TY - JOUR A1 - Köppen, Robert A1 - Bremser, Wolfram A1 - Stephan, Ina A1 - Klein-Hartwig, Karin A1 - Rasenko, Tatjana A1 - Koch, Matthias T1 - T-2 and HT-2 toxins in oat flakes: development of a certified reference material N2 - Trichothecene mycotoxins, with T-2 and HT-2 toxins being the main representatives of the type A subgroup, are naturally and worldwide occurring contaminants frequently found in grain-based food and feed. Due to the high consumption of these products and the potential health risk associated herewith, concerns about the safety and quality of food and feed have increased over the last decades at both governmental and consumer levels. Since it is not possible to avoid their occurrence, tremendous efforts have been performed to identify and monitor mycotoxins in food and feed to make their consumption safe. However, suitable certified reference materials (CRMs) intended for quality assurance and quality control purposes are still lacking for many mycotoxin-matrix combinations. Therefore, in the framework of a European Reference Material (ERM®) project, the first CRM for T-2 and HT-2 toxin in ground oat flakes (ERM®-BC720) was developed according to the requirements of ISO Guide 35. The whole process of ERM®-BC720 development, including sample preparation, homogeneity and stability studies and value assignment, is presented. The assignment of the certified mass fractions was based upon an in-house study using high-performance liquid chromatography isotope-dilution tandem mass spectrometry. Simultaneously, an interlaboratory comparison study involving 24 expert laboratories was conducted in order to support the in-house certification study. The certified values and their corresponding expanded uncertainties (k=2) for both T-2 and HT-2 toxin in ERM®-BC720, traceable to the international system of units, are (82±4) µg kg-1 and (81±4) µg kg-1, respectively. KW - Type A trichothecenes KW - Fusarium toxins KW - Isotope-dilution mass spectrometry KW - Food analysis KW - European Reference Material KW - Quality assurance PY - 2015 U6 - https://doi.org/10.1007/s00216-014-8251-4 SN - 1618-2642 SN - 1618-2650 VL - 407 IS - 11 SP - 2997 EP - 3007 PB - Springer CY - Berlin AN - OPUS4-33073 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gillatt, J. A1 - Julian, K. A1 - Brett, K. A1 - Goldbach, M. A1 - Grohmann, J. A1 - Heer, B. A1 - Nichols, K. A1 - Roden, K. A1 - Rook, T. A1 - Schubert, T. A1 - Stephan, Ina T1 - The microbial resistance of polymer dispersions and the efficacy of polymer dispersion biocides - A statistically validated method N2 - The biodeterioration of water-based manufactured formulations, such as polymer dispersions, is a major problem for the producing companies and the users of such products. Industrial preservatives, also known as biocides, are therefore used to protect these and similar products from the effects of microorganisms, predominantly bacteria and yeasts. In the absence of internationally recognised standard methods for determining the resistance of polymer dispersions to microbial growth and the efficacy of biocides used in them, protocols for testing other products, e.g., paints, have been adapted, and other methods produced by manufacturing companies, test laboratories, and academic institutions have been used. Often these do not take into account the specific nature of the materials being tested, the types of organism commonly causing contamination, and the conditions that the polymer dispersions will be exposed to during manufacture, storage, and use. By conducting a series of round-robin, collaborative tests, the member organisations of the International Biodeterioration Research Group Polymer Dispersion Working Group have identified the bacteria that commonly infect polymer dispersions, defined the main parameters necessary for a standard method of test, and developed a protocol that is robust, repeatable, and reproducible. The recommended test involves three repeat inoculations of the material with a previously determined mix of seven Gram-negative bacteria and evaluation of living cells by a simple plating technique. The work reported here, carried out by nine participating laboratories, is a final statistical validation and suggests that the method is eminently suitable as a standard test method. KW - Biocides KW - Biodeterioration KW - IBRG KW - Polymer dispersions KW - Test method PY - 2015 U6 - https://doi.org/10.1016/j.ibiod.2015.04.028 SN - 0964-8305 VL - 104 SP - 32 EP - 37 PB - Elsevier CY - Barking AN - OPUS4-33852 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Stephan, Ina T1 - Wirksamkeitsstudien bezogen auf die Auslobung von biozidhaltigen Kunststoffen T2 - IK 2015 - Biozide - Materialien, Anwendungen und Trends CY - Südliches Anhalt OT Weißandt-Gölzau, Deutschland DA - 2015-10-01 PY - 2015 AN - OPUS4-35099 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Schoknecht, Ute A1 - Plarre, Rüdiger A1 - Stephan, Ina ED - Lang, A. ED - Zimmer, J. T1 - Einführung in die biologische Wirksamkeitsprüfung von Bioziden im Materialschutz KW - Biozide KW - Materialschutz KW - Wirksamkeitsprüfung KW - Biocide KW - Material preservation KW - Efficiency test PY - 2015 SN - 9783861021940 SP - 48 EP - 50 PB - Deutsches Historisches Museum CY - Berlin AN - OPUS4-35411 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -