TY - JOUR A1 - Becker, Günther A1 - Noack, D. A1 - Schulz, H. T1 - Holzforschung PY - 1974 SN - 0018-3792 VL - 100 IS - 147 SP - 290, 294-296, 300-301 PB - DRW-Verl. Weinbrenner CY - Leinfelden-Echterdingen AN - OPUS4-8049 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Erhard, Anton A1 - Völzke, Holger A1 - Noack, Volker A1 - Weber, Wolfgang A1 - Wolff, Dietmar A1 - Rittscher, D. A1 - Schmidt, H. T1 - Long term interim storage facility for nuclear reactor compartments of submarines - German support for utilization of nuclear submarines in Russia T2 - PATRAM 2007, 15th International Symposium on the Packaging and Transportation of Radioactive Materials CY - Miami, Florida, USA DA - 2007-10-21 PY - 2007 SP - 155 PB - Institute of Nuclear Materials Management AN - OPUS4-16157 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Ferrero, Fabio A1 - Kluge, Martin A1 - Schmidtchen, Ulrich A1 - Pahl, Robert A1 - Noack, Jennifer A1 - Kolvitz, Kristian A1 - Runge, Thomas A1 - Holtappels, Kai A1 - Hensel, Christina A1 - Bönig, Jürgen A1 - Schulze, Uwe A1 - Askar, Enis A1 - Frank, M. A1 - Thornton, D. T1 - Experimentelle und numerische Untersuchungen des Verhaltens von Acetylenflaschen im Feuer T2 - 12. Kolloquium zu Fragen der chemischen und physikalischen Sicherheitstechnik CY - Berlin, Deutschland DA - 2010-06-15 KW - Acetylendruckgasbehälter KW - Unterfeuerung KW - Aufheizverhalten KW - CFD-Modellierung PY - 2010 SN - 978-3-9813550-1-7 SN - 0938-5533 N1 - Geburtsname von Kluge, Martin: Beckmann-Kluge, M. - Birth name of Kluge, Martin: Beckmann-Kluge, M. SP - 70 EP - 72 PB - BAM Bundesanstalt für Materialforschung und -prüfung CY - Berlin AN - OPUS4-21488 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schiebel, J. A1 - Noack, J. A1 - Rödiger, S. A1 - Kammel, A. A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Weise, Matthias A1 - Weiss, R. A1 - Böhm, A. A1 - Nitschke, J. A1 - Elimport, A. A1 - Roggenbuck, D. A1 - Schierack, P. T1 - Analysis of three-dimensional biofilms on different material surfaces N2 - Biofilms cause complications and high costs in both industry and medicine. Of particular interest are bacterial infections of prosthetic materials, which usually cannot be eliminated due to the high antibiotic resistance known for bacteria forming biofilms. The search for new materials and coatings with lower colonization potential and antibacterial activity is of great importance to reduce biofilm formation. However, there is no standardized procedure to examine the colonization characteristics of bacteria in the Biofilm state in situ. Here, we describe an automated epifluorescence microscopy system for the semi-quantitative analysis of three-dimensional (3D) biofilms on various surfaces. To analyze adherent bacteria, three materials (glass, steel and titanium) were incubated with bacteria in a flow chamber system. After fluorescence staining of the bacteria, automated image capturing, quantification of the bacteria, measurement of the colonized area and determination of the 3D biofilm height were carried out by using novel software. Furthermore, the materials were examined for their surface topography using white light scanning interferometry. Titanium compared to glass showed a significantly higher number of adherent bacteria. We argue that this was due to the higher microroughness of titanium. The colonized area was in accordance with the number of adherent bacteria and was also significantly larger on titanium coupons compared to glass. Maximum 3D biofilm height on glass coupons was significantly lower compared to the ones on steel and titanium. This novel method enables the standardized, automated investigation of the colonization with bacteria on different materials. This approach can considerably support the characterization of new material surfaces and their innovative coatings by analyzing the amount of attached Bacteria and thickness of biofilms in situ and eliminates the need of conventional cultivation. KW - Biofilm KW - Bacterial adhesion KW - Biofilm quantification KW - Automated analysis PY - 2020 DO - https://doi.org/10.1039/D0BM00455C SP - 1 EP - 11 PB - Royal Society of Chemistry AN - OPUS4-50815 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Erhard, Anton A1 - Völzke, Holger A1 - Noack, Volker A1 - Weber, Wolfgang A1 - Wolff, Dietmar A1 - Rittscher, D. A1 - Schmidt, H. T1 - Construction of long term interim storage facility for nuclear reactor compartments of submarines: German support for utilisation of nuclear submarines in Russia KW - Interim storage facility KW - Nuclear reactor compartments of submarines KW - Building of the storage facility KW - Transportation of heavy compartments PY - 2008 SN - 1746-5095 SN - 1746-5109 SN - 0957-476X VL - 19 IS - 2 SP - 117 EP - 121 PB - Ramtrans Publ. CY - Ashford AN - OPUS4-17708 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Noack-Schönmann, Steffi A1 - Bus, T. A1 - Banasiak, Ronald A1 - Knabe, Nicole A1 - Broughton, William J. A1 - Dulk-Ras, H.D. A1 - Hooykaas, P.J.J. A1 - Gorbushina, Anna T1 - Genetic transformation of Knufia petricola A95 - a model organism for biofilm-material interactions N2 - We established a protoplast-based system to transfer DNA to Knufia petricola strain A95, a melanised rock-inhabiting microcolonial fungus that is also a component of a model sub-aerial biofilm (SAB) system. To test whether the desiccation resistant, highly melanised cell walls would hinder protoplast formation, we treated a melanin-minus mutant of A95 as well as the type-strain with a variety of cell-degrading enzymes. Of the different enzymes tested, lysing enzymes from Trichoderma harzianum were most effective in producing protoplasts. This mixture was equally effective on the melanin-minus mutant and the type-strain. Protoplasts produced using lysing enzymes were mixed with polyethyleneglycol (PEG) and plasmid pCB1004 which contains the hygromycin B (HmB) phosphotransferase (hph) gene under the control of the Aspergillus nidulans trpC. Integration and expression of hph into the A95 genome conferred hygromycin resistance upon the transformants. Two weeks after plating out on selective agar containing HmB, the protoplasts developed cell-walls and formed colonies. Transformation frequencies were in the range 36 to 87 transformants per 10 µg of vector DNA and 106 protoplasts. Stability of transformation was confirmed by sub-culturing the putative transformants on selective agar containing HmB as well as by PCR-detection of the hph gene in the colonies. The hph gene was stably integrated as shown by five subsequent passages with and without selection pressure. KW - DNA transfer KW - Fungal cell-walls KW - Protoplasts KW - Hygromycin resistance KW - Black yeast KW - Sub-aerial biofilms KW - Stress-protective morphology KW - Ancestor of opportunistic pathogens & lichens PY - 2014 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-320840 UR - http://www.amb-express.com/content/4/1/80 DO - https://doi.org/10.1186/s13568-014-0080-5 SN - 2191-0855 VL - 4 SP - 80, 1 EP - 6 PB - Springer CY - Heidelberg AN - OPUS4-32084 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -