TY - JOUR A1 - Diehn, S. A1 - Zimmermann, B. A1 - Tafintseva, V. A1 - Seifert, S. A1 - Bagcioglu, M. A1 - Ohlson, M. A1 - Weidner, Steffen A1 - Fjellheim, S. A1 - Kohler, A. A1 - Kneipp, Janina T1 - Combining Chemical Information From Grass Pollen in Multimodal Characterization N2 - The analysis of pollen chemical composition is important to many fields, including agriculture, plant physiology, ecology, allergology, and climate studies. Here, the potential of a combination of different spectroscopic and spectrometric methods regarding the characterization of small biochemical differences between pollen samples was evaluated using multivariate statistical approaches. Pollen samples, collected from three populations of the grass Poa alpina, were analyzed using Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, surface enhanced Raman scattering (SERS), and matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). The variation in the sample set can be described in a hierarchical framework comprising three populations of the same grass species and four different growth conditions of the parent plants for each of the populations. Therefore, the data set can work here as a model system to evaluate the classification and characterization ability of the different spectroscopic and spectrometric methods. ANOVA Simultaneous Component Analysis (ASCA) was applied to achieve a separation of different sources of variance in the complex sample set. Since the chosen methods and sample preparations probe different parts and/or molecular constituents of the pollen grains, complementary information about the chemical composition of the pollen can be obtained. By using consensus principal component analysis (CPCA), data from the different methods are linked together. This enables an investigation of the underlying global information, since complementary chemical data are combined. The molecular information from four spectroscopies was combined with phenotypical information gathered from the parent plants, thereby helping to potentially link pollen chemistry to other biotic and abiotic parameters. KW - Pollen KW - MALDI-TOF MS KW - FTIR KW - Raman KW - Multivariate analyses PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-504822 DO - https://doi.org/10.3389/fpls.2019.01788 VL - 10 SP - 1788 AN - OPUS4-50482 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Zerbst, Uwe A1 - Beretta, S. A1 - Köhler, G. A1 - Lawton, A. A1 - Vormwald, M. A1 - Beier, H.T. A1 - Klinger, Christian A1 - Cerný, I. A1 - Rudlin, J. A1 - Heckel, Thomas A1 - Klingbeil, Dietmar T1 - Safe life and damage tolerance aspects of railway axles - A review N2 - The paper gives an overview on safe life and damage tolerance methods applied to railway axles. It describes failure scenarios due to fatigue crack initiation and propagation. Besides common aspects of design, specific features such as corrosion and impact damage from flying ballast are discussed which may reduce the fatigue strength of axles during service. Potential effects of non-metallic inclusions from the steel manufacturing process are addressed in the context of the very high number of loading cycles railway axles are designed for. With respect to damage tolerance general lines of fracture mechanics residual lifetime analyses are introduced. More specific discussion is provided on aspects such as the threshold value of fatigue crack propagation and reliability aspects of non-destructive inspection. KW - Railway axle KW - Safe life design KW - Damage tolerance KW - Fatigue strength KW - Fatigue crack propagation KW - Inspection intervals PY - 2013 DO - https://doi.org/10.1016/j.engfracmech.2012.09.029 SN - 0013-7944 SN - 1873-7315 VL - 98 SP - 214 EP - 271 PB - Elsevier Science CY - Kidlington AN - OPUS4-27779 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Heckmann, A. A1 - Dümmler, S. A1 - Pauli, Jutta A1 - Margraf, M. A1 - Köhler, J. A1 - Stich, D. A1 - Lambert, C. A1 - Fischer, I. A1 - Resch-Genger, Ute T1 - Highly fluorescent open-shell NIR dyes: the time-dependence of back electron transfer in triarylamine-perchlorotriphenylmethyl radicals KW - Triarylamine-perchlorotriphenylmethyl radical KW - Dono-acceptor compounds KW - CT state KW - Fluorescence quantum KW - Yield KW - Life time PY - 2009 DO - https://doi.org/10.1021/jp908425w SN - 1932-7447 SN - 1089-5639 VL - 113 SP - 20958 EP - 20966 PB - Soc. CY - Washington, DC AN - OPUS4-20757 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Strassert, J.F.H. A1 - Köhler, T. A1 - Wienemann, T. H. G. A1 - Ikeda-Ohtsubo, W. A1 - Faivre, N. A1 - Franckenberg, S. A1 - Plarre, Rüdiger A1 - Radek, R. A1 - Brune, A. T1 - 'Candidatus ancillula trichonymphae', a novel lineage of endosymbiotic actinobacteria in termite gut flagellates of the genus trichonympha N2 - Termite gut flagellates are colonized by host-specific lineages of ectosymbiotic and endosymbiotic bacteria. Previous studies have shown that flagellates of the genus Trichonympha may harbour more than one type of symbiont. Using a comprehensive approach that combined cloning of SSU rRNA genes with fluorescence in situ hybridization and electron microscopy, we investigated the phylogeny and subcellular locations of the symbionts in a variety of Trichonympha species from different termites. The flagellates in Trichonympha Cluster I were the only species associated with 'Endomicrobia', which were located in the posterior part of the cell, confirming previous results. Trichonympha species of Cluster II from the termite genus Incisitermes (family Kalotermitidae) lacked 'Endomicrobia' and were associated with endosymbiotic Actinobacteria, which is highly unusual. The endosymbionts, for which we suggest the name 'Candidatus Ancillula trichonymphae', represent a novel, deep-branching lineage in the Micrococcineae that consists exclusively of clones from termite guts. They preferentially colonized the anterior part of the flagellate host and were highly abundant in all species of Trichonympha Cluster II except Trichonympha globulosa. Here, they were outnumbered by a Desulfovibrio species associated with the cytoplasmic lamellae at the anterior cell pole. Such symbionts are present in both Trichonympha clusters, but not in all species. Unlike the intracellular location reported for the Desulfovibrio symbionts of Trichonympha agilis (Cluster I), the Desulfovibrio symbionts of T. globulosa (Cluster II) were situated in deep invaginations of the plasma membrane that were clearly connected to the exterior of the host cell. PY - 2012 DO - https://doi.org/10.1111/1462-2920.12012 SN - 1462-2912 SN - 1462-2920 VL - 14 IS - 12 SP - 3259 EP - 3270 PB - Blackwell Science CY - Oxford AN - OPUS4-27566 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Diehn, Sabrina A1 - Zimmermann, B. A1 - Bagcioglu, M. A1 - Seifert, Stephan A1 - Kohler, A. A1 - Ohlson, M. A1 - Fjellheim, S. A1 - Weidner, Steffen A1 - Kneipp, Janina T1 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaption of grass pollen composition N2 - MALDI time-of-flight mass spectrometry (MALDI-TOF MS) has become a widely used tool for the classification of biological samples. The complex chemical composition of pollen grains leads to highly specific, fingerprint-like mass spectra, with respect to the pollen species. Beyond the species-specific composition, the variances in pollen chemistry can be hierarchically structured, including the level of different populations, of environmental conditions or different genotypes. We demonstrate here the sensitivity of MALDI-TOF MS regarding the adaption of the chemical composition of three Poaceae (grass) pollen for different populations of parent plants by analyzing the mass spectra with partial least squares discriminant analysis (PLS-DA) and principal component analysis (PCA). Thereby, variances in species, population and specific growth conditions of the plants were observed simultaneously. In particular, the chemical pattern revealed by the MALDI spectra enabled discrimination of the different populations of one species. Specifically, the role of environmental changes and their effect on the pollen chemistry of three different grass species is discussed. Analysis of the Group formation within the respective populations showed a varying influence of plant genotype on the classification, depending on the species, and permits conclusions regarding the respective rigidity or plasticity towards environmental changes. KW - Pollen KW - MALDI-TOF MS KW - Classification KW - Partial least square discriminant analysis (PLS-DA) KW - Principal component analysis (PCA) PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-465294 DO - https://doi.org/10.1038/s41598-018-34800-1 SN - 2045-2322 VL - 8 IS - 1 SP - 16591, 1 EP - 11 PB - Nature AN - OPUS4-46529 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Protasov, E A1 - Noah, J O A1 - Kästle Silva, J O A1 - Mies, U S A1 - Hervé, V A1 - Dietrich, C A1 - Lang, K A1 - Mikulski, L A1 - Platt, K A1 - Poehlein, A A1 - Köhler-Ramm, T A1 - Miambi, E A1 - Boga, H I A1 - Feldewert, C A1 - Ngugi, G K A1 - Plarre, Rüdiger A1 - Sillam-Dussès, D A1 - Šobotník, J A1 - Daniel, R A1 - Brune, A T1 - Diversity and taxonomic revision of methanogens and other archaea in the intestinal tract of terrestrial arthropods N2 - Methane emission by terrestrial invertebrates is restricted to millipedes, termites, cockroaches, and scarab beetles. The arthropod-associated archaea known to date belong to the orders Methanobacteriales, Methanomassiliicoccales, Methanomicrobiales, and Methanosarcinales, and in a few cases also to nonmethanogenic Nitrososphaerales and Bathyarchaeales. However, all major host groups are severely undersampled, and the taxonomy of existing lineages is not well developed. Full-length 16S rRNA gene sequences and genomes of arthropod-associated archaea are scarce, reference databases lack resolution, and the names of many taxa are either not validly published or under-classified and require revision. Here, we investigated the diversity of archaea in a wide range of methane-emitting arthropods, combining phylogenomic analysis of isolates and metagenome-assembled genomes (MAGs) with amplicon sequencing of full-length 16S rRNA genes. Our results allowed us to describe numerous new species in hitherto undescribed taxa among the orders Methanobacteriales (Methanacia, Methanarmilla, Methanobaculum, Methanobinarius, Methanocatella, Methanoflexus, Methanorudis, and Methanovirga, all gen. nova), Methanomicrobiales (Methanofilum and Methanorbis, both gen. nova), Methanosarcinales (Methanofrustulum and Methanolapillus, both gen. nova), Methanomassiliicoccales (Methanomethylophilaceae fam. nov., Methanarcanum, Methanogranum, Methanomethylophilus, Methanomicula, Methanoplasma, Methanoprimaticola, all gen. nova), and the new family Bathycorpusculaceae (Bathycorpusculum gen. nov.). Reclassification of amplicon libraries from this and previous studies using this new taxonomic framework revealed that arthropods harbor only CO2 and methyl-reducing hydrogenotrophic methanogens. Numerous genus-level lineages appear to be present exclusively in arthropods,suggesting long evolutionary trajectories with their termite, cockroach, and millipede hosts, and a radiation into various microhabitats and ecological nichesprovided by their digestive tracts (e.g., hindgut compartments, gut wall, or anaerobic protists). The distribution patterns among the different host groups are often complex, indicating a mixed mode of transmission and a parallel evolution of invertebrate and vertebrate-associated lineages. KW - Nitrososphaerales KW - Archaea KW - Methanogens KW - Gut microbiota KW - Termites KW - Cockroaches KW - Millipedes KW - Bathyarchaeia PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-588230 DO - https://doi.org/10.3389/fmicb.2023.1281628 SN - 1664-302X VL - 14 SP - 1 EP - 21 PB - Frontiers AN - OPUS4-58823 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -