TY - JOUR A1 - Hoesl, Simone A1 - Neumann, B. A1 - Techritz, Sandra A1 - Sauter, G. A1 - Simon, R. A1 - Schlüter, H. A1 - Linscheid, M. W. A1 - Theruing, F. A1 - Müller, Larissa A1 - Jakubowski, Norbert T1 - Internal standardization of LA-ICP-MS immunoimaging via printing of universal metal spiked inks onto tissue sections N2 - Formalin-fixed paraffin-embedded (FFPE) specimen from biopsy materials are a widespread sample format for pathologists and medical researchers. Pathologists are archiving vast numbers of FFPE samples which can be stored for decades. Conventional immunohistochemical staining (IHC) of biomarkers on FFPE tissue sections is one of the most important analytical techniques for cancer diagnosis and pathology in general. However standardization for IHC samples and quality management is tedious and differs significantly from clinic to clinic. Combining established IHC staining strategies with modern mass spectrometry mediated methods would increase it`s potential and enable access of large FFPE archives for multiplexed quantitation purposes. In this work element mass spectrometry and a new ink-jet printed internal standardization approach was successfully combined with IHC staining to facilitate quantitative multiplex assays for archived FFPE samples. The printing strategy improves elemental image resolution and reproducibility of paraffin embedded breast cancer tissue sections in laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using conventional IHC staining as a model system to investigate the new capabilities of this technique. For the internal standardization we applied a conventional CD-ink-jet printer to print a metal spiked ink onto the top of thin layer tissue sections with constant density. Printing was carried out in a direct comparison to an iodination of the tissue section as previously described as an alternative standardization method. The use of the printed internal standard allowed correction of the fluctuation during the laser ablation process and compensated instrumental drift effects. Mediated by the ink correction approach we achieved better signal-to-background-ratios (SBR) of 74 and better spatial resolution of 30 µm compared to iodination (SBR=23). This improved performance was demonstrated on tumorous areas in FFPE breast cancer tissue sections and allowing detection of Her-2 in tumorous areas of this tissue with significantly improved contrast. KW - Internal standardization KW - LA-ICP-MS KW - Immuno imaging PY - 2016 DO - https://doi.org/10.1039/c5ja00409h SN - 0267-9477 SN - 1364-5544 VL - 31 IS - 3 SP - 801 EP - 808 AN - OPUS4-35711 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schlüter, H. T1 - Dilatometer für große Temperaturspannen PY - 1937 IS - 21 SP - 325 PB - Springer CY - Berlin AN - OPUS4-16921 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schlüter, H. T1 - Über die Berechnung der Fadenberichtigung für geeichte Thermometer PY - 1915 VL - 33 IS - 5-6 SP - 305 EP - 325 PB - Springer CY - Berlin AN - OPUS4-14146 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schlüter, H. T1 - Über die Berechnung der Fadenberichtigung für geeichte Thermometer PY - 1915 VL - 33 IS - 5-6 SP - 305 EP - 325 PB - Springer CY - Berlin AN - OPUS4-14134 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schlüter, H. T1 - Vereinheitlichung der Versuchsverfahren für Ölprüfungen PY - 1926 VL - Neue Folge IS - 3 SP - 69 EP - 71 PB - Springer CY - Berlin AN - OPUS4-15311 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schlüter, H. T1 - Vereinheitlichung der Ölprüfungsverfahren PY - 1929 IS - 6 SP - 79 EP - 87 PB - Springer CY - Berlin AN - OPUS4-15600 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Habel, Wolfgang A1 - Baumann, I. A1 - Berghmans, F. A1 - Borzycki, K. A1 - Chojetzki, C. A1 - Haase, K.-H. A1 - Jaroszewicz, L.R. A1 - Kleckers, T. A1 - Niklès, M. A1 - Rothhardt, M. A1 - Schlüter, Vivien A1 - Thévenaz, L. A1 - Tur, M. A1 - Wuilpart, M. T1 - Guidelines for the characterization and use of fibre optic sensors - Basic definitions & a proposed standard for FBG-based strain sensors N2 - This paper describes the outcome of two groups which are involved in the specification of guidelines for fibre optic sensors performance and testing. The "Guideline for use of fibre optic sensors" from the COST-299 guideline group, and the "Optical Strain Sensor based on Fibre Bragg Grating" from the GESA guideline group of the VDI - "The Association of German Engineers". Through appropriate specifications and definitions, both guidelines aim at enabling better understanding of fibre optic sensors characteristics and performances. A concise view into the structure of the guidelines is presented, emphasizing important aspects. The English version of the two guidelines will be available in autumn 2009. KW - Guideline KW - Standards KW - Fibre optic sensor KW - Strain sensor KW - Fibre Bragg grating KW - Distributed sensor PY - 2009 DO - https://doi.org/10.1117/12.835362 SN - 0038-7355 SN - 0361-0748 SN - 0277-786X VL - 7503 SP - 75035E-1 - 75035E-4 PB - SPIE, The International Society for Optical Engineering CY - Billingham, Wash. AN - OPUS4-20110 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -