TY - CONF A1 - Mayer, K. A1 - Chinta, P.K. A1 - Langenberg, K.-J. A1 - Krause, Martin T1 - Ultrasonic imaging of defects in known anisotropic and inmomogeneous structures with fast synthetic aperture methodes T2 - 18th WCNDT - World conference on nondestructive testing CY - Durban, South Africa DA - 2012-04-16 KW - SAFT KW - Pulse echo technique KW - Back propagation KW - Inverse scattering KW - Imaging PY - 2012 SN - 978-0-620-52872-6 SP - 1 EP - 20 AN - OPUS4-35101 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ballier, G. A1 - Mayer, K. A1 - Langenberg, K.-J. A1 - Schulze, Sebastian A1 - Krause, Martin T1 - Improvement on tendon duct examination by modelling and imaging with synthetic aperture and one-way inverse methods T2 - 18th WCNDT - World conference on nondestructive testing CY - Durban, South Africa DA - 2012-04-16 KW - SAFT KW - Ultrasonic NDT KW - Concrete KW - One way KW - AFIT KW - Time reversal PY - 2012 SN - 978-0-620-52872-6 SP - 1 EP - 8 AN - OPUS4-35102 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Konnertz, Nora A1 - Ding, Yi A1 - Harrison, W.J. A1 - Budd, P.M. A1 - Schönhals, Andreas A1 - Böhning, Martin T1 - Molecular mobility and gas transport properties of nanocomposites based on PIM-1 and polyhedral oligomeric phenethyl-silsesquioxanes (POSS) N2 - Polymers with intrinsic microporosity (PIMs) are of great interest in the field of gas separation membranes. Already the first synthesized PIM-1 shows extraordinary permeability and selectivity. Unfortunately, PIM-1 is susceptible to physical aging and thus gradually loses its outstanding properties. In this study a polyhedral oligomeric silsesquioxane with phenethyl substituents (PhenethylPOSS) was used as a nanofiller (0–40 wt%) in the PIM-1 matrix to potentially improve the gas transport properties and prevent physical aging. The molecular mobility of the solution-cast nanocomposite films was analyzed by Broadband Dielectric Spectroscopy (BDS). Furthermore, gas permeability was determined with the time lag method (0–20 bar upstream pressure) at 35 °C for N2, O2, CH4 and CO2. KW - Broadband Dielectric Spectroscopy KW - Gas separation KW - Gas permeation KW - Polymers of intrinsic microporosity KW - Nanocomposite KW - POSS KW - PIM-1 PY - 2017 DO - https://doi.org/10.1016/j.memsci.2017.02.007 SN - 0376-7388 SN - 1873-3123 VL - 529 SP - 274 EP - 285 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-39169 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Reifschneider, O. A1 - Vennemann, A. A1 - Buzanich, Günter A1 - Radtke, Martin A1 - Reinholz, Uwe A1 - Riesemeier, Heinrich A1 - Hogeback, J A1 - Köppen, C. A1 - Großgarten, M. A1 - Sperling, M. A1 - Wiemann, M. A1 - Karst, U. T1 - Revealing Silver Nanoparticle Uptake by Macrophages Using SR-μXRF and LA-ICP-MS N2 - To better study the impact of nanoparticles on both in vitro and in vivo models, tissue distribution and cellular doses need to be described more closely. Here silver nanoparticles were visualized in alveolar macrophages by means of synchrotron radiation micro X-ray fluorescence spectroscopy (SR-μXRF) with high spatial resolution of 3 × 3 μm2. For the spatial allocation of silver signals to cells and tissue structures, additional elemental labeling was carried out by staining with eosin, which binds to protein and can be detected as bromine signal with SR-μXRF. The method was compatible with immunostaining of macrophage antigens. We found that the silver distribution obtained with SR-μXRF was largely congruent with distribution maps from a subsequent laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) of the same tissue sites. The study shows a predominant, though not exclusive uptake of silver into alveolar macrophages in the rat lung, which can be modeled by a similar uptake in cultured alveolar macrophages. Advantages and limitations of the different strategies for measuring nanoparticle uptake at the single cell level are discussed. KW - Synchrotron KW - BAMline KW - XRF KW - Nanoparticle KW - Macrophagen PY - 2020 DO - https://doi.org/10.1021/acs.chemrestox.9b00507 VL - 33 IS - 5 SP - 1250 EP - 1255 PB - American Chemical Society AN - OPUS4-50855 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Konnertz, Nora A1 - Ding, Yi A1 - Harrison, W.J. A1 - Budd, P.M. A1 - Schönhals, Andreas A1 - Böhning, Martin T1 - Molecular mobility of the high performance membrane polymer PIM-1 as investigated by dielectric spectroscopy N2 - The increasing demand for energy efficient separation processes fosters the development of new high performance polymers as selective separation layers for membranes. PIM-1 is the archetypal representative of the class of polymers of intrinsic microporosity (PIM) which are considered most promising in this sector, especially for gas separations. Since their introduction, PIMs stimulated a vast amount of research in this field and meanwhile evolved to the state of the art in membrane technology for gas separation. The major obstacle for extending the practical membrane application is their strong tendency to physical aging. For the first time, investigations by broadband dielectric spectroscopy (BDS) addressing molecular dynamics and conductivity in PIM 1 are presented. As chain packing during film formation from the casting solution and physical aging are key factors determining the separation performance of PIMs as membrane materials, characterization of the molecular mobility in such materials as revealed by BDS will provide valuable information for further development and optimization. KW - PIM-1 KW - BDS broadband dielectric spectroscopy KW - Relaxation KW - Membrane PY - 2016 DO - https://doi.org/10.1021/acsmacrolett.6b00209 SN - 2161-1653 VL - 5 IS - 4 SP - 528 EP - 532 PB - ACS American Chemical Society CY - Washington, DC, USA AN - OPUS4-35798 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Krause, Martin A1 - Kurz, J.H. A1 - Lanata, F. A1 - Krstevska, L. A1 - Cavalli, A. T1 - Needs for further developing monitoring- and NDT-methods for timber structures N2 - For the assessmentand for monitoring of timber structures there are numerous promising methods that enable the quantitative description of the current condition of construction elements or parts of a structure. This concerns material properties like modulus of elasticity, moisture content and density as well as structural properties like dynamic characteristics, localization of inhomogeneities, cracks, and biological attack. During the work in COST Action FP 1101, since 2012 the capability of experimental methods are discussed and compared. This covers electrical and electromagnetic wave methods (resistivity, microwave, Radar, X-Ray), elastic waves (ultrasound, pile testing) and mechanical testing (static and dynamic). Describing and collecting the use of the experimental methods for monitoring purposes was the objective of working group 3 "Monitoring of Timber Structures" of the COST Action. Monitoring capabilities will extend the use of timber structures to further applications in civil engineering. So far, no standard exists but collections of application examples and review papers representing the state of the art. From there needs for further developments regarding monitoring and NDT methods for timber structures can be identified and these are described in this paper. T2 - SHATIS 2015 - 3rd International conference on structural health assessment of timber structures CY - Wroclaw, Poland DA - 09.09.2015 KW - Holz Konstruktionen KW - Monitoring KW - Langzeitverhalten KW - Erdbebensicherheit KW - Historische Bauwerke KW - Ultrasonic echo imaging KW - Historical timber structures KW - Seismic investigation KW - Dynamic tests KW - Anisotropic wave propagation KW - Extending service life PY - 2015 VL - 1 SP - 1 EP - 12 AN - OPUS4-35178 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Neumann, Patrick P. A1 - Kohlhoff, Harald A1 - Hüllmann, Dino A1 - Lilienthal, A.J. A1 - Kluge, Martin T1 - Bringing Mobile Robot Olfaction to the Next Dimension – UAV-based Remote Sensing of Gas Clouds and Source Localization N2 - This paper introduces a novel robotic platform for aerial remote gas sensing. Spectroscopic measurement methods for remote sensing of selected gases lend themselves for use on mini-copters, which offer a number of advantages for inspection and surveillance. No direct contact with the target gas is needed and thus the influence of the aerial platform on the measured gas plume can be kept to a minimum. This allows to overcome one of the major issues with gas-sensitive mini-copters. On the other hand, remote gas sensors, most prominently Tunable Diode Laser Absorption Spectroscopy (TDLAS) sensors have been too bulky given the payload and energy restrictions of mini-copters. Here, we introduce and present the Unmanned Aerial Vehicle for Remote Gas Sensing (UAV-REGAS), which combines a novel lightweight TDLAS sensor with a 3-axis aerial stabilization gimbal for aiming on a versatile hexacopter. The proposed system can be deployed in scenarios that cannot be addressed by currently available robots and thus constitutes a significant step forward for the field of Mobile Robot Olfaction (MRO). It enables tomographic reconstruction of gas plumes and a localization of gas sources. We also present first results showing the gas sensing and aiming capabilities under realistic conditions. T2 - IEEE International Conference on Robotics and Automation (ICRA) CY - Singapore DA - 29.05.2017 KW - Tunable Diode Laser Absorption Spectroscopy (TDLAS) KW - 3-axis gimbal KW - Mobile Robot Olfaction KW - Tomographic reconstruction of gas plumes KW - Localization of gas sources KW - Aerial platform PY - 2017 SN - 978-1-5090-4632-4 SP - 3910 EP - 3916 PB - IEEE AN - OPUS4-40545 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kuhne, Maren A1 - Dippong, Martin A1 - Flemig, Sabine A1 - Hoffmann, Katrin A1 - Petsch, K. A1 - Schenk, J.A. A1 - Kunte, Hans-Jörg A1 - Schneider, Rudolf T1 - Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA N2 - A novel method that optimizes the screening for antibody-secreting hapten-specific hybridoma cells by using flow cytometry is described. Cell clones specific for five different haptens were analyzed. We selectively double stained and analyzed fixed hybridoma cells with fluorophore-labeled haptens to demonstrate the target-selectivity, and with a fluorophore-labeled anti-mouse IgG antibody to characterize the level of surface expression of membrane-bound IgGs. ELISA measurements with the supernatants of the individual hybridoma clones revealed that antibodies from those cells, which showed the highest fluorescence intensities in the flow cytometric analysis, also displayed the highest affinities for the target antigens. The fluorescence intensity of antibody-producing cells corresponded well with the produced antibodies' affinities toward their respective antigens. Immunohistochemical staining verified the successful double labeling of the cells. Our method makes it possible to perform a high-throughput screening for hybridoma cells, which have both an adequate IgG production rate and a high target affinity. KW - Immunization KW - Hapten KW - Monoclonal antibodies KW - Hybridoma KW - Flow cytometry KW - ELISA KW - Estradiol KW - Estrone KW - Digoxigenin KW - Zearalenone KW - Aflatoxin KW - CLSM PY - 2014 DO - https://doi.org/10.1016/j.jim.2014.07.004 SN - 0022-1759 SN - 1872-7905 VL - 413 SP - 45 EP - 56 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-32322 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Fittschen, U.E.A. A1 - Menzel, M. A1 - Scharf, O. A1 - Radtke, Martin A1 - Reinholz, Uwe A1 - Buzanich, Günter A1 - Lopez, V.M. A1 - McIntosh, K. A1 - Streli, C. A1 - Havrilla, G.J. T1 - Observation of X-ray shadings in synchrotron radiation-total reflection X-ray fluorescence using a color X-ray camera N2 - Absorption effects and the impact of specimen shape on TXRF analysis has been discussed intensively. Model calculations indicated that ring shaped specimens should give better results in terms of higher counts per mass signals than filled rectangle or circle shaped specimens. One major reason for the difference in signal is shading effects. Full field micro-XRF with a color X-ray camera (CXC) was used to investigate shading, which occurs when working with small angles of excitation as in TXRF. The device allows monitoring the illuminated parts of the sample and the shaded parts at the same time. It is expected that sample material hit first by the primary beam shade material behind it. Using the CXC shading could be directly visualized for the high concentration specimens. In order to compare the experimental results with calculation of the shading effect the generation of controlled specimens is crucial. This was achieved by 'drop on demand' technology. It allows generating uniform, microscopic deposits of elements. The experimentally measured shadings match well with those expected from calculation. KW - TXRF KW - Full field micro-XRF KW - Color X-ray camera PY - 2014 DO - https://doi.org/10.1016/j.sab.2014.06.025 SN - 0584-8547 SN - 0038-6987 VL - 99 SP - 179 EP - 184 PB - Elsevier CY - Amsterdam AN - OPUS4-31180 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -