TY - CONF A1 - Kage, Daniel A1 - Hoffmann, Katrin A1 - Göhde, W. A1 - Thiele, T. A1 - Schedler, U. A1 - Resch-Genger, Ute T1 - Lifetime-encoded microparticles - application to flow cytometry N2 - Analytical methods require efficient and versatile strategies to measure an increasing number of analytes that can be used in conjunction with established platforms like flow cytometry. Spectral multiplexing suffers from problems such as spectral crosstalk and often requires different excitation light sources increasing instrumentation costs. Thus, the number of distinguishable reporters with intensity-based barcodes is limited. An alternative can be lifetime encoding for discrimination of fluorophores based on their fluorescence decay kinetics. We report on the suitability of µm-sized polymer particles stained with organic dyes for lifetime encoding. These dyes are excitable at a standard laser diode wavelength and detectable within a single spectral window. For lifetime-based discrimination, these dyes display sufficiently different luminescence decay kinetics. We present the spectroscopic properties of these beads and address challenges like the limited number of detectable photons in a flow for the reliable discrimination. These studies are expected to pave the road to new applications of fluorescence lifetime multiplexing for time-domain flow cytometry. T2 - DPG Spring Meeting 2016 CY - Regensburg, Germany DA - 06.03.2016 KW - flow cytometry KW - lifetime encoding KW - life sciences KW - fluorescence KW - polymer particles PY - 2016 AN - OPUS4-37153 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kage, Daniel A1 - Hoffmann, Katrin A1 - Wittkamp, M. A1 - Ameskamp, J. A1 - Göhde, W. A1 - Thiele, T. A1 - Schedler, U. A1 - Resch-Genger, Ute T1 - Lifetime-encoded microparticles for application in flow cytometry N2 - The steadily increasing number of analytes, especially in bioanalytics and environmental contexts, requires the development of efficient and versatile methods for the simultaneous determination of different parameters within a single run. Additionally, these methods should be applicable in conjunction with established platform technologies like flow cytometry. Fluorescence techniques have proven to fulfill these requirements. Commonly performed spectral multiplexing utilizing a color code suffers from several problems, such as the sensitivity of fluorescence intensity measurements to fluctuations in excitation light intensity and dye concentration and hence, photobleaching and spectral crosstalk limiting the achievable number of detection channels. Moreover, it typically requires different costly excitation light sources. T2 - Innovation Forum Senftenberg 2016 CY - Senftenberg, Germany DA - 01.06.2016 KW - flow cytometry KW - lifetime encoding KW - life sciences KW - fluorescence KW - polymer particles PY - 2016 AN - OPUS4-37154 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -