TY - CONF A1 - Schwibbert, Karin A1 - Menzel, Friederike T1 - Bacterial Adhesion on Different Materials N2 - Biofilm formation on materials leads to high costs in industrial processes, as well as in medical applications. This fact has stimulated interest in the development of new materials with improved surfaces to reduce bacterial adhesion. We present a flow chamber system to test and quantify bacterial adhesion on materials that are part of antifouling concepts. The adhesion process is standardized and can be adapted to different bacteria in subaquatic of subaerial environments. It is combined with a standardized evaluation procedure based on statistical evidence. T2 - AMiCI Workshop Berlin CY - BAM Berlin, Germany DA - 07.06.2018 KW - Bacterial adhesion KW - Flow chamber system KW - Biofilm formation KW - Standardized test and quantification procedure PY - 2018 AN - OPUS4-46374 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schwibbert, Karin T1 - The Ectoine degradation pathway in Halomonas elongata T2 - Jahrestagung der VAAM (Vereinigung für Allgemeine und Angewandte Mikrobiologie) CY - Frankfurt am Main, Germany DA - 2008-03-09 PY - 2008 AN - OPUS4-17267 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schwibbert, Karin A1 - Richter, Anja A1 - Krüger, Jörg A1 - Bonse, Jörn T1 - Laser-Textured Surfaces: A Way to Control Biofilm Formation? N2 - Bacterial biofilms pose serious problems in medical and industrial settings. One of the major societal challenges lies in the increasing resistance of bacteria against biocides used in antimicrobial treatments, e.g., via overabundant use in medicine, industry, and agriculture or cleaning and disinfection in private households. Hence, new efficient bacteria-repellent strategies avoiding the use of biocides are strongly desired. One promising route to achieve bacteria-repellent surfaces lies in the contactless and aseptic large-area laser-processing of technical surfaces. Tailored surface textures, enabled by different laser-processing strategies that result in topographic scales ranging from nanometers to micrometers may provide a solution to this challenge. This article presents a current state-of-the-art review of laser-surface subtractive texturing approaches for controlling the biofilm formation for different bacterial strains and in different environments. Based on specific properties of bacteria and laser-processed surfaces, the challenges of anti-microbial surface designs are discussed, and future directions will be outlined. KW - Antibacterial surfaces KW - Biofilms KW - Laser processing KW - Laser-induced periodic surface structures (LIPSS) KW - Microbial adhesions PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-588260 DO - https://doi.org/10.1002/lpor.202300753 SN - 1863-8899 SP - 1 EP - 41 PB - Wiley-VCH GmbH CY - Weinheim AN - OPUS4-58826 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kromer, C. A1 - Schwibbert, Karin A1 - Radunz, S. A1 - Thiele, Dorothea A1 - Laux, P. A1 - Luch, A. A1 - Tschiche, H.R. T1 - ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms N2 - Bacterial biofilms can pose a serious health risk to humans and are less susceptible to antibiotics and disinfection than planktonic bacteria. Here, a novel method for biofilm eradication based on antimicrobial photodynamic therapy utilizing a nanoparticle in conjunction with a BODIPY derivative as photosensitizer was developed. Reactive oxygen species are generated upon illumination with visible light and lead to a strong, controllable and persistent eradication of both planktonic bacteria and biofilms. One of the biggest challenges in biofilm eradication is the penetration of the antimicrobial agent into the biofilm and its matrix. A biocompatible hydrophilic nanoparticle was utilized as a delivery system for the hydrophobic BODIPY dye and enabled its accumulation within the biofilm. This key feature of delivering the antimicrobial agent to the site of action where it is activated resulted in effective eradication of all tested biofilms. Here, 3 bacterial species that commonly form clinically relevant pathogenic biofilms were selected: Escherichia coli, Staphylococcus aureus and Streptococcus mutans. The development of this antimicrobial photodynamic therapy tool for biofilm eradication takes a promising step towards new methods for the much needed treatment of pathogenic biofilms. KW - Biofilm KW - Antimicrobials KW - Photodynamic therapy KW - BODIPY PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-587588 DO - https://doi.org/10.3389/fmicb.2023.1274715 SN - 1664-302X VL - 14 SP - 1 EP - 15 AN - OPUS4-58758 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Epperlein, Nadja A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Koter, Robert A1 - Bonse, Jörn A1 - Sameith, Janin A1 - Krüger, Jörg A1 - Toepel, Jörg T1 - Influence of femtosecond laser produced nanostructures on biofilm growth on steel N2 - Biofilm formation poses high risks in multiple industrial and medical settings. However, the robust nature of biofilms makes them also attractive for industrial applications where cell biocatalysts are increasingly in use. Since tailoring material properties that affect bacterial growth or its inhibition is gaining attention, here we focus on the effects of femtosecond laser produced nanostructures on bacterial adhesion. Large area periodic surface structures were generated on steel surfaces using 30-fs laser pulses at 790 nm wavelength. Two types of steel exhibiting a different corrosion resistance were used, i.e., a plain structural steel (corrodible) and a stainless steel (resistant to corrosion). Homogeneous fields of laser-induced periodic surface structures (LIPSS) were realized utilizing laser fluences close to the ablation threshold while scanning the sample under the focused laser beam in a multi-pulse regime. The nanostructures were characterized with optical and scanning electron microscopy. For each type of steel, more than ten identical samples were laser-processed. Subsequently, the samples were subjected to microbial adhesion tests. Bacteria of different shape and adhesion behavior (Escherichia coli and Staphylococcus aureus) were exposed to laser structures and to polished reference surfaces. Our results indicate that E. coli preferentially avoids adhesion to the LIPSS-covered areas, whereas S. aureus favors these areas for colonization. KW - Laser-induced periodic surface structures KW - Femtosecond laser KW - Steel KW - Biofilms KW - Microbial adhesion tests PY - 2017 UR - http://www.sciencedirect.com/science/article/pii/S0169433217305470 DO - https://doi.org/10.1016/j.apsusc.2017.02.174 SN - 0169-4332 SN - 1873-5584 VL - 418 IS - Part B SP - 420 EP - 424 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-40565 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Dietrich, P. A1 - Beblo-Vranesevic, K. A1 - Kjærvik, Marit A1 - Unger, Wolfgang A1 - Schwibbert, Karin A1 - Hardie, K. A1 - Brown, J. T1 - XPS surface analysis of bacterial samples N2 - This application note presents how EnviroESCA can be used to analyze bacterial samples under near ambient pressure conditions in various states of hydration using different levels of humidity. Such investigations of bacterial cell wall surfaces in their hydrated state are essential for studying biological interfaces at work. The use of innovative near-ambient pressure (NAP-)XPS instrumentation allows the detailed analysis of irregularly-surfaced biofilms. NAP-XPS enables the surface analysis of bacterial samples in their natural hydrated state without complex sample preparation techniques such as freeze-drying or fast-freezing, which are needed for XPS analysis in ultrahigh vacuum. KW - Near ambient pressure XPS KW - Biofilms PY - 2018 UR - http://www.enviroai.com/uploads/1/0/2/8/102861712/xps_surface_analysis_of_bacillus_subtilis_biofilms_final.pdf IS - Application Note #000399 SP - 1 EP - 5 CY - Berlin AN - OPUS4-44588 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Kjaervik, Marit A1 - Schwibbert, Karin A1 - Dietrich, P. A1 - Thissen, A. A1 - Unger, Wolfgang T1 - Near-ambient pressure XPS of hydrated Escherichia coli samples with EnviroESCA N2 - This application note presents how EnviroESCA can be used to analyze E. coli biofilms on silicon under near ambient pressure conditions in various states of hydration. Such investigations of the outer bacterial cell surface in their hydrated state are essential for studying biological interfaces at work. KW - Biofilms KW - E. coli KW - NAP-XPS PY - 2018 UR - http://www.enviroai.com/uploads/1/0/2/8/102861712/nap_xps_of_escherichia_coli_samples.pdf IS - Application Note #000400 SP - 1 EP - 4 CY - Berlin, Germany AN - OPUS4-45720 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hoffmann, Heike A1 - Schwibbert, Karin ED - Annokkée, G. J. T1 - Monitoring the influence of biocides on soil bacteria using fluorescence microscopy T2 - 8th International FZK/TNO Conference on Contaminated Soil ; 8th ConSoil 2003 CY - Gent, Belgium DA - 2003-05-12 PY - 2003 SN - 0947-8620 N1 - Geburtsname von Schwibbert, Karin: Göller, K. - Birth name of Schwibbert, Karin: Göller, K. N1 - Serientitel: Wissenschaftliche Berichte / Forschungszentrum Karlsruhe in der Helmholtz-Gemeinschaft – Series title: Wissenschaftliche Berichte / Forschungszentrum Karlsruhe in der Helmholtz-Gemeinschaft IS - 6943 SP - 1417 EP - 1423 PB - Forschungszentrum CY - Karlsruhe AN - OPUS4-2539 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schwibbert, Karin A1 - Rudolph, Dieter T1 - The Need for Unequivocally Defined Reference Fungi - Genomic Variation in Two Strains Named as Coniophora puteana BAM Ebw. 15 KW - European standard EN 113 KW - Reference fungi KW - Coniophora puteana KW - RAPD analysis KW - DNA patterns PY - 2003 SN - 0018-3830 SN - 1437-434X N1 - Geburtsname von Schwibbert, Karin: Göller, K. - Birth name of Schwibbert, Karin: Göller, K. VL - 57 IS - 5 SP - 456 EP - 458 PB - De Gruyter CY - Berlin AN - OPUS4-2651 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kjaervik, Marit A1 - Schwibbert, Karin A1 - Dietrich, P.M. A1 - Unger, Wolfgang T1 - NAP-XPS spectra of the bacterial cell-envelope of Pseudomonas fluorescens bacteria N2 - Pseudomonas fluorescens (Gram-negative) bacteria purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures were analyzed using high-resolution x-ray photoelectron spectroscopy at near ambient pressure conditions (NAP-XPS), 1500 Pa water vapor atmosphere. Fresh layers of P. fluorescence bacteria were grown on Luria Broth agar plates. Bacteria were taken from the agar plate with a sterile spatula and gently spread on a Si-wafer piece for NAP-XPS analysis. The NAP-XPS spectra of the bacterial envelope of P. fluorescence were obtained using monochromatic Al Kα radiation and include a survey scan and high-resolution spectra of C 1s, N 1s, P 2p, and O 1s as well. The presentation of the C 1s high-resolution spectrum includes the results of peak fitting analysis. KW - Pseudomonas fluorescens KW - Cell-envelope KW - Water atmosphere KW - Near ambient x-ray photoelectron spectroscopy KW - NAP-XPS PY - 2022 DO - https://doi.org/10.1116/6.0001543 SN - 1055-5269 VL - 29 IS - 1 SP - 014008-1 PB - AVS AN - OPUS4-54464 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bonse, Jörn A1 - Schwibbert, Karin T1 - Laserstrukturierte Oberflächen: Ein Weg zur Kontrolle der Biofilmbildung? N2 - Bakterielle Biofilme stellen in medizinischen und industriellen Bereichen ein ernsthaftes Problem dar. Eine der größten gesellschaftlichen Herausforderungen liegt in der zunehmenden Resistenz von Bakterien gegen Biozide, die bei antimikrobiellen Behandlungen eingesetzt werden, z.B. durch übermäßigen Einsatz in Medizin, Industrie und Landwirtschaft oder durch Reinigung und Desinfektion in Privathaushalten. Daher sind neue effiziente bakterienabweisende Strategien, die den Einsatz von Bioziden vermeiden, dringend erforderlich. Ein vielversprechender Weg zur Erzielung bakterienabweisender Oberflächen liegt in der berührungslosen und aseptischen großflächigen Laserbearbeitung von technischen Oberflächen. Maßgeschneiderte Oberflächentexturen, ermöglicht durch verschiedene Laserbearbeitungsstrategien, die zu topographischen Skalen im Bereich von Nanometern bis Mikrometern führen, können eine Lösung für diese Herausforderung darstellen. In dem Vortrag wird ein Überblick über den aktuellen Stand der Technik bei der subtraktiven Texturierung von Laseroberflächen zur Kontrolle der Biofilmbildung bei verschiedenen Bakterienstämmen und in unterschiedlichen Umgebungen gegeben. Auf der Grundlage spezifischer Eigenschaften von Bakterien und laserbearbeiteten Oberflächen werden die Herausforderungen antimikrobieller Oberflächendesigns erörtert und zukünftige Richtungen aufgezeigt. T2 - GRAVOSeminar, GRAVOmer Kompetenznetzwerk CY - Online meeting DA - 28.02.2024 KW - Laser-Materialbearbeitung KW - Ultrakurzpuls-Laser KW - Biofilme KW - Antibakterielle Oberflächen KW - Oberflächenfunktionalisierung PY - 2024 UR - https://gravomer.de/veranstaltungen-anzeigen/laserstrukturierte-oberflaechen-ein-weg-zur-kontrolle-der-biofilmbildung AN - OPUS4-59594 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -