TY - JOUR A1 - Schäfer, A. A1 - Konrad, R. A1 - Kuhnigk, T. A1 - Kämpfer, P. A1 - Hertel, Horst A1 - König, H. T1 - Hemicellulose-degrading bacteria and yeasts from the termite gut JF - Journal of applied bacteriology PY - 1996 SN - 0021-8847 VL - 80 SP - 471 EP - 478 PB - Blackwell CY - Oxford AN - OPUS4-11826 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Fröhlich, J. A1 - Koustiane, C. A1 - Kämpfer, P. A1 - Rosselló-Mora, R. A1 - Valens, M. A1 - Berchtold, M. A1 - Kuhnigk, T. A1 - Hertel, Horst A1 - Maheshwari, D.K. A1 - König, H. T1 - Occurrence of rhizobia in the gut of the higher termite Nasutitermes nigriceps JF - Systematic and applied microbiology N2 - Wood-eating termites feed on a diet highly deficient in nitrogen. They must complement their diet with the aid of nitrogen-fixing bacteria. Nitrogen fixation in the gut has been demonstrated, but information about nitrogen-fixing bacteria in pure culture is scarce. From the higher termite Nasutitermes nigriceps the symbiotic bacterial strain M3A was isolated, which thrives in the hindgut contents. The Gram-negative strain exhibited similarities to the species of the genus Ensifer (including Sinorhizobium) on the basis of morphological and physiological/biochemical features. The 16S rRNA gene analysis showed the highest sequence similarity of the isolate M3A to Ensifer adhaerens (>99%; ATCC 33499). The DNA–DNA hybridization revealed a similarity of 66% with E. adhaerens (NCIMB12342T). In contrast to the type strain the isolate M3A possesses the capacity to nodulate plant roots. This is the first report on the detailed identification of a rhizobia-related strain from the intestinal tract of animals. Strain M3A has been deposited with two culture collections (DSM10169; ATCC BAA-396). KW - Rhizobia KW - Ensifer KW - Termites KW - Intestinal flora KW - Nitrogen fixation PY - 2007 SN - 0723-2020 VL - 30 IS - 1 SP - 68 EP - 74 PB - Elsevier CY - Amsterdam AN - OPUS4-14541 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -