TY - JOUR A1 - Weiss, S. I. A1 - Sieverling, N. A1 - Niclasen, M. A1 - Maucksch, Ch. A1 - Thünemann, Andreas A1 - Möhwald, H. A1 - Reinhardt, D. A1 - Rosenecker, J. A1 - Rudolph, C. T1 - Uronic acids functionalized polyethyleneimine (PEI)-polyethyleneglycol (PEG)-graft-copolymers as novel synthetic gene carriers N2 - In this study, we investigated galacturonic (GalAc)- and mannuronic (ManAc) acids as novel targeting ligands for receptor-mediated gene delivery. GalAc and ManAc were coupled to either polyethyleneimine (PEI) or PEI–polyethyleneglycol (PEG). Furthermore, lactobionic acid (LacAc), which comprises a GalAc-related carbohydrate ring, was coupled to each of the polymers through its open-chain gluconic acid moiety. The molar mass distributions of the polymers were characterized by analytical ultracentrifugation and size exclusion chromatography. PEI-conjugate–pDNA complexes were transfected into HepG2-, HeLa-, and 16HBE14o--cells. Gene expression mediated by GalAc- and LacAc-functionalized PEI-conjugates was lower than for PEI. In contrast, gene expression mediated by ManAc-functionalized PEI-conjugates was up to three orders of magnitude higher than for the other tested PEI-conjugates, in particular for negatively charged gene vectors at low N/P ratios, independent of the cell line. Pre-incubation of cells with an excess of ManAc before transfection significantly inhibited transfection rates only for ManAc-functionalized PEI-conjugates. Coupling of methyl-α-D-mannuronic acid to PEI resulted in significantly lower transfection rates than for ManAc-PEI based complexes. Together with fluorescence microscopy images of fluorescein-labelled ManAc-functionalized dextrans and FACS analyses of cells, these results demonstrate that receptor-mediated endocytosis of ManAc–PEI-conjugate–pDNA complexes via ManAc-specific receptors was involved in gene transfer. In conclusion, ManAc-modification of PEI-polymers represents a novel strategy for receptor-mediated gene delivery which could be promising for in vivo application. KW - Gene therapy KW - Gene transfer KW - Polyethylene oxide KW - Nanoparticle PY - 2006 U6 - https://doi.org/10.1016/j.biomaterials.2005.11.011 SN - 0142-9612 VL - 27 IS - 10 SP - 2302 EP - 2312 PB - Elsevier CY - Oxford AN - OPUS4-11678 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pittner, Andreas A1 - Weiß, D. A1 - Schwenk, Christopher A1 - Rethmeier, Michael T1 - Fast temperature field generation for welding simulation and reduction of experimental effort N2 - The quality of welding processes is governed by the occurring induced distortions yielding an increase in production costs due to necessary reworking. Especially for more complex specimens, it is difficult to evaluate the optimal configuration of welding sequences in order to minimize the distortion. Even experienced welding operators can solve this task only by trial and error which is time and cost consuming. In modern engineering the application of welding simulation is already known to be able to analyse the heat effects of welding virtually. However, the welding process is governed by complex physical interactions. Thus, recent weld thermal models are based on many simplifications. The state of the art is to apply numerical methods in order to solve the transient heat conduction equation. Therefore, it is not possible to use the real process parameters as input for the mathematical model. The model parameters which allow calculating a temperature field that is in best agreement with the experiments cannot be defined directly but inversely by multiple simulations runs. In case of numerical simulation software based on finite discretization schemes this approach is very time consuming and requires expert users. The weld thermal model contains an initial weakness which has to be adapted by finding an optimal set of model parameters. This process of calibration is often done against few experiments. The range of model validity is limited. An extension can be obtained by performing a calibration against multiple experiments. The focus of the paper is to show a combined modelling technique which provides an efficient solution of the inverse heat conduction problem mentioned above. On the one hand the inverse problem is solved by application of fast weld thermal models which are closed form solutions of the heat conduction equation. In addition, a global optimization algorithm allows an automated calibration of the weld thermal model. This technique is able to provide a temperature field automatically that fits the experimental one with high accuracy within minutes on ordinary office computers. This fast paradigm permits confirming the application of welding simulation in an industrial environment as automotive industry. On the other hand, the initial model weakness is compensated by calibrating the model against multiple experiments. The unknown relationship between model and process parameters is approximated by a neural network. The validity of the model is increased successively and enables to decrease experimental effort, For a test case, it is shown that this approach yields accurate temperature fields within very short amount of time for unknown process parameters as input data to the model contributing to the requirement to construct a substitute system of the real welding process. KW - Heat flow KW - Neural networks KW - Simulating KW - Temperature KW - Welding PY - 2011 SN - 0043-2288 SN - 1878-6669 VL - 55 IS - 09-10 SP - 83 EP - 90 PB - Springer CY - Oxford AN - OPUS4-24603 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pittner, Andreas A1 - Weiß, D. A1 - Schwenk, Christopher A1 - Rethmeier, Michael T1 - Methodology to improve applicability of welding simulation N2 - The objective of this paper is to demonstrate a new simulation technique which allows fast and automatic generation of temperature fields as input for subsequent thermomechanical welding simulation. The basic idea is to decompose the process model into an empirical part based on neural networks and a phenomenological part that describes the physical phenomena. The strength of this composite modelling approach is the automatic calibration of mathematical models against experimental data without the need for manual interference by an experienced user. As an example for typical applications in laser beam and GMA-laser hybrid welding, it is shown that even 3D heat conduction models of a low complexity can approximate measured temperature fields with a sufficient accuracy. In general, any derivation of model fitting parameters from the real process adds uncertainties to the simulation independent of the complexity of the underlying phenomenological model. The modelling technique presented hybridises empirical and phenomenological models. It reduces the model uncertainties by exploiting additional information which keeps normally hidden in the data measured when the model calibration is performed against few experimental data sets. In contrast, here the optimal model parameter set corresponding to a given process parameter is computed by means of an empirical submodel based on relatively large set of experimental data. The approach allows making a contribution to an efficient compensation of modelling inaccuracies and lack of knowledge about thermophysical material properties or boundary conditions. Two illustrating examples are provided. KW - Welding simulation KW - GMA-laser hybrid welding KW - Laser beam welding KW - Neural networks KW - Global optimisation KW - Stochastic search method KW - Inverse heat conduction problem KW - Model prediction PY - 2008 U6 - https://doi.org/10.1179/136217108X329322 SN - 1362-1718 SN - 1743-2936 VL - 13 IS - 6 SP - 496 EP - 508 PB - Maney CY - London AN - OPUS4-18300 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Pittner, Andreas A1 - Weiss, D. A1 - Schwenk, Christopher A1 - Rethmeier, Michael T1 - A methodology for the fast temperature field generation for welding simulation T2 - 17th International Conference "Computer Technology in Welding and Manufacturing" CY - Cranfield, UK DA - 2008-06-18 KW - Welding simulation KW - Temperature field generation KW - Short calculation time KW - Multiple experiments KW - Inverse heat conduction problem KW - Neural networks PY - 2008 SN - 978-1-903761-07-6 SP - 1 EP - 12 PB - TWI CY - Cambridge AN - OPUS4-18290 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Schwenk, Christopher A1 - Rethmeier, Michael A1 - Weiss, D. ED - H.K.D.H. Bhadeshia, ED - H. Cerjak, ED - E. Kozeschnik, T1 - Rapid generation of temperature fields for simulation of welding distortions PY - 2007 SN - 978-3-902465-69-6 SP - 835 EP - 846 CY - Graz, Austria AN - OPUS4-16491 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pittner, Andreas A1 - Weiss, D. A1 - Schwenk, Christopher A1 - Rethmeier, Michael ED - V.I. Makhnenko, T1 - Fast generation and prediction of welding temperature fields for multiple experiments N2 - The objective of this paper is to demonstrate a new simulation technique which allows the fast and automatic generation to temperature fields based on a combination of empirical and phenomenological modelling techniques. The automatic calibration of the phenomenological model is performed by a multi-variable global optimisation routine which yields the optimal fit between simulated and experimental weld charcteristics without the need for initial model parameters. For exemplary welding processes it is shown that linear 3D heat conduction models can approximate measured temperature fields with a high accuracy. The modelling approach presented comprises the automatic calibration against multiple experiments which permits simulating the temperature field for unknown process parameters. The validation of this composite simulation model is performed for exemplary welding processes and includes the prediction of the fusion line in the cross section and the corresponding thermal cycles. T2 - 4th International Conference - Mathematical modelling and information technologies in welding and related processes CY - Katsiveli, Crimea, Ukraine DA - 2008-05-27 KW - Welding simulation KW - GMA-laser hybrid welding KW - Laser beam welding KW - Neural networks KW - Global optimisation KW - Stochastic search method KW - Inverse heat conduction problem KW - Model prediction PY - 2008 SP - 134 EP - 140 CY - Kiev, Ukraine AN - OPUS4-19639 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Pittner, Andreas A1 - Weiss, D. A1 - Schwenk, Christopher A1 - Rethmeier, Michael T1 - Fast temperature field generation for welding simulation and reduction of experimental effort N2 - The quality of welding processes is governed by the occurring induced distortions yielding an increase in production costs due to necessary reworking. Especially for more complex specimens it is difficult to evaluate the optimal configuration of welding sequences in order to minimise the distortion. Even experienced welding operators can solve this task only by trial and error which is time and cost consuming. In modern engineering the application of welding simulation is already known to be able to analyse the heat effects of welding virtually. However, the welding process is governed by complex physical interactions. Thus, recent weld thermal models are based on many simplifications. The state of the art is to apply numerical methods in order to solve the transient heat conduction equation. Therefore, it is not possible to use the real process parameters as input for the mathematical model. The model parameters which allow calculating a temperature field that is in best agreement with the experiments cannot be defined directly but inversely by multiple simulations runs. In case of numerical simulation software based on finite discretisation schemes this approach is very time consuming and requires expert users. The weld thermal model contains an initial weakness which has to be adapted by finding an optimal set of model parameters. This process of calibration is often done against few experiments. The range of model validity is limited. An extension can be obtained by performing a calibration against multiple experiments. The focus of the paper is to show a combined modelling technique which provides an efficient solution of the inverse heat conduction problem mentioned above. On the one hand the inverse problem is solved by application of fast weld thermal models which are closed form solutions of the heat conduction equation. In addition, a global optimisation algorithm allows an automated calibration of the weld thermal model. This technique is able to provide a temperature field automatically that fits the experimental one with high accuracy within minutes on ordinary office computers. This fast paradigm permits confirming the application of welding simulation in an industrial environment as automotive industry. On the other hand, the initial model weakness is compensated by calibrating the model against multiple experiments. The unknown relationship between model and process parameters is approximated by a neural network. The validity of the model is increased successively and enables to decrease experimental effort. For a test case it is shown, that this approach yields accurate temperature fields within very short amount of time for unknown process parameters as input data to the model contributing to the requirement to construct a substitute system of the real welding process. KW - Welding simulation KW - Temperature field generation KW - Short calculation time KW - Multiple experiments KW - Inverse heat conduction problem KW - Neural networks PY - 2009 IS - SC-Auto-32-09 SP - 1 EP - 11 PB - International Institute of Welding CY - Paris AN - OPUS4-19744 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pittner, Andreas A1 - Schwenk, Christopher A1 - Weiß, D. A1 - Rethmeier, Michael T1 - Application of a combined modelling technique to reduce experimental effort - a case study for laser-GMA-hybrid welding T2 - VI. International conference "Beam technologies & laser application" CY - Saint Petersburg, Russia DA - 2009-09-23 PY - 2009 SP - 97 EP - 102 CY - Saint Petersburg, Russia AN - OPUS4-20882 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Pittner, Andreas A1 - Schwenk, Christopher A1 - Weiß, D. A1 - Rethmeier, Michael ED - Cerjak, H. ED - Enzinger, N. T1 - An efficient solution of the inverse heat conduction problem for welding simulation PY - 2010 SN - 978-3-85125-127-2 SP - 761 EP - 791 PB - Verlag der Technischen Universität Graz AN - OPUS4-23154 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Reuter, T. A1 - Plotzki, David A1 - Borges de Oliveira, F. A1 - Wohlgemuth, F. A1 - Bauer, F. A1 - Ballach, F. A1 - Kimmig, W. A1 - Wagner, G. A1 - Watzl, C. A1 - Weiß, D. A1 - Hausotte, T. T1 - Numerical measurement uncertainty evaluation for X-ray computed tomography using simulations – A first approach to build a digital twin N2 - The ability of industrial X-ray computed tomography (CT) to scan an object with several internal and external features at once causes increasing adoption in dimensional metrology. In order to evaluate the quality of a measurement value, the task-specific measurement uncertainty has to be determined. Currently, VDI/VDE 2630 part 2.1 gives a guideline to determine the uncertainty of CT measurements experimentally by conducting repeated measurements. This is costly and time-consuming. Thus, the aim is to determine the task-specific measurement uncertainty numerically by simulations (e. g. according to the guide to expression of uncertainty in measurement (GUM) Supplement 1). To achieve that, a digital twin is necessary. This contribution presents a simple first approach how a digital twin can be built. In order to evaluate this approach, a study comparing measurements and simulations of different real CT systems was carried out by determining the differences between the measurement results of the digital twin and of the measurement results of the real-world CT systems. The results have shown a moderate agreement between real and simulated data. To improve on this aspect, a standardized method to characterize CT systems and methods to implement CT parameters into the simulation with sufficient accuracy will be developed. N2 - Die industrielle Röntgencomputertomografie (CT) wird durch ihre Fähigkeit, ein Messobjekt mit unterschiedlichen inneren und äußeren Merkmalen auf einmal zu messen, immer häufiger auch in der dimensionellen Messtechnik eingesetzt. Um die Güte eines Messwertes beurteilen zu können, muss die aufgabenspezifische Messunsicherheit ermittelt werden. Mit der Richtlinie VDI/VDE 2630 Blatt 2.1 kann derzeit die aufgabenspezifische Messunsicherheit von CT-Messungen experimentell durch eine Vielzahl an Wiederholmessungen ermittelt werden. Diese Vorgehensweise ist allerdings kosten- und zeitintensiv. Daher wird die Bestimmung der aufgabenspezifischen Messunsicherheit numerisch durch Simulationen angestrebt (z. B. Leitfaden zur Angabe der Unsicherheit beim Messen (GUM) Supplement 1). Um dieses Ziel zu erreichen, ist es notwendig, einen Digitalen Zwilling von der Messung mit dem verwendeten CT-System zu erstellen. Dieser Beitrag stellt einen ersten Ansatz zur Erstellung eines Digitalen Zwillings vor. Um diesen anschließend zu testen, wurden in einem ersten Test mehrere CT-Scans auf verschiedenen Anlagen durchgeführt und anschließend anhand der verfügbaren Parameter simuliert. Um die Qualität des jeweiligen Digitalen Zwillings einschätzen zu können, wurden die Abweichungen der resultierenden Messwerte von ihren jeweiligen Sollwerten miteinander verglichen. Die Ergebnisse zeigten eine bedingte Übereinstimmung zwischen realen und simulierten Daten. Um diese zu verbessern, wird eine standardisierte Methode zur Charakterisierung der realen CT-Systeme angestrebt. Darüber hinaus sollten Methoden entwickelt werden, die Charakterisierungsparameter in einer Simulationsumgebung hinreichend genau umzusetzen. KW - Computed tomography KW - Measurement uncertainty KW - Simulation KW - Digital twin PY - 2022 U6 - https://doi.org/10.1515/teme-2022-0025 SP - 1 EP - 16 PB - De Gruyter CY - Berlin AN - OPUS4-55428 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pittner, Andreas A1 - Schwenk, Christopher A1 - Rethmeier, Michael A1 - Weiß, D. T1 - Automated generation of temperature fields for numerical welding simulation KW - Welding simulation KW - Temperature field generation KW - Optimization KW - Neural networks PY - 2009 SN - 0288-4771 VL - 27 IS - 2 SP - 219 EP - 224 CY - Tokyo, Japan AN - OPUS4-19826 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Pittner, Andreas A1 - Schwenk, Christopher A1 - Rethmeier, Michael A1 - Weiß, D. ED - Hirata, Yoshinori ED - Manabu Tanaka, T1 - Automated generation of temperature fields for numerical welding simulation T2 - 8th International Welding Symposium - Innovations in Welding and Joining for a New Era in Manufacturing CY - Kyoto, Japan DA - 2008-11-16 KW - Welding simulation KW - Temperature Field Generation KW - Optimization KW - Neural Networks PY - 2008 SP - 158 PB - Japan Welding Society CY - Kyoto AN - OPUS4-18614 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Osterloh, Kurt A1 - Bellon, Carsten A1 - Hohendorf, Stefan A1 - Kolkoori, Sanjeevareddy A1 - Wrobel, Norma A1 - Nusser, A. A1 - Freitag, M. A1 - Bücherl, T. A1 - Bar, D. A1 - Mor, I. A1 - Tamin, N. A1 - Weiss-Babai, R. A1 - Bromberger, B. A1 - Dangendorf, V. A1 - Tittelmeier, K. T1 - Computed tomography with X-rays and fast neutrons for restoration of wooden artwork N2 - The objects of this investigation were sculptures taken from a ca. three hundred years old baroque epitaph of a church in Tonning, a town in Northern Germany. Around 1900 it was found in a disastrous state heavily damaged by wood-worm. At that time, the whole artwork was treated with the tar extract carbolineum as a remedy. Nowadays, this substance has been identified as carcinogenic, and its presence can be perceived by its stench and visually at certain spots on the surface where it has penetrated the covering paint. A gold-painted sculpture of a massive wooden skull was interrogated with X-rays and fast neutrons to investigate the internal distribution of the carbolineum. The X-ray tomography, with its excellent spatial resolution revealed galleries left over from the worm infestation in the outer areas and cracks in the central region. The golden color coating appeared as a thick and dense layer. In comparison the tomography with fast neutrons, though being of lower resolution and yet unresolved artefacts revealed sections of slightly different densities in the bulk of the wood. These differences we attribute to the differences in the distribution of the impregnant in the wood, visible due to its higher hydrogen content making it less transparent for neutrons. T2 - WCNR-10 - 10th World conference on neutron radiography CY - Grindelwald, Switzerland DA - 05.10.2014 KW - Computed tomography KW - Neutron radiology KW - Conservation of wooden artwork PY - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:b43-323050 SN - 1875-3892 VL - 69 SP - 472 EP - 477 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-32305 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gartiser, S. A1 - Heisterkamp, I. A1 - Schoknecht, Ute A1 - Burkhardt, M. A1 - Ratte, M. A1 - Ilvonen, O. A1 - Brauer, F. A1 - Brückmann, J. A1 - Dabrunz, A. A1 - Egeler, P. A1 - Eisl, A.-M. A1 - Feiler, U. A1 - Fritz, I. A1 - König, S. A1 - Lebertz, H. A1 - Pandard, P. A1 - Pötschke, G. A1 - Scheerbaum, D. A1 - Schreiber, F. A1 - Soldán, P. A1 - Weiß, R. A1 - Weltens, R. T1 - Results from a round robin test for the ecotoxicological evaluation of construction products using two leaching tests and an aquatic test battery N2 - A European round robin test according to ISO 5725-2 was conceptually prepared, realised, and evaluated. The aim was to determine the inter-laboratory variability of the overall process for the ecotoxicological characterization of construction products in eluates and bioassays. To this end, two construction products BAM-G1 (granulate) and HSR-2 (roof sealing sheet), both made of EPDM polymers (rubber), were selected. The granular construction product was eluted in a one stage batch test, the planar product in the Dynamic Surface Leaching test (DSLT). A total of 17 laboratories from 5 countries participated in the round robin test: Germany (12), Austria (2), Belgium (1), Czech Republic (1) and France (1). A test battery of four standardised ecotoxicity tests with algae, daphnia, luminescent bacteria and zebrafish eggs was used. As toxicity measures, EC50 and LID values were calculated. All tests, except the fish egg test, were basically able to demonstrate toxic effects and the level of toxicity. The reproducibility of test results depended on the test specimens and the test organisms. Generally, the variability of the EC50 or LID values increased with the overall level of toxicity. For the very toxic BAM-G1 eluate a relative high variability of CV ¼ 73%e110% was observed for EC50 in all biotests, while for the less toxic HSR-2 eluate the reproducibility of EC50 varied with sensitivity: it was very good (CV ¼ 9.3%) for the daphnia test with the lowest sensitivity, followed by the algae test (CV ¼ 36.4%). The luminescent bacteria test, being the most sensitive bioassay for HSR-2 Eluate, showed the highest variability (CV ¼ 74.8%). When considering the complex overall process the reproducibility of bioassays with eluates from construction products was acceptable. KW - Round robin test KW - Construction products KW - Leaching tests KW - Eluates KW - Ecotoxicity tests PY - 2017 U6 - https://doi.org/10.1016/j.chemosphere.2017.01.146 SN - 0045-6535 SN - 1879-1298 VL - 175 SP - 138 EP - 146 PB - Elsevier Ltd. AN - OPUS4-39174 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Nilsson, R. H. A1 - Taylor, A. F. S. A1 - Adams, R. I. A1 - Baschien, C. A1 - Bengtsson-Palme, J. A1 - Cangren, P. A1 - Coleine, C. A1 - Iršėnaitė, R. A1 - Martin-Sanchez, Pedro Maria A1 - Meyer, W. A1 - Oh, S.-Y. A1 - Sampaio, J. P. A1 - Seifert, K. A. A1 - Sklenář, F. A1 - Stubbe, D. A1 - Suh, S.-O. A1 - Summerbell, R. A1 - Svantesson, S. A1 - Unterseher, M. A1 - Visagie, C. M. A1 - Weiss, M. A1 - Woudenberg, J. HC. A1 - Wurzbacher, C. A1 - Van den Wyngaert, S. A1 - Yilmaz, N. A1 - Yurkov, A. A1 - Kõljalg, U. A1 - Abarenkov, K. A1 - Daniel, H.-M. A1 - Glassman, S. I. A1 - Hirooka, H. A1 - Irinyi, L. T1 - Taxonomic annotation of public fungal ITS sequences from the built environment – a report from an April 10–11, 2017 workshop (Aberdeen, UK) N2 - Recent DNA-based studies have shown that the built environment is surprisingly rich in fungi. These indoor fungi – whether transient visitors or more persistent residents – may hold clues to the rising levels of human allergies and other medical and building-related health problems observed globally. The taxo¬nomic identity of these fungi is crucial in such pursuits. Molecular identification of the built mycobiome is no trivial undertaking, however, given the large number of unidentified, misidentified, and technically compromised fungal sequences in public sequence databases. In addition, the sequence metadata required to make informed taxonomic decisions – such as country and host/substrate of collection – are often lacking even from reference and ex-type sequences. Here we report on a taxonomic annotation workshop (April 10–11, 2017) organized at the James Hutton Institute/University of Aberdeen (UK) to facilitate reproducible studies of the built mycobiome. The 32 participants went through public fungal ITS bar¬code sequences related to the built mycobiome for taxonomic and nomenclatural correctness, technical quality, and metadata availability. A total of 19,508 changes – including 4,783 name changes, 14,121 metadata annotations, and the removal of 99 technically compromised sequences – were implemented in the UNITE database for molecular identification of fungi (https://unite.ut.ee/) and shared with a range of other databases and downstream resources. Among the genera that saw the largest number of changes were Penicillium, Talaromyces, Cladosporium, Acremonium, and Alternaria, all of them of significant importance in both culture-based and culture-independent surveys of the built environment. KW - Indoor mycobiome KW - Built environment KW - Molecular identification KW - Fungi KW - Taxonomy KW - Systematics KW - Sequence annotation KW - Metadata KW - Open data PY - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:b43-438949 SN - 1314-4049 SN - 1314-4057 VL - 28 SP - 65 EP - 82 PB - Pensoft Publishers CY - Washington, DC AN - OPUS4-43894 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schiebel, J. A1 - Noack, J. A1 - Rödiger, S. A1 - Kammel, A. A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Weise, Matthias A1 - Weiss, R. A1 - Böhm, A. A1 - Nitschke, J. A1 - Elimport, A. A1 - Roggenbuck, D. A1 - Schierack, P. T1 - Analysis of three-dimensional biofilms on different material surfaces N2 - Biofilms cause complications and high costs in both industry and medicine. Of particular interest are bacterial infections of prosthetic materials, which usually cannot be eliminated due to the high antibiotic resistance known for bacteria forming biofilms. The search for new materials and coatings with lower colonization potential and antibacterial activity is of great importance to reduce biofilm formation. However, there is no standardized procedure to examine the colonization characteristics of bacteria in the Biofilm state in situ. Here, we describe an automated epifluorescence microscopy system for the semi-quantitative analysis of three-dimensional (3D) biofilms on various surfaces. To analyze adherent bacteria, three materials (glass, steel and titanium) were incubated with bacteria in a flow chamber system. After fluorescence staining of the bacteria, automated image capturing, quantification of the bacteria, measurement of the colonized area and determination of the 3D biofilm height were carried out by using novel software. Furthermore, the materials were examined for their surface topography using white light scanning interferometry. Titanium compared to glass showed a significantly higher number of adherent bacteria. We argue that this was due to the higher microroughness of titanium. The colonized area was in accordance with the number of adherent bacteria and was also significantly larger on titanium coupons compared to glass. Maximum 3D biofilm height on glass coupons was significantly lower compared to the ones on steel and titanium. This novel method enables the standardized, automated investigation of the colonization with bacteria on different materials. This approach can considerably support the characterization of new material surfaces and their innovative coatings by analyzing the amount of attached Bacteria and thickness of biofilms in situ and eliminates the need of conventional cultivation. KW - Biofilm KW - Bacterial adhesion KW - Biofilm quantification KW - Automated analysis PY - 2020 U6 - https://doi.org/10.1039/D0BM00455C SP - 1 EP - 11 PB - Royal Society of Chemistry AN - OPUS4-50815 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Asna Ashari, Parsa A1 - Weiss, D. A1 - Blind, K. ED - Jakobs, K. T1 - Fuel-cell Vehicles in Relation to Electric and Internal Combustion Engine Vehicles – An Analysis of Technology Relations based on Publications, Patents, Standards N2 - Against the backdrop of the sustainability transition of economies worldwide, decarbonizing road traffic is high on the agenda. This has focused the interest of policymakers and automobile manufacturers on sustainable, zero-emission powertrain technologies. Among these technologies, hydrogen fuel cell (FC) vehicles have a positive climate impact, given that their hydrogen is produced from renewable energy. However, FC vehicles have not yet gained significant market shares. Therefore, based on the technological innovation systems (TIS) approach, this study analyzes how FC vehicles are influenced by EVs and internal combustion engine (ICE) vehicles as their context structures. To operationalize the technology relations between our focal FC-TIS and its context structures, we use the sum of international publications, patents filed at the European Patent Office, and international ISO and IEC standards as indicators for each technology. Our results show that the FC-TIS is dominated by its context structures, especially regarding commercially relevant patents and international standards. Therefore, we conclude that the FC-TIS is in its formative life-cycle phase and identify the need for intensified patenting and standardization in relation to the competing EVs and ICE vehicles. T2 - 27th EURAS Annual Standardisation Conference & 12th International Conference on Standardisation and Innovation in Information Technology (SIIT) - (Responsible) Standardisation for Smart Systems CY - Aachen, Germany DA - 28.06.2023 KW - Fuel cell vehicles KW - Patents KW - Publications KW - Standards KW - Technological innovation system (TIS) KW - Technology interaction PY - 2023 SN - 978-3-95886-491-7 SP - 1 EP - 20 PB - Verlagshaus Mainz CY - Aachen AN - OPUS4-57870 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -