TY - JOUR A1 - Beyer, S. A1 - Kimani, Martha Wamaitha A1 - Zhang, Y. A1 - Verhassel, A. A1 - Sternbæk, L. A1 - Wang, T. A1 - Persson, J. L. A1 - Härkönen, P. A1 - Johansson, E. A1 - Caraballo, R. A1 - Elofsson, M. A1 - Gawlitza, Kornelia A1 - Rurack, Knut A1 - Ohlsson, L. A1 - El-Schich, Z. A1 - Gjörloff Wingren, A. A1 - Stollenwerk, M. M. T1 - Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-coated Polystyrene Cores - Assessment of the Binding Behavior to Cancer Cells N2 - Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the alpha-2,3- and alpha-2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, alpha-2,3-SA) and Sambucus Nigra Lectin (SNA, alpha-2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells. KW - Cancer KW - Imprinting KW - Molecularly imprinted polymers KW - SA conjugates KW - Sialic acid PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-546625 DO - https://doi.org/110.3390/cancers14081875 SN - 2072-6694 VL - 14 IS - 8 PB - MDPI CY - Basel AN - OPUS4-54662 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Fernández, R. A1 - Bokuchava, G. A1 - Toda-Caraballo, I. A1 - Bruno, Giovanni A1 - Turchenko, V. A1 - Gorshkova, Y. A1 - González-Doncel, G. T1 - Analysis of the Combined Strengthening Effect of Solute Atoms and Precipitates on Creep of Aluminum Alloys N2 - The creep strengthening mechanisms in (age-hardenable) aluminum alloys are analyzed on the basis of a new microstructural study of powder samples, an analysis of a comprehensive revision of creep data from the literature, and a new modeling approach. A strategy based on the strength difference (SD) method to separate the contributions of solid solution atoms and precipitates to creep strengthening is proposed. The new methodology considers the combination of the two contributions avoiding the need of a threshold stress term in the creep equation. The contribution of both precipitates and solid solution is taken into account by means of the analysis of the lattice parameter variation with aging time. For this study, powders of two commercial AA2xxx alloys have been analyzed using diffraction methods. The experimental results are modeled using Lubarda's approach combined with the SD method. KW - Aluminium KW - Creep KW - Diffraction KW - Precipitation KW - Solute atoms PY - 2020 DO - https://doi.org/10.1002/adem.201901355 SN - 1438-1656 VL - 22 SP - 1901355 EP - 4 PB - Wiley-VCH Verlag AN - OPUS4-50424 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Fernández, R. A1 - Bokuchava, G. A1 - Toda-Caraballo, I. A1 - Bruno, Giovanni A1 - Turchenko, V. A1 - Gorshkova, Y. A1 - González-Doncel, G. T1 - Analysis of the Combined Strengthening Effect of Solute Atoms and Precipitates on Creep of Aluminum Alloys N2 - The creep strengthening mechanisms in (age-hardenable) aluminum alloys are analyzed on the basis of a new microstructural study of powder samples, an analysis of a comprehensive revision of creep data from the literature, and a new modeling approach. A strategy based on the strength difference (SD) method to separate the contributions of solid solution atoms and precipitates to creep strengthening is proposed. The new methodology considers the combination of the two contributions avoiding the need of a threshold stress term in the creep equation. The contribution of both precipitates and solid solution is taken into account by means of the analysis of the lattice parameter variation with aging time. For this study, powders of two commercial AA2xxx alloys have been analyzed using diffraction methods. The experimental results are modeled using Lubarda’s approach combined with the SD method. KW - Composites KW - Aluminum alloys KW - Creep models KW - Neutron Diffraction KW - Stress exponent PY - 2020 DO - https://doi.org/10.1002/adem.201901355 VL - 22 IS - 4 SP - 1901355 PB - WILEY-VCH Verlag AN - OPUS4-51055 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -