TY - GEN A1 - Pötsche, P. A1 - Bhattacharyya, A. R. A1 - Janke, A. A1 - Goering, Harald T1 - Conductive polymers by filling polymer melts with carbon nanotubes T2 - Nanofair 2003 T2 - 2. Tagung Nanofair 2003 CY - Dresden, Germany DA - 2003-11-20 KW - Nanocomposites KW - Melt mixing KW - Condutivity PY - 2003 SN - 3-18-091803-9 SN - 0083-5560 N1 - Serientitel: VDI-Berichte – Series title: VDI-Berichte IS - 1803 SP - 201 EP - 204 PB - VDI-Verl. CY - Düsseldorf AN - OPUS4-2893 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pech, S. A1 - Rehberg, M. A1 - Janke, R. A1 - Benndorf, D. A1 - Genzel, Y. A1 - Muth, Thilo A1 - Sickmann, A. A1 - Rapp, E. A1 - Reichl, U. T1 - Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins JF - Applied Microbiology and Biotechnology N2 - Adaptations of animal cells to growth in suspension culture concern in particular viral vaccine production, where very specific aspects of virus-host cell interaction need to be taken into account to achieve high cell specific yields and overall process productivity. So far, the complexity of alterations on the metabolism, enzyme, and proteome level required for adaptation is only poorly understood. In this study, for the first time, we combined several complex analytical approaches with the aim to track cellular changes on different levels and to unravel interconnections and correlations. Therefore, a Madin-Darby canine kidney (MDCK) suspension cell line, adapted earlier to growth in suspension, was cultivated in a 1-L bioreactor. Cell concentrations and cell volumes, extracellular metabolite concentrations, and intracellular enzyme activities were determined. The experimental data set was used as the input for a segregated growth model that was already applied to describe the growth dynamics of the parental adherent cell line. In addition, the cellular proteome was analyzed by liquid chromatography coupled to tandem mass spectrometry using a label-free protein quantification method to unravel altered cellular processes for the suspension and the adherent cell line. Four regulatory mechanisms were identified as a response of the adaptation of adherent MDCK cells to growth in suspension. These regulatory mechanisms were linked to the proteins caveolin, cadherin-1, and pirin. Combining cell, metabolite, enzyme, and protein measurements with mathematical modeling generated a more holistic view on cellular processes involved in the adaptation of an adherent cell line to suspension growth. KW - MDCK cell KW - Proteome KW - Metabolism KW - Enzyme activity KW - Suspension growth PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-522070 DO - https://doi.org/10.1007/s00253-021-11150-z VL - 105 IS - 5 SP - 1861 EP - 1874 PB - Springer AN - OPUS4-52207 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Goering, Harald A1 - Pötschke, P. A1 - Bhattacharyya, A.R. A1 - Janke, A. T1 - Melt mixing of polycarbonate / multi-wall carbon nanotube composites JF - Composite interfaces : proceedings KW - Multi-wall carbon nanotubes KW - Melt mixing KW - Electrical resistivity KW - Rheology PY - 2003 DO - https://doi.org/10.1163/156855403771953650 VL - 10 IS - 4-5 SP - 389 EP - 404 PB - North-Holland CY - New York, NY AN - OPUS4-2869 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -