TY - JOUR A1 - Mix, Renate A1 - Hoffmann, Katrin A1 - Buschmann, H.-J. A1 - Friedrich, Jörg Florian A1 - Resch-Genger, Ute T1 - Anbindung von Fluoreszenzfarbstoffen an plasmachemisch funktionalisierte und Cucurbituril-modifizierte Oberflächen N2 - Plasmapolymerisierte Allylaminschichten (50 nm) wurden auf Polypropylenfolien abgeschieden. Die auf diese Weise generierte NH2-funktionalisierte Polymeroberfläche wurde mit Xanthen-Fluorophoren chemisch umgesetzt. Da die Farbstoff-gekoppelten Folien nur geringe Fluoreszenz aufwiesen, wurden zwei Wege zur möglichen Entkopplung der Farbstoffe von der Oberfläche und damit zur Erhöhung der Fluoreszenzintensität getestet. Als erstes wurde ein Spacer durch die Umsetzung der primären Aminogruppen mit Glutaraldehyd und der nachfolgenden Kettenverlängerung mit Diaminohexan eingeführt. Diese nachfolgende Reaktion ergab eine spacergebundene NH2-Oberfläche, die analog der Allylaminschicht mit den Farbstoffen umgesetzt wurde. Als weiterer Weg wurde der Zusatz einer geeigneten Käfigverbindung, des Cucurbit[6]urils, getestet. Die spacergebundenen und zusätzlich mit Cucurbituril modifizierten aminofunktionellen Oberflächen zeigten nach der Farbstoffankopplung eine verbesserte Fluoreszenzintensität. KW - Fluoreszenz KW - Spacer KW - Cucurbituril PY - 2007 DO - https://doi.org/10.1002/vipr.200700329 SN - 0947-076X SN - 1522-2454 VL - 19 IS - 5 SP - 31 EP - 37 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-16000 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hennrich, Gunther A1 - Sonnenschein, Helmut A1 - Resch-Genger, Ute T1 - Fluorescent anion receptors with iminoylthiourea binding sites-selective hydrogen bond mediated recognition of CO3 2-, HCO3- and HPO4 2- N2 - A number of structurally simple, fluorescent sensor molecules based on the iminoylthiourea/1,2,4-thiadiazole unit are presented, which display extraordinarily strong fluorescence enhancement selectively upon complexation of HCO3-, CO32- and HPO42-. PY - 2001 DO - https://doi.org/10.1016/S0040-4039(01)00324-0 SN - 0040-4039 SN - 1873-3581 VL - 42 IS - 15 SP - 2805 EP - 2808 PB - Elsevier CY - Amsterdam AN - OPUS4-2110 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Getting it right with fluorescence: Fluorescence standards at BAM T2 - BERM 10, Tenth International Symposium on Biological and Environmental Reference Materials CY - Charleston, SC, USA DA - 2006-04-30 PY - 2006 AN - OPUS4-12594 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rurack, Knut A1 - Unger, Brita A1 - Müller, Ralf A1 - Jancke, Harald A1 - Resch-Genger, Ute T1 - Die Strukturentwicklung von SiO2 Solen und Gelen im Vergleich mikroskopischer und makroskopischer Untersuchungsmethoden T2 - 77. Glastechnische Tagung CY - Leipzig, Deutschland DA - 2003-05-26 PY - 2003 VL - 77 SP - 101 EP - 104 PB - DGG CY - Offenbach AN - OPUS4-3121 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Absolute fluorometry in the life and material sciences Characterization of NIR fluorophores, fluorescent particles and scattering and fluorescent systems T2 - Biosensor Symposium CY - Wildau, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-29636 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - A new detection method for the sensitive measurement of interactions on BioChips: Rolling circle amplification (RCA) T2 - DECHEMA Statusseminar Chiptechnologien CY - Frankfurt am Main, Germany DA - 2008-01-31 PY - 2008 AN - OPUS4-18550 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Zavoiura, Oleksandr A1 - Resch-Genger, Ute A1 - Seitz, Oliver T1 - Quantum dot-PNA conjugates for target-catalyzed RNA detection N2 - Detection of pathogenic nucleic acids remains one of the most reliable approaches for the diagnosis of a broad range of diseases. Current PCR-based methods require experienced personnel and cannot be easily used for point-of care diagnostics, making alternative strategies for the sensitive, reliable, and cost-efficient detection of pathogenic nucleic acids highly desirable. Here, we report an enzyme-free method for the fluorometric detection of RNA that relies on a target-induced fluorophore transfer onto a semiconductor quantum dot (QD), uses PNA probes as selective recognition elements and can be read out with simple and inexpensive equipment. For QD-PNA conjugates with optimized PNA content, limits of detection of dengue RNA in the range of 10 pM to 100 nM can be realized within 5 h in the presence of a high excess of noncomplementary RNA. KW - FRET KW - Fluorescence KW - DNA KW - Assay KW - Quantum dot KW - Nano KW - Particle KW - Synthesis KW - Ligation Assay PY - 2018 DO - https://doi.org/10.1021/acs.bioconjchem.8b00157 SN - 1043-1802 VL - 29 IS - 5 SP - 1690 EP - 1702 PB - ACS Publications AN - OPUS4-45120 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Simple and Versatile Methods for Quantifying Functional Groups, Ligands, and Biomolecules on Nanomaterials N2 - Many applications of nanomaterials in the life sciences require the controlled functionalization of these materials with ligands like polyethylene glycol (PEG) and/or biomolecules such as peptides, proteins, and DNA. This enables to tune their hydrophilicity and biocompatibility, minimize unspecific interactions, improve biofunctionalization efficiencies, and enhance blood circulation times. Moreover, it is the ultimate prerequisite for their use as reporters in assays or the design of targeted optial probes for bioimaging. At the core of these functionalization strategies are reliable and validated methods for surface group and ligand quantification that can be preferably performed with routine laboratory instrumentation, require only small amounts of substances, and are suitable for many different types of nanomaterials. We present here versatile and simple concepts for the quantification of common functional groups, ligands, and biomolecules on different types of organic and inorganic nano-materials, using different types of optical reporters and method validation with the aid of multimodal reporters, method comparisons, and mass balances. T2 - INNANOPART open day CY - London, UK DA - 24.04.2018 KW - Surface functionalization KW - Nanoparticles KW - Surface chemistry KW - Multimodal reporters PY - 2018 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-44849 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sonnenschein, Helmut A1 - Resch-Genger, Ute A1 - Hennrich, Gunther T1 - Schaltbare Fluoreszenzmarker zur Erkennung von Übergangs- und Schwermetallen KW - Fluoreszenz KW - Metallionennachweis KW - Redoxschalter KW - Molekülspektroskopie PY - 2001 SN - 0016-3538 VL - 2 SP - 2 EP - 3 PB - GIT-Verlag CY - Darmstadt AN - OPUS4-2112 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - State-of-the-Art in Fluorescence Measurements T2 - IUPAC Task Force Group Fluorescence CY - Lisbon, Portugal DA - 2005-09-04 PY - 2005 AN - OPUS4-10829 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Linck, Lena A1 - Resch-Genger, Ute T1 - Optimized Fluorescence Detection for RCA-Assays on Microarrays T2 - Kolloquium CY - Potsdam, Germany DA - 2010-08-25 PY - 2010 AN - OPUS4-22081 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Spectroscopic properties of upconversion nanoparticles N2 - Lanthanide-doped up-converting nanoparticles (UCNPs) are promising reporters for medical diagnostics and bioimaging. Current limitations present their relative low absorption cross sections and low fluorescence efficiencies, with the latter being affected by particle size, surface chemistry, and microenvironment, particularly water. Here, we present results from systematic studies of the excitation power density dependent upconversion luminescence spectra, intensities/intensity ratios of the individual emission bands, slope factors, and quantum yields of UCNPs of varying size, dopant concentration, and surface chemistry in different microenvironments as well as the up- and downconversion luminescence decay kinetics of the different emission bands. Moreover, first studies of the energy transfer from UCNPs to surface-bound organic dyes acting as fluorescence acceptors are shown. Based upon these measurements, fluorescence deactivation channels are identified and spectroscopic parameters for the screening of material performance are derived. T2 - Projekttreffen COST CY - Straßbourg, France DA - 09.01.2017 KW - Upconverting nanoparticles KW - Size KW - FRET KW - Fluorescence KW - Absolute fluorescence quantum yield KW - Fluorescence decay kinetics KW - Power density dependence PY - 2017 AN - OPUS4-39076 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Molecular and Particulate Labels and Probes for Optical Imaging T2 - Exzellenzakademie Medizintechnik: Krankheitsspezifische & molekulare Bildgebung CY - Aachen, Germany DA - 2010-04-26 PY - 2010 AN - OPUS4-21544 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -