TY - JOUR A1 - Santos de Freitas, M. A1 - Araghi, R. R. A1 - Brandenburg, E. A1 - Leiterer, Jork A1 - Emmerling, Franziska A1 - Folmert, K. A1 - Gerling-Driessen, U. I. M. A1 - Bardiaux, B. A1 - Böttcher, C. A1 - Pagel, K. A1 - Diehl, A. A1 - v. Berlepsch, H. A1 - Oschkinat, H. A1 - Koksch, B. T1 - The protofilament architecture of a de novo designed coiled coil-based amyloidogenic peptide N2 - Amyloid fibrils are polymers formed by proteins under specific conditions and in many cases they are related to pathogenesis, such as Parkinson’s and Alzheimer’s diseases. Their hallmark is the presence of a β-sheet structure. High resolution structural data on these systems as well as information gathered from multiple complementary analytical techniques is needed, from both a fundamental and a pharmaceutical perspective. Here, a previously reported de novo designed, pH-switchable coiled coil-based peptide that undergoes structural transitions resulting in fibril formation under physiological conditions has been exhaustively characterized by transmission electron microscopy (TEM), cryo-TEM, atomic force microscopy (AFM), wide-angle X-ray scattering (WAXS) and solid-state NMR (ssNMR). Overall, a unique 2-dimensional carpet-like assembly composed of large coexisiting ribbon-like, tubular and funnel-like structures with a clearly resolved protofilament substructure is observed. Whereas electron microscopy and scattering data point somewhat more to a hairpin model of β-fibrils, ssNMR data obtained from samples with selectively labelled peptides are in agreement with both, hairpin structures and linear arrangements. KW - Amyloid KW - Elektronenmikroskopie PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-458713 UR - https://www.sciencedirect.com/science/article/pii/S1047847718301333 DO - https://doi.org/10.1016/j.jsb.2018.05.009 SN - 1047-8477 VL - 203 IS - 3 SP - 263 EP - 272 PB - Elsevier AN - OPUS4-45871 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Thünemann, Andreas A1 - Kubowicz, S. A1 - v. Berlepsch, H. A1 - Möhwald, H. T1 - Two-Compartment Micellar Assemblies Obtained via Aqueous Self-Organization of Synthetic Polymer Building Blocks N2 - We synthesized a symmetric linear ABCBA pentablock copolymer consisting of poly(ethylene oxide), poly(-benzyl L-glutamate), and a poly(perfluoro ether) (fluorolink). The different blocks are highly immiscible with each other and form two-compartment micelles of mainly cylindrical shape in aqueous solution with lengths in the range of 100 to 200 nm and diameters of about 24 nm. The poly(perfluoro ether) (C blocks) forms the liquidlike center of the micelles (d = 6 nm). This is surrounded by a first shell of ca. 2 nm thickness consisting of -sheets of poly(-benzyl L-glutamate) (B blocks) and a second 7 nm shell of poly(ethylene oxide) (A blocks). The A blocks provide water solubility, and the B and C blocks form separated hydrophobic compartments. This work is a contribution to the development of multicompartment micelles devoted to mimic transport proteins such as serum albumins in long-term development. KW - Micelles KW - Block-copolymers PY - 2006 DO - https://doi.org/10.1021/la0533720 SN - 0743-7463 SN - 1520-5827 VL - 22 IS - 6 SP - 2506 EP - 2510 PB - American Chemical Society CY - Washington, DC AN - OPUS4-12190 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Reck, Günter A1 - Dähne, Siegfried A1 - Kirstein, S. A1 - v. Berlepsch, H. A1 - Böttcher, C. A1 - Burger, C. A1 - Ouart, A. T1 - Chiral J-aggregates formed by achiral cyanine dyes PY - 2000 UR - http://www3.interscience.wiley.com/cgi-bin/abstract/74500665/ABSTRACT DO - https://doi.org/10.1002/1439-7641(20001103)1:3<146::AID-CPHC146>3.0.CO;2-Y SN - 1439-4235 SN - 1439-7641 VL - 1 IS - 3 SP - 146 EP - 150 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-865 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Lutz, J.-F. A1 - Kubowicz, St. A1 - Baussard, J.-F. A1 - Thünemann, Andreas A1 - v. Berlepsch, H. A1 - Laschewsky, A. T1 - Multicompartment Micelles Obtained via the Self-Assembly of a Well-Defined Triblock Macrosurfactant Prepared by RAFT Polymerization KW - Micelles KW - Living polymerization KW - RAFT KW - Block copolymers PY - 2005 SN - 0032-3934 VL - 46 IS - 2 SP - 297 EP - 298 PB - American Chemical Society CY - Newark, NJ AN - OPUS4-10534 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kubowicz, St. A1 - Baussard, J.-F. A1 - Lutz, J.-F. A1 - Thünemann, Andreas A1 - v. Berlepsch, H. A1 - Laschewsky, A. T1 - Multicompartment Micelles Formed by Self-Assembly of Linear ABC Triblock Copolymers in Aqueous Medium KW - Small-angle x-ray scattering KW - Micelles PY - 2005 DO - https://doi.org/10.1002/anie.200500584 SN - 1433-7851 SN - 1521-3773 SN - 0570-0833 VL - 44 SP - 5262 EP - 5265 PB - Wiley-VCH CY - Weinheim AN - OPUS4-10537 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dähne, Siegfried A1 - v.Berlepsch, H. A1 - Böttcher, C. A1 - Quart, A. A1 - Regenbrecht, M. A1 - Akari, S. A1 - Keiderling, U. A1 - Schnablegger, H. A1 - Kirstein, S. T1 - Surfactant-induced changes of morphology of J-aggregates: Superhelix-to-tubule transformation N2 - The morphology of the J-aggregates formed after the addition of ionic surfactants into solutions of the 3,3'-bis(3-carboxypropyl)-5,5',6,6'-tetrachloro-1,1'-dioctylbenzimidacarbocyanine dye (C8O3) was characterized by absorption and circular dichroism spectroscopy, cryogenic temperature transmission electron microscopy, scanning force microscopy, and small-angle neutron scattering. The optical activity of the C8O3 J-aggregates, existing in aqueous solution as superhelices composed of tubular single strands, disappears upon addition of both cationic and anionic surfactants. This is accompanied by distinct spectral changes in the visible region as well as changes of the aggregates' morphology. The anionic surfactant sodium dodecyl sulfate induces the formation of single-walled tubules of 15 nm diameter and 300-600 nm length, which completely transform after several days into thick multilamellar tubes of micrometer length. The cationic surfactant trimethyltetradecylammonium bromide first produces vesicles that later transform again into tubular aggregates of nanometer thickness and micrometer length. PY - 2000 DO - https://doi.org/10.1021/la000014i SN - 0743-7463 SN - 1520-5827 VL - 16 IS - 14 SP - 5908 EP - 5916 PB - American Chemical Society CY - Washington, DC AN - OPUS4-863 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dähne, Siegfried A1 - v.Berlepsch, H. A1 - Böttcher, C. A1 - Quart, A. A1 - Burger, C. A1 - Kirstein, S. T1 - Supramolecular Structures of J-Aggregates of Carbocyanine Dyes in Solution N2 - Light- and small-angle neutron scattering as well as cryo-transmission electron microscopy (cryo-TEM) studies were performed to probe the structure of J-aggregates formed by a series of achiral dye molecules of the 5,5',6,6'-tetrachlorobenzimidacarbocyanine chromophore having 1,1'-dialkyl substituents combined with 3,3'-bis(4-sulfobutyl)-3,3'-bis(4-carboxybutyl) or 3,3'-bis(3-carboxypropyl) substituents. Assemblies that display a dependence on the substituents different complex supramolecular structures of nanometer-to-micrometer size have been directly visualized by cryo-TEM. The superstructures span from monomolecular layers formed by the 1,1'-diethyl-3,3'-bis(4-sulfobutyl) derivative and stacks of bilayer ribbons in the case of the 1,1'-dioctyl-3,3'-bis(4-carboxybutyl) derivative to twisted ropelike structures for the chiral aggregate of the 1,1'-dioctyl-3,3'-bis(3-carboxypropyl)-substituted chromophore. PY - 2000 DO - https://doi.org/10.1021/jp000220z SN - 1520-6106 SN - 1520-5207 SN - 1089-5647 VL - 104 IS - 22 SP - 5255 EP - 5262 PB - Soc. CY - Washington, DC AN - OPUS4-862 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -