TY - GEN A1 - Breitenbach, Romy A1 - Gerrits, Ruben A1 - Dementyeva, Polina A1 - Knabe, Nicole A1 - Schumacher, Julia A1 - Feldmann, Ines A1 - Radnik, Jörg A1 - Ryo, M. A1 - Gorbushina, Anna T1 - Data for "The role of extracellular polymeric substances of fungal biofilms in mineral attachment and weathering" N2 - Data for the publication "The role of extracellular polymeric substances of fungal biofilms in mineral attachment and weathering" (https://doi.org/10.1038/s41529-022-00253-1). It includes: - The Summary of the EPS concentration, EPS sugar components and EPS linkages. - The Summary of the XPS analysis of freeze-dried biofilm samples of all strains. - The Summary of the pH, Mg, SI and Fe concentration, biomass and olivine dissolution rate for each time point of all dissolution experiments. KW - Biofilms PY - 2022 U6 - https://doi.org/10.26272/opus4-54901 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-54901 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Breitenbach, Romy A1 - Gerrits, Ruben A1 - Dementyeva, Polina A1 - Knabe, Nicole A1 - Schumacher, Julia A1 - Feldmann, Ines A1 - Radnik, Jörg A1 - Ryo, M. A1 - Gorbushina, Anna T1 - The role of extracellular polymeric substances of fungal biofilms in mineral attachment and weathering N2 - The roles extracellular polymeric substances (EPS) play in mineral attachment and weathering were studied using genetically modified biofilms of the rock-inhabiting fungus Knufia petricola strain A95. Mutants deficient in melanin and/or carotenoid synthesis were grown as air-exposed biofilms. Extracted EPS were quantified and characterised using a combination of analytical techniques. The absence of melanin affected the quantity and composition of the produced EPS: mutants no longer able to form melanin synthesised more EPS containing fewer pullulan-related glycosidic linkages. Moreover, the melanin-producing strains attached more strongly to the mineral olivine and dissolved it at a higher rate. We hypothesise that the pullulan-related linkages, with their known adhesion functionality, enable fungal attachment and weathering. The released phenolic intermediates of melanin synthesis in the Δsdh1 mutant might play a role similar to Fe-chelating siderophores, driving olivine dissolution even further. These data demonstrate the need for careful compositional and quantitative analyses of biofilm-created microenvironments. KW - Biofilms PY - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:b43-549025 SN - 2397-2106 VL - 6 SP - 1 EP - 11 PB - Springer Nature CY - London AN - OPUS4-54902 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Noack-Schönmann, Steffi A1 - Bus, T. A1 - Banasiak, Ronald A1 - Knabe, Nicole A1 - Broughton, William J. A1 - Dulk-Ras, H.D. A1 - Hooykaas, P.J.J. A1 - Gorbushina, Anna T1 - Genetic transformation of Knufia petricola A95 - a model organism for biofilm-material interactions N2 - We established a protoplast-based system to transfer DNA to Knufia petricola strain A95, a melanised rock-inhabiting microcolonial fungus that is also a component of a model sub-aerial biofilm (SAB) system. To test whether the desiccation resistant, highly melanised cell walls would hinder protoplast formation, we treated a melanin-minus mutant of A95 as well as the type-strain with a variety of cell-degrading enzymes. Of the different enzymes tested, lysing enzymes from Trichoderma harzianum were most effective in producing protoplasts. This mixture was equally effective on the melanin-minus mutant and the type-strain. Protoplasts produced using lysing enzymes were mixed with polyethyleneglycol (PEG) and plasmid pCB1004 which contains the hygromycin B (HmB) phosphotransferase (hph) gene under the control of the Aspergillus nidulans trpC. Integration and expression of hph into the A95 genome conferred hygromycin resistance upon the transformants. Two weeks after plating out on selective agar containing HmB, the protoplasts developed cell-walls and formed colonies. Transformation frequencies were in the range 36 to 87 transformants per 10 µg of vector DNA and 106 protoplasts. Stability of transformation was confirmed by sub-culturing the putative transformants on selective agar containing HmB as well as by PCR-detection of the hph gene in the colonies. The hph gene was stably integrated as shown by five subsequent passages with and without selection pressure. KW - DNA transfer KW - Fungal cell-walls KW - Protoplasts KW - Hygromycin resistance KW - Black yeast KW - Sub-aerial biofilms KW - Stress-protective morphology KW - Ancestor of opportunistic pathogens & lichens PY - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:b43-320840 UR - http://www.amb-express.com/content/4/1/80 SN - 2191-0855 VL - 4 SP - 80, 1 EP - 6 PB - Springer CY - Heidelberg AN - OPUS4-32084 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Nicole A1 - Breitenbach, Romy A1 - Dementyeva, Polina A1 - Heeger, F. A1 - Mazzoni, C. A1 - Gorbushina, Anna T1 - Differential gene expression and oxidative stress tolerance in the rock-inhabiting fungus Knufia petricola A95 N2 - Microcolonial fungi (MCF) are able to survive extreme environmental conditions. This study is focusing on responses to oxidative stress - using the rock fungus Knufia petricola strain A95. Comparative gene expression analyses of a wild type and a PKS mutant are used to identify genes which are especially regulated under oxidative stress conditions to help elucidate mechanisms of cell wall maturation and oxidative stress defence strategies. T2 - VAAM-Jahrestagung 2016 CY - Jena, Germany DA - 13.03.2016 KW - MCF KW - RNA-Seq KW - Oxidative stress PY - 2016 AN - OPUS4-37966 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Nicole A1 - Breitenbach, Romy A1 - Dementyeva, Polina A1 - Gorbushina, Anna T1 - Genetic manipulation of protective pigments in a rock-inhabiting model fungus Knufia petricola A95 N2 - Black ascomycetous microcolonial fungi are persistent inhabitants of rock surfaces, but are especially conspicuous in hostile environments like cold and hot deserts. The unique robustness of MFCs is supported by protective pigments, like melanin and carotenoids. We study the role of these pigments in the stress resistance of the model rock fungus Knufia petricola (Chaetothyriales) strain A95. T2 - 13th European Conference of Fungal Genetics CY - Paris, France DA - 03.04.2016 KW - MCF KW - EPS KW - Pigments PY - 2016 AN - OPUS4-37969 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Nicole A1 - Breitenbach, Romy A1 - Dementyeva, Polina A1 - Gorbushina, Anna T1 - Why some like it on the rocks: Recurring stresses select for organisms with manifold protective pigments N2 - Microcolonial fungi (MCF) are the most stress-resistant eukaryotes known to date. Black fungi are an interesting object to study mechanisms of stress resistance and to perform applied research to prevent material colonization and biodeterioration. Pigments, like melanin and carotenoids, have been proven to contribute to the unique robustness of MFCs. We study how these pigments ameliorate oxidative stress responses - one of the most significant environmental challenges encountered by MCF - using the model rock fungus Knufia petricola (Chaetothyriales) strain A95. T2 - VAAM-Jahrestagung 2016 CY - Jena, Germany DA - 13.03.2016 KW - MCF KW - Oxidative stress KW - Pigment PY - 2016 AN - OPUS4-37970 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Nicole A1 - Dementyeva, Polina A1 - Gerrits, Ruben A1 - Banasiak, Ronald A1 - Pokharel, R. A1 - Schüssler, J. A. A1 - von Blanckenburg, F. A1 - Gorbushina, Anna T1 - A new tool in material science - Targeted disruption of melanin synthesis in rock-inhabiting fungi N2 - The ability to survive almost absolute dehydration through air-drying is a remarkable feature of rock-inhabiting microcolonial fungi (MCF), which colonise rock surfaces in hot and cold deserts. Understanding of the underlying mechanisms which allow this group of fungi to conquer natural and man-made environments requires a set of modern biological techniques and approaches that are under development in our laboratory. We will present an overview of the targeted disruption of melanin biosynthesis genes in the rock-inhabiting Knufia petricola and give inside into the lines of research and the network of supporting laboratories that made this progress possible. T2 - 6. Meeting of the ISHAM Working Group "Black Yeasts and Relatives" CY - Viterbo, Italy DA - 15.09.2016 KW - Fungi KW - SAB KW - Biofilm PY - 2016 AN - OPUS4-37694 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Breitenbach, Romy A1 - Toepel, Jörg A1 - Dementyeva, Polina A1 - Knabe, Nicole A1 - Gorbushina, Anna ED - Flemming, H.-C. ED - Neu, T. R. ED - Wingener, J. T1 - Snapshots of fungal extracellular matrices N2 - Fungal extracellular materials reinforce a constant interaction between their cell wall and the environment. A dynamic mixture of chitin, glucans, mannans, glycoproteins, glycolipids and pigments supports the success of all fungal life styles – from symbiotic to the free-living and pathogenic. Fungi are perfectly adapted to grow on surfaces and in porous environments, where they form medically and geochemically relevant biofilms. Fungal EPS are critical in adhesion to other fungi, other cells or substratum as well as in the following interaction with the host immune system or material they attack, degrade and deteriorate respectively. Characterisation of extracellular compounds and understanding of its function is necessary to limit damage caused by fungal activity. All necessary methodology from chemical characterization to complete genetic analyses has been developed for medically important fungi. Now it is time to apply this knowledge to the numerous, largely aerobic and very active organisms that occupy a wide range of atmosphere-exposed habitats in the upper lithosphere. One can expect that analogies between medically- and environmentally-relevant model fungal species will help us to address the dynamics of the fungal cell EPS matrix in much more efficient and widely applicable ways. KW - Fungal cell wall KW - Melanin KW - Fungal life styles KW - Environmental and pathogenic fungi KW - Model fungal biofilms KW - Biogenic weathering PY - 2016 SN - 9781780407418 SN - 9781780407425 U6 - https://doi.org/10.2166/9781780407418 SP - Chapter 14, 269 EP - 299 PB - IWA Publishing CY - London, UK AN - OPUS4-38094 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Nicole T1 - Oxidative stress response of refractive ascomycetes: genetic manipulation of the rock-inhabiting model fungus Knufia petricola A95 T2 - Microbiology and Infection 2014; Jahrestagung; 4. gemeinsame Kongress DGHM/VAAM CY - Dresden, Germany DA - 2014-10-05 PY - 2014 AN - OPUS4-32166 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Nicole A1 - Gorbushina, Anna T1 - Roles of protective pigments in oxidative stress responses of the rock-inhabiting model fungus Knufia petricola A95 T2 - 28th Fungal Genetics Conference CY - Pacific Grove, California, USA DA - 2015-03-17 PY - 2015 AN - OPUS4-33057 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -