TY - GEN A1 - Krüger, Ralph-Peter A1 - Heinemann, M. A1 - Falkenhagen, Jana A1 - Hartmann, A. A1 - Friedrich, Jörg Florian A1 - Fritz, H.-G. ED - Fritz, H. ED - Eisenbach, C. T1 - Charakterisierung biologisch abbaubarer Homo- und Copolyester / Characterisation of biodegradable Homo- and Copolyesters T2 - 19. Stuttgarter Kunststoff-Kolloquium 2005 CY - Stuttgart, Deutschland DA - 2005-03-09 KW - MALDI-TOF-MS KW - Bioabbaubare Polyester KW - Flüssigchromatographie KW - Methodenkopplung KW - Biodegradable polyesters KW - Liquid chromatography KW - Coupling of analytical methods PY - 2005 SN - 3-00-015246-6 SP - 5/P1-1 - 5/P1-9 PB - IKT CY - Stuttgart AN - OPUS4-11767 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hansen, H.-C. A1 - Lagrèze, W. A1 - Krüger, Oswald A1 - Helmke, K. T1 - Dependence of the optic nerve sheath diameter on acutely applied subarachnoidal pressure - an experimental ultrasound study N2 - Purpose: To determine the distensibility and elastic characteristics of the optic nerve sheath for development of a basic understanding of ultrasound studies aimed to measure optic nerve sheath diameter (ONSD) for detection of acutely elevated intracranial pressure (ICP). Methods: Isolated human optic nerves preparations obtained from autopsies were submitted to predefined pressure alterations, and consecutive changes in ONSD were measured by B-scan ultrasound under defined conditions. Results: Following submission to pressure, the diameter of the nerve sheath increased up to 140% of its baseline value. The increase (mean 1.97 mm, SD 0.52 mm) corresponded to the magnitude of pressure steps measured in the perineural subarachnoidal space (SAS). Similarly, the ONSD declined in each of the preparations within a few minutes after the optic nerve was decompressed. However, it did not reach its baseline value again when pressure loads of 45–55 mmHg or more had been applied. Conclusions: The elasticity of the anterior sheath of the optic nerve is sufficient for the detection of pressure changes in the SAS especially for upward pressure steps. This is basically important for the application of clinical monitoring of the sheath diameter to facilitate the identification of patients with elevated ICP non-invasively (screening). However, further implementation of this procedure in neurointensive care and emergency medicine has to consider that the sheath diameter reversibility may be impaired after episodes of prolonged intracranial hypertension and a model for hysteresis is proposed. KW - Intensive care KW - Intracranial pressure KW - Neuromonitoring KW - Optic nerve sheath KW - Ultrasound PY - 2011 DO - https://doi.org/10.1111/j.1755-3768.2011.02159.x SN - 1755-375X SN - 1755-3768 VL - 89 IS - 6 SP - e528 EP - e532 PB - Blackwell CY - Oxford AN - OPUS4-24017 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gappenach, C. A1 - Krauß, H.-J. A1 - Krüger, Jörg A1 - Offenhäuser, F. A1 - Pintaske, S. T1 - Auswahl Laserschutzprodukte - eine Handreichung N2 - Wer mit Laserstrahlung zu tun hat, sollte vor allem auf den Schutz seiner Augen achten, denn die Netzhaut ist eines der empfindlichsten Gewebe des menschlichen Körpers. Auch die Hornhaut des Auges kann durch Laserstrahlung gefährdet sein. Sie hat eine Dicke von nur etwa 0,5 mm. Der Schutz der Augen vor Laserstrahlung ist durch verschiedene Maßnahmen möglich. KW - Laserschutz KW - Schutzbrille KW - Justierbrille KW - Abschirmung KW - EN 207 KW - EN 208 KW - EN 12254 PY - 2015 SN - 1432-9778 VL - 3 SP - 40 EP - 45 PB - AT-Fachverl. CY - Fellbach AN - OPUS4-33431 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gappenach, C. A1 - Krüger, Jörg A1 - Offenhäuser, F. A1 - Pintaske, S. A1 - Krauß, H.-J. T1 - Selecting laser eye protectors - a helping hand N2 - The European laser safety standards EN 207, EN 208, and EN 12254 each contain an annex B, which serves as a guidance for the selection of products. These annexes are informative only and are therefore not binding. As there are a variety of hazard scenarios, it is not recommended to change these annexes to a normative status, through which they would become mandatory. Instead, it is recommended to allow users to apply their own skills and know-how in selecting appropriate products, justifying where and why they deviate from the guidance in the standards. This paper explains the background on which the guidance for selection in the annexes of the standards is based and shows physically meaningful leeway. KW - European standard KW - Laser safety KW - Personal protective equipment PY - 2015 DO - https://doi.org/10.1515/aot-2015-0043 SN - 2192-8576 SN - 2192-8584 VL - 4 IS - 5-6 SP - 389 EP - 395 PB - De Gruyter CY - Berlin; Boston, Mass. AN - OPUS4-35072 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ringleb, F. A1 - Eylers, K. A1 - Teubner, T. A1 - Schramm, H.-P. A1 - Symietz, Christian A1 - Bonse, Jörn A1 - Andree, Stefan A1 - Heidmann, B. A1 - Schmid, M. A1 - Krüger, Jörg A1 - Boeck, T. T1 - Growth and shape of indium islands on molybdenum at micro-roughened spots created by femtosecond laser pulses N2 - Indium islands on molybdenum coated glass can be grown in ordered arrays by surface structuring using a femtosecond laser. The effect of varying the molybdenum coated glass substrate temperature and the indium deposition rate on island areal density, volume and geometry is investigated and evaluated in a physical vapor deposition (PVD) process. The joined impact of growth conditions and spacing of the femtosecond laser structured spots on the arrangement and morphology of indium islands is demonstrated. The results yield a deeper understanding of the island growth and its precise adjustment to industrial requirements, which is indispensable for a technological application of such structures at a high throughput, for instance as precursors for the preparation of Cu(In,Ga)Se2 micro concentrator solar cells. KW - Indium islands KW - Femtosecond laser patterning KW - Diffusion KW - CIGSe micro solar cells PY - 2017 UR - http://www.sciencedirect.com/science/article/pii/S0169433216325764 DO - https://doi.org/10.1016/j.apsusc.2016.11.135 SN - 0169-4332 SN - 1873-5584 VL - 418 SP - 548 EP - 553 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-40551 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Moustapha, M.E. A1 - Farag, Z.R. A1 - Krüger, Simone A1 - Geesi, M.H. A1 - Friedrich, J.F. T1 - Potentiometric studies on the influence of poly(vinylpyrrolidone) on the thermal degradation behavior of poly(vinyl chloride) blends N2 - The thermal degradation behavior of poly(vinyl chloride) (PVC) and poly(N-vinylpyrrolidone) (PVP) blends was investigated using potentiometric measurements of the released HCl gas during the degradation process, estimating the degree of discoloration of the degraded samples and measuring the thermal stability values (Ts) values. The influence of the PVP percentage in the blend and, moreover, the addition of commercial dibasic lead carbonate stabilizer to the blend on its thermal stability was studied. It was found that the dehydrochlorination rate of the blend was promoted by increasing the PVP concentration in the blend. KW - Poly(vinyl chloride) KW - Poly(N-vinylpyrrolidone) KW - Potentiometry PY - 2019 DO - https://doi.org/10.3139/120.111304 SN - 0025-5300 VL - 61 IS - 2 SP - 179 EP - 183 PB - Carl Hanser CY - München AN - OPUS4-47360 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Tscheuschner, Georg A1 - Kaiser, Melanie N. A1 - Lisec, Jan A1 - Beslic, D. A1 - Muth, Thilo A1 - Krüger, M. A1 - Mages, H.-W. A1 - Dorner, B. G. A1 - Knospe, J. A1 - Schenk, J. A. A1 - Sellrie, F. A1 - Weller, Michael G. T1 - MALDI-TOF-MS-based identification of monoclonal murine anti-SARS-CoV-2 antibodies within one hour N2 - During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used thirty-five monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied onto the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 45 minutes and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 °C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0 (https://gets.shinyapps.io/ABID/). This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context. KW - SARS-CoV-2 antibody KW - Reproducibility crisis KW - Peptide mass fingerprinting KW - Monoclonal antibody KW - Traceability KW - Antibody identification KW - Identity KW - Antibody light chain KW - MALDI-TOF-MS KW - Trypsin KW - Acidic cleavage KW - Antibody subclass KW - Database KW - Peak overlap KW - ABID KW - Sulfuric acid KW - Online software KW - Sequencing KW - Peptide coverage PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-545182 DO - https://doi.org/10.20944/preprints202203.0229.v1 SN - 2310-287X SP - 1 EP - 24 PB - MDPI CY - Basel AN - OPUS4-54518 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -