TY - JOUR A1 - Sachse, André A1 - Hasenbein, Ines A1 - Hortschansky, Peter A1 - Schmuck, Klaus D. A1 - Maenz, Stefan A1 - Illerhaus, Bernhard A1 - Kuehmstedt, Peter A1 - Ramm, Roland A1 - Huber, René A1 - Kunisch, Elke A1 - Horbert, Victoria A1 - Gunnella, Francesca A1 - Roth, Andreas A1 - Schubert, Harald A1 - Kinne, Raimund W. T1 - BMP-2 (and partially GDF-5) coating significantly accelerates and augments bone formation close to hydroxyapatite/tricalcium-phosphate/brushite implant cylinders for tibial bone defects in senile, osteopenic sheep N2 - Bilateral defects (diameter 8 mm) in the medial tibial head of senile, osteopenic female sheep (n = 48; 9.63 ± 0.10 years; mean ± SEM) were treated with hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP)/dicalcium phosphate dihydrate (DCPD; brushite) cylinders coated with BMP-2 (25 or 250 micrograms) or growth differentiation factor (GDF)-5 (125 or 1250 micrograms; left side); cylinders without BMP served as controls (right side). Three, 6, and 9 months post-operation (n = 6 each group), bone structure and formation were analyzed in vivo by X-ray and ex vivo by osteodensitometry, histomorphometry, and micro-computed tomography (micro-CT) at 3 and 9 months. Semi-quantitative X-ray evaluation showed significantly increasing bone densities around all implant cylinders over time. High-dose BMP-2-coated cylinders (3 and 9 months) and low-dose GDF-5-coated cylinders (3 and 6 months) demonstrated significantly higher densities than controls (dose-dependent for BMP-2 at 3 months). This was confirmed by osteodensitometry at 9 months for high-dose BMP-2-coated cylinders (and selected GDF-5 groups), and was again dose-dependent for BMP-2. Osteoinduction by BMP-2 was most pronounced in the adjacent bone marrow (dynamic histomorphometry/micro-CT). BMP-2 (and partially GDF-5) significantly increased the bone formation in the vicinity of HA/TCP/DCPD cylinders used to fill tibial bone defects in senile osteopenic sheep and may be suitable for surgical therapy of critical size, non-load-bearing bone defects in cases of failed tibial head fracture or defect healing. KW - Bone morphogenetic protein (BMP) KW - Growth differentiation factor (GDF) KW - Bone replacement material PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-623923 DO - https://doi.org/10.1007/s10856-023-06734-2 SN - 1573-4838 VL - 34 IS - 7 SP - 1 EP - 15 PB - Springer Science and Business Media LLC CY - Dordrecht AN - OPUS4-62392 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -