TY - JOUR A1 - Ismail, N. S. A1 - Le, Quynh Hoa A1 - Vu, T. H. A1 - Inoue, Yuki A1 - Yoshikawa, H. A1 - Saito, M. A1 - Tamiya, E. T1 - Electrochemiluminescence Based Enzymatic Urea SensorUsing Nanohybrid of Isoluminol-gold Nanoparticle-graphene Oxide Nanoribbons JF - Electroanalysis N2 - This study evaluates on the possibility of using gold nanoparticles functionalizedwith the luminol derivative N-(aminobutyl)-N-(ethylisoluminol)(ABEI) and hybridizedwith graphene oxidenanoribbons on acarbon based screen-printedelectrode (ABEI-AuNP-GONR/SPE) as an enzymaticelectrochemiluminescence (ECL) urea sensor.The electrocatalytic activity and ECL intensity of ABEI-AuNP-GONR/SPE were found to increase proportionally with the concentrationofurea in the analyte sample,owing to the rise in pH value.These phenomena are attributed to increased formation of luminol monoanion precursors for further electrochemical oxidation,which in turn produce eitherluminol radicals or excited3-amino-phthalate molecules.The luminescence is most likely caused by the interaction of luminol radicals with superoxide radicals formed from dissolved oxygen. Thesensitivity of our sensor was determinedtobe 170.58 mM@1 and 16.23 mM@1 for urea concentrations from 2to5.82 mM and from 5.82 to 30 mM, respectively, coveringthe normal urea level in human blood. KW - Nanomaterials KW - Graphene oxide KW - Biosensors PY - 2017 UR - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600477 DO - https://doi.org/10.1002/elan.201600477 SN - 1040-0397 SN - 1521-4109 VL - 29 IS - 4 SP - 938 EP - 943 PB - Wiley-VCH Verlag GmbH & Co. KGaA CY - Weinheim AN - OPUS4-40970 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -