TY - CONF A1 - Rurack, Knut T1 - Development of a Lab-on-a-Chip for the Detection of Nerve Agents with a Handheld Device N2 - The development of a Lab-on-a-Chip (LoC) is presented, which can detect reactive phosphorous compounds in the gas phase in combination with an optochemical hand-held sensor. The LoC prototype contains three pairs of sensing materials containing fluorescent indicator dyes in various carrier materials. By measuring the fluorescence response to phosphoryl chloride, a surrogate compound, the detection of chemical warfare agents (CWAs) in gas phase becomes possible within seconds, introducing a novel approach to CWA detection. T2 - IEEE Sensors Conference CY - Vienna, Austria DA - 29.10.2023 KW - Chemical warfare agents KW - Lab-on-a-chip KW - Handheld sensors KW - Toxic industrial chemicals KW - Fluorescence PY - 2023 AN - OPUS4-58815 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Rettig, W. A1 - Rurack, Knut A1 - Sczepan, M. ED - Valeur, B. ED - Brochon, J.-C. ED - O.S. Wolfbeis, T1 - From cyanines to styryl bases-photophysical properties, photochemical mechanisms, and cation sensing abilities of charged and neutral polymethinic dyes KW - Cyaninfarbstoffe KW - Styrylfarbstoffe KW - Photophysik KW - Photochemie KW - Kationen KW - Sensor PY - 2001 SN - 3-540-67779-8 SN - 1617-1306 N1 - Serientitel: Springer Series on Fluorescence – Series title: Springer Series on Fluorescence VL - 1 SP - 125 EP - 155 PB - Springer CY - Berlin AN - OPUS4-2086 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rurack, Knut T1 - Multifunctional hybrid microparticles for rapid cytometric and microfluidic mix-&-detect bioassays N2 - Besides the application-oriented parameters selectivity and sensitivity, simplicity and speed as well as robustness and reliability are the key features that determine success, dissemination and acceptance of analytical assays and methods for use in the field. For bioassays based on antibodies or nucleic acid binders, bead-based assays have become one of the main working horses of lab-based methods. However, these approaches are often limited by complicated and time-consuming workflows, which are mainly compensated by massive parallelisation, keeping the average cost and time-to-result within acceptable limits due to the sheer number and constant flow of samples processed. In on-site applications, where analyses need to be performed on suspicion or on demand because results are needed as quickly as possible for proactive decision making, the number, frequency and type of samples are much more diverse, so analytical assays need to be adapted to remain cost-effective and operable by non-expert users in environments where no lab infrastructure is available. With this in mind, we have developed a multifunctional hybrid microparticle platform that enables fast and simple mix-&-detect bioassays in combination with fluidic cytometry- and chip-based analytical methods. The present contribution will give an overview of the beads, their functions and how they can be combined with immunoanalytical and nucleic acid-based detection technologies. Starting from simple polymeric core/silica shell particles [1], we have progressively introduced coding [2,3], anti-fouling [2], high-surface area [4] and magnetic schemes [5] and in recent years have shown how these beads can be used in simple mix-&-detect assays for a range of different environmental, health and food analytes [1-5]. In addition to cytometry, we have also successfully implemented such assays using miniaturised microfluidic and strip-based approaches [6]. T2 - ANAKON 2025 CY - Leipzig, Germany DA - 10.03.2025 KW - Bead-based assay KW - Core-shell particles KW - Lab-on-a-chip KW - Mycotoxin PY - 2025 AN - OPUS4-62766 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Rurack, Knut A1 - Maus, M. T1 - Monitoring pH and solvent proticity with donor-acceptor-substituted biphenyls: a new approach towards highly sensitive and powerful fluorescent probes by tuning the molecular structure N2 - The ability and structural requirements of 4-dimethylamino-4-cyano-substituted biphenyls showing photoinduced intramolecular charge transfer (CT) to serve as hydrogen bond- or pH-sensitive fluorescent probes is investigated. The donor–acceptor (D–A) biphenyls I and II being planar in the CT excited state are most suitable as pH-sensitive fluorescent probes. The two compounds show analytically valuable features such as well-separated absorption and emission bands and signal changes spanning four orders of magnitude and can be employed in ratiometric, self-calibrating, and highly sensitive pH fluorosensing in the range 03.0.CO;2-3 SN - 1434-1948 SN - 1099-0682 VL - 2000 IS - 10 SP - 2271 EP - 2282 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-968 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rurack, Knut A1 - Al-Soufi, W. ED - Braslavsky, S. T1 - pH-Dependent Photophysics of 2,2'-Bipyridiyl-3-OL T2 - Workshop on Theoretical Organic (Photo) Chemistry ; 18th IUPAC Symposium on Photochemistry CY - Dresden, Germany DA - 2000-07-22 KW - pH KW - Photophysik KW - Bipyridyl OL PY - 2001 SN - 1010-6030 VL - 144 IS - 2-3 SP - 1 PB - Elsevier CY - Amsterdam AN - OPUS4-859 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Mayer-Enthart, Elke A1 - Sialelli, Julien A1 - Rurack, Knut A1 - Resch-Genger, Ute A1 - Köster, D. A1 - Seitz, H. T1 - Toward Improved Biochips Based on Rolling Circle Amplification - Influences of the Microenvironment on the Fluorescence Properties of Labeled DNA Oligonucleotides KW - Rolling circle amplification KW - Fluorescence KW - Biochips KW - Microarrays KW - Signal amplification KW - Cy3-labeled oligonucleotides KW - DNA technology PY - 2008 DO - https://doi.org/10.1196/annals.1430.022 SN - 0077-8923 SN - 1749-6632 SN - 0094-8500 VL - 1130 SP - 287 EP - 292 PB - New York Academy of Sciences CY - New York, NY AN - OPUS4-17633 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Rurack, Knut A1 - Schulz, Burkhard A1 - Reck, Günter A1 - Resch-Genger, Ute A1 - Bricks, J. A1 - Maus, M. T1 - Substituted 1,5-Diphenyl-3-benzothiazol-2-yl-Delta2-pyrazolines - Synthesis, X-ray Structure, Photophysics, and Cation Complexation Properties N2 - The spectroscopic properties of 1-phenyl-3-benzothiazol-2-yl-5-(4-R-phenyl)-2-pyrazolines are strongly dependent on both the electronic nature of the substituent R and solvent polarity. As revealed by spectroscopic studies as a function of solvent polarity as well as temperature, for electron-rich amino donor substituents in polar solvents, deactivation of the strongly emissive charge transfer (CT) state of the basic 1-phenyl-3-benzothiazol-2-yl-2-pyrazoline chromophore has to compete with a fast intramolecular electron transfer (ET) quenching reaction. In the case of the dimethylamino derivative (R = DMA), the rate constant of ET in acetonitrile was determined to ket = 3 × 1010 s-1. This ET process can be utilized for metal ion sensing by introducing nitrogen containing aza crown ether receptor units to the 4-position of the 5-phenyl group. The spectroscopically determined ET rates of the 5-(N-alkyl)anilino substituents, a DMA, a tetrathia- (AT415C5), and a tetraoxa-monoaza-15-crown-5 (A15C5) group, correlate with electrochemical data and increase in the order AT415C5 < A15C5 < DMA. The metal ion sensing abilities of the two crowned derivatives are presented, and the different signaling mechanisms include binding to the crown ether in the 4-R-position, chelate formation in the 3-benzothiazol-2-yl-2-pyrazoline moiety, and electrophotochemical detection. Furthermore, the rigid "pseudo spiro" geometry of the molecules, which holds the three substituents of the central 2-pyrazoline ring in a fixed prearrangement, was confirmed by X-ray structure analysis. KW - Farbstoff KW - Fluoreszenz KW - Kationen KW - Photophysik PY - 2000 DO - https://doi.org/10.1021/jp993404q SN - 1089-5639 SN - 1520-5215 VL - 104 IS - 26 SP - 6171 EP - 6188 PB - Soc. CY - Washington, DC AN - OPUS4-841 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Rurack, Knut A1 - Bricks, J.L. A1 - Slominskii, J.L. A1 - Resch-Genger, Ute T1 - Cation complexing fluorescence probes containing the benz[c,d]indole fluorophore PY - 1998 SN - 0143-7208 SN - 1873-3743 VL - 36 IS - 2 SP - 121 EP - 138 PB - Elsevier Science CY - Kidlington AN - OPUS4-2098 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rurack, Knut A1 - Unger, Brita A1 - Müller, Ralf A1 - Resch-Genger, Ute T1 - SiO2-Gele als Matrices für Fluoreszenzmarker T2 - 75. Glastechnische Tagung CY - Wernigerode, Deutschland DA - 2001-05-21 PY - 2001 SP - 8 EP - 11 PB - DGG, Deutsche Glastechnische Gesellschaft e.V. CY - Frankfurt/M. AN - OPUS4-2084 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Resch-Genger, Ute A1 - Rurack, Knut T1 - Determination of the photoluminescence quantum yield of dilute dye solutions (IUPAC technical report) N2 - Procedures for the determination of photoluminescence quantum yields with optical methods are addressed, and challenges associated with these measurements are discussed. Special emphasis is dedicated to relative measurements of fluorescent (i.e., short excited-state lifetime), transparent, dilute dye solutions in conventional cuvettes in a 0°/90° measurement geometry. Recommendations on the selection of suitable quantum yield standards are presented, and requirements for the documentation of photoluminescence quantum yields are derived. KW - Absorption KW - Dilute dye solutions KW - Fluorescence KW - IUPAC Analytical Chemistry Division KW - IUPAC Organic and Biomolecular Chemistry Division KW - IUPAC Physical and Biophysical chemistry division KW - Luminescence KW - Photoluminescence quantum yields PY - 2013 DO - https://doi.org/10.1351/PAC-REP-12-03-03 SN - 0033-4545 SN - 1365-3075 VL - 85 IS - 10 SP - 2005 EP - 2026 PB - Union CY - Research Triangle Park, NC AN - OPUS4-29323 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Rurack, Knut A1 - Resch-Genger, Ute T1 - Design, Synthese und Charakterisierung von Fluoreszenzstandards und Fluoreszenzmarkern für die zeitaufgelöste Fluorometrie N2 - Im Rahmen dieses Projekts wurden Fluoreszenzfarbstoffe und neue intrinsische und konjugierte Fluoreszenzmarker mit Sauerstoff, Schwefel, Stickstoff-Heteromakrozyklen als kationenspezifische Rezeptoren für analytische Applikationen der zeitaufgelösten Fluorometrie von der Kiewer Gruppe synthetisiert. Die in Berlin an diesen Verbindungen durchgeführten fluorometrischen Charakterisierungen und spektroskopischen Untersuchungen zum Kationenkomplexierungsverfahren ermöglichten die Aufstellung von ersten Designprinzipien für Fluoreszenzmarker für Übergangs- und Schwermetallionen. T3 - BAM Forschungsberichtreihe - 223 PY - 1998 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-2273 SN - 978-3-89701-038-0 SN - 0938-5533 VL - 223 SP - 1 EP - 40 PB - Wirtschaftsverlag NW CY - Bremerhaven AN - OPUS4-227 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Resch-Genger, Ute A1 - Rurack, Knut ED - Lieberman, R. T1 - Steady-state and time-resolved fluorometry of fluorescent pollutants and heavy metal complexes N2 - Time-resolved laser-induced fluorescence spectroscopy is one of the most sensitive optical methods which is well suited for on-line in situ analysis. Here, three examples for the steady- state and time-resolved fluorescence analysis of environmentally important analytes, the fluorescent monoaromatic hydrocarbons benzene, toluene, and xylene as well as non fluorescent heavy metal ions forming a fluorescent complex with a cation coordinating fluorescence probe, are presented and the potential of both methods is discussed. For BTX, various mixtures of the spectrally similar compounds B, T, and X showing different fluorescence lifetimes were studied with both methods. As an example for fluorometric metal ion analysis, the fluorescence probe BP(OH)2 (2,2'-bipyridyl- 3,3'-diol) was employed for the determination of d10 metal ions in water and the newly developed fluorescence probe APTA for the detection of Cu(II). Cation complexation of BP(OH2 yields spectrally very similar complexes which differ in their fluorescence lifetimes. Complexation of APTA to Cu(II) leads to small spectral changes and a strong increase in fluorescence quantum yield and lifetime. For the analytes studied, a comparison of the detection limits, standard deviations, and linear dynamic range of both methods clearly demonstrates the analytical potential of time-resolved fluorometry. T2 - Conference CY - Munich, Germany DA - 1997-06-16 KW - Fluorescence KW - Time-resolved KW - Probe KW - Heavy metal ion KW - Cation complex PY - 1997 SN - 0-8194-2526-5 DO - https://doi.org/10.1117/12.276139 SN - 1605-7422 N1 - Serientitel: SPIE proceedings series – Series title: SPIE proceedings series IS - 3105 SP - 96 EP - 103 PB - SPIE CY - Bellingham, Wash. AN - OPUS4-2099 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Unger, Brita A1 - Rurack, Knut A1 - Müller, Ralf A1 - Jancke, Harald A1 - Resch-Genger, Ute T1 - Microscopic vs. macroscopic structural evolution of SiO2 sols and gels employing a tailor-made fluorescent reporter dye N2 - The short- and long-term structural evolution and aging of sol–gel materials prepared under mild conditions are studied by fluorometry, dynamic light scattering (DLS), 29Si NMR and gravimetry. In combination with the first, a newly developed donor–acceptor-substituted fluorescent probe with two acidophilic sites of different strength and topology is introduced. Its performance in detecting changes in parameters such as polarity, proticity, and pore volume on the microscopic scale is compared to two other dyes and to the macroscopic changes revealed by DLS, NMR and gravimetry. At early stages of the sol–gel process, fluorometry, DLS and NMR reveal similar changes which are typical of the structural evolution of SiO2 sols having different synthesis parameters. During later times of sol aging, DLS and NMR reflect the growth of the network and indicate that all sols approach similar structural equilibria. Fluorometry supports these findings by signalling a gradual decrease in mobile species with high proton activity. The formation of the spanning cluster upon gelation is strongly evident only from DLS results while the local environment of the fluorophore remains largely unchanged. However, the fluorescent dye is able to report microscopic changes even during long-term aging. Drying of the gels with a pronounced contraction of solvated pores is readily observable by fluorometry and gravimetry. During the late reaction stages, the fluorescence properties of the tailor-made dye are still sensitive toward the environment. PY - 2005 DO - https://doi.org/10.1039/b501622c SN - 0959-9428 SN - 1364-5501 VL - 15 IS - 30 SP - 3069 EP - 3083 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-10378 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Krahl, T. A1 - Beer, F. A1 - Relling, A. A1 - Gawlitza, Kornelia A1 - Rurack, Knut A1 - Kemnitz, E. T1 - Toward Luminescent Composites by Phase Transfer of SrF2 :Eu3+ Nanoparticles Capped with Hydrophobic Antenna Ligands N2 - Transparent dispersions of hydrophobic SrF2 :Eu3+ nanoparticles in cyclohexane with up to 20% europium were obtained by fluorolytic sol-gel synthesis followed by Phase transfer into cyclohexane through capping with sodium dodecylbenzenesulfonate (SDBS). The particles were characterized by TEM, XRD and DLS as spherical objects with a diameter between 6 and 11 nm in dry state. 1H-13CP MAS NMR experiments revealed the binding of the anionic sulfonate head group to the particle surface. The particles show bright red luminescence upon excitation of the aromatic capping agents, acting as antennas for an Energy transfer from the benzenesulfonate unit to the Eu3+ centers in the particles. This synthesis method overcomes the current obstacle of the fluorolytic sol-gel synthesis that transparent dispersions can be obtained directly only in hydrophilic solvents. To demonstrate the potential of such hydrophobized alkaline-earth fluoride particles, transparent luminescent organic-inorganic composites with 10% SrF2 :Eu3+ embedded into polyTEGDMA, polyBMA, poly-BDDMA and polyD3MA, respectively, were prepared, endowing the polymers with the luminescence features of the nanoparticles. KW - Nanoparticles KW - Fluorides KW - Sol-gel process KW - Organic-inorganic hybrid composites PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-508668 DO - https://doi.org/10.1002/cnma.202000058 SP - 1 EP - 11 PB - Wiley AN - OPUS4-50866 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Hecht, Mandy A1 - Witthuhn, Heike A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Mix‐&‐read determination of Mercury(II) at trace levels with hybrid mesoporous silica materials incorporating fluorescent probes by a simple mix‐&‐load technique N2 - The synthesis, characterization, and application of mesoporous materials containing boron–dipyrromethene (BODIPY) moieties that allow the sensitive and selective detection of HgII in aqueous environments by fluorescence enhancement is reported. For this purpose, BODIPY dye I containing a thia‐aza crown ether receptor as the fluorescent probe for the detection of HgII in aqueous environments is encapsulated into mesoporous materials to avoid self‐quenching or aggregation in water. Determination of HgII is accomplished within a few seconds with high selectivity and sensitivity, reaching a limit of detection of 12 ppt. The determination of trace amounts of HgII in natural waters and in fish extracts is demonstrated by using our sensing material. The incorporation of the material into several μ‐PAD strips yields a portable, cheap, quick, and easy‐to‐handle tool for trace HgII analysis in water. KW - Dyes/pigments KW - Test strips KW - Mesoporous materials KW - Mercury KW - Fluorescence PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-460138 DO - https://doi.org/10.1002/open.201800277 SN - 2191-1363 VL - 7 IS - 12 SP - 957 EP - 968 PB - Wiley-VCH CY - Weinheim AN - OPUS4-46013 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Alnajjar, M. A. A1 - Bartelmeß, Jürgen A1 - Hein, R. A1 - Ashokkumar, Pichandi A1 - Nilam, M. A1 - Nau, W. M. A1 - Rurack, Knut A1 - Hennig, A. T1 - Rational design of boron-dipyrromethene (BODIPY) reporter dyes for cucurbit[7]uril N2 - We introduce herein boron-dipyrromethene (BODIPY) dyes as a new class of fluorophores for the design of reporter dyes for supramolecular host–guest complex formation with cucurbit[7]uril (CB7). The BODIPYs contain a protonatable aniline nitrogen in the meso-position of the BODIPY chromophore, which was functionalized with known binding motifs for CB7. The unprotonated dyes show low fluorescence due to photoinduced electron transfer (PET), whereas the protonated dyes are highly fluorescent. Encapsulation of the binding motif inside CB7 positions the aniline nitrogen at the carbonyl rim of CB7, which affects the pKa value, and leads to a host-induced protonation and thus to a fluorescence increase. The possibility to tune binding affinities and pKa values is demonstrated and it is shown that, in combination with the beneficial photophysical properties of BODIPYs, several new applications of host–dye reporter pairs can be implemented. This includes indicator displacement assays with favourable absorption and emission wavelengths in the visible spectral region, fluorescence correlation spectroscopy, and noncovalent surface functionalization with fluorophores. KW - BODIPY KW - Cucurbituril KW - Fluorescence KW - PH KW - Photoinduced Electron Transfer KW - Supramolecular Chemistry PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-456361 UR - https://www.beilstein-journals.org/bjoc/content/pdf/1860-5397-14-171.pdf DO - https://doi.org/10.3762/bjoc.14.171 SN - 1860-5397 VL - 14 SP - 1961 EP - 1971 PB - Beilstein-Institut CY - Frankfurt a. M. AN - OPUS4-45636 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gotor, Raul A1 - Ashokkumar, Pichandi A1 - Hecht, Mandy A1 - Keil, Karin A1 - Rurack, Knut T1 - Optical pH Sensor Covering the Range from pH 0−14 Compatible with Mobile-Device Readout and Based on a Set of Rationally Designed Indicator Dyes N2 - In this work, a family of pH-responsive fluorescent probes has been designed in a rational manner with the aid of quantum chemistry tools, covering the entire pH range from 0−14. Relying on the boron−dipyrromethene (BODIPY) core, all the probes as well as selected reference dyes display very similar spectroscopic properties with ON−OFF fluorescence switching responses, facilitating optical readout in simple devices used for detection and analysis. Embedding of the probes and reference dyes into hydrogel spots on a plastic strip yielded a test strip that reversibly indicates pH with a considerably small uncertainty of ~0.1 pH units. These strips are not only reusable but, combined with a 3D-printed case that can be attached to a smartphone, the USB port of which drives the integrated LED used for excitation, allows for autonomous operation in on-site or in-the-field applications; the developed Android application software (“app”) further simplifies operation for unskilled users. KW - Fluorescence KW - pH measurement KW - BODIPY KW - Smartphone KW - Test strip PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-422571 DO - https://doi.org/10.1021/acs.analchem.7b01903 SN - 0003-2700 SN - 1520-6882 VL - 89 IS - 16 SP - 8437 EP - 8444 PB - American Chemical Society CY - Washington, D.C., USA AN - OPUS4-42257 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bell, Jérémy A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Die 3D-Mikrofluidik mit molekular geprägten Polymerpartikeln eröffnet neue Möglichkeiten der selektiven Pestizid-Bestimmung in Wasser N2 - 2,4-D ist ein in der Landwirtschaft weitverbreitetes Pflanzenschutzmittel, das Grundwasser kontaminiert, sich innerhalb der Nahrungskette anreichert und Umwelt- und Gesundheitsprobleme verursachen kann. Hier stellen die Autoren ein mikrofluidisches Nachweissystem für die Echtzeitdetektion von 2,4-D in Grund- oder Oberflächenwasser vor. Es basiert auf der Kombination 2,4-D-selektiver, fluoreszierender, molekular geprägter Polymer-(MIP-)Mikropartikel mit einem 3D-mikrofluidischen Extraktions- und Detektionssystem. Messungen vor Ort sollen damit künftig möglich sein. KW - 3D-Mikrofluidik KW - Sensorpartikel KW - MIP KW - Pestizid PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-444781 UR - https://www.labo.de/epaper/LA0318/index.html SN - 0344-5208 IS - 3 SP - 10 EP - 13 PB - WEKA Business Medien GmbH AN - OPUS4-44478 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bell, Jérémy A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Mit Tröpfchen Spielen N2 - Mikrofluidische Systeme sind leistungsstarke analytische Tools mit attraktiven Eigenschaften wie miniaturisierter Größe, geringem Reagenzien und Probenverbrauch, schneller Ansprech- und kurzer Messzeit. Der Bedarf solcher leistungsstarken, miniaturisierten und direkt vor Ort anwendbaren Sensorsysteme steigt kontinuierlich, hauptsächlich durch das Bedürfnis der Gesellschaft, schneller, besser und umfassender über kritische Faktoren im Lebens- und Arbeitsumfeld sowie der Umwelt informiert zu sein. KW - Mikrofluidik KW - Sensorpartikel PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-459120 UR - https://www.git-labor.de/forschung/umwelt/mit-troepfchen-spielen VL - 8 IS - 8 SP - 2 EP - 4 PB - Wiley-VCH Verlag GmbH & Co. KGaA CY - Weinheim, Deutschland AN - OPUS4-45912 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bell, Jérémy A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Playing with Droplets N2 - Microfluidic devices are powerful analytical tools with appealing features such as miniaturized size, low reagent and sample consumption, rapid response and short measurement times. As society wants to be ever better, earlier and more comprehensively informed about critical factors in life, work, and the environment, the demand for powerful measurement devices for use outside of the laboratory constantly increases. KW - Microfluidics KW - Sensory particles PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-459131 UR - https://www.laboratory-journal.com/ VL - 4 SP - 2 EP - 4 PB - Wiley-VCH Verlag GmbH & Co. KGaA CY - Weinheim, Deutschland AN - OPUS4-45913 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Hecht, Mandy A1 - Witthuhn, Heike A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Cover profile for the article "Mix‐&‐read determination of Mercury(II) at trace levels with hybrid mesoporous silica materials incorporating fluorescent probes by a simple mix‐&‐load technique" N2 - Invited for this month’s cover picture is the group of Dr. Knut Rurack at the Department of Analytical Chemistry; Reference Materials at the Bundesanstalt fuer Materialforschung und -pruefung (BAM) in Berlin (Germany). The cover picture shows how differences in color and fluorescence on a test strip can be easily read out with a mobile device. Two reference spots Frame the sensitive spot that indicates the presence of trace amounts of HgII below the threshold in a natural water sample. This dipstick contains a hybrid material that combines boron-dipyrromethene (BODIPY) probes sterically loaded into specifically tailored mesoporous silica particles, allowing for ultrasensitive HgII detection through enhanced fluorescence in a few seconds. The applicability in real water samples and fish extracts are also studied. KW - Mercury KW - Fluorescence KW - Dip-stick assay KW - Group profile PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-471267 DO - https://doi.org/10.1002/open.201800231 SN - 2191-1363 VL - 7 IS - 12 SP - 932 EP - 933 PB - Wiley-VCH CY - Weinheim AN - OPUS4-47126 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Rurack, Knut T1 - Streifenschnelltest mit ppt-Empfindlichkeit durch Kombination von Elektrochemilumineszenz-Detektion mit Aptamer-gesteuerter Indikatorfreisetzung aus mesoporösen Nanopartikeln N2 - Die Kombination aus elektrogenerierter Chemilumineszenz (ECL) und Aptamer-gesteuerter Indikatorfreisetzung (gAID) aus magnetischen mesoporösen Silica-Nanopartikeln, die in mit Poly(ethylenglycol) und N-(3-Triethoxysilylpropyl) diethanolamin funktionalisiertes Glasfaserpapier eingebettet sind, ermöglichte die Entwicklung eines Schnelltests, mit dem sich Penicillin direkt in verdünnter Milch bis auf 50 +/- 9 ppt in <5 min nachweisen lässt. Die kovalente Bindung der Aptamer-Kappe an das Siliciumdioxid-Gerüst ermöglichte den Verschluss der Poren durch nicht-kovalente elektrostatische Wechselwirkungen mit Oberflächen-Aminogruppen. Die Bindung von Penicillin führte zu einer Faltung des Aptamers, wodurch der zuvor in das Material geladene ECL-Reporter Ru(bpy)32+ freigesetzt wurde und nach lateralem Fluss von einer Smartphone-Kamera nach elektrochemischer Anregung mit einer in einen 3D-gedruckten Halter eingesetzten Siebdruckelektrode detektiert wird. Der Ansatz ist einfach, generisch und bietet Vorteile hinsichtlich Empfindlichkeit, Messunsicherheit und Robustheit gegenüber konventioneller Fluoreszenz- oder elektrochemischer Detektion, insbesondere für Point-of-Need-Analysen anspruchsvoller Matrices und Analyten im Ultraspurenbereich. KW - Schnelltests KW - Elektrochemilumineszenz KW - Aptamere KW - Teststreifen KW - Gesteuerte Freisetzung PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-540205 DO - https://doi.org/10.1002/ange.202110744 VL - 133 IS - 50 SP - 26491 EP - 26501 PB - Wiley-VCH CY - Weinheim AN - OPUS4-54020 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -