TY - CONF A1 - Lischka, Susanne A1 - Piechotta, Christian A1 - Körte, F. T1 - Arsenobetainanalytik unter Einsatz eines 13C-markierten Kalibrierstandard für Elektrospray-Massenspektrometrie T2 - Doktorandenseminar des AK Separation Science der GDCh CY - Hohenroda, Germany DA - 2012-01-08 PY - 2012 AN - OPUS4-25662 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Lischka, Susanne A1 - Arroyo-Abad, Uriel A1 - Mattusch, J. A1 - Kühn, Andreas A1 - Piechotta, Christian T1 - The high diversity of arsenolipids in herring fillet (Clupea harengus) N2 - Arsenolipids represent a relevant step in the biosynthesis of organoarsenicals from inorganic arsenic compounds. Their fate after human consumption is still uncertain. By means of a HPLC-ICP-MS/ESI-Q-TOF-MS method, 16 lipid soluble arsenic compounds, including seven formerly unknown organoarsenicals, have been identified in commercial herring fillet. The structural assignment was done by exact mass and high resolution MS/MS data. This is the first identification of arsenolipids in herring (Clupea harengus). They contribute with (3.6±0.2) mg kg-1 arsenic to 62.3% of the total arsenic content of (5.7±0.3) mg of arsenic per kg dry mass. Current studies indicate that a metabolization by humans to cancerous dimethylarsinic acid is very likely. The presented results are highly relevant as herring is a very popular food fish species in Europe. Moreover, the screening of different fish species revealed that arsenolipids are more widespread than previously assumed. KW - Arsenic species KW - Arsenolipids KW - Fish KW - Herring KW - ESI-MS/MS KW - ICP-TOF-MS PY - 2013 DO - https://doi.org/10.1016/j.talanta.2013.02.051 SN - 0039-9140 VL - 110 SP - 144 EP - 152 PB - Elsevier CY - Amsterdam AN - OPUS4-28302 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Lischka, Susanne A1 - Körte, F. A1 - Faust, R. A1 - Nehls, Irene A1 - Piechotta, Christian T1 - Synthesis, characterization and application of a two-fold 13C-labeled calibration standard for the analysis of arsenobetaine using HPLC-ESI-MS/MS without high resolution mass spectrometry N2 - A procedure has been developed for the determination of arsenobetaine in fish matrix by HPLC–ESI-MS/MS. Hereby (trimethylarsonium)-1,2-13C-acetate (arsenobetaine) is used as internal calibration standard. Arsenobetaine was determined in a fish material (Sea Bass) with an expanded uncertainty of 3.8%. KW - Arsenobetaine KW - ESI-MS/MS KW - Isotopic labeled KW - 13C PY - 2011 DO - https://doi.org/10.1016/j.talanta.2011.07.047 SN - 0039-9140 VL - 85 IS - 4 SP - 1996 EP - 1999 PB - Elsevier CY - Amsterdam AN - OPUS4-25163 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Piechotta, Christian T1 - Application of a twofold C-labeled calibration standard for the analysis of arsenobetaine using HPLC-ESI-MS/MS T2 - CIA CY - Berlin, Germany DA - 2011-09-26 PY - 2011 AN - OPUS4-24418 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Körte, F. A1 - Piechotta, Christian T1 - Arsenobetainbestimmung in Fisch mittels LC-ESI-MS/MS T2 - CIA CY - Berlin, Germany DA - 2011-09-26 PY - 2011 AN - OPUS4-24419 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Kaminski, Katja A1 - Klyk-Seitz, Urszula-Anna A1 - Win, Tin T1 - Zweidimensionale GC von Mineral-Kohlenwasserstoffen in Böden im Hinblick auf neue toxikologisch relevante Prüfwerte T2 - Shimadzu Anwendertreffen für multidimensionale Gaschromatographie CY - Leipzig, Germany DA - 2009-10-29 PY - 2009 AN - OPUS4-20227 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Piechotta, Christian T1 - Synthese und Reinheitsuntersuchungen 13C-markierter Standards für die Arsenspeziesanalytik mittels LC-ESI-MS T2 - 9. MS-Symposium CY - Berlin, Germany DA - 2010-09-06 PY - 2010 AN - OPUS4-21852 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Piechotta, Christian T1 - 13C-markierte Kalibrierstandards für die Arsenspeziesanalytik mittels LC-ESI-MS/MS T2 - Berliner Chemie Symposium (Jungchemikerforum) der Humboldt-Universität Berlin CY - Berlin, Germany DA - 2011-04-07 PY - 2011 AN - OPUS4-23504 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Piechotta, Christian T1 - Application of a 13C-lableled calibration standard for the analysis of arsenobetaine in fish using HPLC-ESI-MS/MS T2 - TraceSpec CY - Pau, France DA - 2011-05-18 PY - 2011 AN - OPUS4-23563 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Piechotta, Christian T1 - Isotopic labelled standard for the routine analysis of arenobetaine T2 - IICS - 24th International Ion Chromatography Symposium CY - Berlin, Germany DA - 2012-09-18 PY - 2012 AN - OPUS4-26569 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Richter, Janine A1 - Lischka, Susanne A1 - Piechotta, Christian T1 - Analysis of arsenic species in fish after derivatization by GC-MS N2 - The derivatization of organoarsenic compounds by different reagents like thioglycolates or dithiols and the subsequent analysis by GC–MS as a molecular specific technique was investigated and described. The possible derivatization reagents methyl- and ethylthioglycolate (TGM and TGE), 1,3-propane- and 1,5-pentanedithiol (PDT and PeDT), which transfer the polar and nonvolatile analytes dimethylarsenate (DMA), monomethylarsonate (MMA), arsenite and arsenate into volatile compounds, were evaluated. The application for real samples like fish material was also studied. In addition the gas chromatographic separation and resolution was optimized and experiments were carried out to determine the highest derivatization rates. Derivatization reagents were evaluated in terms of quantity and stability of the formed chemical species. All derivatization products were characterized by mass spectrometry in order to identify the separated arsenic species. The most efficient conversion of DMA and MMA was observed by using ethylthioglycolate as derivatization agent. Finally, the derivatization procedure and the GC–MS-method were validated to determine linearity, precision, selectivity, analytical limiting values and recoveries. For the proposed method a limit of detection (LOD) of 5.8 pg for DMA and 14.0 pg for MMA was found. The accuracy was established by comparing the mean value measured for DMA in the certified reference material BCR-627 (tuna fish) with the certified one. MMA was not quantified in marine samples due to its low content. In shrimp samples DMA was not detectable. For codfish a DMA-content of 0.20±0.004 mg kg-1, for 'Surströmming' an amount of 0.38±0.02 mg kg-1 and for herring, which showed the highest amount of DMA, a content of 1.15±0.03 mg kg-1 was determined. KW - Arsenic species analysis KW - Monomethylarsonate KW - Dimethylarsenate KW - Derivatization KW - Gas chromatography KW - Mass spectrometry PY - 2012 DO - https://doi.org/10.1016/j.talanta.2012.10.021 SN - 0039-9140 VL - 101 SP - 524 EP - 529 PB - Elsevier CY - Amsterdam AN - OPUS4-26931 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Piechotta, Christian T1 - Einsatz eines isotopenmarkierten Kalibrierstandards zur Bestimmung von Arsenobetain mittels HPLC-ESI-MS/MS T2 - Kolloquium "LC-MS in der Umweltanalytik 2012" CY - Leipzig, Germany DA - 2012-06-11 PY - 2012 AN - OPUS4-26124 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Arroyo-Abad, Uriel A1 - Lischka, Susanne A1 - Piechotta, Christian A1 - Mattusch, J. A1 - Reemtsma, T. T1 - Determination and identification of hydrophilic and hydrophobic arsenic species in methanol extract of fresh cod liver by RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection N2 - The present study was focused on the determination and identification of arsenic species in methanolic extracts of cod liver. Arsenic species were fractionated and the fractions analysed by RP-HPLC-ICP-MS coupled with ESI-Q-TOF-MS. The total concentration of arsenic in the fresh cod liver was analysed by ICP-MS to be 1.53 ± 0.02 mg As kg-1 w.w. and the extraction recovery was ca. 100% and the column recovery >93%. Besides polar inorganic and methylated arsenic species (>70%) more hydrophobic arsenic-containing fatty acids and hydrocarbons occurred. Based on the mass spectrometric data proposals for molecular structures were elaborated for 20 of the organic As species included 10 arsenic-containing fatty acids (AsFA) and an arsenic-containing hydrocarbon (AsHC) mentioned for the first time in fresh cod liver. Arsenobetaine was found as main water-soluble arsenic compound in cod liver followed by higher molecular mass arsenic-containing fatty acids and hydrocarbons. KW - Arsenic species KW - Arsenolipids KW - Marine fish KW - Cod liver KW - HPLC-ICP-MS/ESI-Q-TOF-MS PY - 2013 DO - https://doi.org/10.1016/j.foodchem.2013.05.152 SN - 0308-8146 VL - 141 IS - 3 SP - 3093 EP - 3102 PB - Elsevier CY - Amsterdam [u.a.] ; Jena AN - OPUS4-28852 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Brenneis, Rudolf A1 - Moritz, Jan A1 - Adam, Christian T1 - Enzyme-catalyzed synthesis of bio-lubricants from waste fat T2 - BioCat 2014 CY - Hamburg, Germany DA - 2014-08-31 PY - 2014 AN - OPUS4-32101 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Piechotta, Christian T1 - Development of certified isotopic calibrants for the analysis of organic arsenic species with LC-ESI-MS T2 - The Future of Reference Materials - Science and Innovation CY - Geel, Belgium DA - 2010-11-23 PY - 2010 AN - OPUS4-22456 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Piechotta, Christian T1 - 13C-markierte Kalibrierstandards für die Arsenspeziesanalytik mittels LC-ESI-MS/MS T2 - CANAS CY - Leipzig, Germany DA - 2011-03-13 PY - 2011 AN - OPUS4-23368 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Janzen, R. A1 - Sperling, M. A1 - Buscher, W. A1 - Bösel, A. A1 - Ehlbeck, J. A1 - Lischka, Susanne A1 - Piechotta, Christian T1 - Spezies-Analyse von Quecksilber-, Zinn- und Bleiverbindungen - Neues GC-MIP-OES-System N2 - Quecksilber-, Blei- und Zinnverbindungen gehören aufgrund Ihrer Toxizität zu den bedrohlichsten Umweltkontaminationen. Dabei können besonders die organischen Verbindungen bereits in kleinsten Mengen überaus schwerwiegende Folgen für Lebewesen verursachen. Während die anorganischen (mineralischen) Verbindungen dieser Elemente auch durch natürliche Prozesse wie Erosion aus Gestein und durch Vulkanismus in die Umwelt eingetragen werden, entstehen die meisten Kontaminationen der Umwelt aufgrund ihres jahrzehntelangen weltweiten Einsatzes in verschiedenen Pestiziden, Antifouling Anstrichen, Treibstoffen und zahlreichen industriellen Großprozessen, sowie ihrer Emission bei der Verfeuerung fossiler Brennstoffe. KW - GC-PED KW - Methylquecksilber KW - MIP PY - 2011 SN - 0016-3538 VL - 1 SP - 22 EP - 27 PB - GIT-Verlag CY - Darmstadt AN - OPUS4-23374 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Piechotta, Christian A1 - Win, Tin A1 - Nehls, Irene T1 - Synthese isotopenmarkierter, interner Standards für die Bestimmung von Arsenspezies in Biota mittels LC-ESI-MS T2 - Tag der Chemie, FU Berlin CY - Berlin, Germany DA - 2010-06-16 PY - 2010 AN - OPUS4-21420 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lischka, Susanne A1 - Nehls, Irene A1 - Panne, Ulrich A1 - Piechotta, Christian T1 - Synthese 13C-markierter Kalibrierstandards für die Arsenspeziesanalytik mittels LC-ESI-MS/MS T2 - ANAKON 2011 CY - Zurich, Switzerland DA - 2011-03-22 PY - 2011 AN - OPUS4-23369 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -