TY - JOUR A1 - Hodoroaba, Vasile-Dan A1 - Akcakayiran, D. A1 - Grigoriev, D. A1 - Shchukin, D.G. T1 - High-resolution SEM with coupled transmission mode and EDX for quick characterization of micro- and nanocapsules for self-healing anti-corrosion coatings JF - Microscopy and microanalysis N2 - More and more sub-micro and nano-materials shall be "quickly", but accurately characterized with respect to their morphology, shape, size or size distribution, but also to the chemical composition as well by means of an SEM/EDX (Scanning Electron Microscopy / X-Ray Energy Dispersive Spectrometry) system. This undertaking is becoming successful indeed if the transmission mode at low voltages such as those at a SEM, i. e. mostly up to 30 kV, and EDX, respectively is employed. The transmission mode at conventional SEM can e "activated" firstly by re-defining the specimen to be analyzed and preparing it as a thin specimen, which is quasi-transparent to electrons and by finding a way of collecting only the transmitted electrons for analysis. The first issue can be easily realized by using the conventional TEM grids (and the respective sample preparation); the second one becomes feasible either (i) by placing a socalled STEM semiconductor detector directly under the thin specimen or (ii) by using a Special transmission set-up, which enables to the conventional Everhart-Thornley detector to collect only transmitted electrons by blocking the direct collection of secondary electrons and guiding only the transmitted electrons onto it. KW - Microcapsules KW - Nanocapsules KW - Anti-corrosion KW - SEM KW - TSEM KW - Transmission KW - EDX PY - 2013 DO - https://doi.org/10.1017/S1431927613003863 SN - 1431-9276 SN - 1435-8115 VL - 19 IS - Suppl. 2 SP - 374 EP - 375 PB - Cambridge University Press CY - New York, NY AN - OPUS4-29444 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hodoroaba, Vasile-Dan A1 - Akcakayiran, D. A1 - Grigoriev, D.O. A1 - Shchukin, D.G. T1 - Characterization of micro- and nanocapsules for self-heating anti-corrosion coatings by high-resolution SEM with coupled transmission mode and EDX JF - Analyst N2 - The observation of morphological details down to the nanometer range of the outer surface of micro-, submicro- and nanoparticles in a high-resolution scanning electron microscope (SEM) was extended with in-depth observation by enabling the transmission mode in the SEM, i.e. TSEM. The micro- and nanocapsules characterized in this study were fabricated as depots for protective agents to be embedded in innovative self-healing coatings. By combining the two imaging modes (upper and in-depth observation) complementing each other a better characterisation by a more comprehensive interpretation of the 'consistency' of the challenging specimens, e.g. including details 'hidden' beyond the surface or the real specimen shape at all, has been attained. Furthermore, the preparation of the quasi electron transparent samples onto thin supporting foils enables also elemental imaging by energy dispersive X-ray spectroscopy (EDX) with high spatial resolution. Valuable information on the elemental distribution in individual micro-, submicro- and even nanocapsules completes the '3D' high resolution morphological characterization at the same multimodal SEM/TSEM/EDX system. KW - Scanning electron microscopy (SEM) KW - High-resolution transmission in SEM (TSEM) KW - Energy dispersive X-ray spectroscopy (EDX) KW - Micro- and nanocapsules KW - Anti-corrosion coatings PY - 2014 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-304201 DO - https://doi.org/10.1039/c3an01717f SN - 0003-2654 SN - 1364-5528 VL - 139 IS - 8 SP - 2004 EP - 2010 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-30420 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Heeger, Felix A1 - Bourne, E. C. A1 - Wurzbacher, C. A1 - Funke, E. A1 - Lipzen, A. A1 - He, G. A1 - Ng, V. A1 - Grigoriev, I. V. A1 - Schlosser, D. A1 - Monaghan, M. T. T1 - Evidence for Lignocellulose-Decomposing Enzymes in the Genome and Transcriptome of the Aquatic Hyphomycete Clavariopsis aquatica JF - Journal of Funghi N2 - Fungi are ecologically outstanding decomposers of lignocellulose. Fungal lignocellulose degradation is prominent in saprotrophic Ascomycota and Basidiomycota of the subkingdom Dikarya. Despite ascomycetes dominating the Dikarya inventory of aquatic environments, genome and transcriptome data relating to enzymes involved in lignocellulose decay remain limited to terrestrial representatives of these phyla. We sequenced the genome of an exclusively aquatic ascomycete (the aquatic hyphomycete Clavariopsis aquatica), documented the presence of genes for the modification of lignocellulose and its constituents, and compared differential gene expression between C. aquatica cultivated on lignocellulosic and sugar-rich substrates. We identified potential peroxidases, laccases, and cytochrome P450 monooxygenases, several of which were differentially expressed when experimentally grown on different substrates. Additionally, we found indications for the regulation of pathways for cellulose and hemicellulose degradation. Our results suggest that C. aquatica is able to modify lignin to some extent, detoxify aromatic lignin constituents, or both. Such characteristics would be expected to facilitate the use of carbohydrate components of lignocellulose as carbon and energy sources. KW - Aquatic fungi KW - Differential expression KW - Lignocellulose KW - Laccase KW - RNA-Seq PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-536843 DO - https://doi.org/10.3390/jof7100854 VL - 7 IS - 10 SP - 2 EP - 11 PB - MDPI CY - Basel, Schweiz AN - OPUS4-53684 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Selbmann, L. A1 - Benkő, Z. A1 - Coleine, C. A1 - de Hoog, S. A1 - Donati, C. A1 - Druzhinina, I. A1 - Emri, T. A1 - Ettinger, C. L. A1 - Gladfelter, A. S. A1 - Gorbushina, Anna A1 - Grigoriev, I. V. A1 - Grube, M. A1 - Gunde-Cimerman, N. A1 - Karányi, Z. A. A1 - Kocsis, B. A1 - Kubressoian, T. A1 - Miklós, I. A1 - Miskei, M. A1 - Muggia, L. A1 - Northen, T. A1 - Novak-Babič, M. A1 - Pennacchio, C. A1 - Pfliegler, W. P. A1 - Pòcsi, I. A1 - Prigione, V. A1 - Riquelme, M. A1 - Segata, N. A1 - Schumacher, Julia A1 - Shelest, E. A1 - Sterflinger, K. A1 - Tesei, D. A1 - U’Ren, J. M. A1 - Varese, G. C. A1 - Vázquez-Campos, X. A1 - Vicente, V. A. A1 - Souza, E. M. A1 - Zalar, P. A1 - Walker, A. K. A1 - Stajich, J. E. T1 - Shed Light in the DaRk LineagES of the Fungal Tree of Life—STRES JF - Life N2 - The polyphyletic group of black fungi within the Ascomycota (Arthoniomycetes, Dothideomycetes, and Eurotiomycetes) is ubiquitous in natural and anthropogenic habitats. Partly because of their dark, melanin-based pigmentation, black fungi are resistant to stresses including UV- and ionizing-radiation, heat and desiccation, toxic metals, and organic pollutants. Consequently, they are amongst the most stunning extremophiles and poly-extreme-tolerant organisms on Earth. Even though ca. 60 black fungal genomes have been sequenced to date, [mostly in the family Herpotrichiellaceae (Eurotiomycetes)], the class Dothideomycetes that hosts the largest majority of extremophiles has only been sparsely sampled. By sequencing up to 92 species that will become reference genomes, the “Shed light in The daRk lineagES of the fungal tree of life” (STRES) project will cover a broad collection of black fungal diversity spread throughout the Fungal Tree of Life. Interestingly, the STRES project will focus on mostly unsampled genera that display different ecologies and life-styles (e.g., ant- and lichen-associated fungi, rock-inhabiting fungi, etc.). With a resequencing strategy of 10- to 15-fold depth coverage of up to ~550 strains, numerous new reference genomes will be established. To identify metabolites and functional processes, these new genomic resources will be enriched with metabolomics analyses coupled with transcriptomics experiments on selected species under various stress conditions (salinity, dryness, UV radiation, oligotrophy). The data acquired will serve as a reference and foundation for establishing an encyclopedic database for fungal metagenomics as well as the biology, evolution, and ecology of the fungi in extreme environments. KW - Adaptation KW - Black fungi KW - Dothideomycetes KW - Eurotiomycetes KW - Extremophiles KW - Genomics KW - Metabolomics KW - Secondary metabolites KW - Stress conditions KW - Transcriptomics PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-519328 DO - https://doi.org/10.3390/life10120362 VL - 10 IS - 12 SP - 362 PB - MDPI CY - Basel AN - OPUS4-51932 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tleuova, A. A1 - Schenderlein, Matthias A1 - Mutaliyeva, B. A1 - Aidarova, S. A1 - Sharipova, A. A1 - Bekturganova, N. A1 - Miller, R. A1 - Grigoriev, D. T1 - Selection and study of alkoxysilanes as loading in submicrocapsules for self-lubricating coatings JF - Colloids and Surfaces A N2 - The possibility and conditions for the formation of nano- or submicrocapsules loaded with hydrophobic active ingredients (alkoxysilanes) into the matrix of coatings with self-lubricating effect are considered. The optimal composition of the alkoxysilane submicrocapsules, and their physicochemical properties were determined. The longer the radical chain length is, the lower is the rate of hydrolysis, and, accordingly, the more stable is the formed emulsion. The methods of laser correlation spectroscopy in measuring the size and zeta potentials of the submicrocapsules with different loads of the active agent allowed to determine the optimal ratios of the active ingredients. The optimal concentration of the active agent is between 4 and 9%. Based on contact angle studies, octadecyltrimethoxysilane was selected as optimum compound. The introduction of 4 wt% of octadecyltrimethoxysilane reduces the friction coefficient of coatings by 20–30% under vibrating motion and by 15–20% under continuous motion. The data obtained can also be useful for the encapsulation of other hydrophobic active agents and for various other purposes, for example, for the introduction of biocidal agents. KW - Microencapsulation KW - Spontaneous emulsification KW - Self-lubricating coating KW - Antifriction coefficient KW - Alkoxysilanes PY - 2019 DO - https://doi.org/10.1016/j.colsurfa.2018.12.018 SN - 0927-7757 SN - 1873-4359 IS - 563 SP - 359 EP - 369 PB - Elsevier AN - OPUS4-47233 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -