TY - CONF A1 - Hösl, Simone A1 - Jakubowski, Norbert A1 - Scheler, Ch. A1 - Sauter, G. A1 - Linscheid, M. A1 - Scheler, Ch. A1 - Müller, Larissa A1 - Neumann, B. T1 - Development and application of metal-tagged antibodies for efficient diagnostic methods T2 - 11th European Workshop on Lasern Ablation CY - Gijón, Spain DA - 2012-06-18 PY - 2012 N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. AN - OPUS4-27351 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Jakubowski, Norbert A1 - Hösl, Simone A1 - Scheler, C. A1 - Roos, P.H. A1 - Linscheid, M.W. T1 - Comparison of different chelates for lanthanide labeling of antibodies and application in a Western blot immunoassay combined with detection by laser ablation (LA-)ICP-MS N2 - We have developed lanthanide labeling strategies for antibodies to adapt conventional biochemical workflows like Western blot immunoassays for detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) analysis with a special interest to apply the multi-element capabilities of ICP-MS for the design of multiplexed immunoassays. In this paper the lanthanide labeling of antibodies with MeCAT was investigated and the reaction conditions were optimized for application in a Western blot immunoassay analyzed by LA-ICP-MS. Furthermore, the MeCAT labeling strategy was compared with two other commercially available labeling reagents, MAXPAR™ and SCN-DOTA. As a proof-of-principle experiment chemically induced alterations of cytochrome P450 protein expression were investigated and the suitability of the differentially labeled antibodies for Western blot immunoassays of a complex liver microsomal protein fraction was tested. Limits of detection (LODs) in the lower fmol range were reached in the Western blot application using MeCAT and MAXPAR™ as element labeling reagents, whereas even sub-fmol LODs can be achieved in a dot blot experiment for the pure antibodies. PY - 2012 DO - https://doi.org/10.1039/c2ja30068k SN - 0267-9477 SN - 1364-5544 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. VL - 27 IS - 8 SP - 1311 EP - 1320 PB - Royal Society of Chemistry CY - London AN - OPUS4-26256 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hösl, Simone A1 - Müller, Larissa A1 - Scheler, Ch. A1 - Sauter, G. A1 - Schlüter, H A1 - Jakubowski, Norbert A1 - Linscheid, M. T1 - Development and application of metal-tagged antibodies for efficient diagnostic methods T2 - 11th European Workshop on Laser Ablation CY - Gijón, Spain DA - 2012-06-16 PY - 2012 N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. AN - OPUS4-26473 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hösl, Simone A1 - Neumann, B. A1 - Techritz, Sandra A1 - Linscheid, M. A1 - Theuring, F. A1 - Scheler, C. A1 - Jakubowski, Norbert A1 - Müller, Larissa T1 - Development of a calibration and standardization procedure for LA-ICP-MS using a conventional ink-jet printer for quantification of proteins in electro- and western-blot assays N2 - We developed new procedures for internal standardization and calibration to be used for laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for elemental micro mapping imaging of biological samples like Western blot membranes and tissue sections. These procedures are based on printing of metal spiked inks onto the top of thin layer samples for simultaneous internal standardization and calibration of LA-ICP-MS. In the case of internal standardization the ink is spiked with indium as an internal standard and homogenously printed over the entire membrane (size 56 cm2) prior to LA-ICP-MS detection, a standard deviation (RSD) value of 2% was achieved. In the second approach the metal content of lanthanide tagged proteins and antibodies after biological work flows was quantified by LA-ICP-MS on nitro-cellulose membranes. In this case the inks spiked with varying metals were printed with different densities on the same nitrocellulose membranes in well-defined squares to produce matrix-matched calibration standards. For validation and calibration the ink squares were excised and the specific metal content was measured by liquid ICP-MS after solubilization of the membrane slice. For the printed calibration standard limits of detection (LOD) of <4 fmol for different metals and relative process standard deviations of 1–2% only were determined via LA-ICP-MS. PY - 2014 DO - https://doi.org/10.1039/c4ja00060a SN - 0267-9477 SN - 1364-5544 VL - 29 IS - 7 SP - 1282 EP - 1291 PB - Royal Society of Chemistry CY - London AN - OPUS4-31171 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hösl, Simone A1 - Scheler, Ch. A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Linscheid, M. T1 - Quantitative detection of antibodies via metal coded tagging of the hinge region T2 - Tag der Chemie, Humboldt-Universität zu Berlin CY - Berlin, Germany DA - 2011-07-13 PY - 2011 N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. AN - OPUS4-24107 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hösl, Simone A1 - Neumann, B. A1 - Müller, Larissa A1 - Scheler, Ch. A1 - Theuring, F. A1 - Sauter, G. A1 - Schlüter, H A1 - Weller, Michael G. A1 - Linscheid, M. A1 - Jakubowski, Norbert T1 - Ink-jet application of internal standards for a better representation of tissue sections in Laser-Ablation-ICP-MS T2 - European Winter Conference on Plasma Spectrochemistry 2003 CY - Kraków, Poland DA - 2013-02-10 PY - 2013 N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. AN - OPUS4-27973 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hösl, Simone A1 - Neumann, B. A1 - Müller, Larissa A1 - Scheler, Ch. A1 - Theuring, F. A1 - Sauter, G. A1 - Schlüter, H A1 - Weller, Michael G. A1 - Linscheid, M. A1 - Jakubowski, Norbert T1 - Ink-jet application of internal standards for a better representation of tissue sections T2 - European Winter Conference on Plasma Spectrochemistry 2013 CY - Kraków, Poland DA - 2013-02-10 PY - 2013 N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. AN - OPUS4-27897 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -