TY - JOUR A1 - Knabe, Ch. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Howlett, C.R. A1 - Markovic, B. A1 - Zreiqat, H. T1 - The functional expression of human bone-derived cells grown on rapidly resorbable calcium phosphate ceramics JF - Biomaterials N2 - The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation, since it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates on the expression of bone-related genes and proteins by human bone-derived cells (HBDC) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were a-TCP, and four materials which were created from ß-Rhenanite and its derivatives: R1-ß-Rhenanite (CaNaPO4); R1/M2 composed of CaNaPO4 and MgNaPO4; R1+SiO2 composed of CaNaPO4 and 9% SiO2 (wt%); and R17-Ca2KNa(PO4)2. HBDC were grown on the substrata for 3, 5, 7, 14 and 21 days, counted and probed for various mRNAs and proteins (Type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrata supported continuous cellular growth for 21 days. At day 21, surfaces of R1+SiO2 and R17 had the highest number of HBDC. At 14 and 21 days, cells on R1 and on R1+SiO2 displayed significantly enhanced expression of all osteogenic proteins. Since all novel calcium phosphates supported cellular proliferation together with expression of bone-related proteins at least as much as TCP, these ceramics can be regarded as potential bone substitutes. R1 and R1+SiO2 had the most effect on osteoblastic differentiation, thus suggesting that these materials may possess a higher potency to enhance osteogenesis than TCP. KW - Calcium phosphate ceramics KW - Human bone-derived cells KW - Cell-biomaterial interactions KW - Bone substitutes KW - In situ hybridization PY - 2004 DO - https://doi.org/10.1016/S0142-9612(03)00525-8 SN - 0142-9612 VL - 25 IS - 2 SP - 335 EP - 344 PB - Elsevier CY - Oxford AN - OPUS4-2957 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knabe, Ch. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Klar, F. A1 - Zreiqat, H. T1 - The modulation of osteogenesis in vitro by calcium titanium phosphate coatings JF - Biomaterials N2 - Calcium phosphate coated titanium and titanium alloy are widely used as dental and orthopaedic implants. This study examines the effect of novel calcium titanium and calcium titanium zirconium phosphates suitable for plasma-spraying onto titanium substrata on the expression of bone-related genes and proteins by human bone-derived cells (HBDC) and compares this behavior to that on native titanium and hydroxyapatite-coated titanium. Test materials were an acid etched and sand-blasted titanium surface (Ti-DPS), a plasma-sprayed hydroxyapatite coating (HA), and five materials which were created from CaTi4(PO4)6 (CTP) and CaZr4(PO4)6 (CZP): sintered CaTi4(PO4)6 (CTP-S1), sintered 46CaO·23TiO2·31P2O5 (CTP-S2), sintered CaTiZr3(PO4)6, (CTZP-S1), sintered 46CaO·23ZrO2·31P2O5 (CTZP-S2) and sintered 55CaO·20TiO2·31P2O5 (CTP-S3). HBDC were grown on the substrata for 3, 7, 14 and 21 d, counted and probed for various mRNAs and proteins (type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrates significantly affected cellular growth and the temporal expression of an array of bone-related genes and proteins. At 14 and 21 d, cells on CTP-S3 displayed significantly enhanced expression of all osteogenic mRNAs. Surfaces of CTP-S1 and CTP-S3 had the most effect on osteoblastic differentiation inducing a greater expression of an array of osteogenic markers than recorded for cells grown on Ti-DPS and HA, suggesting that these novel materials may possess a higher potency to enhance osteogenesis. KW - Calcium phosphate coatings KW - Osteoblast KW - In vitro test KW - In situ hybridization KW - Osteogenesis PY - 2004 DO - https://doi.org/10.1016/j.biomaterials.2004.01.059 SN - 0142-9612 VL - 25 IS - 20 SP - 4911 EP - 4919 PB - Elsevier CY - Oxford AN - OPUS4-3501 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knabe, C. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Meyer, J. A1 - Howlett, C. A1 - Markovic, B. A1 - Zreiqat, H. T1 - Effect of rapidly resorbable calcium phosphates and a calcium phosphate bone cement on the expression of bone-related genes and proteins in vitro JF - Journal of biomedical materials research / A N2 - The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation because it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates and a calcium phosphate bone cement on the expression of bone-related genes and proteins by human bone-derived cells (HBDCs) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were -TCP, two materials with a crystalline phase Ca2KNa(PO4)2 and with a small amorphous portion containing either magnesium potassium phosphate (material denominated GB14) or silica phosphate (material denominated GB9), and a calcium phosphate bone cement (material denominated Biocement D). HBDCs were grown on the substrata for 3, 7, 14, and 21 days, counted, and probed for various mRNAs and proteins (type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase, and bone sialoprotein). All substrates supported continuous cellular growth for 21 days. In the presence of GB14 and Biocement D specimens cell proliferation was reduced and cell differentiation increased. At day 21, the greatest number of cells was found on GB9 expressing significantly higher levels of bone-related proteins than cells grown on all other surfaces. Because all novel materials facilitated the expression of the osteoblastic phenotype at least as much as TCP and the polystyrene control, these biomaterials can be regarded as excellent candidate bone substitute materials. GB9 induced the highest proliferation and cellular differentiation after 21 days of incubation, suggesting that this material may possess a higher potency for enhancing osteogenesis than TCP. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 145-154, 2004 KW - Calcium phosphate ceramics KW - Human bone-derived cells KW - Cell-biomaterial interactions KW - Calcium phosphate cements KW - In situ hybridization PY - 2004 DO - https://doi.org/10.1002/jbm.a.20131 SN - 1549-3296 SN - 1552-4965 VL - 69 IS - 1 SP - 145 EP - 154 PB - Wiley CY - Hoboken, NJ AN - OPUS4-3502 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Klar, F. A1 - Zreiqat, H. T1 - The Effect of Different Dental Titanium and Calcium Phosphate Implant Surfaces on the Expression of Bone-Related Genes and Proteins in vitro T2 - Transactions of the Sixth World Biomaterials Congress T2 - 6th World Biomaterials Congress CY - Kamuela, Big Island, HI, USA DA - 2000-05-15 KW - Dental Implant Surfaces PY - 2000 VL - 1 SP - 238 PB - Society for Biomaterials CY - Minneapolis, Minn. AN - OPUS4-869 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Reif, D. A1 - Zreiqat, H. T1 - The Functional Expression of Human Bone Derived Cells Grown on Rapidly Resorbable Calcium Phosphate Ceramics T2 - Transactions of the Sixth World Biomaterials Congress T2 - 6th World Biomaterials Congress CY - Kamuela, Big Island, HI, USA DA - 2000-05-15 KW - Calcium Phosphate Ceramics PY - 2000 SP - 970 PB - Society for Biomaterials CY - Minneapolis, Minn. AN - OPUS4-870 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knabe, C. A1 - Howlett, C. R. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Zreiqat, H. T1 - The Functional Expression of Osteoblasts Grown on Rapid Resorbable Calcium Phosphates JF - Key engineering materials T2 - 15th International Symposium on Ceramics in Medicine ; 15th Annual meeting of the International Society for Ceramics in Medicine ; 15th Bioceramics CY - Sydney, NSW, Australia DA - 2002-12-04 KW - Guided bone regeneration KW - Calcium alkali orthophosphates KW - Osteoblasts KW - Cell differentiation PY - 2003 SN - 1013-9826 VL - 240-242 SP - 679 EP - 682 PB - Trans Tech Publ. CY - Aedermannsdorf AN - OPUS4-2115 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Zreiqat, H. T1 - The functional expression of human osteoblasts grown on resorbable calcium phosphates JF - Key engineering materials N2 - The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation, since it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates on the expression of bone-related genes and proteins by human bone derived cells (HBDC) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were -TCP, a newly synthesized chemical Ca2KNa(PO4)2 (material denominated R17), two glassy crystalline materials with a crystalline phase Ca2KNa(PO4)2 and in addition a small amorphous portion containing either magnesium potassium phosphate (material denominated GB14) or silica phosphate (material denominated GB9). HBDC was grown on the substrates for 3, 5, 7, 14 and 21 days, counted and probed for various mRNAs and proteins (collagen type I, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrates supported continuous cellular growth for 21 days. At day 21, R17, GB9, TCP and GB14. T2 - 15th International Symposium on Ceramics in Medicine CY - Sydney, NSW, Australia DA - 2002-12-04 PY - 2002 DO - https://doi.org/10.4028/www.scientific.net/KEM.240-242.679 SN - 1013-9826 VL - 240-242 SP - 679 EP - 682 PB - Trans Tech Publ. CY - Aedermannsdorf AN - OPUS4-1891 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Reif, D. A1 - Zreiqat, H. T1 - The functional expression of human bone derived cells grown on resorbable bioactive glass ceramics T2 - 28th annual meeting & exposition T2 - 28th Annual Meeting & Exposition of the Society for Biomaterials CY - Tampa, FL, USA DA - 2002-04-23 PY - 2002 SP - 706 PB - Society for Biomaterials AN - OPUS4-1892 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, C. A1 - Howlett, C.R. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Zreiqat, H. T1 - Beeinflussung der Gen- und Proteinexpression von Osteoblasten durch bioaktive resorbierbare Glaskeramiken T2 - Bericht der 2. gemeinsamen Jahrestagung der Deutschen Gesellschaft für Implantologie im Zahn-, Mund- und Kieferbereich, der Österreichischen Gesellschaft für orale Chirurgie und Implantologie sowie der Schweizerischen Gesellschaft für orale Implantologie T2 - Bericht der 2. gemeinsamen Jahrestagung der Deutschen Gesellschaft für Implantologie im Zahn-, Mund- und Kieferbereich, der Österreichischen Gesellschaft für orale Chirurgie und Implantologie sowie der Schweizerischen Gesellschaft für orale Implantologie CY - München, Deutschland DA - 2002-12-05 PY - 2002 AN - OPUS4-1898 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Zreiqat, H. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Knabe, Ch. T1 - The Functional Expression of Human Bone-Derived Cells Grown on Rapidly Resorbable Calcium Phosphate Ceramics JF - Key engineering materials KW - Calcium phosphate ceramics KW - Human bone-derived cells KW - Cell-biomaterial interactions KW - Bone-substitutes KW - in situ hybridization PY - 2004 UR - http://www.scientific.net/1013-9826/ SN - 1013-9826 VL - 254-256 SP - 1059 EP - 1062 PB - Trans Tech Publ. CY - Aedermannsdorf AN - OPUS4-2750 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Zreiqat, H. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Knabe, C. T1 - The functional expression of human bone-derived cells grown on rapidly resorbable calcium phosphate ceramics T2 - Bioceramics 16; 16th International Symposium on Ceramics in Medicine T2 - Bioceramics 16; 16th International Symposium on Ceramics in Medicine CY - Oporto, Portugal DA - 2003-11-06 PY - 2003 AN - OPUS4-5364 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -