticles T2 - Imaging Innovations of the Lung CY - Göttingen, Germany DA - 2012-04-18 PY - 2012 AN - OPUS4-25698 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Behnke, Thomas A1 - Quevedo, Pablo A1 - Moser, Marko A1 - Würth, Christian A1 - Resch-Genger, Ute T1 - Design and Characterization of Streptavidin Modified Fluorescent Nanoparticles T2 - Molekulare Bildgebung 2014 CY - Jena, Germany DA - 2014-10-08 PY - 2014 AN - OPUS4-31613 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Behnke, Thomas A1 - Hoffmann, Katrin A1 - Moser, Marko A1 - Markus, A. A1 - Drescher, D. A1 - Kneipp, J. A1 - Alves, F. A1 - Resch-Genger, Ute T1 - FLIM and Optical Imaging with NIR-Fluorescent Lifetime-Encoded Nanoparticles of well Known Surface Chemistry T2 - Anakon 2015 CY - Graz, Austria DA - 2015-03-23 PY - 2015 AN - OPUS4-33695 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Behnke, Thomas A1 - Moser, Marko A1 - Quevedo, Pablo A1 - Resch-Genger, Ute T1 - Fluoreszente Polymer-Nanopartikel für die Bioanalytik T2 - Nano- und Mikropartikel für die biomedizinische Analytik CY - Potsdam-Golm DA - 2015-10-29 PY - 2015 AN - OPUS4-34723 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Behnke, Thomas A1 - Moser, Marko A1 - Nirmalananthan-Budau, Nithiya A1 - Resch-Genger, Ute T1 - Spectroscopic quantification of surface groups on micro- and nanoparticles N2 - Nanometer- and micrometer-sized particles are of increasing importance for a wide range of applications in the material and life sciences. This includes carriers for, e.g., drugs as well as dye molecules for use as multichromophoric reporters for signal enhancement in optical assays, platforms for DNA sequencing, and the fabrication of nanosensors and targeted probes for bioimaging studies. Application-relevant properties of such particles include their size (and size distribution), shape, colloidal stability, biocompatibility, and ease of subsequent functionalization, e.g., with linkers, sensor molecules, and targeting ligands. The latter requires knowledge of the number of groups effectively accessible for subsequent coupling reactions and hence, selective and sensitive methods of analysis, which can be ideally employed for the characterization of a broad variety of particle systems independent of their optical properties, i.e., scattering or the presence of encoding dyes. For product control, robust, reliable and fast methods performable with inexpensive equipment are prefered. In this respect, we studied a variety of conventional labels for optical readout, utilizing changes in intensity and/or color of absorption and/or emission. In addition, we developed a platform of cleavable and multimodal labels for optical assays which consist of a cleavable linker and an optically active reporter moiety. In contrast to conventional reporters measured directly at the particle surface, which are prone to signal distortions by scattering and encoding dyes, these cleavable labels can be detected colorimetrically or fluorometrically both bound at the particle surface and after quantitative cleavage of the linker in the transparent supernatant. Moreover, they enable straightforward validation by method comparison with elemental analysis, ICP-OES or ICP-MS. Here, we present representative examples of newly synthesized cleavable labels and their application for the quantification of amino, thiol and carboxy surface groups on different nanomaterials and compare these results with measurements using conventional optical labels. T2 - 253rd ACS National Meeting CY - San Francisco, CA, USA DA - 02.04.2017 KW - Nanoparticles KW - Microparticles KW - Surface groups KW - Quantification KW - Cleavable linker PY - 2017 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-40004 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -