TY - JOUR A1 - Müller, Waltraut A1 - Koynov, K. A1 - Pierrat, S. A1 - Thiermann, Raphael A1 - Fischer, K. A1 - Maskos, Michael T1 - pH-change protective PB-b-PEO polymersomes N2 - Poly(butadiene)-b-poly(ethylene oxide) vesicles were successfully loaded with hydrophilic dye Phloxine B. Dye addition during vesicle formation leads to Phloxine B encapsulated inside the water filled vesicle core as well as to freely diffusing dye molecules. The removal of uncapsulated substrate involves time consuming methods like dialysis or harsher methods like ultra filtration or selective precipitation, posing the risk of irreversible sample manipulation. Here used Phloxine B as pH sensitive fluorescence indicator allows the characterization of hydrophilic loading without separation procedure by adjusting the pH value. Additionally membrane blocking efficiency can be studied by time dependent fluorescence measurements. Cryogenic TEM studies showed that the self-assembled structure remained unchanged when the hydrophilic dye was incorporated within the vesicles. Fluorescence microscopy imaging proved the encapsulation of the hydrophilic dye inside the core volume. The combination of fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS) measurements as ensemble methods confirmed those results additionally. KW - Diblock copolymers KW - Self assembly KW - Vesicle loading PY - 2011 DO - https://doi.org/10.1016/j.polymer.2011.01.028 SN - 0032-3861 SN - 1873-2291 VL - 52 IS - 5 SP - 1263 EP - 1267 PB - Elsevier Ltd. AN - OPUS4-23520 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -