TY - JOUR A1 - Hoffmann, Katrin A1 - Resch-Genger, Ute A1 - Nitschke, R. T1 - Simple Tool for the Standardization of Confocal Spectral Imaging Systems JF - GIT Imaging & Microscopy KW - Standards KW - Fluorescence reference material KW - Quality assurance KW - Fluorescence microscopy PY - 2005 SN - 1439-4243 SN - 1863-7809 VL - 7 IS - 3 SP - 18 EP - 19 PB - GIT-Verl. CY - Darmstadt AN - OPUS4-11070 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Hoffmann, Katrin A1 - Resch-Genger, Ute A1 - Nitschke, R. ED - O.S. Wolfbeis, ED - Resch-Genger, Ute T1 - Comparability of fluorescence microscopy data and need for instrument characterization of spectral scanning microscopes T2 - Standardization and quality assurance in fluorescence measurements II - Bioanalytical and biomedical applications N2 - The aim of this article is to illustrate the need for an improved quality assurance in fluorescence microscopy. From the instrument-side, this can be achieved by a better understanding, consideration, and regular control of the instrument-specific parameters and quantities affecting measured fluorescence signals. Particularly, the need for requirements on physical- and chemical-type instrument standards for the characterization and performance validation of spectral fluorescence microscopes (SFMs) is discussed and suitable systems are presented. Special emphasis is given to spectral fluorescence standards and to day-to-day intensity standards for SFMs. Fluorescence standards and well-characterized fluorescence microscopes are the first and essential steps towards the comparability and the understanding of the variability in fluorescence microscopy data in medical and life sciences. In addition, standards enable the distinction between instrument-specific variations and fluorescent label- or probe-related uncertainties as well as generally sample-related effects. KW - Fluorescence spectroscopy KW - Fluorescence microscopy KW - Quality assurance KW - Calibration KW - Standard KW - Comparability KW - Quantification PY - 2008 SN - 978-3-540-70570-3 DO - https://doi.org/10.1007/4243_2008_028 SN - 1617-1306 N1 - Serientitel: Springer Series on Fluorescence – Series title: Springer Series on Fluorescence VL - 6 IS - Part A SP - 89 EP - 116 PB - Springer CY - Berlin, Heidelberg AN - OPUS4-18995 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -