TY - JOUR A1 - Raysyan, Anna A1 - Eremin, S. A. A1 - Beloglazova, N. V. A1 - De Saeger, S. A1 - Gravel, I. V. T1 - Immunochemical approaches for detection of aflatoxin B1 in herbal medicines N2 - Introduction Aflatoxin B1 (AFB1) is a toxic low‐molecular‐weight secondary metabolite of Aspergillus flavus and A. parasiticus. AFB1 was classified as a Group I carcinogen by the World Health Organisation for Research on Cancer in 1993. AFB1 is an unavoidable natural contaminant of some herbal medicine, able to cause serious health issues for humans consuming the related medicine. Objective Therefore, this study aimed to develop an efficient fluorescence polarisation immunoassay (FPIA) and a rapid, low‐cost, and easy‐to‐use membrane‐based flow‐through immunoassay (MBA) for determination of AFB1 in herbal medicine Origanum vulgare L., Rubus idaeus L., Urtica dioica L. and Sorbus aucuparia L. Results A cut‐off level of the developed MBA was 0.8 ppb. Validation of the developed test was performed with blank and spiked samples. Using three naturally contaminated or three artificially spiked samples. The FPIA showed a linear working range of 8.6 to 64 ppb, and a half maximal inhibitory concentration (IC50) of 24 ppb. Conclusion The results were in good correlation with the enzymelinked immunosorbent assay (ELISA) results (the IC50 0.1 ppb). Both the sample preparation and analysis are simple, cost‐effective and easy to perform on‐site in non‐laboratory environments. Liquid chromatography with tandem mass spectrometry (LC‐MS/MS) was used as a confirmatory technique. KW - Fluorescence Polarisation Immunoassay KW - membrane-based flow-through immunoassay KW - Mycotoxins KW - Mykotoxine KW - Schnelltest PY - 2020 DO - https://doi.org/10.1002/pca.2931 SN - 1099-1565 VL - 31 IS - 5 SP - 662 EP - 669 PB - Wiley Online Libary CY - Hoboken, NJ, USA AN - OPUS4-51341 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Filimonova, S. M. A1 - Melnikov, E. S. A1 - Kaufmann, Jan Ole A1 - Shchepochkina, O. Y. A1 - Eremin, S. A. A1 - Gravel, I. V. A1 - Raysyan, Anna T1 - Exploring the anti‐α‐amylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDI‐TOF‐MS and LC–MS/MS approach N2 - A combination of TLC-bioautography, MALDI-TOF-MS and LC–MS/MS methods was used to identify flavonoids with anti-α-amylase activity in extracts of Lathyrus pratensis L. (herb), L. polyphillus L. (fruits), Thermopsis lanceolata R. Br. (herb) and S. japonica L. (buds). After the TLC-autobiography assay, substances with anti-amylase activity were identified by MALDI-TOF-MS followed by confirmation of the result by LC–MS/MS. Results of the study revealed that the flavonoids apigenin, luteolin, formononetin, genistein and kaempferol display marked anti-α-amylase activity. Formononetin showed the largest activity. Compared with LC–MS/MS, MALDI-TOF-MS is a quick and convenient method; results can be obtained within minutes; and only minor sample amounts are required which allows us to analyse mixtures of substances without preliminary separation. However, the inability to distinguish between isomers is the main limitation of the method. KW - Enzyme KW - MALDI-TOF-MS KW - LC-MS/MS KW - Massenspektrometrie PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-577128 DO - https://doi.org/https://doi.org/10.1111/ijfs.16491 SN - 0950-5423 VL - 58 IS - 7 SP - 3902 EP - 3911 PB - Wiley & Sons CY - Hoboken, NJ, USA AN - OPUS4-57712 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -