TY - JOUR A1 - Schneider, Thomas A1 - Vierneusel, B. A1 - Gradt, Thomas A1 - Tremmel, S. A1 - Wartzack, S. A1 - Szücs, Z. A1 - Arendt, I. T1 - Optimierte MoS2-PVD-Schichten für wechselnde Umgebungsbedingungen N2 - Der Festschmierstoff Molybdändisulfid ist insbesondere für Extremanwendungen ein gerne eingesetzter Werkstoff, problematisch ist jedoch der negative Einfluss von Feuchtigkeit. Bei mittels PVD-Verfahren abgeschiedenen MoS2-Schichten ergibt sich jedoch die Möglichkeit, durch Anpassung der Beschichtungsparameter vorwiegend basal ausgerichtete Schichten herzustellen, die auch an Luft ein verbessertes tribologisches Verhalten zeigen. N2 - The solid lubricant molybdenum disulfide is a well known material for extreme conditions, but Problems arise due to a negative effect of humidity. However, by using a PVD process, deposition parameters can be optimized to gain coatings with a more textured structure orienting the basal planes parallel to the Substrate surface. Therefore, an improved tribological behavior even under moist conditions can be achievcd. KW - MoS2-coatings KW - PVD-process KW - Tribology KW - Moisture KW - Cryogenic temperatures KW - MoS2-Beschichtungen KW - PVD KW - Tribologie KW - Luftfeuchte KW - Kryogene Temperaturen PY - 2013 SN - 0724-3472 VL - 60 IS - 4 SP - 18 EP - 23 PB - Expert Verlag CY - Renningen AN - OPUS4-29995 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Fernández, L. A1 - Lin, Z. A1 - Schneider, Rudolf A1 - Esteves, V. I. A1 - Cunha, Â. A1 - Tomé, J. P. C. T1 - Antimicrobial Photodynamic Activity of Cationic Nanoparticles Decorated with Glycosylated Photosensitizers for Water Disinfection N2 - The antimicrobial photodynamic approach has been demonstrated as an efficient and sustainable process for the eradication of microbial pathogens. In this work, silica-coated Magnetite nanoparticles (NPs) were used as carriers of glycosylated porphyrins and phthalocyanines. Their subsequent cationization resulted in the production of stable antimicrobial photosensitizing materials, effective against E. coli. Suspensions of the photocatalysts in water present bimodal size distributions formed by big clusters and small NPs with hydrodynamic diameters between 8 and 38 nm. The presence of small NPs in the suspensions is related to an effective photodynamic inactivation (PDI) of E. coli cells. Glycosylation of the PS showed a positive effect on the PDI performance, which could be related to a higher accumulation of the photocatalyst over the bacterial cell membrane. In addition, these biocidal agents proved to be photostable and their photoactive performance decreased only between 23% and 28% upon 5 PDI cycles, mostly because of the loss of material between cycles, which makes them promising materials for water disinfection purposes. KW - Photodynamische Inaktivierung KW - E. coli KW - Photokatalyse KW - Bakterien KW - Nanopartikel KW - Photosensibilisator KW - Porphyrin KW - Phthalocyanin PY - 2018 DO - https://doi.org/10.1002/cptc.201700169 SN - 2367-0932 VL - 2 IS - 7 SP - 596 EP - 605 PB - Wiley VHC CY - Weinheim AN - OPUS4-45684 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hille, Falk A1 - Herrmann, Ralf A1 - Schneider, Ronald A1 - Pitters, S. A1 - Wedel, F. A1 - Hindersmann, I. T1 - Developing a guideline for structural health monitoring of road bridges in Germany N2 - In recent years, Structural Health Monitoring (SHM) has become a useful and increasingly widely used tool for supporting lifetime extensions of existing bridges with known structural deficiencies or indications of potentially critical damages or damage processes. At the same time, methods and tools are emerging, which enable monitoring-informed predictive maintenance of new and existing bridges based on digital twins. The monitoring process – starting from the definition of monitoring actions and ending with decisions based on monitoring outcomes – is complex and requires expertise in structural engineering, operation and maintenance of bridges, metrology, and data analytics. To support German road authorities, engineering consultancies, building contractors and other stakeholders of the bridge management, the Federal Highway Research Institute (BASt) has initiated the development of a new guideline for applying SHM as part of the management of road bridges. The guideline will present various use cases and for each identified use case, it will propose a proven monitoring scheme. In addition, the guideline will provide guidance on assessing the benefits of SHM as well as a common approach to managing monitoring data as a systematic basis for integrating monitoring data in the bridge management. This contribution discusses the motivation, objectives, and scope of the guideline, describes its use case centric structure and outlines the proposed data management. T2 - IABMAS 2024 CY - Kopenhagen, Danmark DA - 24.06.2024 KW - Guideline KW - Structural health monitoring KW - Road bridges KW - Infrastructure PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-613947 SN - 978-1-032-77040-6 DO - https://doi.org/10.1201/9781003483755-236 SP - 2009 EP - 2017 PB - CRC Press AN - OPUS4-61394 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Wedel, F. A1 - Pitters, S. A1 - Herrmann, Ralf A1 - Schneider, Ronald A1 - Hille, Falk A1 - Hindersmann, I. T1 - Guideline for the strategic application of monitoring of road bridges in Germany N2 - Engineering structures are an important part of our transport infrastructure. Their failure is associated with high safety risks and economic damage. Ensuring the availability of these constructions and guaranteeing their operational safety are thus important tasks. Currently, maintenance of engineering structures is a reactive process and therefore not yet a predictive process. Every 3 to 6 years, the structures are visually inspected, all damages are documented and a condition grade is assigned to the structure based on the detected damages. Continuous data-providing methods such as monitoring are used only very occasionally. With the help of monitoring, condition data about the structures are continuously collected, which can be used for a better assessment of the structures. On the one hand, this would increase safety of engineering structures, and on the other hand, it makes the shift towards predictive maintenance management based on real-time data and predictions, where the onset of damage is detected before it occurs, possible in the first place. Monitoring plays therefore an important role in the management of the infrastructure. It is a crucial a step towards the digitalisation of our infrastructure and existing processes. Although there are many use cases for monitoring that have already been successfully implemented, monitoring is not yet widely used by German road authorities due to several challenges that were also identified within the scope of the project. Therefore, a guide has been developed in which the current obstacles related to monitoring are identified and possibilities for integrating monitoring into existing processes are offered. To ensure optimal alignment with the needs of road authorities, both an online survey and a workshop have been conducted. The results are summarised in a brochure that is made available to authorities to improve the use of monitoring in Germany. This paper presents the guideline and the brochure. T2 - 10th European Workshop on Structural Health Monitoring (EWSHM 2024) CY - Potsdam, Germany DA - 10.06.2024 KW - Verkehrsinfrastrukturen KW - Engineering Structures KW - Bridges KW - Structural Health Monitoring KW - Guideline PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-612821 UR - https://www.ndt.net/search/docs.php3?id=29582 DO - https://doi.org/10.58286/29582 SN - 1435-4934 VL - 29 IS - 7 SP - 1 EP - 8 PB - NDT.net GmbH & Co. KG CY - Mayen AN - OPUS4-61282 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Herrmann, Ralf A1 - Hille, Falk A1 - Pitters, S. A1 - Ramasetti, Eshwar Kumar A1 - Schneider, Ronald A1 - Wedel, F. A1 - Hindersmann, I. ED - Rogge, Andreas ED - Meng, Birgit T1 - Föderiertes Datenmanagement von Monitoringdaten aus Structural Health Monitoring Anwendungen und daraus gewonnenen Trainingsdaten bei Spannbetonbrücken N2 - Beim Structural Health Monitoring entstehen eine Vielzahl von Daten, Metadaten und Modellen mit hohem Wert für die Beurteilung des Bauwerkszustands und der Prognose von Veränderungen. Kontinuierlich anwachsende Datenbestände müssen nachhaltig geteilt, genutzt und über die Bauwerkslebensdauer archiviert werden. Dabei stellen die Vielfalt der Messaufgaben, die Heterogenität der Daten, die dezentrale Erfassung und z. T. der Umfang eine Herausforderung für die beteiligten Akteure dar. Für den Datentransfer der Monitoringdaten zwischen der Straßenbauverwaltung und den Monitoringausführenden wird ein flexibles Abstimmungsverfahren vorgeschlagen und ein Lösungskonzept für ein föderiertes Datenmanagement skizziert. Eine weitere Herausforderung stellt die bauwerksübergreifende Zusammenstellung von Trainingsdaten für konkrete KI-Anwendungen dar. T2 - 11. Jahrestagung des DAfStb mit 63. Forschungskolloquium der BAM Green Intelligent Building CY - Berlin, Germany DA - 16.10.2024 KW - Structural Health Monitoring PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-612902 SN - 978-3-9818564-7-7 SP - 178 EP - 185 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-61290 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hille, Falk A1 - Herrmann, Ralf A1 - Pitters, S. A1 - Hindersmann, I. A1 - Schneider, Ronald A1 - Wedel, F. ED - Rogge, Andreas ED - Meng, Birgit T1 - Leitfaden für den strategischen Einsatz von Monitoring an Straßenbrücken N2 - Für die Anwendung von Structural Health Monitoring (SHM) im Rahmen des Managements von Straßenbrü-cken wurde zur Unterstützung von Straßenbauverwaltungen, Ingenieurbüros, Bauunternehmen und weiteren Akteuren die Erstellung eines Leitfadens initiiert. Dieser Leitfaden stellt typische Anwendungsfälle für Monito-ring vor und schlägt für diese bewährte Herangehensweisen vor. Darüber hinaus bietet der Leitfaden Empfeh-lungen für Ausschreibung und Vergabe von Monitoringleistungen, eine Anleitung zur Bewertung des wirt-schaftlichen Nutzens von SHM, Hinweise zur optimal einsetzbaren Messtechnik sowie einen Ansatz für das Datenmanagement zur Integration der Monitoringdaten in das Brückenmanagement. T2 - 11. Jahrestagung des DAfStb mit 63. Forschungskolloquium der BAM Green Intelligent Building CY - Berlin, Germany DA - 16.10.2024 KW - Leitfaden KW - Monitoring KW - Straßenbrücke PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-612931 SN - 978-3-9818564-7-7 SP - 186 EP - 191 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-61293 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramos, Inês I. A1 - Carl, Peter A1 - Schneider, Rudolf A1 - Segundo, Marcela A. T1 - Development of automated immunosensing methods for clinical and environmental Analysis using lab-on-valve platforms N2 - The lab-on-valve (LOV) is a mesofluidic platform that has been recently exploited for the automation and miniaturization of bioanalytical assays, resorting namely to molecular recognition schemes based on immunosensing. Due to its high versatility for reagent accommodation, it is possible to establish immunoassays under several formats (eg. direct competitive ELISA, sandwich ELISA or even label-free immunoaffinity chromatography). For instance, the LOV has been used as a manifold for UV-vis micro-Bead Injection Spectroscopy (μ-BIS), a technique that involves the quantification of the target analyte by direct measurement on the surface of a solid phase capable of retaining the target analyte by molecular recognition. The μ-BIS-LOV strategy affords several analytical advantages, namely short time-toresult intervals (3 to 15 min), low sample volume (1-20 μL), automated solution handling and washing steps, downscaling of reagents’ consumption, low-cost analysis and little generation of waste. Additionally, the solid support is renewed before each determination, minimizing surface fouling, cross-contamination issues and functional group deactivation. No sample clean-up steps are required because interferences are separated from the target analyte upon quantification mediated by a molecular recognition element attached to the micro-bead column. The portability of the LOV device makes it compatible with point-of-care testing. To our knowledge, this technique has been mainly employed for the evaluation and optimization of bioaffinity processes, but its potential for clinical and environmental analysis remains underexploited. Hence, in this communication, different immunosensing strategies using the LOV platform will be addressed, namely the determination of autoimmune IgG in human serum, and the assessment of drug (carbamazepine) levels in wastewater samples. T2 - EBS 2017 CY - Potsdam, Germany DA - 20.03.2017 KW - Lab-on-valve KW - Automation KW - Immunoassay PY - 2017 AN - OPUS4-39521 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Thomas A1 - Vierneusel, B. A1 - Gradt, Thomas A1 - Tremmel, S. A1 - Wartzack, S. A1 - Szücs, Z. A1 - Arendt, I. T1 - Optimierte MoS2-PVD-Schichten für wechselnde Umgebungsbedingungen N2 - Der Festschmierstoff Molybdändisulfid ist insbesondere für Extremanwendungen, bei denen konventionelle Öl- oder Fettschmierung nicht in Frage kommen (Vakuum, hohe Pressungen) ein gerne eingesetzter Werkstoff. Problematisch ist jedoch der negative Einfluss von Feuchtigkeit, wodurch die Einsetzbarkeit von M0S2 unter Normalbedingungen leidet. Bei mittels PVD-Verfahren abgeschiedenen MoSa-Schichten ergibt sich jedoch die Möglichkeit, durch Anpassung der Beschichtungsparameter vorwiegend basal ausgerichtete Schichten herzustellen, die auch an normal feuchter Luft ein verbessertes tribologisches Verhalten zeigen. MoSa-Schichten sind darüber hinaus auch für Anwendungen bei extrem tiefen Temperaturen interessant, da hier kein Schmierstoff zur Verfügung steht, der ähnlich günstige Reibeigenschaften aufweist. Für bewegliche Stützelemente zwischen supraleitenden Magnetspulen des Kernfusions-Experiments Wendelstein 7-X waren bis zu 7 pm dicke Schichten entwickelt worden, die jedoch durch das notwendige langsame Aufwachsen für andere Anwendungen nicht wirtschaftlich herzustellen waren. Hier sollen neue, im Rahmen eines Kooperationsprojekts entstandene MoS2-PVD-Schichten vorgestellt werden, die bei wechselnden Umgebungsbedingungen noch gute tribologische Eigenschaften liefern, jedoch durch ihre Dicke von 1-2 pm und hoher Aufwachsrate kostengünstig sind. N2 - The solid lubricant molybdenum disulfide is a well known material for extreme conditions where conventlonal oll or grease lubrication is not feasible (vacuum, high pressures). Problems arise due to a negative effect of humidity which is a drawback for the suitability of M0S2 under normal conditions. However, by using a PVD process, Deposition parameters can be optimized to gain coatings with a more textured structure orienting the basal planes parallel to the Substrate surface. Therefore, an improved tribological behavior even under moist conditions can be achieved. MoS2-coatings additionally are interesting candidates for being applied under cryogenic temperatures, since no lubricant is available having similarly favorable frictional properties. Up to 7 pm thick coatings have been developed especially for sliding support elements between superconducting magnets of the fusion-experiment Wendelstein 7-X, however, the extremely slow deposition rate precluded its utilization on more common tribo-systems due to cost considerations. Here, new MoS2-coatings are presented which were developed in a cooperational project, giving good tribological results under variing environments but are more cost effective being only 1-2 pm in thickness and faster in film growth.. T2 - 53. Tribologie-Fachtagung - Reibung, Schmierung und Verschleiß CY - Göttingen, Germany DA - 24.09.2012 PY - 2012 SN - 978-3-00-039201-6 SP - 46/1 EP - 46/12 CY - Aachen AN - OPUS4-26724 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Mi, W. A1 - Josephs, R. D. A1 - Melanson, J. E. A1 - Dai, X. A1 - Wang, Y. A1 - Zhai, R. A1 - Chu, Z. A1 - Fang, X. A1 - Thibeault, M.-P. A1 - Stocks, B. B. A1 - Meija, J. A1 - Bedu, M. A1 - Martos, G. A1 - Westwood, S. A1 - Wielgosz, R. I. A1 - Liu, Q. A1 - Teo, T. L. A1 - Liu, H. A1 - Tan, Y. J. A1 - Öztuğ, M. A1 - Saban, E. A1 - Kinumi, T. A1 - Saikusa, K. A1 - Schneider, Rudolf A1 - Weller, Michael G. A1 - Konthur, Zoltán A1 - Jaeger, Carsten A1 - Quaglia, M. A1 - Mussell, C. A1 - Drinkwater, G. A1 - Giangrande, C. A1 - Vaneeckhoutte, H. A1 - Boeuf, A. A1 - Delatour, V. A1 - Lee, J. E. A1 - O'Connor, G. A1 - Ohlendorf, R. A1 - Henrion, A. A1 - Beltrão, P. J. A1 - Naressi Scapin, S. M. A1 - Sade, Y. B. T1 - PAWG Pilot Study on Quantification of SARS-CoV-2 Monoclonal Antibody - Part 1 N2 - Under the auspices of the Protein Analysis Working Group (PAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by the Chinese National Institute of Metrology (NIM), National Research Council of Canada (NRC) and the Bureau International des Poids et Mesures (BIPM). Eleven Metrology Institutes or Designated Institutes and the BIPM participated in the first phase of the pilot study (Part 1). The purpose of this pilot study was to develop measurement capabilities for larger proteins using a recombinant humanized IgG monoclonal antibody against Spike glycoprotein of SARS-CoV-2 (Anti-S IgG mAb) in solution. The first phase of the study was designed to employ established methods that had been previously studies by the CCQM Protein Analysis Working Group, involving the digestion of protein down to the peptide or amino acid level. The global coronavirus pandemic has also led to increased focus on antibody quantitation methods. IgG are among the immunoglobulins produced by the immune system to provide protection against SARS-CoV-2. Anti-SARS-CoV-2 IgG can therefore be detected in samples from affected patients. Antibody tests can show whether a person has been exposed to the SARS-CoV-2, and whether or not they potentially show lasting immunity to the disease. With the constant spread of the virus and the high pressure of re-opening economies, antibody testing plays a critical role in the fight against COVID-19 by helping healthcare professionals to identify individuals who have developed an immune response, either via vaccination or exposure to the virus. Many countries have launched large-scale antibody testing for COVID-19. The development of measurement standards for the antibody detection of SARS-CoV-2 is critically important to deal with the challenges of the COVID-19 pandemic. In this study, the SARS-CoV-2 monoclonal antibody is being used as a model system to build capacity in methods that can be used in antibody quantification. Amino acid reference values with corresponding expanded uncertainty of 36.10 ± 1.55 mg/kg, 38.75 ± 1.45 mg/kg, 18.46 ± 0.78 mg/kg, 16.20 ± 0.67 mg/kg and 30.61 ± 1.30 mg/kg have been established for leucine, valine, phenylalanine, isoleucine and proline, respectively. Agreement between nearly all laboratories was achieved for the amino acid analysis within 2 to 2.5 %, with one participant achieving markedly higher results due to a technical issue found in their procedure; this result was thus excluded from the reference value calculations. The relatively good agreement within a laboratory between different amino acids was not dissimilar to previous results for peptides or small proteins, indicating that factors such as hydrolysis conditions and calibration procedures could be the largest sources of variability. Peptide reference values with corresponding expanded uncertainty of 4.99 ± 0.28 mg/kg and 6.83 ± 0.65 mg/kg have been established for ALPAPIEK and GPSVFPLAPSSK, respectively. Not surprisingly due to prior knowledge from previous studies on peptide quantitation, agreement between laboratories for the peptide-based analysis was slightly poorer at 3 to 5 %, with one laboratory's result excluded for the peptide GPSVFPLAPSSK. Again, this level of agreement was not significantly poorer than that achieved in previous studies with smaller or less complex proteins. To reach the main text of this paper, click on Final Report. KW - Antibody quantification KW - Amino acid analysis KW - Peptide analysis KW - Round robin test PY - 2021 DO - https://doi.org/10.1088/0026-1394/59/1a/08001 VL - 59 IS - 1A SP - 08001 AN - OPUS4-54972 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hoffmann, Holger A1 - Baldofski, Stefanie A1 - Hoffmann, Kristin A1 - Flemig, Sabine A1 - Silva, C. P. A1 - Esteves, V. I. A1 - Emmerling, Franziska A1 - Panne, Ulrich A1 - Schneider, Rudolf T1 - Structural considerations on the selectivity of an immunoassay for sulfamethoxazole N2 - Sulfamethoxazol (SMX),a sulfonamide, is a widely used bacteriostatic antibiotic and therefore a promising marker for the entry of anthropogenic Pollution in the environment. SMX is frequently found in wastewater and surface water. This study presents the production of high affinity and selective polyclonal antibodies for SMX and the development and Evaluation of a direct competitive enzyme-linked immunosorbent assay(ELISA)for the quantification of SMX in environmental watersamples. The crystal structures of the cross-reacting compounds sulfamethizole, N4-acetyl-SMX andsuccinimidyl-SMX were determined by x-ray diffraction aiming to explain their high cross-reactivity. These crystal structures are described for the first time. The quantification range of the ELISA is 0.82–63 µg/L. To verify our results, the SMX concentration in 20 environmental samples,including wastewater and surfacewater,was determined by ELISA and tandem mass spectrometry(MS/MS).A good Agreement of the measured SMX concentrations was found with average recoveries of 97–113%for the results of ELISA compared to LC-MS/MS. KW - X-Ray diffraction KW - ELISA KW - LC-MS/MS KW - Sulfamethoxazole PY - 2016 DO - https://doi.org/10.1016/j.talanta.2016.05.049 SN - 0039-9140 SN - 1873-3573 IS - 158 SP - 198 EP - 207 PB - Elsevier B.V. CY - Amsterdam, Netherlands AN - OPUS4-38530 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -