TY - JOUR A1 - Nourinejhad Zarghani, S. A1 - Monavari, Mehran A1 - Nourinejhad Zarghani, A. A1 - Nouri, S. A1 - Ehlers, J. A1 - Hamacher, J. A1 - Bandte, M. A1 - Büttner, C. T1 - Quantifying plant viruses: Evolution from bioassay to infectivity dilution curves along the model of tobamoviruses N2 - This review describes the development of the bioassay as a means of quantifying plant viruses, with particular attention to tobamovirus. It delves into various models used to establish a correlation between virus particle concentration and the number of induced local lesions (the infectivity dilution curve), including the Poisson, Furumoto and Mickey, Kleczkowski, Growth curve, and modified Poisson models. The parameters of each model are described, and their application or performance in the context of the tobacco mosaic virus is explored. This overview highlights the enduring value of the infectivity dilution curve in tobamovirus quantification, providing valuable insights for researchers or practitioners of bioassays and theoreticians of modeling. KW - Local lesion KW - qRT-PCR KW - Nicotiana KW - ToBRFV KW - TMV KW - CGMMV KW - Disinfection KW - Modeling PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-630142 DO - https://doi.org/10.3390/v16030440 SN - 1999-4915 VL - 16 IS - 3 SP - 1 EP - 14 PB - MDPI CY - Basel AN - OPUS4-63014 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -