TY - CONF A1 - Drescher, Daniela T1 - Combining SERS and viablility tests for the investigation of nanotoxicity T2 - ESCBM - 14th European Conference on the Spectroscopy of Biological Molecules CY - Coimbra, Portugal DA - 2011-08-29 PY - 2011 AN - OPUS4-24186 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Drescher, Daniela T1 - Silver nanoparticles in cultured cells as viewed from a combined SERS, bioassy, and ultrastructural approach T2 - CellNanoSpec 2011 CY - Porquerolles, France DA - 2011-09-11 PY - 2011 AN - OPUS4-24187 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Drescher, Daniela T1 - Combination of SERS and advanced microscopic methods for the investigation of protein-particle-cell interactions T2 - Workshop "FT-IR Spectroscopy in Microbiological and Medical Diagnostics" CY - Berlin, Germany DA - 2011-10-20 PY - 2011 AN - OPUS4-24888 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, Daniela A1 - Kneipp, Janina T1 - Nanomaterials in complex biological systems: insights from Raman spectroscopy N2 - The interaction of nanomaterials with biomolecules, cells, and organisms plays an important role in cell biology, toxicology, and nanotechnology. Spontaneous Raman scattering can be used to probe biomolecules, cells, whole animals, and nanomaterials alike, opening interesting avenues to study the interaction of nanoparticles with complex biological systems. In this review we discuss work in biomedical Raman spectroscopy that has either been concerned directly with nanostructures and biosystems, or that indicates important directions for successful future studies on processes associated with nano-bio-interactions. KW - Cells KW - Nanomaterials KW - Raman PY - 2012 DO - https://doi.org/10.1039/c2cs35127g SN - 0306-0012 SN - 1460-4744 VL - 41 IS - 17 SP - 5780 EP - 5799 PB - Royal Society of Chemistry CY - London AN - OPUS4-26184 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hornemann, A. A1 - Drescher, Daniela A1 - Flemig, Sabine A1 - Kneipp, Janina T1 - Intracellular SERS hybrid probes using BSA-reporter conjugates N2 - Surface-enhanced Raman scattering (SERS) hybrid probes are characterized by the typical spectrum of a reporter molecule. In addition, they deliver information from their biological environment. Here, we report SERS hybrid probes generated by conjugating different reporter molecules to bovine serum albumin (BSA) and using gold nanoparticles as plasmonic core. Advantages of the BSA-conjugate hybrid nanoprobes over other SERS nanoprobes are a high biocompatibility, stabilization of the gold nanoparticles in the biological environment, stable reporter signals, and easy preparation. The coupling efficiencies of the BSA–reporter conjugates were determined by MALDI-TOF-MS. The conjugates' characteristic SERS spectra differ from the spectra of unbound reporter molecules. This is a consequence of the covalent coupling, which leads to altered SERS enhancement and changes in the chemical structures of the reporter and of BSA. The application of the BSA–reporter conjugate hybrid probes in 3T3 cells, including duplex imaging, is demonstrated. Hierarchical cluster analysis and principal components analysis were applied for multivariate imaging using the SERS signatures of the incorporated SERS hybrid nanoprobes along with the spectral information from biomolecules in endosomal structures of cells. The results suggest more successful applications of the SERS hybrid probes in cellular imaging and other unordered high-density bioanalytical sensing. KW - Bovine serum albumin KW - Nanosensor KW - SERS multiplexing KW - 3T3 cells KW - Gold nanoparticles KW - Hybrid nanoprobe KW - Rinderserumalbumin KW - Albumin KW - SERS KW - Konjugate KW - Conjugates KW - Cell KW - Zelle PY - 2013 DO - https://doi.org/10.1007/s00216-013-7054-3 SN - 1618-2642 SN - 1618-2650 VL - 405 IS - 19 SP - 6209 EP - 6222 PB - Springer CY - Berlin AN - OPUS4-29819 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Drescher, Daniela T1 - Investigation of nanoparticle-cell interactions by combining SERS with laser ablation ICP-MS and 3D X-ray tomography T2 - 9th Workshop FT-IR Spectroscopy in Microbiological and Medical Diagnostics CY - Berlin, Germany DA - 2013-10-24 PY - 2013 AN - OPUS4-29378 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hoffmann, Katrin A1 - Behnke, Thomas A1 - Drescher, Daniela A1 - Kneipp, Janina A1 - Resch-Genger, Ute T1 - Lifetime-based discrimination between spectrally matching vis and NIR emitting particle labels and probes N2 - Increasing the information content from bioassays which requires robust and efficient strategies for the detection of multiple analytes or targets in a single measurement is an important field of research, especially in the context of meeting current security and health concerns. An attractive alternative to spectral multiplexing, which relies on fluorescent labels excitable at the same wavelength, yet sufficiently differing in their emission spectra or color presents lifetime multiplexing. For this purpose, we recently introduced a new strategy based on 'pattern-matching' in the lifetime domain, which was exemplary exploited for the discrimination between organic dyes and quantum dot labels revealing multi-exponential decay kinetics and allowed quantification of these labels. Meanwhile, we have succeeded in extending this lifetime multiplexing approach to nanometer-sized particle labels and probes absorbing and emitting in the visible (vis) and near-infrared (NIR) spectral region. Here, we present a first proof-of-principle of this approach for a pair of NIR-fluorescent particles. Each particle is loaded with a single organic dye chosen to display very similar absorption and emission spectra, yet different fluorescence decay kinetics. Examples for the lifetime-based distinction between pairs of these fluorescent nanoparticles in solution and in cells are presented. The results underline the potential of fluorescenc lifetime multiplexing in life science and bioanalysis. KW - Fluorescence KW - Fluorescence lifetime imaging microscopy KW - FLIM KW - Lifetime Multiplexing KW - Particle Label KW - Near-infrared KW - NIR KW - Cell imaging KW - Nanoparticles PY - 2011 DO - https://doi.org/10.1117/12.881442 SN - 1605-7422 VL - 7905 SP - 79051F-1 EP - 79051F-9 PB - SPIE, The International Society for Optical Engineering CY - Bellingham, Wash. AN - OPUS4-23637 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, Daniela A1 - Zeise, Ingrid A1 - Traub, Heike A1 - Guttmann, P. A1 - Seifert, Stephan A1 - Büchner, Tina A1 - Jakubowski, Norbert A1 - Schneider, G. A1 - Kneipp, Janina T1 - In situ characterization of SiO2 nanoparticle biointeractions using BrightSilica N2 - By adding a gold core to silica nanoparticles (BrightSilica), silica-like nanoparticles are generated that, unlike unmodified silica nanoparticles, provide three types of complementary information to investigate the silica nano-biointeraction inside eukaryotic cells in situ. Firstly, organic molecules in proximity of and penetrating into the silica shell in live cells are monitored by surface-enhanced Raman scattering (SERS). The SERS data show interaction of the hybrid silica particles with tyrosine, cysteine and phenylalanine side chains of adsorbed proteins. Composition of the biomolecular corona of BrightSilica nanoparticles differs in fibroblast and macrophage cells. Secondly, quantification of the BrightSilica nanoparticles using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping indicates a different interaction of silica nanoparticles compared to gold nanoparticles under the same experimental conditions. Thirdly, the metal cores allow the investigation of particle distribution and interaction in the cellular ultrastructure by cryo nanoscale X-ray tomography (cryo-XT). In 3D reconstructions the assumption is confirmed that BrightSilica nanoparticles enter cells by an endocytotic mechanism. The high SERS intensities are explained by the beneficial plasmonic properties due to agglomeration of BrightSilica. The results have implications for the development of multi-modal qualitative and quantitative characterization in comparative nanotoxicology and bionanotechnology. KW - Silica nanoparticles KW - Surface-enhanced Raman scattering KW - X-ray tomography KW - LA-ICP-MS KW - Core–shell structures PY - 2014 DO - https://doi.org/10.1002/adfm.201304126 SN - 1616-301X SN - 1616-3028 VL - 24 IS - 24 SP - 3765 EP - 3775 PB - Wiley-VCH CY - Weinheim AN - OPUS4-30924 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Traub, Heike A1 - Jakubowski, Norbert A1 - Drescher, Daniela A1 - Baranov, Vladimir A1 - Kneipp, Janina T1 - Trends in single-cell analysis by use of ICP-MS N2 - The analysis of single cells is a growing research field in many disciplines such as toxicology, medical diagnosis, drug and cancer research or metallomics, and different methods based on microscopic, mass spectrometric, and spectroscopic techniques are under investigation. This review focuses on the most recent trends in which inductively coupled plasma mass spectrometry (ICP-MS) and ICP optical emission spectrometry (ICP-OES) are applied for single-cell analysis using metal atoms being intrinsically present in cells, taken up by cells (e.g., nanoparticles), or which are artificially bound to a cell. For the latter, especially element tagged antibodies are of high interest and are discussed in the review. The application of different sample introduction systems for liquid analysis (pneumatic nebulization, droplet generation) and elemental imaging by laser ablation ICP-MS (LA-ICP-MS) of single cells are highlighted. Because of the high complexity of biological systems and for a better understanding of processes and dynamics of biologically or medically relevant cells, the authors discuss the idea of 'multimodal spectroscopies.' KW - Bioanalytical methods KW - Cell systems/single cell analysis KW - Mass spectrometry/ICP-MS PY - 2014 DO - https://doi.org/10.1007/s00216-014-8143-7 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 6963 EP - 6977 PB - Springer CY - Berlin AN - OPUS4-31717 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Büchner, Tina A1 - Drescher, Daniela A1 - Traub, Heike A1 - Schrade, P. A1 - Bachmann, S. A1 - Jakubowski, Norbert A1 - Kneipp, Janina T1 - Relating surface-enhanced Raman scattering signals of cells to gold nanoparticle aggregation as determined by LA-ICP-MS micromapping N2 - The cellular response to nanoparticle exposure is essential in various contexts, especially in nanotoxicity and nanomedicine. Here, 14-nm gold nanoparticles in 3T3 fibroblast cells are investigated in a series of pulse-chase experiments with a 30-min incubation pulse and chase times ranging from 15 min to 48 h. The gold nanoparticles and their aggregates are quantified inside the cellular ultrastructure by laser ablation inductively coupled plasma mass spectrometry micromapping and evaluated regarding the surface-enhanced Raman scattering (SERS) signals. In this way, both information about their localization at the micrometre scale and their molecular nanoenvironment, respectively, is obtained and can be related. Thus, the nanoparticle pathway from endocytotic uptake, intracellular processing, to cell division can be followed. It is shown that the ability of the intracellular nanoparticles and their accumulations and aggregates to support high SERS signals is neither directly related to nanoparticle amount nor to high local nanoparticle densities. The SERS data indicate that aggregate geometry and interparticle distances in the cell must change in the course of endosomal maturation and play a critical role for a specific gold nanoparticle type in order to act as efficient SERS nanoprobe. This finding is supported by TEM images, showing only a minor portion of aggregates that present small interparticle spacing. The SERS spectra obtained after different chase times show a changing composition and/or structure of the biomolecule corona of the gold nanoparticles as a consequence of endosomal processing. KW - Gold nanoparticles KW - Surface-enhanced Raman scattering KW - LA-ICP-MS KW - Fibroblast KW - Cell KW - Particle aggregation KW - Endosome PY - 2014 DO - https://doi.org/10.1007/s00216-014-8069-0 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 7003 EP - 7014 PB - Springer CY - Berlin AN - OPUS4-31718 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, Daniela A1 - Giesen, Charlotte A1 - Traub, Heike A1 - Panne, Ulrich A1 - Kneipp, Janina A1 - Jakubowski, Norbert T1 - Quantitative imaging of gold and silver nanoparticles in single eukaryotic cells by laser ablation ICP-MS N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized for spatially resolved bioimaging of the distribution of silver and gold nanoparticles in individual fibroblast cells upon different incubation experiments. High spatial resolution was achieved by optimization of scan speed, ablation frequency, and laser energy. Nanoparticles are visualized with respect to cellular substructures and are found to accumulate in the perinuclear region with increasing incubation time. On the basis of matrix-matched calibration, we developed a method for quantification of the number of metal nanoparticles at the single-cell level. The results provide insight into nanoparticle/cell interactions and have implications for the development of analytical methods in tissue diagnostics and therapeutics. KW - Imaging KW - Cell KW - Nanoparticles KW - Laser ablation KW - ICP-MS PY - 2012 DO - https://doi.org/10.1021/ac302639c SN - 0003-2700 SN - 1520-6882 VL - 84 IS - 22 SP - 9684 EP - 9688 PB - American Chemical Society CY - Washington, DC AN - OPUS4-27440 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Giesen, Charlotte A1 - Müller, Larissa A1 - Mairinger, T. A1 - Drescher, Daniela A1 - Kneipp, Janina A1 - Roos, P.H. A1 - Panne, Ulrich A1 - Jakubowski, Norbert T1 - Iodine as an elemental marker for imaging of single cells and tissue sections by laser ablation inductively coupled plasma mass spectrometry N2 - A new laser ablation (LA)-ICP-MS method for single cell and cell nucleus imaging was developed. Therein, iodine was employed as an elemental dye for fibroblast cells and for thin tissue sections. At an incubation time of 60 s, iodine is located mainly within the cell nuclei. This effect was illustrated in fibroblast cells, and iodine signal within the cell nucleus was as high as 5 × 104 cps at 4 µm laser spot size. The surrounding cytoplasm was iodinated as well, but to a lesser extent. The spatial resolution attained was sufficient to detect even smaller cell nuclei within a liver biopsy tissue. Furthermore, iodine was successfully employed for biomolecule labeling and we demonstrated that iodine signal increased with increasing thickness of a palatine tonsil tissue. Thus, the use of iodine as an internal standard to correct for tissue inhomogeneities in LA-ICP-MS was investigated for the simultaneous detection of two tumor markers (Her 2 and CK 7) in breast cancer tissue. Additionally, lanthanide background resulting from glass ablation can be corrected for by Eu standardization. PY - 2011 DO - https://doi.org/10.1039/c1ja10227c SN - 0267-9477 SN - 1364-5544 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. VL - 26 IS - 11 SP - 2160 EP - 2165 PB - Royal Society of Chemistry CY - London AN - OPUS4-24964 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hoffmann, Katrin A1 - Behnke, Thomas A1 - Drescher, Daniela A1 - Kneipp, Janina A1 - Resch-Genger, Ute T1 - Near-infrared-emitting nanoparticles for lifetime-based multiplexed analysis and imaging of living cells N2 - The increase in information content from bioassays and bioimaging requires robust and efficient strategies for the detection of multiple analytes or targets in a single measurement, thereby addressing current health and security concerns. For fluorescence techniques, an attractive alternative to commonly performed spectral or color multiplexing presents lifetime multiplexing and the discrimination between different fluorophores based on their fluorescence decay kinetics. This strategy relies on fluorescent labels with sufficiently different lifetimes that are excitable at the same wavelength and detectable within the same spectral window. Here, we report on lifetime multiplexing and discrimination with a set of nanometer-sized particles loaded with near-infrared emissive organic fluorophores chosen to display very similar absorption and emission spectra, yet different fluorescence decay kinetics in suspension. Furthermore, as a first proof-of-concept, we describe bioimaging studies with 3T3 fibroblasts and J774 macrophages, incubated with mixtures of these reporters employing fluorescence lifetime imaging microscopy. These proof-of-concept measurements underline the potential of fluorescent nanoparticle reporters in fluorescence lifetime multiplexing, barcoding, and imaging for cellular studies, cell-based assays, and molecular imaging. KW - Fluorescence lifetime imaging microscopy KW - FLIM KW - Lifetime multiplexing KW - Near infrared KW - NIR KW - Cell imaging KW - Nanoparticles PY - 2013 DO - https://doi.org/10.1021/nn4029458 SN - 1936-0851 VL - 7 IS - 8 SP - 6674 EP - 6684 PB - ACS Publ. CY - Washington, DC, USA AN - OPUS4-29031 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -