TY - CONF A1 - Gärtner, Stefanie A1 - Carvalho, José Joao A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Development of an immunoassay for the anthropogenic marker coprostanol T2 - 6. Interdiziplinäres Doktorandenseminarder GDCh CY - Berlin, Germany DA - 2012-02-26 PY - 2012 AN - OPUS4-25545 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gärtner, Stefanie A1 - Höhne, Robert A1 - Schneider, Rudolf A1 - Garbe, L.-A. T1 - Antibodies for the anthropogenic marker isolithocholic acid and their application in ELISA and microarrays T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-27815 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gärtner, Stefanie A1 - Höhne, Robert A1 - Carvalho, José Joao A1 - Schneider, Rudolf A1 - Garbe, L.-A. T1 - Immunoassays for the anthropogenic marker isolithocholic acid T2 - Pharmaceutical Products in the Environment - Is there a problem?" CY - Nimes, France DA - 2013-06-03 PY - 2013 AN - OPUS4-28942 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gärtner, Stefanie A1 - Canitz, Carsten A1 - Leif-Alexander, A1 - Schneider, Rudolf T1 - Challenges in the antibody production for the phthalate substitute DINCH T2 - XVII euroANALYSIS CY - Warsaw, Poland DA - 2013-08-25 PY - 2013 AN - OPUS4-28996 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gärtner, Stefanie A1 - Schneider, Rudolf A1 - Canitz, Carsten A1 - Leif-Alexander, T1 - Hapten design for the production of antibodies small molecules T2 - Trends in Diagnostics CY - Tübingen, Germany DA - 2013-10-07 PY - 2013 AN - OPUS4-29237 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gärtner, Stefanie A1 - Schneider, Rudolf A1 - Carvalho, J. J. A1 - Garbe, L.-A. T1 - A direct competitive ELISA for the anthropogenic marker isolithocholic acid T2 - 7th European Conference on Pesticides and Related Organic Micropollutants in the Environment CY - Oporto, Portugal DA - 2012-10-07 PY - 2012 AN - OPUS4-26671 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gärtner, Stefanie A1 - Carvalho, J.J. A1 - Emmerling, Franziska A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Polyclonal murine and rabbit antibodies for the bile acid isolithocholic acid N2 - Bile acids are relevant markers for clinical research. This study reports the production of antibodies for isolithocholic acid, the isomer of the extensively studied lithocholic acid. The IgG titer and affinity maturation were monitored during the immunizations of three mice and two rabbits. In both animal models, polyclonal antibodies with a high selectivity and affinity were produced. The development of a direct competitive ELISA with a test midpoint of 0.69 ± 0.05 µg/L and a measurement range from 0.09–15 µg/L is reported. Additionally, the crystal structure of isolithocholic acid is described for the first time. KW - ELISA KW - Crystal structure KW - Competitive assay KW - Immunization monitoring KW - 3ß-hydroxy-5ß-cholanic acid PY - 2015 U6 - https://doi.org/10.1080/15321819.2014.924419 SN - 1532-1819 SN - 1532-4230 VL - 36 IS - 3 SP - 233 EP - 252 PB - Taylor & Francis CY - Philadelphia, Pa. AN - OPUS4-31836 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gärtner, Stefanie T1 - Analytical methods for the phthalate substitutes DINCH and DEHT T2 - 2. BAM/BfR-Kolloquium CY - Berlin DA - 2014-10-16 PY - 2014 AN - OPUS4-31803 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -