TY - CONF A1 - Schloß, Svenja A1 - Proske, Matthias A1 - Zaspel, I. A1 - Koch, Matthias A1 - Maul, Ronald T1 - Rapid Preparation of 15N(3)-labeled Enniatins T2 - JCF-Frühjahrssymposium 2013 CY - Berlin, Germany DA - 2013-03-06 PY - 2013 AN - OPUS4-27865 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Proske, Matthias A1 - Maul, Ronald A1 - Welz, C. A1 - Zaspel, I. T1 - Wood inhabiting endophytic fungi as producers of bioactive metabolites T2 - Plant Protection and Plant Health in Europe 2013 CY - Berlin, Germany DA - 2013-05-27 PY - 2013 AN - OPUS4-28578 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Koch, Matthias A1 - Maul, Ronald A1 - Proske, Matthias T1 - Gewinnung und Charakterisierung von Enniatinen aus Pilzkulturen T2 - 41. Deutscher Lebensmittelchemikertag 2012 CY - Münster, Germany DA - 2012-09-10 PY - 2012 AN - OPUS4-26495 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Kutscha, Sandra A1 - Koch, Matthias A1 - Proske, Matthias A1 - Maul, Ronald T1 - Development of a HPLC-MS-Method for the Quantification of Enniatin B in Fermented Feed T2 - 42. Deutscher Lebensmittelchemikertag 2013 CY - Braunschweig, Germany DA - 2013-09-16 PY - 2013 AN - OPUS4-29170 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Wedell, Ines A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Neue Trends & Alte Probleme: Das Leguminosenmykotoxin Phomopsin A in einer "worst case"-Betrachtung N2 - Leguminosen wie z.B. Bohnen, Erbsen, Soja und zunehmend auch die Samen der Süßlupine liefern aufgrund ihres hohen Proteingehaltes einen wertvollen Beitrag zu einer veganen oder vegetarischen Ernährung. Jedoch sind Lupinen anfällig für Infektionen mit dem Pilz Diaporthe toxica, der u.a. Phomopsin A als hepatotoxischen Sekundärmetaboliten bildet. Ziel der Arbeiten waren die Gewinnung und Anwendung eines 15N-isotopenmarkierten internen Standards für die HPLC-MS/MS-Analyse dieses Toxins sowie die Quantifizierung der Toxinbildung in einem „worst case“-Szenario. T2 - 50. DGQ-Vortragstagung (Deutsche Gesellschaft für Qualitätsforschung e. V.) CY - Berlin, Germany DA - 14.03.2026 KW - Phomopsin A KW - Leguminosen KW - SIDA-HPLC-MS/MS KW - Quantifizierung KW - Toxinbelastung PY - 2016 AN - OPUS4-37059 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Wedell, Ines A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Sensitive und selektive Untersuchung des Leguminosenmykotoxins Phomopsin A - eine "worst case"-Betrachtung N2 - Leguminosen wie z.B. Bohnen, Erbsen, Soja und zunehmend auch die Samen der Süßlupine liefern aufgrund ihres hohen Proteingehaltes einen wertvollen Beitrag zu einer veganen oder vegetarischen Ernährung. Jedoch sind Lupinen anfällig für Infektionen mit dem Pilz Diaporthe toxica, der u.a. Phomopsin A als hepatotoxischen Sekundärmetaboliten bildet. Ziel der Arbeiten waren die Gewinnung und Anwendung eines 15N-isotopenmarkierten internen Standards für die HPLC-MS/MS-Analyse dieses Toxins sowie die Quantifizierung der Toxinbildung in einem „worst case“-Szenario. T2 - 53. Wissenschaftlicher Kongress der Deutschen Gesellschaft für Ernährung e.V. CY - Fulda, Germany DA - 02.03.2016 KW - Phomopsin A KW - Leguminosen KW - Stabilisotopenverdünnungsanalyse KW - HPLC-MS/MS KW - Toxinbelastung PY - 2016 AN - OPUS4-37060 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Wedell, Ines A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Die Bestimmung von Phomopsin A in Leguminosen mittels Stabilisotopenverdünnungsanalyse T2 - GDCh-Regionalverbandstagung Nord CY - Oldenburg DA - 2015-03-09 PY - 2015 AN - OPUS4-32442 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Wedell, Ines A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Die Bestimmung von Phomopsin A in Leguminosen mittels Stabilisotopenverdünnungsanalyse T2 - GDCh-Regionalverbandstagung Nordost CY - Berlin DA - 2015-03-16 PY - 2015 AN - OPUS4-32443 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schloß, Svenja A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, R. T1 - Development of a SIDA-LC-MS/MS method for the determination of phomopsin A in legumes N2 - A novel method for the determination of phomopsin A (1) in lupin flour, pea flour, and bean flour as well as whole lupin plants was established based on stable isotope dilution assay (SIDA) LC-MS/MS using 15N6-1 as an isotopically labeled internal standard. Artificially infected samples were used to develop an optimized extraction procedure and sample pretreatment. The limits of detection were 0.5–1 µg/kg for all matrices. The limits of quantitation were 2–4 µg/kg. The method was used to analyze flour samples generated from selected legume seeds and lupin plant samples that had been inoculated with Diaporthe toxica and two further fungal strains. Finally, growing lupin plants infected with D. toxica were investigated to simulate a naturally in-field mycotoxicosis. Toxin levels of up to 10.1 µg/kg of 1 were found in the pods and 7.2 µg/kg in the stems and leaves. KW - Diaporthe toxica KW - Stable isotope dilution assay KW - Isotopic labeled internal standard KW - Legume contamination PY - 2015 DO - https://doi.org/10.1021/acs.jafc.5b04792 SN - 0021-8561 SN - 1520-5118 VL - 63 IS - 48 SP - 10543 EP - 10549 PB - American Chemical Society CY - Columbus, Ohio AN - OPUS4-35071 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schloß, Svenja A1 - Wedell, Ines A1 - Koch, Matthias A1 - Rohn, S. A1 - Maul, Ronald T1 - Biosynthesis and characterization of 15N6-labeled phomopsin A, a lupin associated mycotoxin produced by Diaporthe toxica N2 - The hepatotoxin phomopsin A (PHO-A), a secondary metabolite mainly produced by the fungus Diaporthe toxica, occurs predominantly on sweet lupins. Along with the growing interest in sweet lupins for food and feed commodities, concerns have been raised about fungal infestations, and consequently, about the determination of PHO-A. High performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) represents the most suitable analytical technique for sensitive and selective detection of mycotoxins including PHO-A. However, isotopic labeled substances are needed as internal standards for a reliable and convenient quantification. As no isotope standard for PHO-A is currently available, a biosynthesis of fully 15N6-labeled PHO-A was established by cultivation of D. toxica on defined media containing Na15NO3 and 15N-labeled yeast extract as the only nitrogen sources. The identity of 15N6-PHO-A was confirmed by high resolution mass spectrometry. The new 15N6-labeled standard will facilitate the method development for PHO-A including a more accurate quantification by LC-MS/MS. KW - Phomopsin A KW - Lupins KW - Diaporthe toxica KW - Stable isotope dilution assay KW - Isotopic labeled internal standard KW - Biosynthesis PY - 2015 DO - https://doi.org/10.1016/j.foodchem.2014.12.056 SN - 0308-8146 VL - 177 SP - 61 EP - 65 PB - Elsevier CY - Amsterdam [u.a.] ; Jena AN - OPUS4-32464 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Wedell, I. A1 - Koch, Matthias A1 - Maul, Ronald T1 - Quantification of Phomopsin A: Biosynthesis of 15N6-labeled Internal Standard T2 - Rapid Methods Europe 2014 CY - Noordwijkerhout, Netherlands DA - 2014-03-31 PY - 2014 AN - OPUS4-31128 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schloß, Svenja A1 - Proske, Matthias A1 - Zaspel, I. A1 - Koch, Matthias A1 - Maul, Ronald A1 - Panne, Ulrich T1 - Isolation and Characterization of Enniatins from Fungal Cultures T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-27864 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -