TY - CONF A1 - Thöns, S. A1 - Schneider, Ronald A1 - Faber, M.H. ED - Haukaas, T. T1 - Quantification of the value of structural health monitoring information for fatigue deteriorating structural systems N2 - This paper addresses the quantification of the value of structural health monitoring (SHM) before its implementation for structural systems on the basis of its Value of Information (VoI). The value of SHM is calculated utilizing the Bayesian pre-posterior decision analysis modelling the structural life cycle performance, the integrity management and the structural risks. The relevance and precision of SHM information for the reduction of the structural system risks and the expected cost of the structural integrity management throughout the life cycle constitutes the value of SHM and is quantified with this framework. The approach is focused on fatigue deteriorating structural steel systems for which a continuous resistance deterioration formulation is introduced. In a case study, the value of SHM for load monitoring is calculated for a Daniels system subjected to fatigue deterioration. The influence of and the value of SHM in regard to the structural system risks and the integrity management is explicated and explained. The results are pointing to the importance of the consideration of the structural system risks for the quantification of the value of SHM. T2 - ICASP 12 - 12th International conference on applications of statistics and probability in civil engineering CY - Vancouver, Canada DA - 12.07.2015 PY - 2015 DO - https://doi.org/10.14288/1.0076284 SP - Paper 605, 1 EP - 8 AN - OPUS4-34332 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Ronald A1 - Fischer, J. A1 - Bügler, M. A1 - Nowak, M. A1 - Thöns, S. A1 - Borrmann, A. A1 - Straub, D. T1 - Assessing and updating the reliability of concrete bridges subjected to spatial deterioration - principles and software implementation N2 - Inspection and maintenance of concrete bridges is a major cost factor in transportation infrastructure, and there is significant potential for using information gained during inspection to update predictive models of the performance and reliability of such structures. In this context, this paper presents an approach for assessing and updating the reliability of prestressed concrete bridges subjected to chloride-induced reinforcement corrosion. The system deterioration state is determined based on a Dynamic Bayesian Network (DBN) model that considers the spatial variability of the corrosion process. The overall system reliability is computed by means of a probabilistic structural model coupled with the deterioration model. Inspection data are included in the system reliability calculation through Bayesian updating on the basis of the DBN model. As proof of concept, a software prototype is developed to implement the method presented here. The software prototype is applied to a typical highway bridge and the influence of inspection information on the system deterioration state and the structural reliability is quantified taking into account the spatial correlation of the corrosion process. This work is a step towards developing a software tool that can be used by engineering practitioners to perform reliability assessments of ageing concrete bridges and update their reliability with inspection and monitoring data. KW - Structural reliability KW - Dynamic Bayesian Networks KW - Spatial deterioration KW - Inspection KW - Monitoring general KW - Analysis and design methods KW - Reinforcement KW - Corrosion KW - Prestressed concrete PY - 2015 DO - https://doi.org/10.1002/suco.201500014 SN - 1464-4177 VL - 16 IS - 3 SP - 356 EP - 365 PB - Ernst & Sohn CY - Berlin AN - OPUS4-34336 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Herbst, Tristan A1 - Schneider, S. A1 - Prediger, L. A1 - Flassenberg, G. ED - Hájek, P. ED - Tywoniak, J. ED - Lupísek, A. ED - Sojková, K. T1 - Sustainability analysis for the recycling of masonry T2 - CESB 13 - 3rd International conference Central Europe towards sustainable building - Sustainable building and refurbishment for next generations CY - Prague, Czech Republic DA - 2013-06-26 KW - Masonry KW - Recycling KW - Sustainability KW - LCA KW - LCC KW - LCWE PY - 2013 SN - 978-80-247-5015-6 SN - 978-80-247-5017-0 SP - 345 EP - 348 AN - OPUS4-30407 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Herbst, Tristan A1 - Schneider, S. A1 - Brandstetter, C.P. A1 - Flassenberg, G. T1 - Stoffkreislauf im Mauerwerksbau - Nachhaltigkeitsanalyse für das Mauerwerksrecycling N2 - In Deutschland fallen nach Abschätzungen von jährlich bis zu 10 Millionen Tonnen Mauerwerkbruch an. Im Sinne einer nachhaltigen Kreislaufwirtschaft sind diese Restmassen zukünftig einem möglichst hochwertigen und individuell angepassten Recycling zuzuführen. Vor diesem Hintergrund wurde im IGF-Forschungsvorhaben 17022 N der Forschungsvereinigung Porenbetonindustrie e.V. die Nachhaltigkeit verschiedener Recyclingoptionen für Mauerwerkbruch ganzheitlich untersucht. Unter Einbeziehung der Hauptmauersteinarten Ziegel, Kalksandstein, Porenbeton und Leichtbeton wurden dabei material- und verfahrenstechnische sowie ökologische, ökonomische und soziale Aspekte berücksichtigt. Als Ergebnis wurde ein EDV-gestütztes Analysetool (Web-Tool) zur Nachhaltigkeitsbewertung für das Mauerwerksrecycling entwickelt. Mit dem Web-Tool sollen Anwender (Planer, Architekten, Recycler) die Möglichkeit erhalten, für verschiedene Untersuchungsfälle unter Einbeziehung regional und sachlich bedingter Parameter objektspezifische Aussagen zur Nachhaltigkeit treffen zu können. Auf diesem Wege können im Sinne der aktuellen gesetzlichen Vorgaben nachhaltige Stoffkreisläufe im Mauerwerksbau geschlossen und somit letztlich die Deponierung von Mauerwerksrestmassen reduziert oder gar vermieden werden. T2 - R'13 - Fachtagung Recycling CY - Weimar, Germany DA - 19.09.2013 PY - 2013 SP - 1 EP - 6 PB - Bauhaus-Univ. CY - Weimar AN - OPUS4-30408 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schmidt, Stephan A1 - Hanelt, S. A1 - Canitz, C. A1 - Hoffmann, Holger A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Entwicklung monoklonaler Antikörper gegen Diclofenac N2 - Diclofenac (DCF) wird als nicht-steroidales, antientzündliches Arzneimittel gegen Fieber, Entzündungen, rheumatische Erkrankungen und Schmerzen eingesetzt. 70 % werden nach Einnahme wieder ausgeschieden. Aufgrund unvollständiger Elimination in den Kläranlagen lassen sich Rückstände im Trinkwasser und im Oberflächengewässer nachweisen. Diclofenac wird in der Umweltanalytik v.a. mittels GC-MS oder LCMS nachgewiesen. Bioanalytische Methoden machen sich die spezifische Antigen-Antikörper-Wechselwirkung zunutze, so z.B. Immunoassays. Die hierfür erforderlichen Antikörper werden durch Immunisierung mit einem Konjugat aus dem Analyten und einem Trägerprotein gewonnen. Die Kopplung kann direkt über die Carboxylfunktion von Diclofenac erfolgen. Um Abschirmungseffekte zu vermeiden, kann zwischen Protein und Analy1 ein Spacer sinnvoll sein. PY - 2015 SN - 1618-3258 SN - 1617-5301 IS - 4 SP - 107 EP - 109 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-35241 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Schneider, Ronald A1 - Fischer, J. A1 - Straub, D. A1 - Thöns, S. A1 - Bügler, M. A1 - Borrmann, A. T1 - Intelligente Bauwerke - Prototyp zur Ermittlung der Schadens- und Zustandsentwicklung für Elemente des Brückenmodells N2 - Dieser Bericht beschreibt ein Systemmodell für eine integrale Ermittlung und Prognose der Schadens- und Zustandsentwicklung der Elemente eines Brückensystems unter Berücksichtigung von Ergebnissen aus Inspektionen und Überwachung. Das Systemmodell wurde anhand eines ausgesuchten Spannbetonüberbaus in einzelliger Kastenbauweise entwickelt. Es besteht aus zwei integralen Teilmodellen: ein Modell zur Beschreibung des Systemschädigungszustandes und ein Modell zur Beschreibung der Standsicherheit. Für die Modellierung des stochastischen Systemschädigungszustandes eines Brückensystems werden dynamische Bayes'sche Netze (DBN) vorgeschlagen. Dieser Ansatz ermöglicht es, alle relevanten Schädigungsprozesse und deren stochastische Abhängigkeiten zu berücksichtigen. Ein wesentlicher Vorteil dieses Ansatzes ist es, dass DBN ideal dafür geeignet sind, Bayes'sche Aktualisierungen auf Grundlage von Informationen aus Inspektionen und Überwachungsmaßnahme auf eine effiziente und robuste Art und Weise durchzuführen. Der DBN-Ansatz ist deshalb für die Entwicklung von Software für das Erhaltungsmanagement von alternden Brückenbauwerken, die vom Benutzer keine vertieften Kenntnisse der Zuverlässigkeitstheorie verlangt, ideal geeignet. Für die Modellierung der Standsicherheit eines alternden Kastenträgers wird vereinfachend Biegeversagen des globalen Längssystems betrachtet. Zur Berechnung der maximalen Traglast eines Kastenträgers infolge des Systemschädigungszustandes wird ein plastisch-plastisches Verfahren eingesetzt, wobei die Beanspruchungen mittels der Fließgelenktheorie unter Ausnutzung der plastischen Beanspruchbarkeit der Querschnitte des Kastenträgers ermittelt werden. Ein Kastenträger versagt, wenn sich durch die Ausbildung einer ausreichend großen Anzahl von Fließgelenken eine kinematische Kette ausbildet. Dieser Modellierungsansatz berücksichtigt Redundanzen, die sich aus der plastischen Beanspruchbarkeit der Querschnitte und der statischen Unbestimmtheit eines Kastenträgers ergeben. Zum Nachweis der praktischen Einsetzbarkeit des entwickelten Systemmodells wurde ein Software-Prototyp entwickelt, der eine intuitiv benutzbare graphische Benutzeroberfläche (Front-End) mit einem Berechnungskern (Back-End) koppelt. Die aktuelle Version des Software-Prototyps implementiert ein Modell der chloridinduzierten Bewehrungskorrosion und ein Tragwerksmodell, welches das Verfahrens der stetigen Laststeigerung zur Bestimmung der maximalen Traglast des Kastenträgers auf der Grundlage eines Finite-Elemente-Modells umsetzt. Zur Durchführung von Bayes'schen Aktualisierungen des Systemschädigungszustandes auf der Grundlage des DBN-Modells implementiert der Prototyp den Likelihood-Weighting-Algorithmus. Die entwickelte Architektur des Prototyps ermöglicht eine Erweiterung der Software um weitere Schädigungsprozesse. Der entwickelte Software-Prototyp ermöglicht Benutzern ohne vertiefte Kenntnisse der Zuverlässigkeitstheorie eine Berechnung des Einflusses von Bauwerksinformationen auf den Systemschädigungszustand und die Tragsicherheit eines Kastenträgers. Auf dieser Grundlage können effiziente Inspektions- und Überwachungsmaßnahmen identifiziert und das Erhaltungsmanagement optimiert werden. KW - Bridge KW - Condition survey KW - Damage KW - Deterioration KW - Development KW - Digital model KW - Durability KW - Engineering structure KW - Expert system KW - Forecast KW - Germany KW - Propability KW - Prototype KW - Reinforced concrete KW - Research report KW - Software KW - Stability KW - Stochastic process PY - 2015 UR - http://bast.opus.hbz-nrw.de/volltexte/2015/1615/ SN - 978-3-95606-190-5 SN - 0943-9293 VL - 117 SP - 1 EP - 74 PB - Carl Schünemann Verlag GmbH CY - Bergisch Gladbach AN - OPUS4-35193 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Raysyan, Anna A1 - Galvidis, I. A. A1 - Schneider, Rudolf A1 - Eremin, S. A. A1 - Burkin, M. A. T1 - Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milk N2 - A lateral flow immunoassay (LFIA) using latex particles labeled with antibody to BSAclarithromycin (CLA) was developed for the rapid simultaneous group determination of six macrolide antibiotics. Optimization of antigen spotting on the membrane and latex probe loading allowed improving visual detectability (vLOD) 100 times, which was 1, 1, 10, 10, 50, and 1000 ng/mL for CLA, roxithromycin, erythromycin, dirithromycin, azithromycin, and oleandomycin in buffer, respectively. The calculated limits of instrumental detection (cLOD) were respectively 0.12, 0.15, 1.4, 2.1, 2.4, and 3.3 ng/mL. To avoid a strong influence of breast milk of a very diverse and variable composition, a sample pretreatment is proposed. The six macrolides mentioned can be visually detected in breast milk after 20 minutes pretreatment at concentrations of 10-1000 ng / ml or instrumentally with cLOD of 4.0, 2.5, 30, 42, 42 and 180 ng/mL. The recovery rate from the spiked samples carried out using a strip scanner device ranged from 71% to 110%, and precision expressed as relative standard deviation was between 3-14%. The described rapid on-site diagnostic assay format can be useful for monitoring the content of antibiotics in breast milk during macrolide treatment to ensure safe breastfeeding of infants. KW - Clarithromycin KW - Roxithromycin KW - Erythromycin KW - Dirithromycin KW - Azithromycin KW - Oleandomycin KW - Macrolide antibiotics KW - Makrolide KW - Antibiotika PY - 2020 DO - https://doi.org/10.1016/j.jpba.2020.113450 SN - 0731-7085 VL - 189 IS - 113450 SP - 1 EP - 9 PB - Elsevier CY - Amsterdam, NL AN - OPUS4-51343 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Raysyan, Anna A1 - Moerer, R. A1 - Coesfeld, Bianca A1 - Eremin, S. A1 - Schneider, Rudolf T1 - Fluorescence polarization immunoassay for the determination of diclofenac in wastewater N2 - Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was included in the European watch list of substances that requires its environmental monitoring in the member states. DCF may harmfully influence the ecosystem already at concentrations ≤ 1 μg L−1. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method which does not require the immobilization of reagents. FPIA can be performed in one phase within 20–30 min, making it possible to analyse wastewater without any complicated pre-treatment. In this study, new tracermolecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 μg L−1 with a working range up to 870 μg L−1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods. KW - Abwasser KW - Umweltschadstoffe KW - Antikörper KW - Antibody PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-518398 DO - https://doi.org/10.1007/s00216-020-03058-w SN - 1618-2642 VL - 413 IS - 4 SP - 999 EP - 107 PB - Springer CY - Heidelberg AN - OPUS4-51839 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Scala-Benuzzi, M. L. A1 - Soler-Illia, G. J. A. A. A1 - Rabia, J. A1 - Battaglini, F. A1 - Schneider, Rudolf A1 - Pereira, S. V. A1 - Messina, G. A. T1 - Immunosensor based on porous gold and reduced graphene platform for the determination of EE2 by electrochemical impedance spectroscopy N2 - In this work, we report an electrochemical immunosensor to detect ethinylestradiol in water samples, using electrochemical impedance spectroscopy (EIS) as a detection technique. For the development of this immunosensor, the direct modification of the working electrode of a screen-printed carbon electrode was carried out. First, to reduce the resistance of the electrode, electroreduced graphene was incorporated on the surface. Second, a porous gold structure was electrodeposited on reduced graphene by electrodeposition and the dynamic hydrogen bubble template assisted method. Thus, a marked increase in surface area was obtained for anti-EE2 antibodies immobilization. Subsequently, the specific anti-EE2 antibodies were covalently immobilized using α-lipoic acid for attaching them to the gold surface. The electrode modified with the antibodies was incubated for 30 min in the samples containing EE2, producing the specific Antigen antibody binding. As the charge transfer resistance of a redox probe in the electrode surface is governed by the surface blocking effects, the charge transfer resistance was related to the amount of EE2 captured to realize a quantitative determination. For this, the EIS measurements were performed in a 4 mM [Fe(CN)6]4−/3− solution in 0.1 M KCl. The obtained Nyquist diagrams were adjusted using the Randles circuit as an equivalent circuit to obtain the corresponding resistances. The developed methodology showed good selectivity, precision, and sensitivity; although the LOD obtained was higher than those presented in other published articles, it turned out to be an alternative that allows the determination of ethinylestradiol using a simple disposable electrode. KW - Ethinylestradiol KW - Biosensor KW - Elektrochemisch KW - Impedanz PY - 2021 DO - https://doi.org/10.1016/j.jelechem.2021.115604 SN - 1572-6657 VL - 897 SP - 115604 EP - 115611 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-54048 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Florian, Camilo A1 - Fischer, Daniel A1 - Freiberg, K. A1 - Duwe, M. A1 - Sahre, Mario A1 - Schneider, S. A1 - Hertwig, Andreas A1 - Krüger, Jörg A1 - Rettenmayr, M. A1 - Beck, Uwe A1 - Undisz, A. A1 - Bonse, Jörn T1 - Single Femtosecond Laser-Pulse-Induced Superficial Amorphization and Re-Crystallization of Silicon N2 - Superficial amorphization and re-crystallization of silicon in <111> and <100> orientation after irradiation by femtosecond laser pulses (790 nm, 30 fs) are studied using optical imaging and transmission electron microscopy. Spectroscopic imaging ellipsometry (SIE) allows fast data acquisition at multiple wavelengths and provides experimental data for calculating nanometric amorphous layer thickness profiles with micrometric lateral resolution based on a thin-film layer model. For a radially Gaussian laser beam and at moderate peak fluences above the melting and below the ablation thresholds, laterally parabolic amorphous layer profiles with maximum thicknesses of several tens of nanometers were quantitatively attained. The accuracy of the calculations is verified experimentally by high-resolution transmission electron microscopy (HRTEM) and energy dispersive X-ray spectroscopy (STEM-EDX). Along with topographic information obtained by atomic force microscopy (AFM), a comprehensive picture of the superficial re-solidification of silicon after local melting by femtosecond laser pulses is drawn. KW - Femtosecond laser KW - Silicon KW - Amorphization KW - Crystallization KW - Spectroscopic imaging ellipsometry PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-523687 UR - https://www.mdpi.com/1996-1944/14/7/1651 DO - https://doi.org/10.3390/ma14071651 SN - 1996-1944 VL - 14 IS - 7 SP - 1651-1 EP - 1651-21 PB - MDPI AG CY - Basel, Switzerland AN - OPUS4-52368 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, J. A1 - Farris, L. A1 - Nolze, Gert A1 - Reinsch, Stefan A1 - Cios, G. A1 - Tokarski, T. A1 - Thompson, S. T1 - Microstructure evolution in Inconel 718 produced by powder bed fusion additive manufacturing N2 - Inconel 718 is a precipitation strengthened, nickel-based super alloy of interest for the Additive Manufacturing (AM) of low volume, complex parts to reduce production time and cost compared to conventional subtractive processes. The AM process involves repeated rapid melting, solidification and reheating, which exposes the material to non-equilibrium conditions that affect elemental segregation and the subsequent formation of solidification phases, either beneficial or detrimental. These variations are difficult to characterize due to the small length scale within the micron sized melt pool. To understand how the non-equilibrium conditions affect the initial solidification phases and their critical temperatures, a multi-length scale, multi modal approach has been taken to evaluate various methods for identifying the initial phases formed in the as-built Inconel 718 produced by laser-powder bed fusion (L-PBF) additive manufacturing (AM). Using a range of characterization tools from the bulk differential thermal analysis (DTA) and x-ray diffraction (XRD) to spatially resolved images using a variety of electron microscopy tools, a better understanding is obtained of how these minor phases can be properly identified regarding the amount and size, morphology and distribution. Using the most promising characterization techniques for investigation of the as-built specimens, those techniques were used to evaluate the specimens after various heat treatments. During the sequence of heat treatments, the initial as-built dendritic structures recrystallized into well-defined grains whose size was dependent on the temperature. Although the resulting strength was similar in all heat treated specimens, the elongation increased as the grain size was refined due to differences in the precipitated phase distribution and morphology. KW - Metal additive manufacturing KW - Inconel 718 KW - Heat treatment KW - Grain boundary precipitates KW - Laves phase PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-542758 DO - https://doi.org/10.3390/jmmp6010020 SN - 2504-4494 VL - 6 IS - 1 SP - 1 EP - 20 PB - MDPI CY - Basel AN - OPUS4-54275 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Rudolf A1 - He, Y. A1 - Zhu, R. A1 - Cai, Y. A1 - Zhang, Y. A1 - Zhang, Y. A1 - Pan, S. A1 - Zhang, Y. T1 - Transcriptomics and protein biomarkers reveal the detoxifying mechanisms of UV radiation for nebivolol toward zebrafish (Danio rerio) embryos/larvae N2 - Nebivolol (NEB), a β-blocker frequently used to treat cardiovascular diseases, has been widely detected in aquatic environments, and can be degraded under exposure to UV radiation, leading to the formation of certain transformation products (UV-TPs). Thus, the toxic effects of NEB and its UV-TPs on aquatic organisms are of great importance for aquatic ecosystems. In the present study, the degradation pathway of NEB under UV radiation was investigated. Subsequently, zebrafish embryos/larvae were used to assess the median lethal concentration (LC50) of NEB, and to clarify the sub-lethal effects of NEB and its UV-TPs for the first time. It was found that UV radiation could reduce the toxic effects of NEB on the early development of zebrafish. Transcriptomic analysis identified the top 20 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in zebrafish larvae exposed to NEB, most of which were associated with the antioxidant, nervous, and immune systems. The number of differentially expressed genes (DEGs) in the pathways were reduced after UV radiation. Furthermore, the analysis of protein biomarkers, including CAT and GST (antioxidant response), AChE and ACh (neurotoxicity), CRP and LYS (immune response), revealed that NEB exposure reduced the activity of these biomarkers, whereas UV radiation could alleviate the effects. The present study provides initial insights into the mechanisms underlying toxic effects of NEB and the detoxification effects of UV radiation on the early development of zebrafish. It highlights the necessity of considering the toxicity of UV-TPs when evaluating the toxicity of emerging pollutants in aquatic systems. KW - Biomarker KW - Pharmazeutika KW - Toxikologie KW - UV Bestrahlung KW - Zebrafisch KW - Transformationsprodukte PY - 2022 DO - https://doi.org/10.1016/j.aquatox.2022.106241 SN - 0166-445X VL - 249 SP - 1 EP - 10 PB - Elsevier CY - Amsterdam AN - OPUS4-55559 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gregório, Bruno J.R. A1 - Ramos, Inês I. A1 - Marques, Sara S. A1 - Barreiros, Luísa A1 - Magalhães, Luís M. A1 - Schneider, Rudolf A1 - Segundo, Marcela A. T1 - Microcarrier-based fluorescent yeast estrogen screen assay for fast determination of endocrine disrupting compounds N2 - The presence of endocrine-disrupting compounds (EDCs) in water poses a significant threat to human and animal health, as recognized by regulatory agencies throughout the world. The Yeast Estrogen Screen (YES) assay is an excellent method to evaluate the presence of these compounds in water due to its simplicity and capacity to assess the bioaccessible forms/fractions of these compounds. In the presence of a compound with estrogenic activity, Saccharomyces cerevisiae cells, containing a lacZ reporter gene encoding the enzyme β-galactosidase, are induced, the enzyme is synthesised, and released to the extracellular medium. In this work, a YES-based approach encompassing the use of a lacZ reporter gene modified strain of S. cerevisiae, microcarriers as solid support, and a fluorescent substrate, fluorescein di-β-D-galactopyranoside, is proposed, allowing for the assessment of EDCs’ presence after only 2 h of incubation. The proposed method provided an EC50 of 0.17 ± 0.03 nM and an LLOQ of 0.03 nM, expressed as 17β-estradiol. The assessment of different EDCs provided EC50 values between 0.16 and 1.2 × 103 nM. After application to wastewaters, similar results were obtained for EDCs screening, much faster, compared to the conventional 45 h spectrophotometric procedure using a commercial kit, showing potential for onsite high-throughput screening of environmental contamination. KW - Biosensoren KW - YES assay KW - Endokrine Disruptoren PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-602315 DO - https://doi.org/10.1016/j.talanta.2024.125665 VL - 271 SP - 1 EP - 7 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-60231 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tamschick, S. A1 - Rozenblut-Kościsty, B. A1 - Ogielska, M. A1 - Lehmann, Andreas A1 - Lymberakis, P. A1 - Hoffmann, F. A1 - Lutz, I. A1 - Schneider, Rudolf A1 - Kloas, W. A1 - Stöck, M. T1 - Impaired gonadal and somatic development corroborate vulnerability differences to the synthetic estrogen ethinylestradiol among deeply diverged anuran lineages N2 - Amphibians are undergoing a global decline. One poorly investigated reason could be the pollution of aquatic habitats by endocrine disrupting compounds (EDCs). We tested the susceptibility to the synthetically stabilized estrogen 17α-ethinylestradiol (EE2) in three deeply diverged anuran species, differing in sex determination systems, types of gonadogenesis and larval ecologies. To understand whether data from the amphibian model Xenopus laevis (Pipidae) are analogous and applicable to only distantly related non-model amphibians, tadpoles of X. laevis, Hyla arborea (Hylidae) and Bufo viridis (Bufonidae) were simultaneously exposed to 50, 500 and 5000 ng/L EE2 from hatching until completion of metamorphosis, using a flow-through-system under identical experimental conditions. Comparing molecularly established genetic with histologically assessed phenotypic sex in all species, we have recently shown that EE2 provoked numerous genetic-male-to-phenotypic-female sex reversals and mixed sex individuals, confirming overall its expected feminizing effect. In the present study, we focus on the influence of EE2 on gonadal and somatic development. Anatomy and histology revealed several species-specific effects. In both non-model species, H. arborea and B. viridis, high numbers of anatomically impaired gonads were observed. In H. arborea, exposed to 5000 ng/L EE2, numerous underdeveloped gonads were detected. Whereas EE2 did not alter snout-to-vent length and body weight of X. laevis metamorphs, H. arborea showed a treatment-dependent decrease, while B. viridis exhibited an increase in body weight and snout-to-vent length. Apart from a concentration-dependent occurrence of yellowish skin color in several H. arborea, no organ-specific effects were detected. Since EE2 ubiquitously occurs in many aquatic ecosystems and affects sexual and somatic development, among EDCs, it may indeed contribute to amphibian decline. The inter-species variation in developmental EE2-effects corroborates species-specific vulnerability differences towards EDCs between deeply diverged amphibian groups KW - Endocrine disruption KW - Ethinylestradiol KW - Mass spectrometry PY - 2016 DO - https://doi.org/10.1016/j.aquatox.2016.07.001 SN - 0166-445X SN - 1879-1514 VL - 177 SP - 503 EP - 514 PB - Elsevier AN - OPUS4-37633 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Rudolf A1 - Carvalho, A. M. A1 - Manicardi, A. A1 - Montes, C. V. A1 - Gunnoo, S. B. A1 - Madder, A. T1 - Decoration of trastuzumab with short oligonucleotides: synthesis and detailed characterization N2 - Trastuzumab (Herceptin) is an FDA-approved therapeutic antibody currently employed in the treatment of metastatic stages of breast cancer. Herein, we propose a simple, fast and cost-effective methodology to conjugate trastuzumab with 22-mer 5' thiol-modified oligonucleotides using a bifunctional crosslinker. The conjugates were successfully characterized by MALDI-ToF MS and SDS-PAGE, obviating the need for enzymatic digestion and difficult chromatographic separations. Furthermore, ELISA was performed to ensure that trastuzumab activity is not affected by oligonucleotide conjugation. KW - MALDI-ToF KW - ELISA PY - 2017 DO - https://doi.org/10.1039/c7ob02216f SN - 1477-0539 VL - 15 IS - 42 SP - 8923 EP - 8928 AN - OPUS4-43328 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schneider, Rudolf A1 - Korsak, B. A1 - Almeida, G. M. A1 - Rocha, S. A1 - Pereira, C. A1 - Mendes, N. A1 - Osorio, H. A1 - Pereira, P. M. A1 - Rodrigues, J. M. M. A1 - Sarmento, B. A1 - Tome, J. P. C. A1 - Oliveira, C. T1 - Porphyrin modified trastuzumab improves efficacy of HER2 targeted photodynamic therapy of gastric cancer N2 - Gastric cancer (GC) is the 3rd deadliest cancer worldwide, due to limited treatment options and late diagnosis. Human epidermal growth factor receptor-2 (HER2) is overexpressed in similar to 20% of GC cases and anti-HER2 antibody trastuzumab in combination with conventional chemotherapy, is recognized as standard therapy for HER2-positive metastatic GC. This strategy improves GC patients' survival by 2-3 months, however its optimal results in breast cancer indicate that GC survival may be improved. A new photoimmunoconjugate was developed by conjugating a porphyrin with trastuzumab (Trast: Porph) for targeted photodynamic therapy in HER2-positive GC. Using mass spectrometry analysis, the lysine residues in the trastuzumab structure most prone for porphyrin conjugation were mapped. The in vitro data demonstrates that Trast: Porph specifically binds to HER2-positive cells, accumulates intracellularly, co-localizes with lysosomal marker LAMP1, and induces massive HER2-positive cell death upon cellular irradiation. The high selectivity and cytotoxicity of Trast: Porph based photoimmunotherapy is confirmed in vivo in comparison with trastuzumab alone, using nude mice xenografted with a HER2-positive GC cell line. In the setting of human disease, these data suggest that repetitive cycles of Trast: Porph photoimmunotherapy may be used as an improved treatment strategy in HER2-positive GC patients. KW - Photoimmunotherapy KW - Photoimmunoconjugate KW - Gastric cancer PY - 2017 DO - https://doi.org/10.1002/ijc.30844 SN - 0020-7136 VL - 141 IS - 7 SP - 1478 EP - 1489 PB - International Journal of Cancer AN - OPUS4-43315 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hakim, I. A1 - Schumacher, David A1 - Sundar, V. A1 - Donaldson, S. A1 - Creuz, A. A1 - Schneider, R. A1 - Keller, J. A1 - Browning, C. A1 - May, D. A1 - Abo Ras, M. A1 - Meyendorf, N. ED - Chimenti, D. E. ED - Bond, L. J. T1 - Volume imaging NDE and serial sectioning of carbon fiber composites N2 - A composite material is a combination of two or more materials with very different mechanical, thermal and electrical properties. The various forms of composite materials, due to their high material properties, are widely used as structural materials in the aviation, space, marine, automobile, and sports industries. However, some defects like voids, delamination, or inhomogeneous fiber distribution that form during the fabricating processes of composites can seriously affect the mechanical properties of the composite material. In this study, several imaging NDE techniques such as: thermography, high frequency eddy current, ultrasonic, x-ray radiography, x-ray laminography, and high resolution x-ray CT were conducted to characterize the microstructure of carbon fiber composites. Then, a 3D analysis was implemented by the destructive technique of serial sectioning for the same sample tested by the NDE methods. To better analyze the results of this work and extract a clear volume image for all features and defects contained in the composite material, an intensive comparison was conducted among hundreds of 3D-NDE and multi serial sections’ scan images showing the microstructure variation. T2 - 44TH ANNUAL REVIEW OF PROGRESS IN QUANTITATIVE NONDESTRUCTIVE EVALUATION CY - Provo, Utah, USA DA - 16.07.2017 KW - Carbon fiber reinforced polymers KW - Non-destructive testing KW - Thermography KW - Eddy current KW - Ultrasound KW - X-ray radiography KW - X-ray laminography KW - X-ray computed tomography KW - Serial sectioning PY - 2018 DO - https://doi.org/10.1063/1.5031590 VL - 1949 SP - 120003-1 EP - 120003-10 PB - AIP Publishing AN - OPUS4-45173 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sin, DWM. A1 - Wong, YC. A1 - Lehmann, Andreas A1 - Schneider, Rudolf A1 - Kakoulides, E. A1 - Lin, TT. A1 - Liu, QD. A1 - Cabillic, J. A1 - Lardy-fontan, S. A1 - Nammoonnoy, J. T1 - CCQM-K126: low polarity organic in water: carbamazepine in surface water N2 - This study aimed to assess the measurement capabilities of participating National Metrology Institutes/ Designated Institutes (NMIs/DIs) and expert laboratories in determining of low-polarity organics in surface water. This comparison was organized by Government Laboratory, Hong Kong (GLHK). At the CCQM Organic Analysis Working Group (OAWG) Meeting held in November 2012 in Hong Kong, GLHK initially proposed a CCQM key comparison and a parallel pilot study programme on pharmaceuticals in surface water. Further discussion at the CCQM Meeting held in April 2014 in Paris, the OAWG approved a CCQM Track C comparison (CCQM-K126) on low polarity pharmaceuticals in surface water. In the meeting, the programme was supported by more than six National Metrology Institutes/ Designated Institutes (NMIs/ DIs). CCQM-K126 officially commenced in July 2014 and had registration from nine NMIs/DIs. Participants were provided two bottles (40 mL each) of surface water and were requested to determine the mass fraction of spiked carbamazepinein in surface water. The coordinator received nine sets of results from eight NMIs/DIs in February 2015. Apart from one using an immunoassay technique, all participants applied isotope dilution liquid chromatography-tandem mass spectrometry (ID-LCMS/MS) technique as their determination technique. KW - CCQM-K126 KW - ELISA KW - Mass spectrometry PY - 2017 DO - https://doi.org/10.1088/0026-1394/54/1A/08030 SN - 0026-1394 SN - 1681-7575 VL - 54 IS - Suppl. S SP - Article 08030, 1 EP - 56 PB - IOP Publ. CY - Bristol AN - OPUS4-44831 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Rübner, Katrin A1 - Schneider, S. A1 - Beck, T. A1 - Flassenberg, G. A1 - Herbst, Tristan T1 - Stoffkreislauf im Mauerwerksbau - Nachhaltigkeitsanalyse für das Mauerwerksrecycling / Schlussbericht zum IGF-Vorhaben 17022 N N2 - In Deutschland fallen mittelfristig erhebliche Mengen von Mauerwerkbruch an. Aus dem jährlichen Bauschuttanfall und den Produktionszahlen von Wandbausteinen lassen sich die Mengen an Mauerwerksrestmassen ableiten, die als Rückbaumaterial bei Abriss-, Umbau und Sanierungsarbeiten in Deutschland anfallen werden. Hiernach ist pro Jahr mit bis zu 10 Millionen Tonnen heute vornehmlich heterogen anfallendem Mauerwerkbruch zu rechnen [1a]. Im Sinne einer nachhaltigen Kreislaufwirtschaft sind diese Restmassen zukünftig einem möglichst hochwertigen Recycling zuzuführen. In diesem Spannungsfeld führt die deutsche Mauersteinindustrie das breit aufgestellte gemeinschaftliche Forschungsprojekt "SIM Stoffkreislauf im Mauerwerksbau" durch. In insgesamt acht mauersteinübergreifenden IGFForschungsvorhaben wurden und werden technisch orientierte Lösungsmöglichkeiten für das Recycling von Mauerwerk erarbeitet und Anwendungsbereiche für Mauerwerksrestmassen aufgezeigt. Eine grundsätzliche Entscheidung über die Eignung von Mauerwerksrestmassen für bestimmte Recyclingwege kann es letztlich nicht geben. Es wird immer eine Einzelfallentscheidung bleiben, die wesentlich durch komplexe technische sowie ökologische und ökonomische Randbedingungen geprägt wird. Vor diesem Hintergrund wurde im Rahmen des IGF-Forschungsvorhabens 17022 N der Forschungsvereinigung Porenbetonindustrie e.V., Berlin die Nachhaltigkeit verschiedener Recyclingoptionen für Mauerwerkbruch ganzheitlich untersucht und bewertet. Unter Einbeziehung der Hauptwandbausteinarten Ziegel, Kalksandstein, Porenbeton und Leichtbeton wurden dabei material- und verfahrenstechnische sowie ökologische, ökonomische und soziale Aspekte berücksichtigt. Auf Grundlage der gewonnenen Erkenntnisse wurde ein EDV-gestütztes Analysetool (Web-Tool) zur Nachhaltigkeitsbewertung für das Mauerwerksrecycling entwickelt. Mit dem Web-Tool sollen Anwender (Planer, Architekten, Recycler) die Möglichkeit erhalten, für verschiedene Untersuchungsfälle unter Einbeziehung regional und sachlich bedingter Parameter, wie Materialqualität, Stoffmengen, Logistik, Aufbereitungstechnik, Anwendungsbereiche, Kosten und Erlöse, objektspezifische Aussagen zur Nachhaltigkeit treffen zu können. Auf diesem Wege können im Sinne der aktuellen gesetzlichen Vorgaben nachhaltige Stoffkreisläufe im Mauerwerksbau geschlossen und somit letztlich die Deponierung von Mauerwerksrestmassen vermieden werden. Alle vier Wandbaustoffarten haben sich im Rahmen des Forschungsvorhabens als recyclingfähig und hinsichtlich der Nachhaltigkeit als gleichwertig erwiesen. Das Ziel des Vorhabens wurde damit erreicht. KW - Nachhaltigkeit KW - Mauerwerkbruch KW - Ganzheitlichkeit PY - 2013 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-288572 SP - 1 EP - 182 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-28857 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hille, Falk A1 - Herrmann, Ralf A1 - Schneider, Ronald A1 - Pitters, S. A1 - Wedel, F. A1 - Hindersmann, I. T1 - Developing a guideline for structural health monitoring of road bridges in Germany N2 - In recent years, Structural Health Monitoring (SHM) has become a useful and increasingly widely used tool for supporting lifetime extensions of existing bridges with known structural deficiencies or indications of potentially critical damages or damage processes. At the same time, methods and tools are emerging, which enable monitoring-informed predictive maintenance of new and existing bridges based on digital twins. The monitoring process – starting from the definition of monitoring actions and ending with decisions based on monitoring outcomes – is complex and requires expertise in structural engineering, operation and maintenance of bridges, metrology, and data analytics. To support German road authorities, engineering consultancies, building contractors and other stakeholders of the bridge management, the Federal Highway Research Institute (BASt) has initiated the development of a new guideline for applying SHM as part of the management of road bridges. The guideline will present various use cases and for each identified use case, it will propose a proven monitoring scheme. In addition, the guideline will provide guidance on assessing the benefits of SHM as well as a common approach to managing monitoring data as a systematic basis for integrating monitoring data in the bridge management. This contribution discusses the motivation, objectives, and scope of the guideline, describes its use case centric structure and outlines the proposed data management. T2 - IABMAS 2024 CY - Kopenhagen, Danmark DA - 24.06.2024 KW - Guideline KW - Structural health monitoring KW - Road bridges KW - Infrastructure PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-613947 SN - 978-1-032-77040-6 DO - https://doi.org/10.1201/9781003483755-236 SP - 2009 EP - 2017 PB - CRC Press AN - OPUS4-61394 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Wedel, F. A1 - Pitters, S. A1 - Herrmann, Ralf A1 - Schneider, Ronald A1 - Hille, Falk A1 - Hindersmann, I. T1 - Guideline for the strategic application of monitoring of road bridges in Germany N2 - Engineering structures are an important part of our transport infrastructure. Their failure is associated with high safety risks and economic damage. Ensuring the availability of these constructions and guaranteeing their operational safety are thus important tasks. Currently, maintenance of engineering structures is a reactive process and therefore not yet a predictive process. Every 3 to 6 years, the structures are visually inspected, all damages are documented and a condition grade is assigned to the structure based on the detected damages. Continuous data-providing methods such as monitoring are used only very occasionally. With the help of monitoring, condition data about the structures are continuously collected, which can be used for a better assessment of the structures. On the one hand, this would increase safety of engineering structures, and on the other hand, it makes the shift towards predictive maintenance management based on real-time data and predictions, where the onset of damage is detected before it occurs, possible in the first place. Monitoring plays therefore an important role in the management of the infrastructure. It is a crucial a step towards the digitalisation of our infrastructure and existing processes. Although there are many use cases for monitoring that have already been successfully implemented, monitoring is not yet widely used by German road authorities due to several challenges that were also identified within the scope of the project. Therefore, a guide has been developed in which the current obstacles related to monitoring are identified and possibilities for integrating monitoring into existing processes are offered. To ensure optimal alignment with the needs of road authorities, both an online survey and a workshop have been conducted. The results are summarised in a brochure that is made available to authorities to improve the use of monitoring in Germany. This paper presents the guideline and the brochure. T2 - 10th European Workshop on Structural Health Monitoring (EWSHM 2024) CY - Potsdam, Germany DA - 10.06.2024 KW - Verkehrsinfrastrukturen KW - Engineering Structures KW - Bridges KW - Structural Health Monitoring KW - Guideline PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-612821 UR - https://www.ndt.net/search/docs.php3?id=29582 DO - https://doi.org/10.58286/29582 SN - 1435-4934 VL - 29 IS - 7 SP - 1 EP - 8 PB - NDT.net GmbH & Co. KG CY - Mayen AN - OPUS4-61282 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Herrmann, Ralf A1 - Hille, Falk A1 - Pitters, S. A1 - Ramasetti, Eshwar Kumar A1 - Schneider, Ronald A1 - Wedel, F. A1 - Hindersmann, I. ED - Rogge, Andreas ED - Meng, Birgit T1 - Föderiertes Datenmanagement von Monitoringdaten aus Structural Health Monitoring Anwendungen und daraus gewonnenen Trainingsdaten bei Spannbetonbrücken N2 - Beim Structural Health Monitoring entstehen eine Vielzahl von Daten, Metadaten und Modellen mit hohem Wert für die Beurteilung des Bauwerkszustands und der Prognose von Veränderungen. Kontinuierlich anwachsende Datenbestände müssen nachhaltig geteilt, genutzt und über die Bauwerkslebensdauer archiviert werden. Dabei stellen die Vielfalt der Messaufgaben, die Heterogenität der Daten, die dezentrale Erfassung und z. T. der Umfang eine Herausforderung für die beteiligten Akteure dar. Für den Datentransfer der Monitoringdaten zwischen der Straßenbauverwaltung und den Monitoringausführenden wird ein flexibles Abstimmungsverfahren vorgeschlagen und ein Lösungskonzept für ein föderiertes Datenmanagement skizziert. Eine weitere Herausforderung stellt die bauwerksübergreifende Zusammenstellung von Trainingsdaten für konkrete KI-Anwendungen dar. T2 - 11. Jahrestagung des DAfStb mit 63. Forschungskolloquium der BAM Green Intelligent Building CY - Berlin, Germany DA - 16.10.2024 KW - Structural Health Monitoring PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-612902 SN - 978-3-9818564-7-7 SP - 178 EP - 185 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-61290 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hille, Falk A1 - Herrmann, Ralf A1 - Pitters, S. A1 - Hindersmann, I. A1 - Schneider, Ronald A1 - Wedel, F. ED - Rogge, Andreas ED - Meng, Birgit T1 - Leitfaden für den strategischen Einsatz von Monitoring an Straßenbrücken N2 - Für die Anwendung von Structural Health Monitoring (SHM) im Rahmen des Managements von Straßenbrü-cken wurde zur Unterstützung von Straßenbauverwaltungen, Ingenieurbüros, Bauunternehmen und weiteren Akteuren die Erstellung eines Leitfadens initiiert. Dieser Leitfaden stellt typische Anwendungsfälle für Monito-ring vor und schlägt für diese bewährte Herangehensweisen vor. Darüber hinaus bietet der Leitfaden Empfeh-lungen für Ausschreibung und Vergabe von Monitoringleistungen, eine Anleitung zur Bewertung des wirt-schaftlichen Nutzens von SHM, Hinweise zur optimal einsetzbaren Messtechnik sowie einen Ansatz für das Datenmanagement zur Integration der Monitoringdaten in das Brückenmanagement. T2 - 11. Jahrestagung des DAfStb mit 63. Forschungskolloquium der BAM Green Intelligent Building CY - Berlin, Germany DA - 16.10.2024 KW - Leitfaden KW - Monitoring KW - Straßenbrücke PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-612931 SN - 978-3-9818564-7-7 SP - 186 EP - 191 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-61293 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -