TY - JOUR A1 - Würth, Christian A1 - Lochmann, Cornelia A1 - Spieles, Monika A1 - Pauli, Jutta A1 - Hoffmann, Katrin A1 - Schüttrigkeit, T. A1 - Franzl, T. A1 - Resch-Genger, Ute T1 - Evaluation of a commercial integrating sphere setup for the determination of absolute photoluminescence quantum yields of dilute dye solutions JF - Applied spectroscopy N2 - The commercial availability of stand-alone setups for the determination of absolute photoluminescence quantum yields (φf) in conjunction with the increasing use of integrating sphere accessories for spectrofluorometers is expected to have a considerable influence not only on the characterization of chromophore systems for use in optical and opto-electronic devices, but also on the determination of this key parameter for (bio)analytically relevant dyes and functional luminophores. Despite the huge potential of systems measuring absolute φf values and the renewed interest in dependable data, evaluated protocols for even the most elementary case, the determination of the fluorescence quantum yield of transparent dilute solutions of small organic dyes with integrating sphere methods, are still missing. This encouraged us to evaluate the performance and sources of uncertainty of a simple commercial integrating sphere setup with dilute solutions of two of the best characterized fluorescence quantum yield standards, quinine sulfate dihydrate and rhodamine 101, strongly differing in spectral overlap between absorption and emission. Special attention is dedicated to illustrate common pitfalls of this approach, thereby deriving simple procedures to minimize measurement uncertainties and improve the comparability of data for the broad community of users of fluorescence techniques. KW - Lifetime KW - Fluorescence KW - Luminescence KW - Quantum yield KW - Quantum efficiency KW - Integrating sphere KW - Reabsorption KW - Rhodamine 101 KW - Quinine sulfate dihydrate KW - Method KW - Photoluminescence KW - Standard KW - Emission KW - Spectral correction KW - Excitation KW - Anisotropy PY - 2010 DO - https://doi.org/10.1366/000370210791666390 SN - 0003-7028 SN - 1943-3530 VL - 64 IS - 7 SP - 733 EP - 741 PB - Society for Applied Spectroscopy CY - Frederick, Md. AN - OPUS4-22089 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -