TY - JOUR A1 - Werner, L. A1 - Tetz, M. A1 - Feldmann, Ines A1 - Bücker, Michael T1 - Evaluating and defining the sharpness of intraocular lenses: Microedge structure of commercially available square-edged hydrophilic intraocular lenses JF - Journal of cataract and refractive surgery KW - ESEM KW - Intraocularlinse KW - IOL PY - 2009 DO - https://doi.org/10.1016/j.jcrs.2008.11.042 SN - 0886-3350 VL - 35 IS - 3 SP - 556 EP - 566 PB - Elsevier CY - New York, NY, USA AN - OPUS4-19228 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Völzke, Jule L. A1 - Hodjat Shamami, Parya A1 - Gawlitza, Kornelia A1 - Feldmann, Ines A1 - Zimathies, Annett A1 - Meyer, Klas A1 - Weller, Michael G. T1 - High-Purity Corundum as Support for Affinity Extractions from Complex Samples JF - Separations N2 - Nonporous corundum powder, known as an abrasive material in the industry, was functionalized covalently with protein binders to isolate and enrich specific proteins from complex matrices. The materials based on corundum were characterized by TEM, ESEM, BET, DLS, EDS, and zeta potential measurements. The strong Al-O-P bonds between the corundum surface and amino phosphonic acids were used to introduce functional groups for further conjugations. The common crosslinker glutaraldehyde was compared with a hyperbranched polyglycerol (PG) of around 10 kDa. The latter was oxidized with periodate to generate aldehyde groups that can covalently react with the amines of the surface and the amino groups from the protein via a reductive amination process. The amount of bound protein was quantified via aromatic amino acid analysis (AAAA). This work shows that oxidized polyglycerol can be used as an alternative to glutaraldehyde. With polyglycerol, more of the model protein bovine serum albumin (BSA) could be attached to the surface under the same conditions, and lower non-specific binding (NSB) was observed. As a proof of concept, IgG was extracted with protein A from crude human plasma. The purity of the product was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A binding capacity of 1.8 mg IgG per gram of corundum powder was achieved. The advantages of corundum include the very low price, extremely high physical and chemical stability, pressure resistance, favorable binding kinetics, convenient handling, and flexible application. KW - Corundum KW - Sapphire KW - Affinity chromatography KW - Antibodies KW - Self-assembled monolayers (SAM) KW - Polyglycerol KW - Dendrimer KW - Nonspecific binding (NSB) KW - Purification KW - Solid-phase extraction (SPE) PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-559342 DO - https://doi.org/10.3390/separations9090252 VL - 9 IS - 9 SP - 1 EP - 13 PB - MDPI CY - Basel AN - OPUS4-55934 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Völzke, Jule L. A1 - Hodjat Shamami, P. A1 - Gawlitza, Kornelia A1 - Feldmann, Ines A1 - Zimathies, Annett A1 - Weller, Michael G. T1 - High-purity corundum as support for affinity extractions from complex samples T2 - Preprints N2 - Nonporous corundum powder, known as an abrasive material in the industry, was functionalized covalently with protein binders to isolate and enrich specific proteins from complex matrices. The materials based on corundum were characterized by TEM, ESEM, BET, DLS, and zeta potential measurements. The strong Al-O-P bonds between the corundum surface and amino phosphonic acids are used to introduce functional groups for further conjugations. The common cross-linker glutaraldehyde was compared with a hyperbranched polyglycerol (PG) of around 10 kDa. The latter is oxidized with periodate to generate aldehyde groups that can covalently react with the amines of the surface and the amino groups from the protein via a reductive amination process. The amount of bound protein was quantified via aromatic amino acid analysis (AAAA). This work shows that oxidized polyglycerol can be used as an alternative to glutaraldehyde. With polyglycerol, more of the model protein bovine serum albumin (BSA) could be attached to the surface under the same conditions, and lower nonspecific binding (NSB) was observed. As a proof of concept, IgG was extracted with protein A from crude human plasma. The purity of the product was examined by SDS-PAGE. A binding capacity of 1.8 mg IgG per g of corundum powder was achieved. The advantages of corundum are the very low price, extremely high physical and chemical stability, pressure resistance, favorable binding kinetics, and flexible application. KW - Protein KW - Bioseparation KW - Purification KW - Immunoprecipitation KW - Affinity chromatography KW - Polyglycerol KW - Glutaraldehyde KW - Linker KW - Bioconjugation KW - Self-assembled monolayer (SAM) KW - Periodate oxidation KW - Reductive amination KW - Antibodies KW - Igg KW - Immunoglobulins KW - Carrier KW - Solid phase KW - Hyperbranched polymer KW - Aromatic amino acid analysis aaaa PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-555142 DO - https://doi.org/10.20944/preprints202208.0004.v1 SP - 1 EP - 13 PB - MDPI CY - Basel AN - OPUS4-55514 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Torge, Manfred A1 - Sabel, Martin A1 - Feldmann, Ines A1 - Kohl, Anka T1 - Naturwissenschaftliche Untersuchungen im Rahmen des Projektes 'Konservierung mittelalterlicher Glasmalerei im Kontext spezieller materieller und umweltbedingter Gegebenheiten' T2 - Bauforschung - Denkmalpflege 'Konservierung mittelalterlicher Glasmalerei im Kontext spezieller materieller und umweltbedingter Gegebenheiten' N2 - Nachsicherung geschädigter Zaponlack-Wachs-Sicherungen an den um 1410/20 entstandenen Glasmalereien des Helenafensters nord VIII am Erfurter Dom - Untersuchungen zu Schadensursachen, Schadensphänomenen, Methoden der Nachbehandlung und des Monitorings KW - Glasmalerei KW - Restaurierung KW - Zaponlack PY - 2011 SN - 978-3-8167-8470-8 VL - D 1012 SP - 36 EP - 75 PB - Fraunhofer IRB Verl. CY - Stuttgart AN - OPUS4-23631 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Torge, Manfred A1 - Müller, Wolfgang A1 - Adam, Karin A1 - Feldmann, Ines A1 - Kruschke, Detlef T1 - Verbräunung mittelalterlicher Glasmalereien JF - Restauro KW - Kulturguterhaltung KW - Hydrazin KW - Verbräunung PY - 2003 SN - 0933-4017 VL - 109 IS - 8 SP - 582 EP - 588 PB - Callwey CY - München AN - OPUS4-3249 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Torge, Manfred A1 - Krug, Sonja A1 - Bücker, Michael A1 - Feldmann, Ines A1 - Scharf, Holger A1 - Witthuhn, Heike A1 - Sander, C. A1 - Fraenkler-Balhorn, K. ED - Roemich, H. T1 - Mercury emissions from historical tin amalgam mirrors T2 - Interim Meeting of the ICOM-CC Working Group - Glass & Ceramics Conservation (Proceedings) T2 - Interim Meeting of the ICOM-CC Working Group - Glass & Ceramics Conservation CY - Corning, New York, USA DA - 2010-10-03 KW - Mirrors KW - Mercury KW - Tin amalgam KW - Deterioration PY - 2010 SN - 978-0-87290-182-7 SP - 156 EP - 163 AN - OPUS4-22175 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Torge, Manfred A1 - Krug, Sonja A1 - Bücker, Michael A1 - Feldmann, Ines A1 - Scharf, Holger A1 - Witthuhn, Heike A1 - Sander, C. T1 - Flüchtiges Quecksilber - Emission von Quecksilber aus historischen Zinnamalgamspiegeln JF - Restauro N2 - Mit der Erfindung des Glas-Guß-Walzverfahrens eröffnete sich die Möglichkeit, größere Spiegelflächen als zuvor herzustellen. Diese sogenannten Quecksilberspiegel sind mit Zinnamalgam beschichtet. Wenn Restauratoren mit Zinnamalgamspiegeln arbeiten, müssen sie strenge Sicherheitsvorkehrungen einhalten. Häufig finden sich im Spiegelrahmen Quecksilberkugeln, die beim Öffnen herausfallen und damit gesundheitsschädliches Quecksilber freisetzen können. In einem Forschungsprojekt wurden die Quecksilberemissionen aus Zinnamalgamspiegeln gemessen und Möglichkeiten entwickelt, diese zu verringern. KW - Quecksilber KW - Emission KW - Zinnamalgamspiegel PY - 2012 SN - 0933-4017 VL - 118 IS - 3 SP - 30 EP - 37 PB - Callwey CY - München AN - OPUS4-25846 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Torge, Manfred A1 - Krug, Sonja A1 - Bücker, Michael A1 - Feldmann, Ines A1 - Scharf, Holger A1 - Witthuhn, Heike T1 - Investigation of mercury emissions of historic tin-mercury-mirrors JF - The e-journal of nondestructive testing & ultrasonics N2 - With the implementation of glass casting in France in the second half of the 17th century, larger mirrors could be produced. So-called tin-mercury mirrors were made by coating glass with tin amalgam. Today, many historical mirrors are partly damaged, the image quality is compromised, and the material integrity of the mirror is threatened. The transformation from tin into an oxide starts at the surface of the amalgam and proceeds down to the glass surface. The mercury dissolves as a liquid or gaseous phase. The amalgam layer is destroyed, so that conservation is necessary. Conservators must follow strict safety precautions while handling amalgam mirrors because of possible mercury emissions and corrosion of the amalgam, during which elemental mercury accumulates near the mirror frame in droplets and is emitted into the air. In a research project, the quantity of the mercury emissions from historical mirrors was examined and a technique to decrease these emissions was developed. T2 - International Conference on Non-destructive Investigations and Microanalysis for the Diagnostics and Conservation of Cultural and Environmental Heritage (ART 2011) CY - Florence, Italy DA - 13.04.2011 KW - Emission KW - Quecksilber KW - Zinn-Amalgamspiegel PY - 2011 UR - https://www.ndt.net/search/docs.php3?id=11425 SN - 1435-4934 VL - 16 IS - 10 SP - 1 EP - 8 PB - NDT.net CY - Kirchwald AN - OPUS4-25270 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Torge, Manfred A1 - Krug, Sonja A1 - Bücker, Michael A1 - Feldmann, Ines A1 - Scharf, Holger A1 - Witthuhn, Heike T1 - Investigation of mercury emissions of historic tin-mercury mirrors T2 - ART 2011 -10th International Conference on non-destructive investigations and microanalysis for the diagnostics and conservation of cultural and environmental heritage N2 - With the implementation of glass casting in France in the second half of the 17th century, larger mirrors could be produced. So-called tin-mercury mirrors were made by coating glass with tin amalgam. Today, many historical mirrors are partly damaged, the image quality is compromised, and the material integrity of the mirror is threatened. The transformation from tin into an oxide starts at the surface of the amalgam and proceeds down to the glass surface. The mercury dissolves as a liquid or gaseous phase. The amalgam layer is destroyed, so that conservation is necessary. Conservators must follow strict safety precautions while handling amalgam mirrors because of possible mercury emissions and corrosion of the amalgam, during which elemental mercury accumulates near the mirror frame in droplets and is emitted into the air. In a research project, the quantity of the mercury emissions from historical mirrors was examined and a technique to decrease these emissions was developed. T2 - ART 2011 -10th International Conference on non-destructive investigations and microanalysis for the diagnostics and conservation of cultural and environmental heritage CY - Florence, Italy DA - 13.04.2011 KW - Zinn-Amalgamspiegel KW - Quecksilber KW - Emission PY - 2011 SP - 1 EP - 8 AN - OPUS4-23879 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Torge, Manfred A1 - Feldmann, Ines A1 - Müller, Wolfgang T1 - Malschichtsicherung auf Wachsbasis T2 - Mittelalterliche Glasmalerei im Dom zu Erfurt KW - Kulturguterhaltung KW - Bienenwachs KW - Carnaubawachs PY - 2004 SN - 3-361-00577-9 SP - 87 EP - 92 PB - Ed. Leipzig CY - Leipzig AN - OPUS4-3460 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -