TY - CONF A1 - Hahn, Marc Benjamin T1 - Near-ambient-pressure XPS as as tool to monitor DNA radiation damage directly in water N2 - Ionizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. T2 - Miller Conference CY - Furiani, France DA - 03.06.2023 KW - Base damage KW - Base loss KW - Cancer treatment KW - DNA KW - DNA radiation damage KW - Direct damage KW - Dissociative electron attachment (DEA) KW - Dissociative electron transfer (DET) KW - Dosimetry KW - Double-strand break (DSB) KW - ESCA KW - Energy deposit KW - G5P KW - Geant4 KW - Geant4-DNA KW - Hydrated DNA KW - Hydrated electron KW - Hydroxyl radical KW - Indirect damage KW - Ionisation KW - Ionization KW - LEE KW - Low energy electrons KW - MCS KW - Microdosimetry KW - NAP-XPS KW - Near ambient pressure xray photo electron spectroscopy KW - Net-ionization reaction KW - OH radical KW - PES KW - Particle scattering KW - Prehydrated electron KW - Presolvated electron KW - Protein KW - Quasi-direct damage KW - ROS KW - Radiation damage KW - Radiation therapy KW - Radical KW - Radiolysis KW - Radiotherapy KW - Reactive oxygen species KW - Simulation KW - Single-strand break (SSB) KW - Single-stranded DNA-binding proteins KW - TOPAS KW - TOPAS-nbio KW - XPS KW - Xray KW - Xray photo electron spectrocopy KW - Hydration shell PY - 2023 AN - OPUS4-57646 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hahn, Marc Benjamin T1 - BP153: The change of DNA radiation damage upon hydration: In-situ observation by near-ambient pressure XPS N2 - Ionizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. T2 - DPG Frühjahrstagung CY - Dresden, Germany DA - 26.03.2023 KW - Base damage KW - Base loss KW - Cancer treatment KW - DNA KW - DNA radiation damage KW - Direct damage KW - Dissociative electron attachment (DEA) KW - Dissociative electron transfer (DET) KW - Dosimetry KW - Double-strand break (DSB) KW - Energy deposit KW - Geant4 KW - Geant4-DNA KW - Hydrated DNA KW - Hydrated electron KW - Hydration shell KW - Hydroxyl radical KW - Indirect damage KW - Ionization KW - LEE KW - Low energy electrons KW - MCS KW - Microdosimetry KW - NAP-XPS KW - Near ambient pressure xray photo electron spectroscopy KW - Net-ionization reaction KW - OH radical KW - PES KW - Prehydrated electron KW - Quasi-direct damage KW - ROS KW - Radiation damage KW - Radiation therapy KW - Radical KW - Radiolysis KW - Radiotherapy KW - Reactive oxygen species KW - Simulation KW - Single-strand break (SSB) KW - TOPAS KW - TOPAS-nbio KW - XPS KW - Xray KW - Xray photo electron spectrocopy KW - Particle scattering KW - Presolvated electron KW - ESCA PY - 2023 AN - OPUS4-57255 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hahn, Marc Benjamin T1 - Near-Ambient-Pressure XPS to investigate radiation damage to DNA N2 - X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. Our results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. T2 - Physical and Chemical Analysis of Polymers seminar CY - Online meeting DA - 12.10.2021 KW - Base damage KW - Base loss KW - Cancer therapy KW - DNA KW - DNA radiation damage KW - Direct damage KW - Dissociative electron attachment (DEA) KW - Dissociative electron transfer (DET) KW - Dosimetry KW - Double-strand break KW - DSB KW - Dry DNA KW - Geant4 KW - Geant4-DNA KW - Hydrated DNA KW - Hydrated electron KW - Hydration shell KW - Hydroxyl radical KW - Indirect damage KW - Ionization KW - LEE KW - Low energy electrons KW - Microdosimetry KW - NAP-XPS KW - Near ambient pressure xray photo electron spectroscopy KW - Net-ionization reaction KW - OH radical KW - PES KW - Prehydrated electron KW - Quasi-direct damage KW - ROS KW - Radiation damage KW - Radiation therapy KW - Radical KW - Reactive oxygen species KW - Single-strand break KW - SSB KW - TOPAS KW - TOPAS-nbio KW - XPS KW - Xray KW - Xray photo electron spectrocopy PY - 2021 AN - OPUS4-53611 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pauw, Brian Richard A1 - Smales, Glen Jacob A1 - Anker, A. S. A1 - Annadurai, V. A1 - Balazs, D. M. A1 - Bienert, Ralf A1 - Bouwman, W. G. A1 - Breßler, Ingo A1 - Breternitz, J. A1 - Brok, E. S. A1 - Bryant, G. A1 - Clulow, A. J. A1 - Crater, E. R. A1 - De Geuser, F. A1 - Del Giudice, A. A1 - Deumer, J. A1 - Disch, S. A1 - Dutt, S. A1 - Frank, K. A1 - Fratini, E. A1 - Garcia, P. R. A. F. A1 - Gilbert, E. P. A1 - Hahn, Marc Benjamin A1 - Hallett, J. A1 - Hohenschutz, M. A1 - Hollamby, M. A1 - Huband, S. A1 - Ilavsky, J. A1 - Jochum, J. K. A1 - Juelsholt, M. A1 - Mansel, B. W. A1 - Penttilä, P. A1 - Pittkowski, R. K. A1 - Portale, G. A1 - Pozzo, L. D. A1 - Rochels, L. A1 - Rosalie, Julian A1 - Saloga, Patrick E. J. A1 - Seibt, S. A1 - Smith, A. J. A1 - Smith, G. N. A1 - Spiering, G. A. A1 - Stawski, Tomasz M. A1 - Taché, O. A1 - Thünemann, Andreas A1 - Toth, K. A1 - Whitten, A. E. A1 - Wuttke, J. T1 - The human factor - Results of a small-angle scattering data analysis round robin N2 - A round-robin study has been carried out to estimate the impact of the human element in small-angle scattering data analysis. Four corrected datasets were provided to participants ready for analysis. All datasets were measured on samples containing spherical scatterers, with two datasets in dilute dispersions and two from powders. Most of the 46 participants correctly identified the number of populations in the dilute dispersions, with half of the population mean entries within 1.5% and half of the population width entries within 40%. Due to the added complexity of the structure factor, far fewer people submitted answers on the powder datasets. For those that did, half of the entries for the means and widths were within 44 and 86%, respectively. This round-robin experiment highlights several causes for the discrepancies, for which solutions are proposed. KW - Round Robin KW - Data analysis KW - Small-angle scattering KW - Nanomaterials KW - Interlaboratory comparability KW - Nanostructure quantification KW - Methodology KW - MOUSE PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-587091 DO - https://doi.org/10.1107/S1600576723008324 SN - 1600-5767 VL - 56 IS - 6 SP - 1618 EP - 1629 PB - Wiley-Blackwell CY - Oxford AN - OPUS4-58709 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - INPR A1 - Pauw, Brian Richard A1 - Smales, Glen Jacob A1 - Anker, A. S. A1 - Balazs, D. M. A1 - Beyer, F. L. A1 - Bienert, Ralf A1 - Bouwman, W. G. A1 - Breßler, Ingo A1 - Breternitz, J. A1 - Brok, E. S. A1 - Bryant, G. A1 - Clulow, A. J. A1 - Crater, E. R. A1 - De Geuser, F. A1 - Giudice, A. D. A1 - Deumer, J. A1 - Disch, S. A1 - Dutt, S. A1 - Frank, K. A1 - Fratini, E. A1 - Gilbert, E. P. A1 - Hahn, Marc Benjamin A1 - Hallett, J. A1 - Hohenschutz, Max A1 - Hollamby, M. J. A1 - Huband, S. A1 - Ilavsky, J. A1 - Jochum, J. K. A1 - Juelsholt, M. A1 - Mansel, B. W. A1 - Penttilä, P. A1 - Pittkowski, R. K. A1 - Portale, G. A1 - Pozzo, L. D. A1 - Ricardo de Abreu Furtado Garcia, P. A1 - Rochels, L. A1 - Rosalie, Julian A1 - Saloga, P. E. J. A1 - Seibt, S. A1 - Smith, A. J. A1 - Smith, G. N. A1 - Annadurai, V. A1 - Spiering, G. A. A1 - Stawski, Tomasz A1 - Taché, O. A1 - Thünemann, Andreas A1 - Toth, K. A1 - Whitten, A. E. A1 - Wuttke, J. T1 - The human factor - Results of a small-angle scattering data analysis round robin N2 - A Round Robin study has been carried out to estimate the impact of the human element in small-angle scattering data analysis. Four corrected datasets were provided to participants ready for analysis. All datasets were measured on samples containing spherical scatterers, with two datasets in dilute dispersions, and two from powders. Most of the 46 participants correctly identified the number of populations in the dilute dispersions, with half of the population mean entries within 1.5 % and half of the population width entries within 40 %, respectively. Due to the added complexity of the structure factor, much fewer people submitted answers on the powder datasets. For those that did, half of the entries for the means and widths were within 44 % and 86 % respectively. This Round Robin experiment highlights several causes for the discrepancies, for which solutions are proposed. KW - Round robin KW - Sall-angle scattering KW - Nanostructure quantification KW - Nanostructure KW - SAXS KW - MOUSE KW - X-ray scattering KW - Size distribution KW - Nanoparticles PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-571342 DO - https://doi.org/10.48550/arXiv.2303.03772 SP - 1 EP - 23 PB - Cornell University CY - Ithaca, NY AN - OPUS4-57134 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hahn, Marc Benjamin T1 - Near-ambient-pressure Xray photoelectron spectroscopy (XPS) to monitor DNA radiation damage directly in water N2 - Ionizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. T2 - RADeleven CY - Herceg Novi, Montenegro DA - 19.06.2023 KW - Base damage KW - Base loss KW - Cancer treatment KW - DNA KW - DNA radiation damage KW - Direct damage KW - Dissociative electron attachment (DEA) KW - Dissociative electron transfer (DET) KW - Dosimetry KW - Double-strand break (DSB) KW - ESCA KW - Energy deposit KW - Geant4 KW - Geant4-DNA KW - Hydrated DNA KW - Hydrated electron KW - Hydrated electrons KW - Hydration shell KW - Hydroxyl radical KW - Indirect damage KW - Ionisation KW - Ionization KW - LEE KW - DEA KW - DET KW - Low energy electrons KW - MCS KW - Microdosimetry KW - NAP-XPS KW - Near ambient pressure xray photo electron spectroscopy KW - Net-ionization reaction KW - OH radical KW - PES KW - Particle scattering KW - Prehydrated electron KW - Presolvated electron KW - Protein KW - Quasi-direct damage KW - ROS KW - Radiation damage KW - Radiation therapy KW - Radical KW - Radiolysis KW - Radiotherapy KW - Reactive oxygen species KW - Simulation KW - Single-strand break (SSB) KW - Single-stranded DNA-binding proteins KW - TOPAS KW - TOPAS-nbio KW - XPS KW - Xray photo electron spectrocopy PY - 2023 AN - OPUS4-57782 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hahn, Marc Benjamin T1 - The change of DNA AND PROTEIN radiation damage upon hydration: In-situ observations by near-ambient-pressure XPS N2 - X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. Our results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. T2 - Dyson Conference 2023 CY - Prague, Czech Republic DA - 24.04.2023 KW - Base damage KW - Base loss KW - Cancer treatment KW - DNA KW - DNA radiation damage KW - Direct damage KW - Dissociative electron attachment (DEA) KW - Dissociative electron transfer (DET) KW - Dosimetry KW - Double-strand break (DSB) KW - ESCA KW - Energy deposit KW - Geant4 KW - Geant4-DNA KW - Hydrated DNA KW - Hydrated electron KW - Hydration shell KW - Hydroxyl radical KW - Indirect damage KW - Ionization KW - Ionisation KW - LEE KW - Low energy electrons KW - MCS KW - Microdosimetry KW - NAP-XPS KW - Near ambient pressure xray photo electron spectroscopy KW - Net-ionization reaction KW - OH radical KW - PES KW - Particle scattering KW - Prehydrated electron KW - Presolvated electron KW - Quasi-direct damage KW - ROS KW - Radiation damage KW - Radiation therapy KW - Radical KW - Radiolysis KW - Radiotherapy KW - Reactive oxygen species KW - Simulation KW - Single-strand break (SSB) KW - TOPAS KW - TOPAS-nbio KW - XPS KW - Xray KW - Xray photo electron spectrocopy KW - G5P KW - Protein KW - Single-stranded DNA-binding proteins PY - 2023 AN - OPUS4-57406 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hahn, Marc Benjamin T1 - The change of DNA radiation damage upon hydration: In-situ observations by near-ambient-pressure XPS N2 - Ionizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. T2 - #RSCposter 2023 CY - Online meeting DA - 28.02.2023 KW - Cancer treatment KW - DNA KW - Dosimetry KW - Energy deposit KW - Geant4 KW - Geant4-DNA KW - TOPAS KW - TOPAS-nbio KW - particle scattering KW - Simulation KW - Radiolysis KW - Radiation therapy KW - Radiotherapy KW - LEE KW - Low energy electrons KW - MCS KW - Base damage KW - Base loss KW - DNA radiation damage KW - Direct damage KW - Dissociative electron transfer (DET) KW - Dissociative electron attachment (DEA) KW - Double-strand break (DSB) KW - Hydrated DNA KW - Hydrated electron KW - Ionization KW - Hydration shell KW - Hydroxyl radical KW - Indirect damage KW - Microdosimetry KW - NAP-XPS KW - Near ambient pressure xray photo electron spectroscopy KW - Net-ionization reaction KW - OH radical KW - PES KW - Prehydrated electron KW - Quasi-direct damage KW - Radiation damage KW - Radical KW - Reactive oxygen species KW - ROS KW - Single-strand break (SSB) KW - XPS KW - Xray KW - Xray photo electron spectrocopy KW - presolvated electron PY - 2023 UR - https://www.nature.com/articles/s42004-021-00487-1 AN - OPUS4-57063 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hahn, Marc Benjamin T1 - The change of dna and protein radiation damage upon hydration: in-situ observations by near-ambient-pressure xps N2 - X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS experiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. Our results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further. Furthermore, first data about the degradation of single-stranded DNA binding-proteins (G5P / GV5 and hmtSSB) under vacuum and NAP-XPS conditions are presented. T2 - AVS69 CY - Portland, USA DA - 05.11.2023 KW - Base damage KW - Base loss KW - Cancer treatment KW - DNA KW - Protein KW - Proteins KW - Geant4 KW - Dosimetry KW - Microdosimetry KW - NAP-XPS KW - Xray photo electron spectrocopy KW - Radiation damage KW - Geant4-DNA KW - G5P KW - GVP KW - Hydroxyl radical KW - LEE KW - DEA KW - DET KW - ROS KW - Prehydrated electron KW - TOPAS KW - Near ambient pressure xray photo electron spectroscopy KW - SSB KW - DSB KW - Single-strand break (SSB) KW - ESCA KW - Single-stranded DNA-binding proteins KW - Reactive oxygen species PY - 2023 AN - OPUS4-58761 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -