TY - CONF A1 - Koch, Matthias T1 - New reference materials for mineral oil analysis in water, soil and waste T2 - Analytica Conference 2004 (Poster des Consortiums vom EU-Projekt HYCREF (Vh 1275)) CY - Munich, Germany DA - 2004-05-11 PY - 2004 AN - OPUS4-5478 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias T1 - Ergebnisse zum TNS-Verbundprojekt ZENOL T2 - Sitzung des DIN/NAL Gesamtaustausches "Fette und Öle" CY - Hamburg DA - 2015-03-20 PY - 2015 AN - OPUS4-32857 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Brodehl, Antje A1 - Möller, Anne A1 - Kunte, Hans-Jörg A1 - Koch, Matthias A1 - Maul, Ronald T1 - Biotransformation of the mycotoxin zearalenone by fungi of the genera Rhizopus and Aspergillus N2 - Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin biosynthesized by various Fusarium fungi. These fungal species frequently infest grains; therefore, ZEN represents a common contaminant in cereal products. The biotransformation of ZEN differs significantly from species to species, and several metabolites are known to be formed by animals, plants, and microorganisms. The aim of the present study was to investigate the microbial conversion of ZEN by species of the genera Rhizopus and Aspergillus representing relevant fungi for food processing (e.g. fermentation). To monitor the ZEN metabolism, ZEN was added to liquid cultures of the different fungal species. After a period of 3 days, the media were analyzed by HPLC-MS/MS for metabolite formation. Two Aspergillus oryzae strains and all seven Rhizopus species were able to convert ZEN into various metabolites, including ZEN-14-sulfate as well as ZEN-O-14- and ZEN-O-16-glucoside. Microbial transformation of ZEN into the significantly more estrogenic α-zearalenol (α-ZEL) was also observed. Additionally, a novel fungal metabolite, α-ZEL-sulfate, was detected. Semi-quantification of the main metabolites indicates that more than 50% of initial ZEN may be modified. The results show that fungal strains have the potential to convert ZEN into various metabolites leading to a masking of the toxin, for example in fermented food. KW - Microbial conversion KW - Metabolites KW - Fermentation KW - Alpha-zearalenol KW - Conjugation KW - Mycotoxin biotransformation KW - Zearalenone-sulfate PY - 2014 DO - https://doi.org/10.1111/1574-6968.12586 SN - 0378-1097 SN - 1574-6968 VL - 359 IS - 1 SP - 124 EP - 130 PB - Wiley-Blackwell CY - Malden, Mass., USA AN - OPUS4-31636 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Keller, Julia A1 - Haase, Hajo A1 - Koch, Matthias T1 - Electrochemical simulation of biotransformation reactions of citrinin and dihydroergocristine compared to UV irradiation and Fenton-like reaction N2 - Mycotoxins occur widely in foodstuffs and cause a variety of mold-related health risks to humans and animals. Elucidation of the metabolic fate of mycotoxins and the growing number of newly discovered mycotoxins have enhanced the demand for fast and reliable simulation methods. The viability of electrochemistry coupled with mass spectrometry (EC/ESI-MS), Fenton-like oxidation, and UV irradiation for the simulation of oxidative phase I metabolism of the mycotoxins citrinin (CIT) and dihydroergocristine (DHEC) was investigated. The specific reaction products are compared with metabolites produced by human and rat liver microsomes in vitro. Depending on the applied potential between 0 and 2000 mV vs. Pd/H-2 by using a flow-through cell, CIT and DHEC are oxidized to various products. Besides dehydrogenation and dealkylation reactions, several hydroxylated DHEC and CIT species are produced by EC and Fenton-like reaction, separated and analyzed by LC-MS/MS and ESI-HRMS. Compared to reaction products from performed microsomal incubations, several mono- and dihydroxylated DHEC species were found to be similar to the reaction products of EC, Fenton-like reaction, and UV-induced oxidation. Consequentially, nonmicrosomal efficient and economic simulation techniques can be useful in early-stage metabolic studies, even if one-to-one simulation is not always feasible. KW - Mycotoxins KW - In vitro KW - Electrochemistry KW - Oxidation PY - 2017 DO - https://doi.org/10.1007/s00216-017-0350-6 SN - 1618-2642 VL - 409 IS - 16 SP - 4037 EP - 4045 PB - Springer CY - Heidelberg AN - OPUS4-40492 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias T1 - Certified reference materials for Ochratoxin A in coffee and wine T2 - Mykotoxin-Workshop CY - Utrecht, Netherlands DA - 2008-04-28 PY - 2008 AN - OPUS4-17494 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias T1 - European Reference Materials for Ochratoxin A in Coffee and Wine T2 - 38. Deutscher Lebensmittelchemikertag CY - Berlin, Germany DA - 2009-09-14 PY - 2009 AN - OPUS4-19962 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias A1 - Wöhrlin, F. T1 - Zwischenstand aus dem Verbundprojekt ZENOL T2 - 28. Sitzung der BVL-Arbeitsgruppe § 64 LFGB "Mykotoxine" CY - Berlin DA - 2014-11-26 PY - 2014 AN - OPUS4-32441 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Köppen, Robert A1 - Koch, Matthias A1 - Klein-Hartwig, Karin A1 - Nehls, Irene T1 - Natürliche Umwelt- und Lebensmitteltoxine: Herstellung eines Referenzmaterials für die Mykotoxine T-2 und HT-2 PY - 2010 SN - 0934-3504 SN - 1865-5084 VL - 22 IS - 4 SP - 407 PB - Springer CY - Heidelberg AN - OPUS4-22205 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Koch, Matthias A1 - Neuhof, Torsten A1 - Nehls, Irene ED - Jörg Peter Matthes, ED - Markus Frasch, ED - Marx, Wolfram ED - Johannes Oeler, T1 - Lebensmittelanalytik - Neue Referenzmaterialien KW - Referenzmaterialien KW - Acrylamid KW - Ochratoxin A KW - Methylquecksilber PY - 2007 VL - 3 IS - 5 SP - 16 EP - 17 PB - Succidia Verl. & Kommunikation CY - Darmstadt AN - OPUS4-16691 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - THES A1 - Koch, Matthias T1 - Entwicklung und Vergleich von Analysenmethoden zur Bestimmung von Mineralölkohlenwasserstoffen in Wasser und in Böden PY - 1999 SP - 1 EP - 154 CY - Berlin AN - OPUS4-7042 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias T1 - Stand der Normung - Bestimmung von Mineralölkohlenwasserstoffen (MKW) mittels GC-FID in verschiedenen Matrices (Wasser, Boden, Abfall) T2 - DGMK-Analytikertreffen 2000 CY - Hamburg, Deutschland DA - 2000-11-13 PY - 2000 CY - Hamburg AN - OPUS4-1031 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias T1 - Development of certified reference materials for the determination of mineral oil hydrocarbons in water, soil and waste T2 - EU-Projekt HYCREF für die BERM-9 CY - Berlin, Germany DA - 2003-06-17 PY - 2003 AN - OPUS4-5856 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Koch, Matthias T1 - Vergleich von IR- und GC-Verfahren zur Bestimmung von Mineralölkohlenwasserstoffen (MKW) in Waseer T2 - Auswertungsveranstaltung zum 19. und 20. VGS-Ringversuch "Organische Parameter"; BAM/DAP CY - Berlin, Germany DA - 2000-03-16 PY - 2000 AN - OPUS4-5753 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Koch, Matthias A1 - Jancke, Harald A1 - Nehls, Irene T1 - Messung und Quantifizierung von Mineralölkohlenwasserstoffen - Ein Vergleich von FT/IR, GC-FID und 1H-NMR KW - H-1-Kernresonanz-Spektroskopie KW - H-1-NMR KW - Fourier-Transform-Infrarot-Spektroskopie KW - FT/IR KW - GC-FID KW - Kapillargaschromatographie-Flammenionisationsdetektion KW - MKW KW - Mineralölkohlenwasserstoffe KW - Umweltanalytik PY - 1999 SN - 0934-3504 SN - 1865-5084 VL - 11 IS - 1 SP - 9 EP - 14 PB - Springer CY - Heidelberg AN - OPUS4-7041 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -